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1.
利用反相微乳液法制备了Ru(bpy)2+3-SiO2复合纳米粒子(RuSiO2NPs),采用Nafion/多壁碳纳米管(MWCNTs)复合膜技术,实现了对RuSiO2NPs有效而稳定的固定,从而制备了电化学发光传感器,在0.1mol/L磷酸盐缓冲溶液(PBS,pH=7.4,含50mmol/L三正丙胺)介质中,实现了对双酚A(BPA)的免标记检测。在优化实验条件下,电化学发光强度减少值与BPA浓度的负对数在1.0×10-11~1.0×10-7 mol/L范围内呈良好的线性关系,相关系数R=0.9978,检出限为8.0×10-12 mol/L。对1.0×10-9 mol/L BPA平行测定11次,其相对标准偏差为2.7%。实验结果表明该传感器具有良好的稳定性和重现性。  相似文献   

2.
先采用滴涂法制备了石墨烯修饰电极(GR/GCE),然后采用电化学方法将纳米金沉积于石墨烯表面制备了纳米金/石墨烯复合材料修饰电极(Au NPs/GR/GCE)。研究了异烟肼(isoniazid,INZ)在该Au NPs/GR/GCE上的电化学行为。结果表明,异烟肼在该修饰电极上有良好的电化学响应。在优化条件下,线性扫描伏安法测定异烟肼的线性范围为1.0×10-7~1.0×10-4mol/L,检出限为5.0×10-8mol/L(S/N=3)。用该法测定了异烟肼注射液中异烟肼的含量,结果令人满意。  相似文献   

3.
本文以酸化碳布(CC)作为柔性工作电极,采用恒电位沉积法在碳布上修饰纳米Au-Cu复合粒子(Au-Cu NPs),以四环素(TC)为模板分子,邻苯二胺为功能单体,采用循环伏安法在修饰了纳米粒子的活化碳布上电聚合分子印迹膜,制备了四环素分子印迹柔性传感器(Cu-Au NPs/PANI/TC/CC),通过循环伏安法(CV)、差分脉冲伏安法(DPV)研究了其电化学性能。结果显示,在优化实验条件下,TC的DPV峰值电流与其浓度在1.0×10~(-7)~3.0×10~(-5) mol/L范围内成良好的线性关系,相关系数为0.9996,检出限为2.57×10~(-11) mol/L,实际样品的加标回收率为97.3%~105%。该传感器柔软稳定,灵敏度高,抗干扰性和重复性好,可应用于柔性可穿戴传感器的设计。  相似文献   

4.
在金电极表面滴涂石墨烯(GR),通过电沉积技术沉积纳米金(Au)构成石墨烯-纳米金修饰电极(Au-GR/GE)。以芦丁为模板分子,邻氨基酚为功能单体,通过电聚合反应在Au-GR/GE表面合成一种对芦丁具有特异性识别能力的分子印迹传感器膜(MIP)。采用循环伏安法(CV)、差分脉冲伏安法(DPV)研究了印迹膜的性能、结构和分子印迹效应,并与槲皮素进行了选择性响应的比较,发现此传感器对芦丁具有良好的选择性。在最佳实验条件下,其对芦丁浓度的定量测定线性范围为6.30×10-7~1.70×10-4mol/L,线性方程为I(μA)=3.8136-8.6247 lg c(mol/L),R=0.9961,检出限为2.10×10-7mol/L。  相似文献   

5.
以速灭威(MTMC)为模板分子,甲基丙烯酸(MAA)为功能单体,马来松香丙烯酸乙二醇酯(EGMRA)为交联剂,在石墨烯掺杂金纳米粒子修饰玻碳电极表面合成分子印迹膜,研制了测定MTMC的分子印迹电化学传感器。采用扫描电镜(SEM)对传感膜的形貌进行了表征,通过循环伏安法(CV)、电化学阻抗谱法(EIS)和差示脉冲伏安法(DPV)对传感器的性能进行了研究。DPV测试表明,MTMC的浓度在1.0×10-7~1.0×10-4mol/L范围内呈现良好的线性关系(线性相关系数为R=0.9936),检出限2.9×10-8mol/L(S/N=3)。传感器应用于蔬菜样品的加标回收检测,回收率在93.4%~106.4%之间。  相似文献   

6.
以邻苯二胺为功能单体,尼泊金乙酯为模板分子,通过电化学聚合在玻碳电极表面制备了尼泊金乙酯分子印迹膜,采用循环伏安法及方波伏安法,以K3[Fe(CN)6]为电活性探针,建立了间接测定尼泊金乙酯的分析方法。实验结果表明,制备的分子印迹膜电化学传感器对尼泊金乙酯具有较高的选择性和灵敏度,测定尼泊金乙脂的线性范围为2.5×10-6~1.0×10-5 mol/L,检出限为8.61×10-8 mol/L。  相似文献   

7.
王伟  李娟  白茹  韩珍  冯雪薇  孙越 《应用化学》2020,37(5):595-603
在金电极表面,用无金属可见光诱导原子转移自由基聚合(MVL ATRP)的方法制备聚丙烯酰胺@氧化石墨烯/纳米钯复合物修饰电极(Au/PAM@GO/Pd)。采用电化学循环伏安法(CV)、交流阻抗法(EIS)、扫描电子显微镜(SEM)、能量色散X射线光谱法(EDS)对Au/PAM@GO/Pd电极进行表征,结果表明在金电极表面成功制备了复合物。利用Au/PAM@GO/Pd电极作为电化学传感器,该传感器能成功地检测溶液中的乙醇。在最佳条件下,利用差分脉冲伏安法(DPV)该传感器检测乙醇的线性范围为1.0×10-8~1.0 mol/L,检出限(S/N=3)为1.3×10-9 mol/L,线性相关系数为0.996。  相似文献   

8.
以水杨酸(SA)为模板分子,邻苯二胺(o-PPD)及吡咯(Py)为复合功能单体,在石墨烯修饰的玻碳电极表面制备分子印迹电化学传感器(MIP/GO/GCE),用扫描电镜(SEM)观察印迹膜的表面形貌,方波伏安法(SWV)和循环伏安法(CV)对分子印迹传感器的性能进行表征。通过优化实验条件,显示SA浓度在1.0×10-8~1.0×10-2 mol/L范围内,分子印迹传感器峰电流与SA浓度负对数具有良好的线性关系,检出限为8.6×10-9 mol/L。该传感器对SA具有良好的选择性,样品回收率为101%~106%,相对标准偏差(RSD)为3.8%。SA分子印迹传感器的制备简单、抗干扰性好、灵敏度高、成本低廉,具有较好实用价值。  相似文献   

9.
基于石墨烯分子印迹电化学传感器测定芦丁   总被引:2,自引:0,他引:2  
将石墨烯(GR)滴涂至裸Au电极表面,并以邻氨基酚为功能单体,芦丁为模板分子,制备了芦丁分子印迹膜电化学传感器,利用循环伏安法(CV)和差分脉冲伏安法(DPV)对制得的传感器进行了电化学性能研究,并且对制备条件和测定条件进行了优化。结果表明,与裸Au电极相比,该GR修饰的Au电极在[Fe(CN)_6]~(3-/4-)溶液中峰电流明显增大,显著提高了芦丁分子印迹传感器的灵敏度。在最优实验条件下,基于GR分子印迹电化学传感器在4.40×10~(-6)~2.80×10~(-4) mol/L范围内呈良好的线性关系,检测限为1.46×10~(-6) mol/L。用该传感器测定了黑茶中芦丁的含量,获得较好结果。  相似文献   

10.
基于不同聚集态金纳米粒子(Au NPs)对罗丹明B(Rh B)的荧光猝灭作用,建立了一种简单、灵敏、快速测定药物甲巯咪唑的新方法。初步探讨了方法机理,并对p H值、反应时间、Au NPs和Rh B的浓度等实验条件进行了优化。优化实验条件下,方法的线性范围为4.38×10-8~0.876×10-5mol/L,检出限(S/N=3)为3.30×10-8mol/L。该法用于甲巯咪唑药品中甲巯咪唑的测定,获得了满意结果。  相似文献   

11.
A novel electrochemical sensor based on the immobilization of tyrosinase(tyr) onto gold nanoparticles(nano-Au) and thioctic acid amide(T-NH2) self-assembled monolayers(SAMs)-modified gold electrode has been developed for the determination of bisphenol A(BPA).It was found that the nano-Au could significantly enhance the electrochemical response of tyr/nano-Au/T-NH2/Au electrode to BPA,and the enhancement effect of nano-Au on the current response was also related to the enzyme.The results indicated that the biosensor could be used as a detector for BPA determination with a linear range from3.99 ×10-7mol/L to 2.34 ×10-4mol/L and a detection limit of 1.33×10-7mol/L.In addition,this biosensor showed good reproducibility.  相似文献   

12.
In this study, we synthesized molybdenum disulfide/polyaniline (MoS2/PANI) nanocomposite via in situ polymerization of aniline in the presence of thin-layered MoS2. The as-prepared MoS2/PANI nanocomposite obtained an improved electrochemical performance due to the physisorption interaction between aromatic aniline and the basal plane of MoS2. Furthermore, we constructed a new kind of electrochemical sensor based on MoS2/PANI nanocomposite for the detection of chloramphenicol, which showed an excellent performance. The sensor has a high sensitivity and wide detection range from 1×10-7 mol/L to 1×10-4 mol/L, with a low detection limit of 6.9×10-8 mol/L.  相似文献   

13.
In this work,a metal-organic frameworks material MIL-88 was prepared easily using solvent-thermal method,and was first found to have catalytic activities similar to those of biological enzymes such as catalase and peroxidase.The material was characterized by XRD,SEM,TEM,EDX,FT-IR techniques and an N_2 adsorption method.It exhibited peroxidase-like activity through catalytic oxidation of the peroxidase substrate 3,3',5,5'-tetramethylbenzidine(TMB) in the presence of H_2O_2,producing a blue-colored solution.Under optimal conditions,the absorbance at 652 nm is linearly correlated with the concentration of H_2O_2 from 2.0×10~(-6) mol/L to 2.03×10~(-5) mol/L(R~2=0.981) with a detection limit of 5.62×10~(-7) mol/L(S/N=3).More importantly,a sensitive and selective method for ascorbic acid detection was developed using this material as a catalyst.The analytical method for ascorbic acid detection was observed to have a linear range from 2.57×10~(-6) mol/L to 1.01×10~(-5) mol/L(R~2=0.989) with a detection limit of 1.03×10~(-6) mol/L(S/N=3).This work suggests MOFs have advantages of preparing biomimetic catalysts and extends applications of the functional MOFs in the field of biosensor.  相似文献   

14.
A new method has been proposed to realize the visual detection of D-amino acids(DAAs) via the antiaggregation of 4-mercaptobenzoic acid modified gold nanoparticles(AuNPs) in the presence of D-amino acid oxidase(DAAO). The negatively charged AuNPs were prepared using sodium citrate as a reducer and stabilizer. The presence of 4-mercaptobenzoic acid(4-MBA) and Cu2+induces the aggregation of AuNPs,resulting in a color change from ruby red to royal purple. However, DAAO could oxidize DAAs to generate H2O2. In the presence of H2O2, the mercapto(–SH) group in 4-mercaptobenzoic acid can be oxidized to form a disulfide(–S–S–) bond. Based on these facts, the pre-incubation of DAAs and 4-mercaptobenzoic acid with DAAO would significantly reduce the concentration of free 4-mercaptobenzoic acid molecules,thus the aggregation of AuNPs was interrupted since due to the lack of inducer. As the concentration of DAAs increases, the color of the AuNPs solution would progress from royal purple to ruby red.Consequently, DAAs could be monitored by the colorimetric response of AuNPs using a UV–vis spectrophotometer or even naked eyes. This DAAO mediated visual detection method could determine Dalanine(D-Ala) as a representative DAA with concentrations ranging from 1.5×10~(-7)mol L~(-1) to 3.0×10~(-5)mol L~(-1), and the detection limit was as low as 7.5×10~(-8)mol L~(-1). The proposed method is convenient, low-cost and free of complex equipment, making it feasible to analyze the concentration of D-Ala in real samples of b-amyloid peptide(Aβ1–42).  相似文献   

15.
以三氟氯氰菊酯为模板分子,邻苯二胺和邻氨基苯酚为复合功能单体,通过电聚合法制备了能够特异识别模板分子及其结构类似物的分子印迹电化学传感器。 采用循环伏安法和方波伏安法优化了制备和检测条件,对传感器的印迹效应和分析性能进行了研究,并建立了农产品中三氟氯氰菊酯农药残留的快速分析方法。 在最佳条件下,三氟氯氰菊酯在1.0×10-7~1.0×10-5 mol/L浓度范围内线性关系良好,检出限(S/N=3)为3.0×10-8 mol/L,样品加标平均回收率在84.8%~94.7%之间,相对标准偏差(RSD)为1.1%~4.8%(n=5)。 该传感器对三氟氯氰菊酯以及结构类似物有良好的选择性、稳定性和重现性,而且操作简便、检测快速、灵敏、成本低,具有良好的应用前景。  相似文献   

16.
In this work a PVC membrane electrode based on bis(N-3-methyl phenyl salicylidenaminato)copper(II)as ionophore was prepared.The electrode was tested by inorganic anions and showed good selectivity for thiocyanate ion.This sensor showed Nerstian behavior with a slope of a-59.3 mV per decade at 25℃.The proposed electrode exhibited a wide linear range from 1.0 × 10~(-6) mol/L to 1.0× 10~(-1) mol/L with a detection limit of 5.0×10~(-7) mol/L.The electrode response was independent of pH in the range of 4.0-10.0.The response time is about 9-21s,and the electrode can be used for over 60 days without considerable deterioration.The prepared sensor was applied as an indicator electrode in potentiometric titration of SCN with Ag~+ ion and to determine the thiocyanate in samples of urine and saliva.  相似文献   

17.
The macrocyclic ligand,15-crown-5,was used as an ionophore for fabrication of a polyvinyl chloride(PVC) based membrane sensor for Ag(Ⅰ) cation.For construction of the Ag(Ⅰ) cation selective electrode the best response characteristics were obtained using the composition: 15-crown-5/PVC/o-nitrophenyloctylether(NPOE)/sodium tetraphenyl borate(NaTPB) in the percentage ratio of 5.6/30/60.5/3.9(w/w/w/w).The electrochemical sensor shows a linear dynamic range 1.0 10 7–1.0 10 1mol/L and a Nernstian slope of 58.9 0.5 mV/decade with a detection limit of 8.09 10 8mol/L for Ag(Ⅰ) cation.It has a fast response time of 10 s and can be used for at least 8 weeks without any considerable divergences in its potential response.It was successfully used as an indicator electrode in potentiometric titration of Ag(Ⅰ)cation with I and Cl anions and also for the determination of this metal cation in radiology waste water.  相似文献   

18.
In this work a new Lu3+ PVC-membrane sensor based on 2,2’-dithiobis(4-methylthiazole)(TMT) has been fabricated.The sensor exhibits a Nernstian slope of 19.6±0.4 mV decade(?) in the concentration range of 1.0×106-1.0×102 mol L1 and a detection limit of 6.8×107 mol L1.It could work well in the pH range of 2.7-9.6.The selectivity of the sensor against a lot of common alkaline,alkaline earth,transition,heavy metals and specially lanthanide ions was very good.  相似文献   

19.
合成了一种新型的,能在含水介质中比色荧光双通道单一选择性识别CN-的传感器分子1-羟基萘甲叉酰肼乙基-3-羟基萘甲叉酰肼甲基苯并咪唑溴鎓盐(J1)。在J1的DMSO/H2O(体积比3∶2)HEPES的缓冲体系(pH=7.2)中分别加入F~-、Cl~-、Br~-、I~-、AcO~-、HSO_4~-、ClO_4~-、H_2PO_4~-、SCN~-和CN~-等阴离子后,只有CN-的加入会使得溶液颜色发生明显的变化,由无色变为深黄色。相应地在J1的DMSO/H2O(体积比4∶1)HEPES的缓冲体系(pH=7.2)中加入CN~-,溶液发出明亮的黄色荧光。这一识别过程,不会受到其它阴离子的干扰。紫外-可见光谱的最低检测限为1.57×10~(-7)mol/L,检测线性范围为3.875×10~(-4)~2.15×10~(-2)mol/L。荧光光谱的最低检测限为4.63×10~(-6)mol/L,检测线性范围为0.8×10~(-4)~1.60×10~(-3)mol/L。此结果表明,J1是一种良好的用于识别CN-的化学传感器,在含水介质中对CN-具有选择性好、灵敏度高以及抗干扰性强的识别性能。与此同时,基于J1对于CN-的高选择性识别我们制备了CN-的检测试纸,该试纸能够方便、快捷、准确地检测水中的CN~-。  相似文献   

20.
制备了一种对Pb(Ⅱ)、Cd(Ⅱ)和Cu(Ⅱ)等多金属离子具有灵敏、选择性响应的新型印迹传感器。 以甲基丙烯酸与乙二胺四乙酸分别为功能单体与配体,乙二醇二甲基丙烯酸酯为交联剂制备出多金属离子印迹聚合物。 采用滴涂法将离子印迹聚合物均匀地修饰至氧化石墨烯/富勒烯复合材料修饰碳电极表面,成功制备出灵敏的多金属离子印迹电化学传感器。 采取循环伏安法、交流阻抗和差分脉冲法等技术对印迹电化学传感器的性能进行表征,结果表明该多金属离子印迹电化学传感器具有良好的选择性。 在1.0×10-9~5.0×10-7 mol/L范围内,该多金属离子印迹电极的响应电流与金属离子浓度呈现良好的线性关系,对Pb(Ⅱ)、Cd(Ⅱ)和Cu(Ⅱ)金属离子的最低检测限分别为5.0×10-10、5.0×10-10和1.0×10-10 mol/L。 该多金属离子印迹电化学传感器成功用于实际样品中微量Pb(Ⅱ)、Cd(Ⅱ)和Cu(Ⅱ)等金属离子的检测。  相似文献   

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