多变量判别分析用于癌症诊断研究

用感应耦合等离子体原子发射光谱及石墨炉原子吸收光谱仪测定了正常人及癌症病人头发样品中15种元素的含量。所得数据用多元多项式扩展增维和逐步回归变量压缩技术以及PLS方法处理,得到了病人与正常人分类极为清晰的二维判别图。据此可将头发用作癌症临床诊断中的分析样品以取代血液样品。     

人工神经网络用于消化道癌症初级诊断研究

测定了正常人及消化道癌症病人头发样品１１种元素含量,然后将人工神经网络（ＡＮＮ）用于正常人与癌症患者的分类预测,并用独立预测样本作了检验,预报识别率达１００％。讨论了网络参数的选择和发样中微量元素与癌症的关系。结果表明该方法可作为消化道癌症初级诊断的一种辅助手段。     

再障头发、血清和骨髓组织钴、锂元素含量的变化

采用原子吸收光谱技术测定再障４３例患者头发、血清和骨髓组织的钴、锂元素含量，并与急性白血病２０例、缺铁性贫血１０例和正常人４４例作对照比较，再障头发、骨髓钴、锂和血清钴含量均低于正常人，Ｐ＜０．０５，而与急性白血病和缺铁性贫血之间，以及各种类型再障之间则无差异性．再障头发锂含量与血红蛋白、红细胞数呈负相关，Ｐ＜０．０５，说明再障确实存在着钴、锂元素代谢异常．     

用Delta—Bar—Delta规则神经网络对肺癌患者的判别

采用Ｄｅｌｔａ规则和Ｄｅｌｔａ－Ｂａｒ－Ｄｅｌｔａ规则的神经网络，用于根据头发和血清样品中金属含量对正常人与癌症患者的分类判别研究，对有关参数影响作了讨论，结果表明，采用Ｄｅｌｔａ－Ｂａｒ－Ｄｅｌｔａ规则的神经网络训练速度明显快于Ｄｅｌｔａ规则的神经网络。     

头发检测与疑难病诊断

综述了我国用头发诊断疑难病,包括恶性肿瘤、心脑血管疾病、尘肺的研究概况。20余年来,中国科技人员创造了4类利用头发微量元素谱诊断疾病的方法。18项研究报告表明,头发微量元素谱可将癌症病人与健康人正确区分开来,为临床癌症诊断奠定了坚实的实验基础,也为利用头发样本代替血液样本作人群癌症筛查提供了理论依据;11项研究结果表明,借助头发微量元素谱,可相当准确地判别待测样本的健康属性,可以诊断和预报疾病,可以筛选高危人群,对心脑血管病的早发现、早诊断、早治疗具有重要的潜在应用价值;9项研究结果证明,测量头发元素,建立判别方法,可以从待检样品准确诊断尘肺患者和找出尘肺高危人群,并可提早3～5年对尘肺的发生作出预报。实践表明,头发检测是疑难病筛查的一种简便、经济、实用、有效的新方法。     

用多变量统计分析方法辨识肺癌患者分类中的关键元素(英文)

用逐步判别、主成分分析和聚类方法研究了根据血清和毛发样品中元素含量对正常人和肺癌患者分类中的关键元素．用主成分分析的结果表明,在肺癌患者与正常人的分类中,血清中的Ｃａ,Ｃｒ,Ｃｕ,Ｐ和Ｚｎ是关键元素,而毛发中的Ａｌ,Ｂ,Ｃｒ,Ｐ和Ｓｒ是关键元素．对于正常人和癌症患者元素之间的欧氏距离不同     

人工神经网络方法用于肺癌的辅助诊断

根据人发和血清中微量元素的含量，将人工神经网络用于正常人与癌症患者的分类预测。用独立预测样本作了检验，表明该方法可作为肺癌诊断的一种辅助手段。讨论了当预测样本中有元素缺损时ＡＮＮ的分类预测情况，并研究了人发和血清样品中的元素对分类的影响。     

原子吸收光谱法测定原发性癫痫患者头发中微量元素

通过原子吸收光谱法测定原发性癫痫患者头发中的微量元素Ｚｎ，Ｃｕ，Ｆｅ，Ｍｎ，Ｐｂ和宏量元素Ｋ，Ｎａ，Ｃａ，Ｍｇ并与正常人头发中的微量元素比较，结果表明，：癫痫患者头发中的锰含量明显高于正常人，这一结论与有关文献的结论吻合。     

火焰原子吸收光谱法测定头发和指甲中金属元素

人体的健康与营养均与微量元素密切相关,而头发和指甲又是最易得到并在一定程度上代表人体组织的特征样品。为此,本文研究了用逆王水(硝酸和盐酸为3:1)和高压密封罐溶解样品,以火焰原子吸收光谱法测定头发和指甲中的钾、钠、钙、镁、铁、铜、锌和锰等元素,并讨论了共存元素和酸度对测定的干扰及消除。试验表明,本法简便可靠,回收率在93％～106％范围,RSD小于5％。     

表面活性剂稀释火焰原子吸收法测定钙镁铜锌铁

用中性表面活性剂聚乙二醇辛基苯基醚稀释样品，火焰原子吸收法测定血清中钙、镁、铜、锌铁及尿样中钙、镁、消除了样品基体干扰，提高了钙、镁测定灵敏度，操作简便，并讨论了表面活性消除干扰的作用机理。     

Diagnosis of lung cancer based on metal contents in serum and hair using multivariate statistical methods

The classification of normal and cancer groups with four multivariate methods according to metal contents in serum and hair samples has been discussed in the present paper. Results show that the four multivariate methods, stepwise discrimination analysis, principal components analysis, hierarchical cluster analysis, and stepwise cluster analysis can distinguish the two groups correctly. The independent samples of both normal and cancer groups were tested and can be distinguished correctly by the four methods. Therefore, these methods can be used as an aid for diagnosis of lung cancer according to the metal contents in serum and hair samples.     

Determination of Zn/Cu ratio and oligoelements in serum samples by total reflection X-ray fluorescence spectrometry for cancer diagnosis

The method of quantification using the Compton peak as an internal standard, developed in a previous work, was applied to the routine determination of Fe, Cu, Zn and Se in serum samples from normal individuals and cancer patients by total reflection X-ray fluorescence spectrometry. Samples were classified according to age and sex of the donor, in order to determine reference values for normal individuals. Results indicate that the Zn/Cu ratio and the Cu concentration could prove to be useful tools for cancer diagnosis. Significant differences in these parameters between the normal and cancer group were found for all age ranges. The multielemental character of the technique, coupled with the small amounts of sample required and the short analysis time make it a valuable tool in clinical analysis.     

微量元素砷与胃癌关系的研究

从福建省胃癌高(长乐市)、低发区(福安市)的土壤、水体及人体血清、头发、尿液采集的材料入手,分析了砷元素与恶性肿瘤关系。结果表明,胃癌低发区土壤、饮用水和人头发砷含量反比高发区高。可见在正常情况下砷不致癌,适量砷可抑制癌症。     

A multiplexed bead assay for profiling glycosylation patterns on serum protein biomarkers of pancreatic cancer

A multiplexed bead-based immunoassay was developed to simultaneously profile glycosylation patterns of serum proteins to investigate their usefulness as biomarkers for pancreatic cancer. The multiplex assay utilized protein-specific capture antibodies chemically coupled individually to beads labeled with specific amounts of fluorescent dye. Captured proteins were detected based on the extent and specific type of glycosylation as determined by successive binding of fluorescent lectin probes. Advantages to this technique include the fact that antibodies coupled to the beads had minimal nonspecific binding to the lectins ConA/SNA, avoiding the step of chemically blocking the antibody glycans and the bead assays were performed in a 96-well filter plate enabling high-throughput screening applications with improved reproducibility. The assay was tested with ConA and SNA lectins to examine the glycosylation patterns of α-1-β glycoprotein (A1BG) and serum amyloid p (SAP) component for use as potential biomarkers for the detection of pancreatic cancer based on the results from prior biomarker studies. The results showed that the SNA response on the captured A1BG protein could distinguish chronic pancreatitis samples from pancreatic cancer with a p-value of 0.035 and for the SAP protein with SNA, a p-value of 0.026 was found between the signal of normal controls and the pancreatic cancer samples. For the ConA response, a decline in the signal for both proteins in the serum samples was found to distinguish pancreatic cancer from normal controls and renal cell carnoma samples (A1BG, p<0.05; and SAP, p<0.0001).     

Study of normal and modified nucleosides in serum by RP-HPLC

Summary Modified nucleosides derived predominantly from transfer ribonucleic acid (tRNA) have been studied as possible tumor markers. In this paper a reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been applied to study 15 normal and modified nucleosides in serum. The nucleoside levels in normal human serum were established, and the concentrations of 15 nucleosides in serum from 19 cancer patients were determined. It was found that the concentrations of modified nucleosides were significantly higher in patient sera. Based on 15 nucleoside concentrations in serum, factor analysis could classify correctly 90% of cancer patients from the normal humans. Further work showed that urine was slightly better than serum when determining nucleosides as biological marker candidates. More work is ongoing to determine the clinical usefulness of modified nucleosides as tumor markers.     

胃癌患者与非癌对照血清微量元素含量对比研究

在医院选择３３例经病理学诊断的胃癌患者及７９例非癌对照，测定其血清锌、铜、锰、钼、硒含量并进行对比分析。结果显示，胃癌患者血清铜含量显著低于对照组（Ｐ＜０．０５））；血清硒含量亦显著低于对照组（Ｐ＜０．００２）．曾报道，江西土壤有效钼含量与胃癌死亡率呈负相关．江西胃癌患者发钼含量显著低于非癌对照．硒是抗氧化剂和抗氧化酶的重要成分，在抑制自由基反应、清除过氧化物方面起重要作用．显然钼、硒与胃癌关系十分密切。缺乏钼、硒是造成胃癌发病率升高的危险因素。