共查询到10条相似文献,搜索用时 176 毫秒
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Metabolomics,peptidomics and proteomics applications of capillary electrophoresis-mass spectrometry in Foodomics: A review 总被引:1,自引:0,他引:1
Clara IbáñezAuthor Vitae Carolina SimóAuthor VitaeVirginia García-CañasAuthor Vitae Alejandro CifuentesMaría Castro-PuyanaAuthor Vitae 《Analytica chimica acta》2013
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Identifying and quantifying in a high throughput manner the proteins expressed by cells, tissues or an organism provides the basis for understanding the functions of its constituents at a "systems" level. As a result, proteome analysis has increasingly become the focus of significant interest and research over the past decade. This is especially true following the recent stunning achievements in genomics analyses. However, unlike the static genome, the complexities and dynamism of the proteome present significant analytical challenges and demand highly efficient separations and detection technologies. A number of recent technological advancements have been in direct response to these challenges. Currently, strategically mated combinations of sophisticated separations techniques and advanced mass spectrometric detection represent the best approach to addressing the intricacies of the proteome. Liquid-phase separations, often within capillaries, are increasingly recognized as the best separations technique for this approach. In combination on-line with mass spectrometry, liquid-phase separations provide the improved analytical sensitivity, sample throughput, and quantitation capabilities necessitated by the multifaceted problems within proteomics analyses. This review focuses primarily on current high-efficiency capillary separations techniques, including both capillary liquid chromatography and capillary electrophoresis, applied to the analysis of complex proteomic samples. We emphasize developments at our laboratory and illustrate technical advances that attempt to review the role of separations within the broader context of a state-of-the-art integrated proteomics effort. 相似文献
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In this article we discuss current trends in the techniques available for plant metabolomics. Chemical assignment of unknown metabolites leads to understanding of biosynthetic mechanisms at the gene level for genome-sequenced plants. Metabolomics using mass spectrometry has achieved innovative results in phytochemical genomics for primary and secondary metabolism in the model plant Arabidopsis thaliana by using publicly and commercially available information and standard compounds. However, finding a consolidated analytical technique for elucidation of structural information (e.g., elemental composition and structure) remains challenging. Recently, hyphenated analytical techniques and computer-assisted structural analysis with high-throughput and high-accuracy have been developing. Metabolite-driven approaches using such technology will be of central importance in phytochemical genomics. 相似文献
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The Orbitrap mass analyzer has become a mainstream mass spectrometry technique. In addition to providing a brief introduction to the Orbitrap technology and its continuing development, this article reviews the most recent publications quoting the use of the Orbitrap detection for a variety of chromatographic separation techniques. Its coupling to reversed-phase liquid chromatography (LC) represents undoubtedly the most ubiquitous approach to both small molecule and proteomic analyses. Multi-dimensional LC separations have an important role to play in the proteomics applications while an ultra-high-pressure LC is more frequently encountered in the area of metabolomics and metabolite analysis. Recently, special chromatographic techniques such as hydrophilic interaction chromatography and its variations have also been also cited with the Orbitrap detection. 相似文献
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From proteomics to genomics 总被引:7,自引:0,他引:7
Sperling K 《Electrophoresis》2001,22(14):2835-2837
Presently, science is moving from genomics to proteomics in order to get insight into the functional network of gene expression. Actually however, proteomics is much older than genomics and dates back to the introduction of the two-dimensional gel electrophoresis technique (2-DE) independently by Klose and O'Farrell. Based on this approach almost all cellular proteins can be separated. New developments in mass spectrometry allowed identification of single spots in the 2-DE protein pattern, including the underlying genes. Joachim Klose has focused his pioneering 2-DE studies on mouse models with special emphasis on quantitative protein variants. According to him, proteins are living molecules exhibiting a characteristic protein phenotype. 相似文献
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Benjamin P. Bowen Trent R. Northen 《Journal of the American Society for Mass Spectrometry》2010,21(9):1471-1476
Metabolomics is the comprehensive profiling of the small molecule composition of a biological sample. Since metabolites are
often the indirect products of gene expression, this approach is being used to provide new insights into a variety of biological
systems (clinical, bioenergy, etc.). A grand challenge for metabolomics is the complexity of the data, which often include
many experimental artifacts. This is compounded by the tremendous chemical diversity of metabolites. Identification of each
uncharacterized metabolite is in many ways its own puzzle (compared with proteomics, which is based on predictable fragmentation
patterns of polypeptides). Therefore, effective data reduction/prioritization strategies are critical for this rapidly developing
field. Here we review liquid chromatography electrospray ionization mass spectrometry (LC/MS)-based metabolomics, methods
for feature finding/prioritization, approaches for identifying unknown metabolites, and construction of method specific ‘Metabolite
Atlases’. 相似文献
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