Lipids of microalgae V. Composition of the lipids of the biomass ofMonochrysis lutheri grown with periodic illumination

It has been shown that in the biomass ofMonochrysis lutheri grown under the conditions of an accumulation culture with periodic illumination the level of total lipids, including the majority of classes of glycolipids and some phospholipids, is lower than on continuous illumination. In the fatty acids of the triacylglycerols the amounts of the 20:4 and 20:5 species were somewhat lower while the amounts of polar lipids had increased.     

Lipids of Some Lycopodiophyta Species

The fractional composition of neutral and polar lipids from four Lycopodiophyta species was studied. It has been established that the neutral lipids consist of esters of glycerin, alcohols, fatty acids, sterols, waxes, and hydrocarbons. The polar lipids contain glycolipids, phospholipids, and the betaine lipid of DGTS.     

Chromatographic Analysis for Fatty Acids and Lipid-Soluble Bioactives of Derris indica Crude Seed Oil

A combination of column chromatography (CC), gas chromatography (GC), thin layer chromatography (TLC) and liquid chromatography (LC) techniques were performed to analyze lipid classes, fatty acids and fat-soluble bioactives of Derris indica crude seed oil. Hexane extract of Derris indica oilseeds was found to be 56%. Level of neutral lipids in the crude seed oil was the highest, followed by glycolipids and phospholipids, respectively. Linoleic followed by α-linolenic, palmitic and oleic were the major fatty acids in the crude seed oil. The ratio of unsaturated fatty acids to saturated fatty acids was higher in neutral lipid classes than in the polar lipid fractions. The oil was characterized by a relatively high amount of phytosterols, wherein the sterol markers were β-sitosterol, campesterol and stigmasterol. γ-Tocopherol was the major tocopherol while the rest being α-tocopherol. In consideration of potential utilization, detailed knowledge on the composition of Derris indica oil is of major importance.     

Lipids of the tuberous roots ofMandragora turcomanica

The lipids of the tuberous roots ofMandragora turcomanica Mizger (fam. Solanaceae) have been characterized for the first time. By using CC, TLC, Ag⁺-TLC, UV and IR spectroscopies and mass spectrometry, 20 classes of lipids and lipophilic substances have been characterized. The main components of the acyl-containing lipids that were isolated were found to be triacylglycerols and glycolipids, and among the lipophilic compounds they were alkanols and sterols; in the mixture of fatty acids of the acyl-containing lipids the 18:2, 18:1, and 16:0 acids predominated.     

Lipids ofHeracleum lehmannianum seeds

The lipid complex of the seeds ofHeracleum lehmannianum B. has been studied. The qualitative and quantitative compositions of the neutral lipids and the phospho- and glycolipids, and the fatty acid compositions of the acyl-containing classes of lipids have been determined. It has been shown that the neutral and the polar lipids differ considerably from one another with respect to the level of certain fatty acids. The position-species compositions of the TAGS and of the main components of the phospholipids have been calculated from the results of lipolytic hydrolysis.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 10–14, January–February, 1996. Original article submitted June 12, 1995.     

Lipids and flavolignans from <Emphasis Type="Italic">Silybum marianum</Emphasis>

Lipids from the air-dried aerial part (AP) and seeds of Silybum marianum (L.) Gaerth. (Asteraceae) were studied. The class and fatty acid compositions of neutral lipids (AP, seeds) and glyco- and phospholipids (AP) were determined. Neutral lipids (NL) with a complicated set of lipophilic components, mainly triterpenols, sterols, and their esters predominated in the AP. The fatty acids of the AP were dominated by 16:0, 18:2 (glycolipids), and 18:3 (neutral lipids, phospholipids); of seed NL, by 18:2 and 18:1. The content and composition of flavolignans isolated from defatted seeds and the content of total protein in the meal were found.     

Lipids of two species of brown algae of the genusLaminaria

The compositions of the neutral lipids, glycolipids, phospholipids and fatty acids of the brown algaeLaminaria japonica andLaminaria cichoriodes have been studied. It has been found that the glycolipids are the main lipid classes in these algae. Palmitic, oleic, and polyunsaturated acids with 18 and 20 carbon atoms form the group of main acids. The lipids of both species of theLaminaria genus have a complex composition but they differ by the ratios of their fatty acids.Institute of Marine Biology, Far-eastern Scientific Center, Russian Academy of Sciences, Vladivostok, fax (4232) 310900, e-mail: lipid@biom.marine.su. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 22–26, January–February, 1999.     

Lipids of the fruit of Ficus carica

By chromatographic methods, about 30 groups of various lipid compounds belonging to the classes of neutral lipids, glycolipids, and phospholipids have been identified from the fruit of the fig tree. The main groups are triacylglycerols, free and esterified sterols, mono- and digalactosyldiglycerides, ceramide oligosides, cerebrosides, esterified sterol glycosides, and phosphatidylglycerols. In the fatty acid composition, linoleic, linolenic, oleic, and palmitic acids predominated (>90%).     

Post-infection changes in lipids of the roots of a cotton plant variety moderately resistant to Verticillium dahliae

A comparative study has been made of the lipid composition of healthy roots of the cotton plant of the Tashkent-1 variety (I) and of the corresponding plants infected withVerticillium dahliae Kleb. (II) grown under field conditions. The action of the factors of infection stress and seasonal and climatic conditions change the lipid metabolism. In lipids (I) and (II) seven classes of neutral lipids (NLs), three classes of glycolipids, and six classes of phospholipids (PLs) were identified and their fatty acid compositions were determined. It was established that in (II) there was a smaller amount of total lipids, NLs, and PLs, and an increased level of polyphenolic pigments, but the level of gossypol pigments with free aldehyde groups was unchanged. In the NLs of (II), as compared with (I), there were more triacylglycerols, sterols, triterpenols, and squalene. Complex changes were observed in the fatty acid composition of the acyl-containing lipids.Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 59–63, January–February, 1989.     

Composition of the phospholipids of the seeds of Spaium sebiferum

The fractional composition of the polar lipids and also the fatty-acid composition of the main components of the phospholipids of the Chinese tallow tree have been studied. It has been established that in the seeds of the Chinese tallow tree there are 11 phospholipids, two glycolipids, and two phosphonolipids. The main fatty acids of the phospholipids are palmitic and linoleic.I. G. Kutateladze Institute of Pharmatochemistry, Georgian Academy of Sciences, Tbilisi. Institute of Chemistry of Plant Substances, Uzbek Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 619–621, September–October, 1991.     

Lipid content and fatty acid composition in lemon wax

Lipids were extracted from lemon wax and fractionated into four classes on a silicic acid glass packed column by thin-layer chromatography (TLC). The free fatty acids, the fatty acid composition and the amount of each separated lipids were determined by capillary column gas chromatography (GC). Total lipids (TL) were 60 mg per 100 g raw weight and the ratio of nonpolar lipids (NPLs): glycolipids (GLs): phospholipids (PLs) was about 47:2:2. The main free fatty acids in lemon wax were hexadecanoic acid, cis-9-octadecenoic acid and cis,cis-9,12-octadecadienoic acid, while in the lipid fractions the main fatty acids were hexadecanoic acid in all the fractions, cis-cis-9,12-octadecadienoic and decanoic acids in triglyceride (TG) fraction, dodecanoic and cis-9-octadecenoic acids in diglyceride (DG) fraction and tetradecanoic, octadecanoic and cis-9-octadecenoic acids in GL and PL fractions. The ratio of unsaturated to saturated fatty acids showed a remarkable difference among these four lipid fractions. In PL and GL fractions this ratio was similar, 47.7% and 47.1% respectively, and in TG fraction it was 42.4% while in DG fraction this value was 23.5%.     

Composition of the phospholipids of the seeds of Spaium sebiferum

The fractional composition of the polar lipids and also the fatty-acid composition of the main components of the phospholipids of the Chinese tallow tree have been studied. It has been established that in the seeds of the Chinese tallow tree there are 11 phospholipids, two glycolipids, and two phosphonolipids. The main fatty acids of the phospholipids are palmitic and linoleic. I. G. Kutateladze Institute of Pharmatochemistry, Georgian Academy of Sciences, Tbilisi. Institute of Chemistry of Plant Substances, Uzbek Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 619–621, September–October, 1991.     

Lipids of microalgae V. Composition of the lipids of the biomass ofMonochrysis lutheri grown with periodic illumination

It has been shown that in the biomass ofMonochrysis lutheri grown under the conditions of an accumulation culture with periodic illumination the level of total lipids, including the majority of classes of glycolipids and some phospholipids, is lower than on continuous illumination. In the fatty acids of the triacylglycerols the amounts of the 20:4 and 20:5 species were somewhat lower while the amounts of polar lipids had increased.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 601–604, September–October, 1994.     

Separation of lipid classes from plant tissues by high performance liquid chromatography on chemically bonded stationary phases

The wide range of lipid classes found in plant tissues, including simple lipids, glycolipids, and phospholipids, have been resolved by gradient high performance liquid chromatography with evaporative light-scattering detection. The lipids from potato tubers were used to optimize the separations. Adsorption chromatography with silica gel gave disappointing results for the plant glycolipids. Much better separations were obtained with chemically bonded stationary phases, e.g. those with propionitrile or polymeric vinyl alcohol as the functional moiety. With careful calibration, good reproducibility was possible. Only highly acidic lipids such as phosphatidylserine still present some problems.     

Lipids of Capsicum annuum Fruit Pulp

Lipids from fruit pulp of the sweet red pepper (Capsicum annuum, Solanaceae) were characterized. The principal components of the lipids from pepper fruit pulp were neutral lipids with predominance of triacylglycerines (55.8%). The polar lipids contained sulfoquinovosyldiacylglycerines and phosphotidylglycerines. The fatty-acid compositions of the neutral lipids, glycolipids, and phospholipids were determined.     

Reaction efficiency of organic alkalis with various classes of lipids during thermally assisted hydrolysis and methylation

Reaction efficiencies of two organic alkalis, tetramethylammonium hydroxide (TMAH) and trimethylsulfonium hydroxide (TMSH), with lipids during thermally assisted hydrolysis and methylation (THM) were examined focusing on (1) the types of lipids and (2) degree of unsaturation of fatty acid moieties. Different types of lipids such as triglycerides, phospholipids, free fatty acids and cholesteryl esters containing saturated, monounsaturated or polyunsaturated fatty acid (PUFA) residues were subjected to THM-gas chromatography (GC) in the presence of TMAH or TMSH. The obtained results revealed that the THM reaction using TMAH allowed almost quantitative methylation of saturated and monounsaturated fatty acid components independently of the classes of lipids. However, strong alkalinity of TMAH brought about isomerization and/or degradation of PUFA components. In contrast, the use of TMSH was effective to highly sensitive detection of PUFA as well as saturated and monounsaturated fatty acid components contained in triglycerides, phospholipids (phosphatidylcholines) and free fatty acids. On the other hand, TMSH was proved to react hardly with any kind of fatty acid residues in cholesteryl esters due to their steric hindrance.     

Lipids of cottonseed kernels

The free, bound, and strongly bound lipids of the seed kernels of the cotton plant of variety 108-F have been studied. It has been shown that the free and bound lipids include 97.3% and 47.6% of neutral lipids, respectively. The polar lipids consist mainly of phospholipids and glycolipids. Two groups of glycolipids — sterol glycosides and glycosylglycerides — have been isolated and their compositions have been determined.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 435–438, July–August, 1983.     

Lipids ofGossypium mexicanum

The surface lipids of the coat and the lipids of the kernel of the seeds of a wild form of the medium-fibered cotton plantGossypium mexicanum var.nervosum have been studied in comparison with cultured species. Using the methods of CC, TLC, and GLC, and UV and mass spectrometry, in the cuticular lipids ofG. mexicanum three classes of neutral lipids have been detected, and in the kernel lipids 10 classes of neutral lipids, 2 classes of glycolipids, and 7 classes of phospholipids. The structures of the epoxyacyl-, hydroxyacyl-, and diacylglycerols of the kernel have been established. The greatest differences between the seeds of the wild form of the cotton plant and the cultured species are the greater weight of the seed coat, the presence in it of a considerable amount of the 18:1 and of medium-molecular-weight fatty acids, and a higher level in the kernel of gossypol pigments, polar lipids, and acylglycerols containing the 18:2 acid.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 57–62, January–February, 1987.     

Fatty acid composition of neutral lipid: Classes of Citrus seed oil

The constituents of total lipids and neutral lipid classes, i.e., hydrocarbons, wax esters, sterol esters, triacylglycerols, free fatty acids, 1,3-diacylglycerols, 1,2-diacylglycerols, free sterols, alcohols, and monoacylglycerols of three species of Citrus namely Citrus sinensis, Citrus paradisi, Citrus aurantium were investigated by thin layer and gas chromatography. Palmitic, oleic and linoleic acids were the major components in all the lipids and lipid classes studied.     

Lipids of the tuberous roots ofMandragora turcomanica

The lipids of the tuberous roots ofMandragora turcomanica Mizger (fam. Solanaceae) have been characterized for the first time. By using CC, TLC, Ag⁺-TLC, UV and IR spectroscopies and mass spectrometry, 20 classes of lipids and lipophilic substances have been characterized. The main components of the acyl-containing lipids that were isolated were found to be triacylglycerols and glycolipids, and among the lipophilic compounds they were alkanols and sterols; in the mixture of fatty acids of the acyl-containing lipids the 18:2, 18:1, and 16:0 acids predominated.Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 615–623, September–October, 1994.