氧化叔胺树脂的合成及其对苯酚的吸附性能研究

将D301树脂的叔胺基氧化，合成了大孔交联氧化叔胺树脂．比较D301树脂与氧化叔胺树脂对正己烷溶液中和水溶液苯酚的吸附性能，发现氧化叔胺树脂对苯酚的吸附量比D301树脂的有明显的增加．为弄清吸附量增加的原因，根据氧化叔胺树脂对正己烷溶液中苯酚的吸附等温线，利用热力学函数关系计算了等量吸附焓、吸附Gibbs自由能和吸附熵，发现叔胺树脂氧化后，与苯酚的相互作用和吸附的自发倾向增强，但吸附过程仍为氢键吸附．     

PYR树脂对甜菊糖的吸附与洗脱性能研究

系统研究了PYR型树脂对甜菊糖的吸附与洗脱性能，并与目前国内应用于甜菊糖提取分离的某些商品化吸附树脂进行了比较。结果表明，PYR树脂比其它树脂具有更优的性能，其静态吸附容量比AB—8树脂提高25％，且吸附速度快，易于洗脱再生，是一种具有工业化应用前景的新型吸附剂。     

AASI新型吸附树脂对芳香氨基酸的吸附、洗脱性能研究

用AASI新型吸附树脂来吸附苯丙氨酸、酪氨酸，研究了pH、温度，溶液中盐浓度对吸附性能的影响以及树脂结构与吸附性能间的关系，计算了树脂BET比表面的有效利用率。     

2-(2-噻唑偶氮)-5-[(N,N-二羧基甲基)氨基]苯甲酸浸渍树脂吸附金的特性及应用

用1300－Ⅱ型吸附树脂吸附噻唑偶氮类试剂制取浸渍树脂。研究了树脂对试剂和对金的吸附率及其与溶液pH值的关系，求得浸渍树脂吸附金的平衡速率和吸附容量，并在条件试验基础上拟订了柱操作分离富集微量金的方法，获得了满意的结果。     

几种树脂对芦荟苦素的吸附性能

研究硅胶H和三种不同极性的大孔吸附树脂对芦荟苦素的吸附与洗脱性能，用静态实验筛选树脂、动态实验考察芦荟苦素提取液浓度、上样流速和温度对GD-10大孔吸附树脂吸附能力影响。结果表明GD-10吸附树脂对芦荟苦素的吸附能力较强，洗脱能力最好，且易于再生。     

几种树脂对西红花糖苷的吸附性能的研究

本文系统研究了硅胶和几种国产吸附树脂对西红花提取物溶液的吸附与洗脱性能，包括树脂的筛选，洗脱剂的选择，并研究了pH，温度和西红花提取物的浓度对西红花提取在SZ－1吸附树脂上的吸附影响，结果表明，SZ－1吸附树脂对西红花提取物的吸附能力较强，洗脱能力为最好，其吸附性能和洗脱性能均优于常用色谱固定相硅胶，SZ－1吸附树脂的静态吸附容量为1．04g/g，动态吸附容量为0．5－0．6g/g，洗脱率可达98％以上，且吸附速度快，易于洗脱和再生。     

AAS新型吸附树脂对苯丙氨酸、酪氨权的吸附分离性能的研究

研究了AASⅠ、AASⅡ两系列新型吸附树脂对苯丙氨酸、酪氨酸的附分离性能，分析了树脂结构与吸附分离性能间的关系，探讨了聚苯乙烯系吸附树脂与芳香氨基酸间的作用机理。     

吸附树脂对模拟氰化液中Au（Ⅰ）的吸附性能研究

测定了HW型吸附树脂对模拟氰化液中Au(Ⅰ)的静态吸附能力，研究影响吸附的一些因素。结果表明，HW树脂在中性条件下对Au(Ⅰ)的吸附量与活性炭接近，碱性条件下吸附量降低较多。某些胺的存在可使树脂吸金量提高十倍。树脂的解吸性能良好，解吸效率接近100％。     

酚醛型吸附树脂对茶碱的吸附性能研究

研究了酚醛型吸附树脂对茶碱的静态和动态吸附。结果表明3种树脂对茶碱的吸附量均达117－204mg/g，明显优于DuoliteS-761;酚醛型吸附树脂等温吸附茶碱的平衡吸附数据符合Langmuir方程，相关系数在0．99以上，因此，酚醛型吸附树脂吸附茶碱属单分子层吸附；用1mol/LHCl和W甲醇为80％复合溶液作为吸附树脂的洗脱剂，效果很好。     

笼形聚偕胺肟树脂的研究─—H_2PtCl_6在酸处理树脂上的吸附过程

H2PtCl6可被一系列的酸处理定形聚偕胺肟树脂所吸附，吸附容量的大小与处理树脂的质子酸的Pka值有关．认为是解高度低的酸所处理的树脂上的伯胺基成盐的机会较少，即树脂的吸附中心保留着较多的空穴，有利于H2PtCl6的接近而被吸附下来．吸附动力学的研究表明化学反应是吸附速度的控制因素。     

改性木粉填充柱对茶多酚与咖啡因分离应用的研究

用乙基纤维素、己二酸和丙烯酰胺制成浆液改性剂,对木粉进行改性.研究对比了木粉在改性前后对茶多酚(TP)与咖啡因(Caf)的分离效果.研究结果表明,已改性的木粉对TP中3种主要成分(EGC,EGCG和ECG)与Caf的分离效果优于未改性木粉.改性后的木粉可用于TP各组分与Caf分离时的前处理.通过该前处理可将绿茶提取液分离为两部分:一部分主要由咖啡因和表没食子儿茶素(EGC)组成,另一部分主要由表没食子儿茶素没食子酸酯(EGC)和表儿茶素没食子酸酯(ECG)组成.     

胶原纤维吸附材料除去茶多酚中的咖啡因

以皮胶原纤维制备吸附材料，研究了这种吸附材料对茶多酚中各组分的吸附特性．结果表明，所制备的吸附材料能选择性地吸附EGCG、GCG和ECG，吸附率在90％以上；对C、EC和EGC的吸附率相对较低；对咖啡因的吸附率最小，只有11．24％．吸附后的产物经纯水淋洗和丙酮水溶液洗脱后，EGCG、GCG和ECG的总含量达到93.81％，回收率均在80%以上；而咖啡因的含量只有0．038％．进一步研究表明，皮胶原纤维吸附材料对茶多酚中不同组分的选择吸附能力与各组分的分子结构有关．     

Improved sample pre-treatment for determination of caffeine in tea using a cartridge filled with polyvinylpolypyrroridone (PVPP)

We have improved sample pre-treatment for the effective removal of polyphenols and simple analysis of caffeine in tea using a cartridge filled with polyvinylpolypyrroridone (PVPP). Nearly 100% of catechins were removed from the green tea sample and caffeine was completely recovered in the range of 98.2-101.3% by sample pre-treatment with a PVPP cartridge. Reproducibility of preparing PVPP pre-treatment cartridges was sufficient for quantitative analysis, because RSDs of analytical values for caffeine obtained by using three individual pre-treatment cartridges filled with 10-200 mg PVPP were 0.60-2.8%. The PVPP pre-treatment cartridge also removed polyphenols perfectly and recovered caffeine faultlessly from oolong and black tea samples. Comparison with the conventional method without sample pre-treatment indicated that the present pre-treatment method with a PVPP cartridge was useful for the simple and precise analysis of caffeine in green, oolong and black tea samples.     

Simple and rapid UV spectrophotometry of caffeine in tea coupled with sample pre-treatment using a cartridge column filled with polyvinylpolypyrrolidone (PVPP)

We have applied a sample pre-treatment method with a cartridge column filled with polyvinylpolypyrrolidone (PVPP) to the effective removal of polyphenols and simple UV spectrophotometry of caffeine in tea. The absorption maximum length (lambda(max)) for caffeine was close to those for tea catechins in aqueous 1% acetic acid; therefore, the UV spectrum of a non-treated green tea sample had a large absorption wave. In contrast, the absorbance of the green tea sample was gradually reduced by PVPP cartridge treatment using PVPP from 0 to 50 mg, and was nearly constant using a pre-treatment cartridge with more than 100 mg PVPP, because tea catechins were effectively removed and caffeine was mostly recovered from a green tea sample by means of PVPP cartridge treatment. The PVPP pre-treatment cartridge also removed polyphenols successfully from oolong and black tea samples. Comparison with conventional HPLC analysis indicated that the present pre-treatment method with a PVPP cartridge was useful for the simple and selective UV spectrophotometric determination of caffeine in green, oolong and black tea samples.     

反相高效液相色谱法直接测定茶叶水提取物中的嘌呤碱

 用反相高效液相色谱法直接测定茶叶水提取物中的咖啡因、可可碱和茶碱,方法简便快速。在２７０ｎｍ检测波长下,可可碱、茶碱和咖啡因的检测下限分别为０７,０９和１８ｍｇ／Ｌ,峰面积标准曲线在６～１０００ｍｇ／Ｌ范围内具有良好的线性关系,线性相关系数为０９９８以上。     

Rapid determination of caffeine in tea leaves

For the purpose of efficient screening of low-caffeine tea shoots, a method for the rapid determination of caffeine was developed using high-performance liquid chromatography. Polyvinylpolypyrrolidone was packed in a pre-column and used to remove polyphenols from tea extracts on-line. The concentrations of caffeine extracted from powdered tea leaves at 50 degrees C during 1 day could be analyzed in 2-5 min intervals. The pre-column and the analytical column could be used for the analysis of more than 2000 samples.     

Rapid, direct determination of polyphenols in tea by reversed-phase column liquid chromatography.

Column liquid chromatography on a C18-bonded silica column with water-methanol-acetic acid as eluent was used to determine polyphenols and caffeine in tea. Without any pretreatment, catechin, epicatechin gallate, epigallocatechin gallate, epigallocatechin, epicatechin and caffeine were separated successfully within 15 min. The detection limits (S/N = 3) of polyphenols studied were 1.8-24 mg/l at a detection wavelength 270 nm. The linear range of the peak area calibration curves for the analytes were over two orders of magnitude with a correlation coefficient of 0.996-0.999. Using this method, some Chinese tea samples were analyzed with a good reproducibility (RSD are below 5%).     

Rapid analysis of methylated xanthines in teas by an improved high-performance liquid chromatographic method using a polyvinylpolypyrroridone pre-column.

We have developed a new high-performance liquid chromatography (HPLC) method for the analysis of methylated xanthines in tea by removing polyphenols using a polyvinylpolypyrroridone (PVPP) pre-column. The PVPP pre-column was connected with the upstream of analytical column to remove catechins in tea extract. Using this pre-column, caffeine and theobromine in tea, which belong to methylated xanthines, could be rapidly determined in less than 10 min with an isocratic solvent system. RSDs of standard solutions of caffeine and theobromine were about 0.3 and 0.3% for the retention time, and were about 1.6 and 2.5% for the peak area. The quantitation curves of caffeine and theobromine were linear from 5 to over 1000 ng.     

蔗汁酒精废液色素的提取及其抗氧化活性研究

针对蔗汁酒精废液中含有的大量色素物,采用大孔树脂吸附提取色素,对蔗汁酒精废液中色素的提取工艺及其抗氧化活性进行了研究。通过静态吸附实验筛选出吸附树脂D101及对应的洗脱溶剂乙醇。最佳流速为3BV/h,最佳洗脱条件为40%的乙醇在pH6～7范围内进行洗脱。经测定,色素的黄酮含量为茶多酚的27.2%,但对自由基的抑制率却为茶多酚的88.06%,表明蔗汁酒精废液中提取的色素具有较高的抗氧化活性。     

Classificatin of chinese tea samples according to origin and quality by principal component techniques

Three categories of tea, black, green and oolong tea, with those varieties in each category were analyzed for their contents of cellulose, hemicellulose, lignin, polyphenols, caffeine and amino acids. The data were subjected to multivarige analysis. Principal component analysis and principal component classification provide discrimination between the different categories and varieties. The quality index, assigned to these tea standards by experts, could be predicted form the principal component scores.