基于智能手机现场快速检测技术研究进展

作为一种便携式的功能性移动电子设备,智能手机的广泛应用为便携式检测提供了发展平台,并在临床诊断、食品安全分析和环境监测等领域表现出巨大的应用潜力。本文综述了2018～2022年,基于智能手机与比色法、荧光法、化学发光法、电化学分析、免疫分析及微流控分析等技术联用的即时检测应用研究进展,并对其发展趋势和应用前景进行了展望。     

流动分析-红外光谱联用技术在农药和食品分析中的应用

综述了流动分析-红外光谱联用技术在农药残留、农药制剂质量控制、食品检测(包括乙醇、糖类、有机酸和咖啡因)等领域的应用,并对该技术的应用前景进行了展望(引用文献52篇)。     

智能手机应用于便携式检测技术的研究进展

智能手机在近十年间得到了迅速发展,其功能日渐强大。作为一种简便易携的功能性移动设备,智能手机的普及为便携式检测提供了发展机遇。智能手机具备轻巧性、便携性、强大的应用程序处理功能等特点。其操作简单,只需对待测物质进行图片采集或者数字化处理即可完成检测,操作者不需专门培训就能掌握。因此,该技术成为近年来分析检测领域的研究热点。本文综述了智能手机应用于便携式检测技术的研究现状,并对其今后的发展趋势和应用前景进行了展望,引用文献49篇。     

质谱在高通量快速检测技术中的应用研究进展

质谱是现代分析测试技术中同时具备了灵敏度高、特异性好、分析速度快等特性的普适性方法,现已被广泛应用于食品检验、环境监测、药物筛选、临床医学等众多领域的高通量快速检测技术中。该文针对近年来低分辨质谱、高分辨质谱以及原位电离质谱技术在高通量快速检测,特别是小分子化合物的高通量快速检测中的应用研究进展进行了综述,并对质谱技术用于高通量快速检测的发展趋势及应用前景进行了展望,以期为相关领域的科技人员提供理论支持和技术参考。     

适配体传感器在农药残留检测中的应用

基于脱氧核糖核酸的生物检测技术在食品中有毒有害物质检测方面发挥着日益重要的作用。其中,作为一类具有良好应用前景的分析工具,以适配体技术为基础的快速检测方法与传统检测方法相比,具有简单便携、响应迅速、灵敏度高、特异性好、成本低等显著优势,因此在近些年成为分析检测领域研究的热点之一。该文总结了近几年应用于食品安全特别是农药残留检测的几种适配体检测技术(包括比色法、荧光法以及电化学法),并对其检测特点及应用进行了介绍。     

薄层色谱分析法及其进展

介绍常规薄层色谱和几种高效薄层色谱分析方法,薄层色谱与红外光谱、表面增强拉曼光谱、核磁共振、质谱、电化学等联用检测技术,以及薄层色谱与联用检测技术在医药、生物制品、毒物、环境有害物质、食品及其他领域定性定量分析中的应用,并对其前景进行了展望。     

化学发光免疫分析方法的应用研究进展

化学发光免疫分析方法因其灵敏度高、特异性好、分析速度快、操作简便等优势,在临床诊断、食品检测、环境监测等领域得到了广泛应用.建立高灵敏、高特异、高通量的化学发光免疫分析方法成为近年来的研究热点和发展趋势.本文对化学发光免疫分析自2011年以来新方法及联用技术进行了综述,并对其在临床诊断及食品安全等领域的应用进行了介绍,最后对该领域的研究前景进行了展望.     

成像技术在食品安全与质量控制中的研究进展

食品质量与安全是政府、食品行业以及消费者十分关注的问题。为了保证食品质量与安全，需要对食品中的风险因子进行检测。传统的分析方法如生物化学方法和仪器分析方法（色谱法、色谱-质谱法）存在前处理比较复杂，耗时，对样品具有破坏性及无法获取目标物空间信息等缺点。因此，开发快速，无损，实时和可视化的检测技术十分重要，这也是食品领域研究的热点。近年来，高光谱成像技术融合了成像和光谱两种技术，可以作为一种用于食品质量和安全评估的非破坏性和实时检测的工具。拉曼光谱成像技术可以同时获得待测物的光谱和空间信息，具有快速，无损和低成本等优点，在食品安全评价和质量控制中也得到了成功应用。质谱成像技术不需要标记和染色，即可实现样品组织表面待测物的可视化和高通量分析。它作为一种分子可视化技术，可以获得食品中营养成分及内、外源性有害物质的空间分布信息，在食品领域也表现出良好的应用前景。本文检索了近几年国内外发表的成像技术在食品研究中的相关文献，介绍了高光谱成像技术、拉曼光谱成像技术和质谱成像技术的原理，并综述了它们在食品安全与质量控制中的应用。此外，本文分析和讨论了这几种成像技术的优缺点，并对成像技术在食品领域的发展前景做出了展望。     

核酸介导信号放大光学生物传感器在食品污染物检测中的应用EI北大核心CSCD

信号放大是新型生物传感分析过程中重要的环节。核酸介导的信号放大技术凭借核酸材料灵活的结构设计、低成本和易于制备等特点,在生物传感快速检测技术的开发上逐渐发展成为一项重要的分支,广泛应用于食品、环境和医药等新型检测方法开发。介绍了传统和新型核酸扩增技术、生物条形码和DNA walker等信号放大机理和应用,同时进一步综述核酸信号放大技术结合光学生物传感在食品污染物中检测的应用,如化学污染物、毒素类污染物、和重金属污染物等,并对核酸介导的信号放大技术在食品污染物检测中的问题和前景进行讨论。     

塑料食品接触材料样品前处理与检测技术研究进展

塑料食品接触材料是食品接触材料类别中最常见的一种材料,其本身可能含有的潜在化学危害物质在与食品接触过程中发生吸收、溶解、扩散等迁移行为,进而造成食品污染,危害食品质量安全。采用适宜的样品前处理和检测技术对迁移物进行鉴别分析是塑料食品接触材料安全所面临的巨大挑战。该文就塑料食品接触材料的样品前处理技术和仪器分析技术进行了综述,并展望了其发展趋势和应用前景,旨在为从事塑料食品接触材料质量安全研究和检测的技术人员提供技术参考。     

数字PCR在食品安全检测中的应用研究进展

聚合酶链反应(PCR)在动植物源掺杂鉴别、转基因成分和致病微生物等食品安全检测领域成为日趋重要的检测技术。从传统PCR、荧光PCR到数字PCR,PCR技术逐渐从定性分析、半定量分析发展到准确定量,不仅提升了准确度和检测效率,同时也扩展了食品检测范围,使食品安全的监管更加精细化。该文总结了近5年来数字PCR在食品安全检测中的研究进展,比较了传统PCR、荧光定量PCR和数字PCR的相关标准制订情况,列举、讨论了数字PCR在不同食品安全领域检测中的技术进展和存在的问题,并对数字PCR未来发展方向进行了展望。     

Smartphone-based colorimetric determination of glucose in food samples based on the intrinsic peroxidase-like activity of nitrogen-doped carbon dots obtained from locusts

In this study, nitrogen-doped carbon dots (N-CDs) with excellent peroxidase-like activity were prepared using locust powder as the carbon source by a self-exothermic reaction. The obtained N-CDs could catalyze the oxidation of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate a blue oxidized product (TMB_ox) with a maximum absorption peak at 654 nm. The catalytic reaction conditions were optimized; furthermore, steady-state kinetic analysis indicated that N-CDs exhibited high affinity toward both TMB and H₂O₂, and the Michaelis-Menten constant (k_m) values were 0.115 mM (TMB) and 0.764 mM (H₂O₂). A smartphone-based colorimetric method was developed for quantitative detection. The 1/L values (L stands for lightness in HSL color space) of the TMB_ox solution were recorded via an iPhone application Color Analyzer. Since H₂O₂ is the by-product of glucose (Glu) oxidation in the presence of glucose oxidase (GOx), a simple, sensitive, and selective smartphone-based colorimetric method was developed for the determination of Glu, and the detection limit was 1.09 μM. The smartphone-based method was successfully applied to determine Glu in different food samples with recoveries in the range of 88.5–109.0 %.     

薄膜基荧光传感检测的研究进展

薄膜基荧光传感器是继离子迁移谱之后,业界公认的一种最具发展潜力的微痕量物质探测技术.由于其具有灵敏性、便携性、实时检测、响应速度快、易于制造、不污染待测体系等优点,在食品检测、环境监测、质量控制和生物医学分析等领域引起了广泛的关注和研究.本文主要综述了近年来薄膜基荧光传感在挥发性气体检测、有毒化学品检测、爆炸物检测、溶液相离子检测以及生物监测等领域的研究进展,并提出了薄膜基荧光传感所面临的挑战与未来的发展方向.     

实时直接分析质谱新技术及其应用

由于不需要复杂的样品前处理过程,并具有实时、原位分析等特点,常压敞开式离子化技术成为近年来质谱研究的热点之一.实时直接分析(direct analysis in real time,DART)是近年来出现的一种常压敞开式离子化新技术,在食品安全、环境监测、爆炸物检测以及生物医药等诸多研究领域均有广泛的应用.本文简单介绍了常压敞开式离子化方法的发展,DART的基本原理和研究现状.进而介绍了我国质谱研究人员在基于DART的质谱仪器改进、联用技术以及生物药物等检测分析方面取得的新进展和新成果.     

Upsurgence of smartphone as an economical,portable, and consumer-friendly analytical device/interface platform for digital sensing of hazardous environmental ions

Ions play a pivotal role in the biological regulatory processes and catalyzing enzymatic reactions; however, increased levels in the human body leads to many health risks or toxicity. To circumvent this, periodic and precise monitoring of significant ions in environmental, biological, chemical, and food samples are necessary, which need to be mapped/monitored continuously. This has prompted researchers to develop cost-effective, handy, and rapid techniques which can be fruitful for even untrained personnel by obviating manual user instructions, lengthy sample preparation steps, and costly instruments. The exploitation of user-friendly behavior, affordable price, and ubiquitous usage of smartphones has led to the development of a plethora of smartphone-based methodologies whereby they can serve as devices, detectors, or interfaces. Their in-built high-resolution rear camera, ambient light sensors, wireless connectivity, internal storage, and global positioning systems minimize the cost and simplify the fabrication of developing point-of-care testing devices, making them operable in challenging conditions with limited resources. Coupling smartphones with iCloud technology allows the synchronous storing and online transmitting of databases to consumers even in remote areas, which helps in real-time monitoring and continuously scrutinizing contaminants in the environment. This is not an exhaustive review but enumerates the progress made in the development of smartphone-based analytical aids by incorporating advanced device fabrication strategies and hassle-free analytical protocols during the past years (2014–2021). An account of key features like sensing performance of the developed methods in terms of selectivity, sensitivity, and detection limits and their limitations for recognition of environmental and biologically eminent ions is also discussed. Lastly, this review paves the way for the development of advanced innovative analytical techniques employing smartphone technology for the foreseeable future to ensure point-of-care human safety.     

Quenching Behavior of the Electrochemiluminescence of Ru(bpy)32+/TPrA System by Phenols on a Smartphone-Based Sensor

Phenolic compounds such as vanillic and p-coumaric acids are pollutants of major concern in the agro-industrial processing, thereby their effective detection in the industrial environment is essential to reduce exposure. Herein, we present the quenching effect of these compounds on the electrochemiluminescence (ECL) of the Ru(bpy)₃²⁺/TPrA (TPrA=tri-n-propylamine) system at a disposable screen-printed carbon electrode. Transient ECL profiles are obtained from multiple video frames following 1.2 V application by a smartphone-based ECL sensor. A wide range of detection was achieved using the sensor with limit of detection of 0.26 μM and 0.68 μM for vanillic and p-coumaric acids, respectively. The estimated quenching constants determined that the quenching efficiency of vanillic acid is at least two-fold that of p-coumaric acid under the current detection conditions. The present ECL quenching approach provided an effective method to detect phenolic compounds using a low-cost, portable smartphone-based ECL sensor.     

高效液相色谱法测定氨基酸的研究进展

氨基酸是构成生物体的基础物质,氨基酸分析是生命科学研究中最重要的领域之一。高效液相色谱法因分析速度快、操作简便、检测灵敏、适用范围广等优点而广泛应用于食品工业、制药工业、生命科学研究等领域。该文综述了高效液相色谱分析氨基酸的方法,包括柱后衍生法、柱前衍生法、高效阴离子交换色谱-积分脉冲安培检测法和高效液相色谱-蒸发光散射检测法。并对上述方法进行了比较,为日常的氨基酸分析提供了参考。     

The smartphone biosensors for point-of-care detection of human infectious diseases: Overview and perspectives—A systematic review

The accurate and fast diagnosis of infectious diseases plays a critical role in the timely and tailored treatment of the patients and preventing the infections from the further spread. This is especially necessary in regions with low- and middle-income of the world. According to the smartphone-based sensory applications such as the inimitable ability to obtain portable, user-friendly, accurate, and effective functions, that in turn, converts lab-on-hardware an interesting area of new investigations. In the present review, original research articles published in English were collected from various databases during January 2011–July 2021. Also, the reference lists of the articles have been searched. In between 121 electronically searched citations, 35 articles had the inclusion criteria. The maximum potential and extended utilization of smartphone-based biosensors in the diagnosis of human infectious agents belonged to colorimetric smartphone biosensors. Also, because of their unique futures in easy visualization, comfortable analyzing and processing by smartphone, they are easier to use for point-of-care products. Based on the results, smartphone-based biosensors are rapid, precise, and low-cost diagnostic methods and increasing their quality in limited-resource settings is of major importance. In addition, their abilities to connect to the “cloud” data storage systems and the Internet of Things (IoT) networks for collecting and sharing medical analytical data provide a trending novel research field in medical information technology.     

鹅膏毒肽检测方法的研究进展

一直以来毒蘑菇中毒在全球范围内频发,成为最常见的食物中毒事件之一,也是食物中毒致死的最主要原因。毒蘑菇中含量最高、致死性最强的毒素为鹅膏毒肽。因此,鹅膏毒素准确快捷的检测对鉴别中毒源、患者后续治疗及预防有着重要意义。目前,鹅膏毒肽的常用检测方法包括色谱-质谱联用技术以及一些基于免疫识别和特殊化学结构的检测技术。而不断开发特异性强、灵敏度高、检测时间短的新型检测方法仍是目前的研究热点和重点。该文就鹅膏毒肽的检测方法进行综述,以期引起研究同行对此领域的关注,以激发更多的研究思路。     

Recent progress on microfluidic biosensors for rapid detection of pathogenic bacteria

Rapid on-site detection of pathogenic bacteria with high sensitivity and specificity is becoming an urgent need in public health assurance, medical diagnostics, environmental monitoring, and food safety fields. Despite being reliable and widely used, the existing methods of bacteria detection are cumbersome and time-consuming, which is not conducive to field detection. Microfluidic lab-on-a-chip technology has provided a detective tool for various analytes, due to its miniaturization, portabilit...