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采用荧光光谱和三维荧光光谱法研究了橙皮素(HSP)与牛血清白蛋白(BSA)之间的相互作用,并考察了共存金属离子Cu2+,Zn2+对二者相互作用的影响。实验结果表明,HSP对BSA的内源性荧光具有猝灭作用,猝灭类型为静态猝灭,作用力类型是氢键和范德华力,Cu2+,Zn2+的加入未改变HSP对BSA的猝灭类型和作用力类型。通过比较猝灭常数、结合常数、结合位点数、猝灭效率和三维荧光光谱图变化,推知Cu2+,Zn2+能与BSA产生结合作用,使其成为受制状态下的刚性肽链,从而影响HSP进入BSA疏水腔,减弱了HSP与BSA的结合能力,表现为与HSP存在竞争作用。 相似文献
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采用荧光光谱、同步荧光光谱、紫外-可见吸收光谱及分子模拟技术研究了模拟生理条件下丽春红2R(P2R)与牛血清白蛋白(BSA)的相互作用。实验结果表明,P2R-BSA体系的荧光猝灭机制为内源荧光猝灭,猝灭原因为静态猝灭和非辐射能量转移;计算了不同温度下体系的结合常数Ka及结合位点数n;根据热力学参数推断出作用力类型;求出室温下荧光给体-受体间的结合距离;同步荧光法证实丽春红2R对BSA构象未产生影响;分子模拟研究结果表明二者间的主要作用力为氢键和疏水作用力。 相似文献
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采用荧光光谱、紫外-可见光谱技术研究了芦丁与牛血清白蛋白(BSA)在金属离子Cu2+/CO2+共存时的相互作用.研究结果表明:有或无金属离子存在时,芦丁对BSA的荧光猝灭作用都是由于生成复合物而引起的静态猝灭;金属离子的存在使芦丁与BSA的表观结合常数KLB增大,且Co使KL)增大得更明显.由热力学参数确定芦丁和BSA... 相似文献
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应用荧光光谱、紫外-可见分光光度法研究了盐酸鸟嘌呤(GH)与牛血清白蛋白(BSA)的相互作用。结果表明:GH能猝灭BSA的荧光强度,其猝灭机理为静态猝灭。采用位点结合模型公式和热力学公式计算了结合常数、结合位点数及结合类型。用同步荧光技术研究GH对BSA构象的影响。 相似文献
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维生素B1与牛血清白蛋白的相互作用 总被引:1,自引:0,他引:1
采用荧光猝灭光谱、同步荧光光谱研究了维生素B1与牛血清白蛋白(bovine serum albumin,BSA)的相互作用机制.维生素B1对BSA 有较强的荧光猝灭作用,结合Stern-Volmer方程和热力学方程得到结合常数和对应温度下的热力学参数,并结合紫外吸收光谱进一步验证,维生素B1对BSA的猝灭过程为动态猝灭,二者主要以氢键和范德华力结合.并采用同步荧光光谱探讨了维生素B1对BSA 构象的影响.此外,讨论了共存Ca2+、Cu2+、Al3+、Mg2+ 和K+ 对维生素B1与BSA结合作用的影响. 相似文献
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运用荧光光谱、吸收光谱研究了灯盏花素(Breviscapinun,BR)与牛血清白蛋白(Bovine Serum Albumin,BSA)的相互作用.BR对BSA的荧光光谱具有猝灭作用,其猝灭机制为静态-动态联合猝灭,BSA发射峰蓝移.Zn2+的存在使得BSA发射峰蓝移程度降低,猝灭常数、结合常数、结合位点数减小.在较大浓度Zn2+存在下,BR与BSA作用的相关系数增大,猝灭机制变为静态猝灭.从Zn2+与BR的竞争作用,热力学参数的变化、配位化合物的形成3个方面分析了影响BR与BSA作用的因素. 相似文献
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在模拟生理条件下,通过荧光光谱法研究染料木素(Ge)与牛血清蛋白(BSA)间相互作用,在水溶液中Ge能够有效地猝灭BSA的荧光发射。考查了溶液p H、水浴温度、水浴时间等参数对于Ge猝灭BSA的影响,并且通过StemVolmer方程计算了Ge与BSA之间的结合类型、结合位点数和结合常数等参数。结果表明,Ge猝灭BSA的类型为静态猝灭,Ge与BSA可形成1∶1型非共价复合物。通过同步荧光光谱法结合分子对接研究了Ge与BSA间的作用情况,结果表明,氢键作用与疏水作用是Ge与BSA形成复合物的主要驱动力,Ge通过改变BSA中Trp134残基微环境的疏水情况猝灭BSA的荧光发射。 相似文献
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M. Grehl R. Frhlich S. Thiele 《Acta Crystallographica. Section C, Structural Chemistry》1995,51(6):1038-1040
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Haloacetyl, peroxynitrates are intermediates in the atmospheric degradation of a number of haloethanes. In this work, thermal decomposition rate constants of CF3C(O)O2NO2, CClF2C(O)O2NO2, CCl2FC(O)O2NO2, and CCl3C(O)O2NO2 have been determined in a temperature controlled 420 l reaction chamber. Peroxynitrates (RO2NO2) were prepared in situ by photolysis of RH/Cl2/O2/NO2/N2 mixtures (R = CF3CO, CClF2CO, CCl2FCO, and CCl3CO). Thermal decomposition was initiated by addition of NO, and relative RO2NO2 concentrations were measured as a function of time by long-path IR absorption using an FTIR spectrometer. First-order decomposition rate constants were determined at atmospheric pressure (M = N2) as a function of temperature and, in the case of CF3C(O)O2NO2 and CCl3C(O)O2NO2, also as a function of total pressure. Extrapolation of the measured rate constants to the temperatures and pressures of the upper troposphere yields thermal lifetimes of several thousands of years for all of these peroxynitrates. Thus, the chloro(fluoro)acetyl peroxynitrates may play a role as temporary reservoirs of Cl, their lifetimes in the upper troposphere being limited by their (unknown) photolysis rates. Results on the thermal decomposition of CClF2CH2O2NO2 and CCl2FCH2O2NO2 are also reported, showing that the atmospheric lifetimes of these peroxynitrates are very short in the lower troposphere and increase to a maximum of several days close to the tropopause. The ratio of the rate constants for the reactions of CF3C(O)O2 radicals with NO2 and NO was determined to be 0.64 ± 0.13 (2σ) at 315 K and a total pressure of 1000 mbar (M = N2). © 1994 John Wiley & Sons, Inc. 相似文献
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Henry G. Giesber Michael B. Korzenski William T. Pennington Joseph W. Kolis 《Acta Crystallographica. Section C, Structural Chemistry》2000,56(4):399-400
The compound diiron diphosphate dihydrate, Fe2P2O7(H2O)2, was synthesized hydrothermally and crystallizes in the monoclinic space group P21/n. The compound has a somewhat open framework made up of edge‐sharing iron(II) octahedra that form chains connected by five bridging diphosphates. The remaining octahedral site of each iron is occupied by coordinated water. The H atoms of the water molecules all point into a common channel. 相似文献
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《The Journal of chemical thermodynamics》2007,39(11):1474-1492
High-temperature heat capacity measurements were obtained for Cr2O3, FeCr2O4, ZnCr2O4, and CoCr2O4 using a differential scanning calorimeter. These data were combined with previously available, overlapping heat capacity data at temperatures up to 400 K and fitted to 5-parameter Maier–Kelley Cp(T) equations. Expressions for molar entropy were then derived by suitable integration of the Maier–Kelley equations in combination with recent S∘(298) evaluations. Finally, a database of high-temperature equilibrium measurements on the formation of these oxides was constructed and critically evaluated. Gibbs free energies of Cr2O3, FeCr2O4, and CoCr2O4 were referenced by averaging the most reliable results at reference temperatures of (1100, 1400, and 1373) K, respectively, while Gibbs free energies for ZnCr2O4 were referenced to the results of Jacob [K.T. Jacob, Thermochim. Acta 15 (1976) 79–87] at T = 1100 K. Thermodynamic extrapolations from the high-temperature reference points to T = 298.15 K by application of the heat capacity correlations gave ΔfG∘(298) = (−1049.96, −1339.40, −1428.35, and −1326.75) kJ · mol−1 for Cr2O3, FeCr2O4, ZnCr2O4, and CoCr2O4, respectively. 相似文献
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Heinz Follner 《无机化学与普通化学杂志》1970,373(2):198-203
LiHC2O4 · H2O crystallizes in space group P 1 with a0 = 4.99, b0 = 6.16, c0 = 3.45 Å; α0 = 96.3°, β0 = 98.0°, γ0 = 80.4° and Z = 1. The crystal structure has been determined by direct methods. Refinement by least squares methods resulted to R1 = 8,3%. In the structure the oxalate group is not planar. The angle between the two O? C? O planes is 2.9°. 相似文献
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Neven Strukan Marina Cindri Boris Kamenar 《Acta Crystallographica. Section C, Structural Chemistry》2000,56(6):639-641
The crystal and molecular structure of dipotassium di‐μ‐oxo‐bis[aqua(oxalato‐O1,O2)oxomolybdenum(III)] trihydrate, K2[Mo2O4(C2O4)2(H2O)2]·3H2O, has been determined from X‐ray diffraction data. In the dimeric anion, which has approximate twofold symmetry, each Mo atom is in a distorted octahedral coordination, being bonded to one terminal oxo‐O atom, two bridging O atoms, two O atoms from the oxalato ligand and one from the water molecule. Bond lengths trans to the multiple‐bonded terminal oxo ligand are larger than those in the cis position, confirming the trans influence as a generally valid rule. 相似文献
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Rychlowska-Himmel I. Blonska-Tabero A. 《Journal of Thermal Analysis and Calorimetry》1999,56(1):205-210
Studies on the reactivity of ZnFe2O4 towards ZnV2O6 revealed that in the solid state the phases interact in a molar ratio of 1:3 to form a new compound, to which the molecular
formula Zn2FeV3O11 was assigned. The compound melts congruently at 825±5°C.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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U. Wannagat K. -P. Giesen H. -H. Falius 《Monatshefte für Chemie / Chemical Monthly》1973,104(5):1444-1452
Compounds I-X of the sixmembered ring system PSi2N2O with phosphorus in different oxidation and bond numbers, collected in Schema 1, have been prepared for the first time and confirmed in their structure by elemental analysis as well as by infrared and1H- and31P-spectroscopy.
Mit Auszügen aus der DissertationK. P. Giesen, Techn. Univ. Braunschweig 1972. 相似文献
Mit Auszügen aus der DissertationK. P. Giesen, Techn. Univ. Braunschweig 1972. 相似文献