Dispacamide E and other bioactive bromopyrrole alkaloids from two Indonesian marine sponges of the genus Stylissa

Chemical investigation of methanolic extracts of the two Indonesian marine sponges Stylissa massa and Stylissa flabelliformis yielded 25 bromopyrrole alkaloids including 2 new metabolites. The structures of all isolated compounds were unambiguously elucidated based on extensive 1D and 2D NMR, LR-MS and HR-MS analyses. All isolated compounds were assayed for their antiproliferative and protein kinase inhibitory activities. Several of the tested compounds revealed selective activity(ies) which suggested preliminary SARs of the isolated bromopyrrole alkaloids.     

Long-chain alkanoic acid esters of lupeol from Dorstenia harmsiana Engl. (Moraceae)

In addition to lupeol (1a), three long-chain alkanoic acid esters of lupeol, in which two were new, were isolated from the hexane and ethyl acetate twigs extract of Dorstenia harmsiana Engl. (Moraceae). The structures of the new compounds were elucidated on the basis of 1D and 2D NMR experiments. Some isolated compounds were evaluated for their antimicrobial activities. The lupeol and its three long-chain alkanoic acid esters showed antifungal and antibacterial activities.     

Lipid constituents of Trifolium resupinatum var. microcephalum

The two new compounds (3-methyl-1-nonene-3-ol and 2',3'-dihydroxy propyl pentadecanoate) and four known compounds were isolated from Trifolium resupinatum L. var. microcephalum Zoh. (Leguminosae). All the compounds were reported for the first time from this plant. The stuructures of the isolates were determined by 1D, 2D NMR techniques and MS spectroscopy.     

DNA damaging activities of methanol extract of Ajuga postii and iridoid glucoside reptoside

An iridoid glucoside reptoside (1) has been isolated as a DNA damaging active agent by bioassay-guided fractionation of the methanol extract of Ajuga postii. Furthermore, from the acetone extract of A. postii two known triterpenic compounds ursolic acid, alpha-amyrin and two steroidal compounds (24S)-24-ethylcholesta-5,25-dien-3beta-ol and beta-sitosterol were isolated. Their structures were elucidated based on 1D and 2D NMR techniques and mass data.     

Iridoids from Neopicrorhiza scrophulariiflora and their hepatoprotective activities in vitro

Four new non-glycosidic iridoids, piscrocins D (1), E (2), F (6), and G (7), as well as two new iridoid glycosides, piscrosides A (8) and B (9), were isolated from the roots of Neopicrorhiza scrophulariiflora (Scrophulariaceae), together with seven known iridoids. The structures of the isolated compounds were established by means of 1D and 2D NMR spectroscopy and chemical methods. The hepatoprotective activities of these compounds were evaluated by measuring their effects on CCl(4)-induced hepatocytes damage in vitro, and the structure-activity relationships were also discussed.     

Steroidal saponins and pregnane glycosides from Smilax microphylla

Six steroidal saponins and two pregnane glycosides were isolated from the BuOH subfraction of 70% EtOH extract of Smilax microphylla C.H.Wright, among them two were new compounds (1 and 7). Pregnane glycosides were firstly isolated from the genus Smilax (Smilacaceae). Structures of the new compounds were determined on the basis of HR‐ESI‐MS, 1D and 2D NMR spectroscopic analysis. Copyright © 2012 John Wiley & Sons, Ltd.     

Two compounds from the endophytic Colletotrichum sp. of Ginkgo biloba

Two compounds, apigenin-8-C-beta-D-glucopyranoside and 2-(hydroxymethylthio)ethanol, were extracted from the fermentation products of a strain of endophytic fungus, Colletotrichum sp. NTB-2, isolated from the leafstalk of Ginkgo biloba. The structures of the two compounds were determined on the basis of extensive spectroscopic analysis, including 1D and 2D NMR spectral data. The compounds wereobtained from microorganisms for the first time.     

Two new secolignans from the roots of Urtica mairei Levl.

Twonew secolignans(1 and 2)were isolated from the roots of Urtica mairei Levl.The structures of these two compounds were determined on the basis of extensive spectroscopic methods including HR-E1-MS.1D and 2D NMR experiments.     

Two new secolignans from the roots of Urtica mairei Levi.

Two new secohgnans(1 and 2)were isolated from the roots of Urtica mairei Levi.The structures of these two compounds were determined on the basis of extensive spectroscopic methods including HR-EI-MS,1D and 2D NMR experiments.     

锦灯笼果实的化学成分研究

从锦灯笼中分离得到5个甾体类化合物,分别鉴定为酸浆素A(1,alkekengilin A)、酸浆素B(2,alkekengilin B)、酸浆苦素D(3,physalin D)、酸浆苦素P(4,physalin P)、4,7-二去氢新酸浆苦素B(5,4,7-didehydro-neophysalin B).其中酸浆素A(1,alkekengilin A)和酸浆素B(2,alkekengilin B)为新的天然产物.运用现代波谱技术对上述化合物的结构进行了确证.     

Rhusemialins A-C, new cyclolignan esters from the roots of Rhus javanica var. roxburghiana

Three new cyclolignan esters and seven known compounds including two cyclolignan isolariciresinol, lynoiresinol, and five aromatic compounds methyl ferulate, vanillin, 4-hydroxy-3,5-dimethoxybenaldehyde, 4-methoxygallic acid, gallic acid were isolated from n-butanol extract of Rhus semialata (Anacardiaceae). The structural elucidations of rhusemialins A (1), B (2), and C (3) were based on FAB-MS, 1D and 2D NMR spectra.     

Triterpene derivatives from Abies spectabilis leaves of Nepalese origin

Our ongoing studies of Nepalese medicinal plants has led to the isolation and characterization of five new triterpenes, two known triterpenes and two phenolic derivatives from Abies spectabilis (D.Don) Mirb leaves grown in the high mountain. The structures of the isolated compounds were characterized by means of 1D and 2D NMR spectroscopic and MS techniques.     

Two New Retigerane‐Type Sesterterpenoids from the Lichen Leprocaulon microscopicum

Two new sesterterpenes, 1 and 2 , have been isolated from the lichen Leprocaulon microscopicum. In addition to classic chromatographic methods, a liquid‐liquid chromatography technique, namely centrifugal partition chromatography (CPC) was applied for the purification of compound 2 . The structures were determined by analyses of mass spectrometry and 1D‐ and 2D‐NMR data. The relative configuration of the isolated compounds was assigned on the basis of 2D‐NOESY experiments. The two compounds possess a rare pentacyclic carbon skeleton typical for lichen metabolism, and quite unusual in the vegetal kingdom.     

The isolation and antimicrobial activities of three monocylic sesquiterpenes from Syringa pinnatifolia

We report the isolation of a new sesquiterpene, named as syripinol (1), and two known compounds from the volatile oil of the stems of Syringa pinnatifolia, for the first time. The structures of the isolated compounds were elucidated on the basis of spectroscopic methods including UV, IR, ESI-MS, 1D and 2D NMR techniques. The antibacterial activities of the compounds were also evaluated against various bacteria which showed good results.     

Studies on Chemical Constituents of Salix Matsudana

Two new phytane‐type diterpene‐γ‐lactones named Hanliuin III ( 3 ) and Hanliuin IV ( 4 ), together with two previously reported diterpenes, six known flavonoids and five other known compounds were isolated from Salix matsudana. The structures of two new compounds were deduced from spectroscopic methods and 2D NMR techniques.     

Hyloglyceride and hylodiglyceride: two new glyceride derivatives from Hylodendron gabunensis

Phytochemical investigation of Hylodendron gabunensis resulted in the isolation of two new glyceride derivatives, hyloglyceride (1) and hylodiglyceride (2). The structures of the two new compounds were determined by comprehensive analysis of their 1D and 2D NMR spectroscopic, and HREIMS data. One known compound was also isolated and identified as beta-sitosterol.     

Glutinoin, a novel antioxidative ellagitannin from Alnus glutinosa cones with glutinoic acid dilactone moiety

The crude aqueous ethanol extract of the cones of Alnus glutinosa (L.) Gaertn. (Betulaceae; black alder, European alder) was obtained and further partitioned between water and various organic phases. The active water and butanol phases were subjected to assay-guided (DPPH) fractionation using repetitive RP HPLC until individual compounds were isolated. Their antioxidative activities, measured as SC?? values, were evaluated. The chemical structures of the isolated compounds were elucidated with the help of mass spectroscopy, (1)H NMR technique, UV spectroscopy, and chemical approaches. One novel ellagitannin, glutinoin (2), along with two known compounds, pedunculagin (1) and praecoxin D (3), were isolated and found to contribute to antioxidative activity of the A. glutinosa cones extract. The activities (SC??) of 1-3 were evaluated as 0.95 (1), 1.00 (2) and 1.01?μg?mL?1 (3). The scavenging effects of glutinoin (2) and praecoxin D (3) were reported for the first time.     

A novel diarylheptanoid bearing flavonol moiety from the rhizomes of Alpinia officinarum Hance

<正>A novel diarylheptanoid bearing flavonol moiety,named officinin A(1),along with two known compounds galangin and kaempferide were isolated from the rhizomes of Alpina officinarum Hance.The structure elucidation was accomplished by HRESI -MS,1D and 2D NMR methods.     

Two new secolignans from the roots of Urtica mairei Lev1.

Two new secolignans （1 and 2） were isolated from the roots of Urtica mairei Levi. The structures of these two compounds were determined on the basis of extensive spectroscopic methods including HR-EI-MS, 1D and 2D NMR experiments.     

Complete 1H and 13C data assignments of two new guaianolides isolated from Ainsliaea fragrans

Two new guaianolides, 8alpha-hydroxy-11alpha,13-dihydroglucozaluzanin C and 11alpha,13-dihydroglucozaluzanin C, were isolated from Ainsliaea fragrans, along with two known guaianolides, 8alpha-hydroxy-11alpha,13-dihydrozaluzanin C and glucozaluzanin C. The structures of the new compounds were unambiguously established by HR-ESI-MS, one-dimensional (1D) (DEPT), two-dimensional (2D) ((1)H-(1)H COSY, HSQC, HMBC, ROESY) NMR experiments and by comparison with structurally related compounds. The known compounds were identified by comparison of spectral data with published references. Some NMR data of the known compounds were reported for the first time.