色谱最新研究亮点——新型功能化色谱分离介质的研究进展

整体柱由于具有制备方法简单、通透性好、柱容量大、易实现快速分离的优点,近年来在生物大分子的高效、快速、高通量分离分析方面得到了较快的发展。但是由于有机聚合物整体柱的表面积小（只有几十平米/g）,内部结构不均一,因此还存在分离小分子物质柱效低的缺点。最近,人们在努力寻求一种较为完美的低压高效的功能化色谱分离介质的制备方法,以期能够在大分子和小分子等多种物质的分离分析中得到普遍应用。     

硅胶整体柱的制备与应用研究进展

整体柱是近年来发展起来的一种新型色谱分离介质.其中硅胶基质整体柱具有理想的机械强度,通透性好,及在有机溶剂条件下稳定等优点,在分离科学中有良好的应用前景.本文结合近年来硅胶整体柱的研究成果,从制备方法及应用角度作了综述,并对这一领域的未来发展进行了展望.     

高效液相色谱整体柱在药物分离分析中的应用进展

本文对高效液相整体柱在药物分离分析方面的应用进行了综述.主要介绍了以烷氧基硅烷为主要原料,采用溶胶-凝胶法制备的硅胶整体柱,由于其具有微米级通孔结构和大的比表面积,他们在高效、快速分离小分子物质方面得到广泛地应用.对于聚合物整体柱,主要介绍了包括分子印迹聚合物在内的有机聚合物整体柱在药物分离、生物样品的处理等方面的应用.     

有机聚合物整体柱及其在生物样品分离分析前处理中的应用

整体柱由于具有制备简单、内部结构均匀、重现性好、柱效较高和可进行快速分离等优点,在分离、催化等众多领域具有良好的应用前景。 本文综述了近年来有机聚合物整体柱在生物样品分离分析中的应用,并对其存在的问题和发展趋势进行了讨论。     

APBA阳离子交换整体柱的制备及在蛋白质分离复性中的应用

整体柱是近年来发展迅速的一种具有传质速率快、稳定性好、制备简单等多项优点的色谱固定相,离子交换柱是生物大分子色谱分离中很重要的一类同定相.     

有机聚合物整体柱的改性与应用进展

有机聚合物整体柱具有制备简单、易于改性和通透性好的优点,不仅可作为微柱色谱分离分析的固定相,而且可用作样品纯化富集的载体,广泛应用于基体复杂的生化样品分析领域.该文综述了近年来有机聚合物整体柱的研究进展,重点介绍了其制备方法、改性技术及在生化样品预处理领域的研究动态.针对其制备方法,从制备过程、制备原料(单体、交联剂、...     

毛细管整体柱的制备技术及其应用进展

毛细管整体柱是以其制备相对简单无需烧结塞子,渗透性好,柱效高,低柱压等优点,成为目前备受关注的液相色谱固定相.它具有较好的重现性,可进行快速分离,已被应用于毛细管电色谱(CEC)和微柱高效液相色谱(μ-HPLC).本文主要介绍近几年毛细管整体柱的制备技术及其应用概况.     

新型功能化色谱分离介质的研究进展

整体柱由于具有制备方法简单、通透性好、柱容量大、易实现快速分离的优点,近年来在生物大分子的高效、快速、高通量分离分析方面得到了较快的发展。但是由于有机聚合物整体柱的比表面积小(每克只有几十平方米)、内部结构不均一,因此还存在分离小分子物质时柱效低的缺     

“一锅法”制备C18-硅胶杂化毛细管整体柱及其应用

本文发展了一种“一锅法”制备C18-硅胶杂化毛细管整体柱的方法。发展了一种“一锅法”制备有机-无机杂化毛细管整体柱的方法。首先将四甲氧基硅烷、乙烯基三甲氧基硅烷在弱酸条件下水解,然后加入有机单体(甲基丙烯酸-N,N-二甲基十八烷基溴化铵乙酯)及自由基聚合引发剂,同步原位完成硅羟基间的缩聚反应及双键间的自由基聚合反应,从而制成C18-硅胶杂化整体柱;同时用毛细管电色谱(CEC)和毛细管液相色谱(CLC)对其柱效和分离能力进行了初步评价,并将其应用到微柱液相色谱-串联质谱对牛血清白蛋白酶解液的分离分析。结果表明,该C18-硅胶杂化整体柱具有较高的柱效和较好的重现性,在分离分析复杂生物样品方面具有较大的应用前景。此外,本文所发展的方法在制备杂化整体柱的过程中不借助相应的硅烷试剂,为杂化整体柱的制备提供了一种新的思路。     

温敏性整体柱的制备及应用研究进展

整体柱是一种用有机或无机聚合方法在色谱柱内进行原位聚合的连续床固定相。将温敏凝胶引入到整体柱体系中制备得到的温敏性整体柱,不仅可以使分离过程调控简单,还可以避免有机溶剂的使用,使整个分离过程绿色环保。文章综述了温敏-普通整体柱、温敏-毛细管柱、温敏-微流控柱、温敏-印迹柱和温敏-磁性柱的制备方法,以及近十五年来温敏性整体柱在核酸、类固醇、金属离子和其他物质分离、富集等方面中的应用。     

Monolithic columns in high-performance liquid chromatography

Monolithic media have been used for various niche applications in gas or liquid chromatography for a long time. Only recently did they acquire a major importance in high-performance column liquid chromatography (HPLC). The advent of monolithic silica standard- and narrow-bore columns and of several families of polymer-based monolithic columns has considerably changed the HPLC field, particularly in the area of narrow-bore columns. The origin of the concept, the differences between their characteristics and those of traditional packed columns, their advantages and drawbacks, the methods of preparation of monoliths of different forms, and the current status of the field are reviewed. The actual and potential performance of monolithic columns are compared with those of packed columns. Monolithic columns have considerable advantages, which makes them most useful in many applications of liquid chromatography. They are extremely permeable and offer a high efficiency that decreases slowly with increasing flow velocity.     

Monolithic column in gas chromatography

Monolithic columns invented in chromatographic praxis almost 40 years ago gained nowadays a lot of popularity in separations by liquid chromatographic technique. At the same time, application of monolithic columns in gas chromatography is less common and only a single review published by Svec et al. [1] covers this field of research. Since that time a lot of new findings on application and properties of monolithic columns in gas chromatography have been published in the literature deserving consideration and discussion. This review considers preparation of monolithic columns for GC, an impact of preparation conditions on column performance, optimization of separation conditions for GC analysis on monolithic columns and other important aspects of preparation and usage of monolithic capillary columns in GC. A final part of the review discusses the modern trends and possible applications in the future of capillary monolithic columns in GC.     

毛细管电色谱整体柱制备新进展

毛细管电色谱整体柱以其制备相对简单,渗透性好,柱效高等优点,成为电色谱领域中的一个研究热点.主要包括有机聚合物型和硅基整体柱两种类型.其制备方法还不十分成熟,有待完善.     

Monolithic Molecularly Imprinted Columns for Chromatographic Separation

Monolithic molecularly imprinted columns are a new class of column that emerged in the early 1990s. These monolithic materials are typically prepared directly inside stainless steel columns or capillary columns without the tedious procedures of grinding, sieving, and column packing. Furthermore, the preparation of this type of MIP is more cost-efficient, because the amount of template molecules required is much lower. In recent years monolithic supports as stationary phases in high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC) have attracted significant interest because of their ease of preparation, high reproducibility, versatile surface chemistry, and rapid mass transport.     

毛细管电色谱柱制备技术的进展

 介绍了毛细管电色谱开管柱、填充柱和整体柱的各种制备技术及其优势与不足,特别是对于近期发展的毛细管电色谱整体柱的制备方法及其应用进行了系统综述。引用文献100篇。     

功能化聚合物基质整体色谱柱的研究进展

新药研发、蛋白质组学分析、中药有效成分分析等前沿领域的快速发展,对分析检测方法的灵敏度、样品通量等提出了更高的要求,色谱分析技术面临着新的严峻挑战。作为色谱技术的核心,高效快速、高选择性的新型色谱柱开发是当前研究的热点之一。有机聚合物整体色谱柱因具有制备简单、通透性好、耐酸碱性能强、特别适合复杂样品的快速分离分析等优点,有望克服传统色谱分离介质的不足而备受关注。近年来分析对象越来越复杂,基于整体材料制备简单的特点,开发特殊功能化的新型整体色谱材料,成为分析科学及材料科学等领域研究者高度重视的方向之一。该文系统介绍了整体柱的发展史,并主要综述了有机聚合物整体色谱柱的发展及其功能化研究。     

整体柱离子色谱的研究进展

该文介绍了离子色谱的分类,整体柱的分类、制备及特点,并以此为依据归纳总结了常规整体柱在离子色谱中的应用和毛细管整体柱在毛细管离子色谱中的应用,其中包括硅胶基质整体柱和聚合物基质整体柱,评述并展望了整体柱离子色谱的发展前景.     

Properties of monolithic silica columns for HPLC.

Monolithic silica columns and their use in high peak-capacity HPLC separations are reviewed. Monolithic silica columns can potentially provide higher overall performance than particle-packed columns based on the variable external porosity and variable through-pore size/skeleton size ratios. The high permeability of monolithic silica columns resulting from the high porosity is shown to be advantageous to generate large numbers of theoretical plates with long capillary columns. High permeability together with the high stability of the network structures of silica allows their use in high-speed separations required for a second-dimension column in two dimensional HPLC. Disadvantages of monolithic silica columns are also described.     

Monolithic bed structure for capillary liquid chromatography

Monolithic stationary phases show promise for LC as a result of their good permeability, ease of preparation and broad selectivity. Inorganic silica monoliths have been extensively studied and applied for separation of small molecules. The presence of a large number of through pores and small skeletal structure allows the chromatographic efficiencies of silica monoliths to be comparable to columns packed with 5 μm silica particles, at much lower back pressure. In comparison, organic polymeric monoliths have been mostly used for separation of bio-molecules; however, recently, applications are expanding to small molecules as well. Organic monoliths with high surface areas and fused morphology rather than conventional globular morphology have shown good performance for small molecule separations. Factors such as domain size, through-pore size and mesopore size of the monolithic structures have been found to govern the efficiency of monolithic columns. The structure and performance of monolithic columns are reviewed in comparison to particle packed columns. Studying and characterizing the bed structures of organic monolithic columns can provide great insights into their performance, and aid in structure-directed synthesis of new and improved monoliths.     

Reduced-bore monolithic silica column modified with C8-TEOS for reversed-phase electrochromatography

Monolithic silica columns of 2.7 mm ID were prepared and derivatized with C8-TEOS and TEOS by on-column sol-gel reaction. These C8 large diameter monolithic silica columns gave 21 000 theoretical plates for aromatic hydrocarbons in 60% acetonitrile and 40% Tris-HCI buffer. The surface areas as well as the separation reproducibility were improved on coating by the sol-gel approach. Joule heating was greatly reduced by using monolithic columns to which fine quartz sand had been added during column preparation. Since this is a preliminary investigation on a monolithic column with such a large inner diameter, the separation efficiency was not so high as that presently achieved in normal capillary electrochromatography (CEC). However, use of the columns improved sample loadability and concentration detectability of electrochromatography, and semi-preparative separations could be performed.