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1.
荧光光谱法研究β—环糊精与L—酪氨酸的主客体包络反应   总被引:3,自引:0,他引:3  
以荧光光谱法系统地研究了水溶液中β-环糊精与L-酪氨酸的主客体包络反应条件,包络物的荧光光谱性质,利用改进的Benesi-Hildebrand法测定了包络物的形成常数,初步了探讨了某些水溶性一元醇的存在对该包络物形成常数及荧光性质的影响。检出限为2×10^-7mol/L,平均回收率为99.1%。  相似文献   

2.
β环糊精的双核铜络合物对芳香基氨基酸分子识别的研究   总被引:3,自引:0,他引:3  
用荧光分光光度法和紫外分光光度法测定了β-环糊精双核铜络合物(Cu2-β-CD)在强碱性溶液中与氨基酸的超分子作用,发现Cu2-β-CD与芳香基氨基酸的荧光光谱和紫外光谱发生明显变化,Cu2-β-CD对于芳香基氨基酸具有分子识别作用,对于酪氨酸和苯丙氨酸,主客体间形成超分子络合物的化学计量比为1:4.有Scatchard方程或Lineweaver-Burk曲线分别了它们的物理常数,推测Cu2-β2  相似文献   

3.
本文报道一种新型的重原子微扰剂-环氧氯丙烷(ECH),在此重原子微扰下,建立了β-环糊精诱导室温磷光法(β-CD-RTP)测定痕量溴代萘(BrN)的方法,结果令人满意。同时还在该体系中测定了溴代萘的DTP寿命、猝灭常数、三元包合物的包接常数。实验结果证明,α-BrN/ECH/β-CD三分子包接配合物比β-BrH/ECH/β-CD三分子包接络合物包接更加有效,而且稳定性更强。  相似文献   

4.
李益民  戚文彬 《分析化学》1994,22(6):548-551
本文从β-环糊精(β-CD)的包合作用及其对表面活性剂(SF)包合后引起的包合常数增大等方面,探讨了β-CD-与离子表面活性剂对显色反应的协同增敏作用机理,研究表明:β-CD与SF形成包合物SF-β-CD及SF-β-CD对显色剂及其显色络合物包合作用的增强,是产生协同增敏作用的主要因素,但还须考虑对空白值的影响以及配合物的稳定性,还提出了对一同显色体系应用不同SF-β-CD估计协同增敏趋势的方法。  相似文献   

5.
对β-环糊精(β-CD)溶液中4种多环芳烃α-溴代萘(α-BrN)、β-溴代萘(β-BrN)、菲、敏化丁二酮(BIAC)的室温光(RTP)进行了研究,β-CD能有效地增强敏化光强度,并从三重态能量、主客体分子几何尺寸大小等方面讨论了各种因素的影响。方法的线性动态范围上限受能量受体浓度的限制,其检出限达 10-8mol/L。  相似文献   

6.
苏小笛  朱敏 《应用化学》1997,14(3):33-36
本以β-环糊精(β-CD)和表面活性剂为增效剂,分别研究了它们对以Fe-meso-(四(4-磺基苯)卟啉)(Fe-TPPS4)为催化剂,催化过氧化氢氧化4-氨基安替比林(4-AAP)与苯酚衍生物显色反应的速度和灵敏度的影响,发现β-CD和十二烷基苯磺酸钠(SDBS)对该体系具有明显的增效作用,在3×10^-3mol/Lβ-CD存在下,测定H2O的灵敏度比献报道的提高了1.56倍,利用SDBS深  相似文献   

7.
高春光  谢剑炜 《分析化学》1998,26(12):1424-1227
对β-环糊精(β-CD)溶液中4种多环芳烃α-溴代萘(α-BrN)、β-溴代萘(β-BrN)、菲、Qu敏化丁二酮(BIAC)的室温磷光(RTP)进行了研究,β-CD能有效地增强敏化磷光强度,并从三重态能量、主客体分子几何尺寸大小等方面讨论了各种因素的影响。方法的线性动态范围上限受能量受体浓度的限制,其检出限达10^-8mol/L。  相似文献   

8.
疏水条件下甾体化合物的分离及环糊精的作用   总被引:2,自引:0,他引:2  
分别应用胶束电动毛细管色谱法(MECC)、反相色谱法和串联柱HPLC法分离 左旋十八甲基炔诺酮(LNG)、睾酮(T)、孕酮(P-雌二醇(E2)等甾体化合物MECC分离在未涂层毛细管柱上进行。当电泳缓冲溶液为含20mmol/L的2,6-二甲基-β环糊精((DM-β-CD)添加剂和50mmol/L的十二烷基硫酸钠(SDS)的10mmo/L四硼酸钠(PH9.2)时,可实现快速分离。通过与反相色谱法和串联  相似文献   

9.
毛细管电泳测定主客分子相互作用的结合常数   总被引:5,自引:0,他引:5  
朱晓峰  林炳承 《分析化学》1999,27(12):1408-1411
在25℃下,用毛细管区带电泳测定了主管体分子(配体和溶质分子)β-环糊精(β-CD)和心得舒(alprenolo)在pH值为2.5,浓度为100mmol/L的磷酸盐缓冲溶液下的结合常数,并对4种求解方法既非线性拟合法,双倒数法,y-倒数法和x-倒数法的结果进行了比较,所得值分别是307.2、408.4、331.4和343.1L/mol,同时获得了结合物的迁移率。该方法可用于测定结合比为1:1的各种  相似文献   

10.
应激光闪光光解瞬态吸收光谱研究了水溶液中含芳香氨基酸残肽的光敏化反应过程,结果表明,在丙酮存在的含色氨酸残基肽(Trp-Gly,n-f-Met-Trp,Trp-Phe)体系的光解,丙酮三重态与Trp分别通过三记-三重态(T-T)激发能转移和电子转移生成Trp激发三重态和N中心自由基(Trp/N^*);丙酮三重态仅与含酷氨酸残基肽(Phe-Tyr)通过电子转移生成Tyr酚氧自由基(Tyr/O^*)。在色氨酰酪氨酸(Trp-Tyr)与丙酮的光解体系中,观察分子内的电子转移,即由Trp/N^.-Tyr→Trp-Tyr/O自由基的生成过程。  相似文献   

11.
The experimental procedures to place poly(ethylene 2,6‐naphthalate) (PEN) guest molecules within γ‐cyclodextrin (γ‐CD) host molecules are described along with the subsequent verification of inclusion‐compound (IC) formation. In addition, the simultaneous complexing of PEN and poly(ethylene terephthalate) (PET) with γ‐CD to form their common IC is documented. Coalescence from their common γ‐CD IC generates an intimate blend of the PET and PEN polymers contained therein. Thermal analysis via differential scanning calorimetry reveals thermal behavior indicative of an intimate blend of PET and PEN. 1H NMR analysis confirms that the intimate blending of PET and PEN achieved by coalescence from their common γ‐CD IC is not due to transesterification into a PET/PEN copolymer during thermal analysis. © 2002 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 41: 139–148, 2003  相似文献   

12.
虫草素与羟丙基-β-环糊精的包合行为及性能研究   总被引:1,自引:0,他引:1  
通过饱和溶液方法制备了虫草素(COR)与羟丙基-β-环糊精(HPβCD)形成的包合物.采用紫外-可见光谱法对水溶液中HPβCD与虫草素(COR)的包合行为进行研究,利用Job曲线法确定COR/HPβCD包合物的包合比,通过1 H NMR和2D NMR、DSC、TG、XRD、FTIR和SEM对COR/HPβCD包合物进行表征和性能测定.结果表明,COR/HPβCD包合物的包合比为1:1,虫草素与HPβCD形成包合物后,其水溶性、热稳定性及生物环境稳定性都得到明显提高.COR/HPβCD包合物在医药领域具有潜在的应用前景.  相似文献   

13.
A new cyclodextrin derivative with the anion side-arm, mono[6-deoxy-6-(2-sodium thio-1,2-dicyane ethylenylthio)]-cyclodextrin (6-mnt-CD), and its inclusion complex with ferrocene (6-mnt-CD/Fc), have been prepared and characterized by elemental analysis, IR spectroscopy, UV spectroscopy, mass spectrometry, 13C-NMR spectroscopy, thermogravimetry, and cyclic voltammetry (CV). Thermogravimetry analysis of the compound shows that the thermal stabilities of both the host and the guest in the inclusion complex have been greatly improved due to an additional interaction between the side-arm of the host and the guest. The interplay between the guest and the host with side-arm in the complex resulted in smaller positive potential shifts in CV compared to that in the inclusion complex CD/Fc.  相似文献   

14.
Abstract

The chemical modified β-cyclodextrin(CD), which bears a fluorophore group — N,N-dimethylamino-chalcone (DMAC), was synthesized. The self-inclusion behavior of this compound in aqueous solution and in alcohol/water mixed solvents was investigated in detail, and compared with the inclusion system of non-modified CD with free DMAC molecule. The results showed that a deep self-inclusion complex was formed for this compound in aqueous solution and had the ability to recognize different organic molecules by two fully different modes: “in-out” and “co-inclusion” mechanisms. The inclusion behavior of these systems in various ratio of alcohol/water mixture as solvents was investigated. The results indicated that the self-inclusion complex has a higher stability in alcohol/water mixed solvents than that in the case of non-modified CD. The chalcone group appended at β-CD enabled the host as a sensitive probe to study the inclusion behavior of CD.  相似文献   

15.
The crystal structures of the -phase of hexakis(3-hydroxy-3,3-diphenylprop-2-ynyl)benzene (1), and its inclusion compound with acetonitrile and benzene (2) have been determined by single crystal diffraction. 2 was further characterised by nuclear magnetic resonance spectroscopy, and thermal analysis. The inclusion of benzene by this host appears to depend on the presence of acetonitrile.  相似文献   

16.
以水溶性磷酸盐柱[6]芳烃(WP6P)为主体, 天然药物甘松新酮(ND)为客体, 采用饱和溶液法构筑了一种新的ND/WP6P主客体包合物. 通过紫外光谱滴定法研究了主客体之间的络合行为, 结果表明, ND和WP6P以摩尔比1∶1形成主客体包合物, 其络合常数为5.160×104 L/mol. 利用红外吸收光谱、 X射线粉末衍射、 热重分析(TG)、 示差扫描量热分析(DSC)和扫描电子显微镜对形成的包合物进行了表征. 通过一维和二维核磁共振氢谱及分子对接模拟等方法进一步验证了ND与WP6P之间形成主客体包合物的包合模式. 本文为水溶性柱芳烃在天然药物方面的应用提供了理论参考.  相似文献   

17.
Resveratrol (Res) is a plant-based polyphenol compound and is known to inhibit the growth of a variety of cancer cells and protect lipoproteins against oxidative damage. However the poor solubility and labile property may constitute a serious problem for its bioavailability. The problem could be overcome by the formation of inclusion complexes with cyclodextrins (CDs). The aim of this work is to include Res by β-cyclodextrin (β-CD) and 2-hydroxypropyl-β-cyclodextrin (HP-CD) to form the Res/β-CD and Res/HP-CD inclusion complexes and evaluate their cytotoxicity on cancer cells and inhibition of lipid peroxidation activity. The complexes are characterized by powder X-ray diffraction, fourier transform infrared spectroscopy and scanning electron microscopy. The cytotoxicity of the two complexes has been evaluated by methylthiazoletetrazolium reduction assay on two cancer cell lines (cervical carcinoma cells HeLa and hepatocellular liver cancer cells Hep3B) and one normal cell line (umbililical vein endothelial cell HUVEC). The results showed that the two complexes exhibit high cytotoxicity on two cancer cells, especially for Hep3B, and show no significant effect on normal cells. The Res/HP-CD complex shows higher cytotoxicity on the two cancer cells than that of the Res/β-CD complex. The inhibition of lipid peroxidation induced by Fe2+/ascorbate of the two inclusion complexes has been determined by thiobarbituric acid assay. The inhibition rate shows a linear increase with the increase of CDs concentration, and the Res/HP-CD complex shows stronger inhibition activity than that of the Res/β-CD complex. The results of this work indicate a potential for using the Res/CD complexes to inhibit human cancer growth and lipoproteins peroxidation.  相似文献   

18.
A new host design for an inclusion compound with a preference for large planar aromatic guest molecules has been proposed. Our host design includes a rectangular cavity made using a long and a short building block based on the concept of supramolecular chemistry. The long building block facilitates the inclusion of large guests, and the short building block prevents the formation of an interpenetrated structure, which is often observed in frameworks with large void spaces. The long building block is made when dimers of 4-pyridinecarboxylic acid (isoH) form through hydrogen bonding between the two carboxylic acid moieties. This isoH dimer can link two transition metal centers using the N atoms at both ends to act as a long building block. For the short building block, the thiocyanato ion was used. This makes a bent bridge between two metal centers to form a 1D double-chain [M(SCN)2]infinity complex. From the self-assembly of isoH, SCN- and Ni2+, a 2D network of [Ni(SCN)2(isoH)2]infinity, in which the 1D [Ni(SCN)2]infinity complexes are linked by the isoH dimers, is built up. The rectangular cavity is formed as a mesh within the 2D network. The crystal of our inclusion compound has a layered structure of 2D networks, and a 1D channel-like cavity penetrating the layered 2D networks is formed where guests may be included. Moreover, our host design has the advantage of easy extension of the host structure. Replacement of isoH with another component and use of three components is possible for making the long building block. In the latter case, a linear spacer having two carboxy groups is inserted into the isoH dimer to form a long building block with a trimer structure. Based on our host design, a series of new inclusion compounds were synthesized. The crystal structures of three compounds were determined by single crystal X-ray diffraction. These were a biphenyl inclusion compound [Ni(SCN)2(isoH)2].1/2C12H10 (the basic case), a 9,10-dichloroanthracene inclusion compound [Ni(SCN)2(acrylH)2].1/2C14H8Cl2, where isoH is replaced with 3-(4-pyridinyl)-2-propenoic acid (acrylH), and a perylene inclusion compound [Ni(SCN)2(isoH)2(fumaricH2)].1/2C20H12, whose long building block is a trimer inserted with fumaric acid (fumaricH2) as a linear spacer.  相似文献   

19.
Poly(dimethylbutadiene) (PDMB) was synthesized through the inclusion polymerization technique, by γ-irradiation of a clathrate of 2,3-dimethyl-1,3-butadiene in deoxycholic acid (DOCA) at 75, 150, 320 and 430 kGy. The resulting inclusion complexes PDMB@DOCA were studied by FTIR spectroscopy and by thermal analysis (DTA, TGA and DTG). Pure PDMB was isolated by extracting the complex PDMB@DOCA with ethanol. The best sample in terms of purity was that prepared at 75 kGy, while the other samples prepared at higher doses suffered of DOCA grafting on PDMB chains. Pure PDMB isolated from PDMB@DOCA complex was studied by FT-IR spectroscopy and by thermal analysis in comparison to a reference highly crystalline and ≈=100% trans-1,4-PDMB prepared by inclusion polymerization in thiourea and in comparison to PDMB prepared by emulsion polymerization. A lower degree of regularity and crystallinity has been found on the PDMB sample prepared as inclusion compound in DOCA in comparison to the reference PDMB obtained by inclusion polymerization in thiourea.  相似文献   

20.
The purpose of the study was to evaluate an octanol-water phase distribution method for investigation of drug/cyclodextrin (D/CD) complexes and to compare stability constant values obtained by this method to values obtained by the phase solubility method. A general equation for determination of 1 : 1 D/CD complex stability constant (K1 : 1) from the slope of a phase-distribution diagram (a diagram of the reciprocal of the apparent partition coefficient vs. the total CD concentration) was derived. The equation accounted for the possible inclusion of the organic solvent in the CD cavity and the gradual saturation of the CD binding with increasing concentration of the guest compound. This method was used to determine K1 : 1 for 2-hydroxypropyl-beta-cyclodextrin (HPbetaCD) complexes of hydrocortisone, prednisolone, diazepam, beta-estradiol and diethylstilbestrol. These values were comparable to K1 : 1 values determined by the phase-solubility method. The phase-distribution method could also be applied to determine stability constants for the neutral and ionic forms of the weakly acidic drugs, naproxen and triclosan and the weakly basic drug lidocaine. The phase-distribution method is a very versatile and fast method and has the advantage, compared to the phase-solubility method, that it only requires very small drug samples. Thus, this method would be suitable for screening of new drug candidates.  相似文献   

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