共查询到20条相似文献,搜索用时 62 毫秒
1.
《高等学校化学学报》2015,(11)
表面增强拉曼散射(SERS)生物传感技术是以生物成分为敏感元件或探测对象,研究生物分子间相互作用的重要工具之一,被广泛应用于环境监测、食品安全、临床检验及疾病诊断等众多领域.本文总结了耦合增强SERS生物传感技术方面的进展及其在分析检测和癌症诊断方面的应用.主要包括基于耦合增强SERS生物传感技术方法、高灵敏度的SERS传感芯片的制备及其应用和新型SERS技术研究癌细胞及组织. 相似文献
2.
表面增强拉曼散射(surface-enhanced Raman scattering, SERS)技术以其独特的谱带窄、灵敏度高、抗光漂白、原位和无损等优势,在疾病诊断和生物分析领域得到了越来越广泛的应用。本文介绍了近几年来应用于生物大分子、病原微生物、细胞和活体检测分析中的最新SERS技术,并分别从标记与非标记的角度对其进行了阐述,总结了SERS标记检测生物大分子的基本识别模式,简述了检测低浓度病原微生物的SERS技术,着重评述了SERS检测技术在细胞和活体研究中的应用,并对基于SERS的疾病诊断和生物分析技术的发展趋势进行了初步展望。 相似文献
3.
4.
电化学生物传感技术以它独特的检测、分析方法以及在临床检测中潜在的应用,近年来受到研究者越来越多的关注。癌症生物标志物的早期检测能够使得患者在癌症发展至晚期前得到治疗,增加患者存活率。此外,生物标志物能够用于确定疾病的复发,以及患者在接受化疗、放疗和外科治疗之后的后续评估。本文主要论述了现有癌症生物标志物检测的设备和方法,并对这些方法的优点和不足作了简单的评述。另外,介绍了体外诊断设备的发展现状和电化学传感技术的特点,并对癌症早期的主要生物标志物进行了介绍,以及着重论述了电化学传感技术应用于临床靶向生物标志物检测的研究进展。此外,还展望了电化学生物传感技术未来的研究方向和发展趋势。从目前的的研究来看,电化学传感技术在体外诊断和临床检测癌症生物标志物等方面存在着较大的应用潜质,有望成为生物、医学、环境等领域重要的研究技术。 相似文献
5.
6.
microRNA是一段长约为18~24个核苷酸的内源性非编码单链RNA. 最新研究发现: 许多疾病和肿瘤的发生与microRNA的表达水平息息相关, 且microRNA有望成为新型肿瘤标志物及癌症治疗的新目标. 因此, 发展高灵敏度、高特异性及简单快速的microRNA分析检测方法对于生物医学研究和癌症的早期诊断具有重要的意义. 表面增强拉曼光谱(SERS)技术由于具有灵敏度高、检测速度快、指纹识别、水干扰小等独特优势, 在癌症的早期诊断领域具有很大的应用价值. 作者综述了SERS技术在microRNA检测方面的最新研究进展, 分析了该技术在生物检测中亟待解决的关键问题和挑战, 并对其未来的发展前景进行了展望. 相似文献
7.
8.
癌症是导致死亡的重要原因和提高预期寿命的严重阻碍。研究表明,早期的诊断和治疗是降低死亡率最有效的途径。肿瘤标志物的浓度变化通常与肿瘤发生、肿瘤细胞转移和临床治疗相关,肿瘤标志物检测对癌症的早期诊断,进展监测和预后具有重要的意义。近二十年来,基于电感耦合等离子体质谱(ICP-MS)检测肿瘤标志物的方法引起了研究者的广泛关注。ICP-MS具有分辨率高、灵敏度高、动态范围宽以及可多元素同时检测的优势。结合稳定同位素标记策略,已有许多基于ICP-MS的肿瘤标志物检测方法被报道。该文介绍了几种常见肿瘤标志物及其ICP-MS检测方法,并简要总结了ICP-MS在多组分生物标志物同时检测中的应用。 相似文献
9.
10.
11.
To explore the protein-level mechanism of action verapamil in acute myocardial infarcted rats, the myocardial proteome was
analyzed by two-dimensional electrophoresis (2-DE). Compared with the sham-operated group and the infarcted group, the result
shows that 8 protein expressions in the verapamil treated group were up-regulated, and 7 protein expressions in this group
were down-regulated significantly. Using MALDI-TOF-MS, 15 proteins with significant changes were identified through a database
search. These proteins can be divided into 4 groups by their biological function: (1) Energy metabolism and mitochondrial
function related proteins; (2) oxidative stress-induced proteins; (3) cytoskeletal Proteins; (4) other proteins. The findings
show that the myocardial protective effects of verapamil in the myocardial damage process are related to the recovery of energy
supply as well as anti-oxidative stress property.
__________
Translated from Chemical Journal of Chinese Universities, 2008, 29 (3) (in Chinese) 相似文献
12.
采用异丙肾上腺素诱导心肌缺血大鼠模型,使用液相色谱-质谱法检测血清和心肌中的内源性成分,应用软件对已鉴定的40余种目标成分进行靶向提取,用主成分分析(PCA)、有监督偏最小二乘法判别分析(PLS-DA)对代谢组学数据进行多维度统计分析,筛选潜在生物标志物。与对照组相比,在心肌缺血模型组大鼠血清、组织中检测出18个差异代谢物,涉及精氨酸和脯氨酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、谷氨酰胺和谷氨酸代谢、牛磺酸和亚牛磺酸代谢等多条代谢通路。代谢产物可作为心肌缺血研究中的重要标志物,该研究结果有助于揭示心肌缺血的发病机制,可为临床疾病诊断提供思路。 相似文献
13.
为探讨超声心动图联合动态心电图检查在急性心肌梗死(AMI)患者诊断和预后评估中的应用价值,本研究选取2016年6月~2018年6月我院收治并确诊的100例AMI患者作为观察组,另选取同期100例非冠心病患者作为对照组。以AMI患者心源性死亡为终点事件,将观察组分为死亡组(n=15)和存活组(n=85)。所有患者均采用飞利浦IU-Elite及EPIQ5彩色多普勒超声诊断仪进行超声心动图检查,采用DMS-3004A进行标准的12导联动态心电图检查。比较各组患者心率震荡指标[震荡起始(TO)、震荡斜率(TS)]、心率变异性指标[NN间期标准差(SDNN)、QT离散度(QTd)、经心率校正的QT离散度(QTcd)]及心功能指标[左心室舒张末期内径(LVEDD)、左心室射血分数(LVEF)]水平的变化。结果显示,观察组TO、QTd、QTcd、LVEDD均明显高于对照组,TS、SDNN、LVEF均明显低于对照组,差异有统计学意义(P<0.05)。死亡组TS、SDNN均明显低于存活组,差异有统计学意义(P<0.05);死亡组TO高于存活组,QTd、QTcd、LVEF、LVEDD均低于存活组,但差异无统计学意义(P>0.05)。本研究结果表明,AMI患者心率震荡(HRT)明显减弱甚至消失,HRV、LVEF明显降低,LVEDD、QTd明显增大,QT间期明显延长。超声心动图和动态心电图联合检查对AMI患者诊断及预后评估均有重要的临床应用价值。 相似文献
14.
由于PET在心肌灌注显像方面具有高分辨率、高灵敏度、低组织衰减和心肌血流定量等优势而使得PET心肌灌注显像剂的研究日益受到关注。本文介绍了目前已经应用于临床的PET心肌灌注显像剂的情况,分别综述了 18F 标记和其他正电子核素标记的PET心肌灌注显像剂的研究进展,重点讨论了几种亲脂性阳离子类和线粒体复合物Ⅰ(MC-Ⅰ)抑制剂类 18F 标记心肌灌注显像剂,特别是近年取得突破性进展的MC-Ⅰ抑制剂类心肌灌注显像剂(如BMS-747158-02和[18F]FP1OP),介绍了各类PET心肌灌注显像剂的生物性能、心肌摄取机制,对其优缺点进行比较,并对PET心肌灌注显像剂的应用前景进行了展望。 相似文献
15.
Lorenzo Tattini Pierandrea Lo Nostro Luca Scalise Barry W. Ninham Piero Baglioni 《Journal of colloid and interface science》2009,339(1):259-265
Diblock semifluorinated n-alkanes can form aggregates and gels in fluorinated solvents. We have investigated the thermal behavior of binary mixtures comprising F(CF2)8(CH2)16H and fluorinated solvents. The solvents were perfluorohexane, perfluoroheptane, perfluorooctane, perfluorooctyl bromide, perfluorodecalin, and perfluorotributylamine. The phase diagrams were used to calculate the activity coefficients of the two components and the main excess thermodynamic functions. The solubility and self-assembly behavior of F8H16 in the fluorinated solvents are related to the different solute–solvent dispersion interactions that depend on the polarizabilities and ionization potentials of the interacting species, and on the structural properties of the solvent. 相似文献
16.
17.
采用密度泛函理论(DFT)以及广义梯度近似方法(GGA)计算了甲酸根(HCOO)在Cu(110)、Ag(110)和Au(110)表面的吸附. 计算结果表明, 短桥位是最稳定的吸附位置, 计算的几何参数与以前的实验和计算结果吻合. 吸附热顺序为Cu(110)(-116 kJ·mol-1)>Ag(110)(-57 kJ·mol-1)>Au(110)(-27 kJ·mol-1), 与实验上甲酸根的分解温度相一致. 电子态密度分析表明, 吸附热顺序可以用吸附分子与金属d-带之间的Pauli 排斥来关联, 即排斥作用越大, 吸附越弱. 另外还从计算的吸附热数据以及实验上HCOO的分解温度估算了反应CO2+1/2H2→HCOO的活化能, 其大小顺序为Au(110)>Ag(110)>Cu(110). 相似文献
18.
The tripodal ligand 4-(2′-pyridylmthyl)-4-azaheptane-1,7-diamine has been prepared by reaction of 2-aminemethyl pyridine with acrylonitrile, followed by the reduction of the nitrile groups. Copper(II), nickel(II), zinc(II), cobalt(III) and chromium(III) complexes of the ligand have been prepared and characterized and the crystal structures of the complexes [CuLCl]ClO4 and [NiL(MeCN)2](ClO4)2 determined. The copper complex is five coordinate with approximate square pyramidal stereochemistry with the apical position occupied by a primary amine donor. The nickel complex is octahedral with the pyridine nitrogen donor lying trans to an acetonitrile ligand. 相似文献
19.
《Journal of Coordination Chemistry》2012,65(1-2):173-182
Abstract The reaction, in water, of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) ions with sodium ampicillinate at room temperature has allowed isolation of dimers with the following general formula [M(amp)Cl]2 × nH2O (n = 1.5?3.2). The complexes were characterized by elemental analyses, conductivity measurements, magnetic susceptibilities and spectroscopic methods (IR, Raman, EPR and UV-Visible). A dinuclear structure based on octahedrally coordinated metal ions is proposed. 相似文献
20.
Coordination compounds based on imidazole and benzimidazole substituted nitronyl nitroxide radicals with transition metal ions and trivalent lanthanide ions are described from the perspective of their magnetic properties.For the transition metal compounds the crystal structures show various metal-nitroxide dimensionalities including mononuclear (0D), one-dimensional (1D) and two-dimensional (2D) complexes. The mononuclear complexes were isolated with most metal ions of the first transition series. One copper(II) complex shows a copper(II)-radical ferromagnetic coupling (J = +75 cm−1) while for the other mononuclear compounds, mainly with manganese(II), the metal-radical interactions are antiferromagnetic. The one-dimensional and two-dimensional complexes are manganese(II) compounds which show canting effects leading to weak ferromagnetism.For the trivalent lanthanide ions [La(III), Gd(III) and Eu(III)], three series of mononuclear complexes were obtained in which the metal center is bound to four, two or one nitroxide radicals depending on the counter ions and ancillary ligands. Unexpectedly, in most gadolinium(III) complexes, the Gd(III)-radical interactions were found to be antiferromagnetic in contradiction with other foundings and previous theoretical models. In support to the magnetic studies, the optical properties of the lantanide complexes have also been investigated and are briefly described. 相似文献