基于抗菌肽的生物传感器在食源性致病菌检测中的应用

快速检测食源性致病菌是预防食源性疾病大量暴发的必要措施.基于生物传感器的食源性病原菌检测技术具有灵敏度高、实时定量、操作简便等优点.抗菌肽作为识别分子具有稳定性高和成本低的特点,在食源性致病菌的快速检测中得到了广泛的应用.将抗菌肽与生物传感器结合用于食源性致病菌具有潜在的实际应用价值.本文综述了基于抗菌肽的电化学方法和...     

基于金属有机骨架材料的电化学生物传感器在癌症检测中的应用

金属有机骨架(metal-organic frameworks, MOFs)材料因具有稳定的骨架结构、可调的孔尺度和大的比表面积等优良特性,被作为固定生物探针的基体,用于构建电化学生物传感器.电化学生物传感器是一种以电极为信号转换器,通过敏感元件,将肿瘤标志物的高特异性与电化学传感器的高灵敏度相结合的检测装置,在临床癌症筛查方面具有重大应用.本文概述了金属有机骨架复合材料的分类,总结了过去五年基于有机金属骨架材料的电化学生物传感器在检测作为癌症早期诊断指标的各种标志物(如癌症标志物、microRNA和DNA)方面的进展,并对其未来发展进行了展望.     

金属有机框架传感器在农药检测中的应用研究进展

农药在农业生产中占据重要地位,但不合理使用会对环境和人类的健康造成不利的影响。因此,开发快速、灵敏和选择性高的农药检测技术引起了广泛关注。本文总结了金属有机框架（MOF）传感器的构建及其在农药检测领域应用的最新进展,深入阐述其传感机理。酶、分子印迹聚合物、染料/量子点、适配体被用于MOF传感器的构建,以提高其选择性和灵敏度。MOF光学传感器已被用于农药的快速检测和现场诊断。最后,总结和讨论了MOF传感器在农药检测领域中存在的问题和面临的挑战。     

金属有机骨架材料在传感器中的应用

摘 要 金属有机骨架（Metal Organic Framework,MOFs）是由有机配体与金属离子或金属离子簇通过配位作用自组装而成的一种具有永久孔道性的开放结晶骨架,通常也被称为配位聚合物（PCPS）。因为其较大的比表面积、规整的孔道结构、热稳定性和化学可裁剪性,使其在多个领域具有广阔的应用前景。近年来,随着MOFs在传感领域的发展,许多不同的功能基团被引入到MOFs的孔道中,研制出具有荧光识别性能的MOFs。本论文综述了近几年来基于MOFs的化学传感器在离子识别、PH检测、挥发性有机物和气体检测、爆炸物识别和生物分子检测等关键领域的研究进展,并对MOFs在化学传感器的应用前景进行了展望。     

基于金属有机框架材料的光/电化学传感器在水环境检测中的应用进展

随着人们对水环境质量的要求日益增加,开发简便、灵敏和准确的新型水环境检测技术已成为研究的热点.金属有机框架化合物(Metal-organic frameworks,MOFs)是一类由金属离子或团簇和有机配体自组装形成的多孔配位聚合物,因其具有吸附可逆、催化活性高、比表面积大、孔径可调和结构多样等特性,可作为光/电化学传...     

金属有机骨架复合材料生物传感器检测肿瘤标志物的应用进展

生物传感器是传统肿瘤标志物检测方法的有效替代方法,而基于金属有机骨架(MOF)复合材料的生物传感器在肿瘤标志物检测方面具有速度快、灵敏度高、检出限低、成本低等优点.综述了MOF及其复合材料的分类,介绍了基于MOF复合材料的生物传感器在检测肿瘤标志物方面的应用进展,并对基于MOF复合材料的生物传感器未来发展趋势进行了展望...     

万古霉素在食源性致病菌检测中的应用

食源性致病菌污染是引起食品安全的主要因素之一,对人类健康构成了严重威胁。万古霉素作为一种对革兰氏阳性菌具有特异性识别功能的小分子,能够修饰在磁性纳米粒子表面,使粒子获得靶向目标菌的能力,结合其他检测方法,可以实现分离检测革兰氏阳性菌的目的。本文对万古霉素功能化的磁珠在革兰氏阳性菌分离检测的研究进展进行了介绍,分析了万古霉素不同修饰方式和检测方法对其检测效果的影响,并对各种方法的检测结果进行了分析比较,最后,提出了万古霉素在食源性致病菌检测中存在的问题,并对其未来应用进行了展望。     

基于金属有机骨架材料的电化学DNA传感器在分析检测领域的应用进展北大核心CSCD

电化学DNA传感器是基于DNA探针与目标DNA之间碱基互补配对原则构建的传感器,根据识别元件与目标物结合前后信号变化实现目标物的检测,已成为传统检测方法的有效替代方法。而金属有机骨架材料(MOFs)具有比表面积大、孔隙率高、孔径可调和热稳定性强等诸多优点,引起学者的广泛关注,已初步用于电化学DNA传感器的构建,并用于肿瘤标志物、抗生素及重金属等的灵敏、准确检测。为此,综述了电化学DNA传感器的DNA探针固定方法及信号物质,重点介绍了基于MOFs的电化学DNA传感器在分析检测领域的应用进展,并对其未来发展方向进行了展望(引用文献50篇)。     

Array biosensor for detection of toxins

The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and competitive fluoroimmunoassays have been developed to detect high and low molecular weight toxins, respectively, in complex samples. Recognition molecules (usually antibodies) were first immobilized in specific locations on the waveguide and the resultant patterned array was used to interrogate up to 12 different samples for the presence of multiple different analytes. Upon binding of a fluorescent analyte or fluorescent immunocomplex, the pattern of fluorescent spots was detected using a CCD camera. Automated image analysis was used to determine a mean fluorescence value for each assay spot and to subtract the local background signal. The location of the spot and its mean fluorescence value were used to determine the toxin identity and concentration. Toxins were measured in clinical fluids, environmental samples and foods, with minimal sample preparation. Results are shown for rapid analyses of staphylococcal enterotoxin B, ricin, cholera toxin, botulinum toxoids, trinitrotoluene, and the mycotoxin fumonisin. Toxins were detected at levels as low as 0.5 ng mL^–1.     

A highly stable terbium metal-organic framework for efficient detection of picric acid in water

A highly stable fluorescent terbium MOF (Tb₄(paip)₆·1.2H₂O, paip = 5-(1H-pyrazole-4-yl)isophthalate) showing a sharp green emission (545 nm) and a quantum yield of 21.0% was successfully synthesized. This compound is shown to be a recyclable sensor for detecting picric acid in aqueous solution with both high sensitivity and selectivity, attributed to the electron transfer quenching mechanism.     

Dielectrophoresis assisted immuno-capture and detection of foodborne pathogenic bacteria in biochips

This study integrated dielectrophoresis (DEP) with non-flow through biochips to enhance the immuno-capture and detection of foodborne pathogenic bacteria. It demonstrated two major functions provided by DEP to improve the chip performance: (i) concentrating bacterial cells from the suspension to different locations on the chip surface by positive and negative DEP; (ii) making the cells in close contact with the immobilized antibodies on the chip surface so that immuno-capture efficiency can be dramatically enhanced.The microchip achieved the immuno-capture efficiencies of ∼56.0% and ∼64.0% to Salmonella cells with 15 and 30 min DEP, respectively, which were considerably higher than those of ∼10.4% and ∼17.6% for 15 and 30 min immuno-capture without DEP. The immuno-captured bacterial cells were detected by the sandwich format ELISA on the chips. The final absorbance signals were enhanced by DEP assisted immuno-capture by 64.7-105.2% for the samples containing 10³-10⁶ cells/20 μl. The integration of DEP with the biochips has the potential to advance the chip-based immunoassay methods for microbial detection.     

The application of metal-organic frameworks in electrocatalytic nitrogen reduction

In recent years,the research of nitrogen reduction reaction(NRR) under ambient conditions has attracted wide attention for their relatively low energy consumption,in which rational design of electrocatalysts is the key to achieve high-performance NRR.Metal-organic frameworks(MOFs),as a new kind of porous material,have been intensively studied in the past few decades owing to not only their structural versatility and tunability but also intrinsic porosity.Due to their structural features,MOFs als...     

金属-有机框架材料在催化炔烃半氢化反应中的应用

炔烃的半氢化反应在有机合成和精细化工领域具有重要地位,如何同时兼顾反应活性和选择性仍存在很大挑战。目前已有多种材料被应用于相关催化,其中金属-有机骨架(MOFs)及其复合材料受到越来越多关注。MOFs的多孔性、结构可修饰性、空间限域效应、协同催化等优点,使其在炔烃的半氢化反应中表现出独特的应用前景。本文综述了MOFs及其复合材料在炔烃的半氢化反应生成烯烃过程中的应用,主要根据活性催化位点的类别展开介绍,重点阐述了不同体系中催化效果和结构之间的关系。     

Conformational analysis of 1,2-dichloroethane adsorbed in metal-organic frameworks

This paper describes the conformational analysis of 1,2-dichloroethane adsorbed into three different metal-organic frameworks, MIL-53(Al), MIL-68(In), MIL-53-NH₂(Al), by using FT-Raman spectroscopy in combination with powder XRD and TGA. For non-polar frameworks, the main guest-host interactions are van der Waal interactions between the CH bonds of 1,2-dichloroethane (DCE) and the π system of terephthalate ligands. The polar framework of MIL-53-NH₂ is able to stabilize the gauche conformation of DCE at room temperature. The conformational enthalpy of each system was determined through variable temperature FT-Raman spectroscopy. Furthermore, the line-width of the Raman bands provides information regarding the molecular motion of the halocarbons at various temperatures inside the framework.     

A novel type of matrix for surface-assisted laser desorption–ionization mass spectrometric detection of biomolecules using metal-organic frameworks

A 3D metal-organic framework (MOF) nanomaterial as matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and tandem mass spectrometry (MS/MS) was developed for the analysis of complex biomolecules. Unlike other nanoparticle matrices, this MOF nanomaterial does not need chemical modification prior to use. An exceptional signal reproducibility as well as very low background interferences in analyzing mono-/di-saccharides, peptides and complex starch digests demonstrate its high potential for biomolecule assays, especially for small molecules.     

Tuning the release rate of volatile molecules by pore surface engineering in metal-organic frameworks

Encapsulation and controlled release of volatile molecules such as fragrances in a designed manner is important but challenging for the flavor and fragrance industry. Here, we report the tuning release of volatile molecules by postsynthetic modification of an amine-terminated metal-organic framework(MOF) MIL-101-NH_2. By amidation, we obtained three MIL-101 MOFs, the trimethylacetamideterminated TC-MIL-101, the benzamide-terminated BC-MIL-101, and the oxalic acid monoamideterminated OC-MIL-101. All the MOFs can efficiently encapsulate volatile molecules. Moreover, we demonstrate that the release profile of volatiles can be widely tuned to sustain the release in several days to months and even over a year using different modified MIL-101 MOFs. We show that the release profiles are correlated with the binding energies between the guest volatiles and pores in MOFs. The pore diffusion and the synergistic transport are the rate-limiting step of the guest molecules from the modified MOFs.     

基于G-四链体的生物传感器在食品污染物检测中的研究进展

食品污染物不仅对人类健康造成了严重威胁,还会给食品工业造成巨大的经济损失。G-四链体(G4)是由鸟嘌呤的碱基配对形成的核酸三维二级结构,具有灵活的绑定能力,已成为生物传感器的重要组成部分。将G4与生物传感器结合用于食品中污染物的检测得到了广泛的应用。本文对G4进行了简介,综述了2015～2022年间G4在食品污染物检测中的研究进展,并对其未来的发展趋势进行了展望。