牛血清白蛋白-PAMAM修饰的开管毛细管电色谱柱的制备

将树枝状大分子PAMAM键合到毛细管电色谱柱中, 然后在其端基上键合配体牛血清白蛋白(BSA), 利用BSA-G3.0-PAMAM键合柱成功地分离了D,L-色氨酸.     

二烯丙基聚乙二醇20M交联键合型毛细管电泳柱的制备及其评价

本将聚乙二醇２０Ｍ改性为二烯丙基聚乙二醇２０Ｍ，使其更容易在毛细管壁进行，交联和键合，采用超动态涂柱技术，实现了快速制备二烯丙基聚乙二醇２０Ｍ交联键合型毛细管电泳柱，该制柱方法具有简单，速度快，制柱重复性高的特点，用四种碱性蛋白对所制的柱进行评价，平均柱效可达５×１０＾５理论塔板数／米，每次运行之间（Ｎ＝６），天与天之间（Ｎ＝３），以及柱与柱之间（Ｎ＝３）的迁移时间的标准偏差（ＲＳＤ％）在０．２     

毛细管电色谱柱性能的理论与应用

毛细管电色谱柱是在毛细管内充填、涂布或键合色谱固定相,用电渗流作为驱动力的一种电分离模式。总结了国外研究现状,并初步探索讨论了电色谱柱性能理论和应用指标的实现。     

开管毛细管电色谱进展

开管毛细管电色谱是近年发展起来的一种高效、快速的新型微柱分离方法。它是在毛细管管壁涂布或键合固定相,以电渗流驱动流动相的一种色谱分离模式。该文对开管毛细管电色谱的发展、柱制备、理论进行了较为详细的综述,引用文献４７篇     

辐照法制备交联聚丙烯酰胺涂层毛细管电泳柱及评价

采用辐照法制备交联聚丙烯酰胺毛细管电泳涂层柱。毛细管先经双功能团试剂γ－甲基丙烯基氧丙基三甲基硅烷预处理，然后柱内充入丙烯酰胺溶液进行辐照聚合，得到线性涂层柱；再经动态涂覆交联剂甲叉双丙烯酰胺，二次辐照交联，采用随机试验设计方法，对辐照剂量率，辐照时间和交联剂浓度几个因素进行了优化。     

以枝形大分子聚酰胺-胺(PAMAM)为键合固定相的开管毛细管电色谱柱的制备及评价

用3-氨丙基三乙氧基硅烷(KH550)作偶联剂, 在毛细管内壁上逐步合成树枝形大分子聚酰胺-胺(PAMAM), 制得了1, 2和3代PAMAM键合的开管毛细管电色谱柱, 并对其性能进行了研究. 结果表明, 随着大分子代数的增加, 毛细管电渗流(EOF)逐步下降. 利用制得的1, 2和3代PAMAM修饰的开管毛细管电色谱柱对丙氨酸和脯氨酸的分离进行对比, 结果显示, 随着大分子PAMAM代数的增加, 分离度逐步增大, 丙氨酸和脯氨酸可在3代树枝状大分子PAMAM修饰的开管毛细管电色谱柱上达到基线分离. 采用非衍生化法和3代PAMAM修饰的开管毛细管电色谱柱成功地分析了精氨酸、 丙氨酸、 脯氨酸、 甲硫氨酸和组氨酸. 结果表明, 键合毛细管柱具有良好的重现性和稳定性.     

3-氨丙基键合开管柱电色谱分离中性芳香族化合物

制备了3-氨丙基键合毛细管开管柱,在此柱上以氨水/甲醇(体积比30∶70)溶液为溶剂,采用毛细管电泳法有效地分离了八种中性芳香族化合物.实验中考察了电色谱条件如:氨水浓度、表面活性剂十二烷基硫酸钠(SDS)浓度及电压等对分离度的影响.结果表明八种中性芳香族化合物在该柱上的分离效果和重现性良好.     

交联聚丙烯酰胺毛细管电泳涂层柱的制备及其应用

摘要建立了一种简便的制备交联聚丙烯酰胺型毛细管电泳涂层柱的方法. 所制备的涂层柱能够有效地抑制电渗流及蛋白质在管壁上的吸附. 考察了碱性蛋白质在pH=4。0的缓冲溶液中的分离, 迁移时间重现性误差小于2。1%. 对麻黄提取物中的生物碱进行了分离, 平均理论塔板数为24×105 plates/m.     

羧甲基壳聚糖修饰的开管毛细管电色谱柱的制备与表征

以γ-(2,3-环氧丙氧基)丙基三甲氧基硅烷为偶联剂,将羧甲基壳聚糖键合至毛细管内壁上。对毛细管预处理、硅烷化以及羧甲基壳聚糖键合反应参数进行了优化,考察了开管柱内表面结构形貌、电渗流、重现性和电色谱分离性能。扫描电镜图显示羧甲基壳聚糖均匀地键合在毛细管内表面。调节流动相的pH值可产生正向和反向电渗流,电渗流的相对标准偏差(RSD): 日内小于0.8%(n=6)、日间小于3.5%(n=3)、同一批次间小于4.3%(n=3)、不同批次柱间小于6.1%(n=3),重现性较好。4种核苷酸(腺嘌呤核苷酸、鸟嘌呤核苷酸、胞嘧啶核苷酸、尿嘧啶核苷酸)在修饰后的毛细管电色谱柱上得到了较好的分离,柱效达到36000～182000 塔板/m。结果表明,所建立的开管柱制备方法简单有效、稳定性好。     

牛血清白蛋白修饰毛细管整体柱的制备及组氨酸对映体分离

以甲基丙烯酸缩水甘油酯为单体, 乙二醇二甲基丙烯酸酯为交联剂, 环己醇和正十二醇混合溶液为致孔剂, 在最佳聚合条件下, 以偶氮二异丁腈为引发剂, 制备了毛细管整体柱基质, 并且研究了单体、交联剂及致孔剂对整体柱基质孔结构及渗透性的影响; 使用Epoxy方法在基质表面键合BSA, 制得BSA修饰的毛细管整体柱. 将此毛细管整体柱应用于毛细管电色谱中, 成功地分离出了组氨酸对映体, 分离度良好.     

多环芳二酐型聚酯亚胺膜的透气性能

多环芳二酐型聚酯亚胺膜的透气性能李悦生，丁孟贤，徐纪平（浙江大学高分子科学与工程研究所，杭州，３１００２７）（中国科学院长春应用化学研究所）关键词聚醚酰亚胺，聚酯酰亚胺，膜，透气性通常的聚酰亚胺加工性能较差，在芳环二酐的苯环间引入醚键等柔性基团后，其...     

微量元素与疾病诊断治疗的研究现状及展望

对国内外微量元素与疾病的关系及其诊断治疗的研究现状及研究动向进行了综述，包括：微量元素与地方病、癌肿、心血管病、糖尿病、眼病、神经系统病等。参考文献72篇。     

Determination and study on residue and dissipation of benazolin-ethyl and quizalofop-p-ethyl in rape and soil

A QuEChERS (quick, easy, cheap, effective, rugged, and safe) method for the determination of benazolin-ethyl and quizalofop-p-ethyl in rape and soil by high-performance liquid chromatography-tandem mass spectrometry has been developed in this study. The residue and dissipation of benazolin-ethyl and quizalofop-p-ethyl in rape and soil were determined with the developed method. The half-lives of benazolin-ethyl in rape straw and soil were 3.7–5.1 days and 14.3–26.3 days, respectively. The half-lives of quizalofop-p-ethyl in rape straw and soil were 5.0-6.1 days and 0.3–9.7 days, respectively. The residue of benazolin-ethyl and quizalofop-p-ethyl in rapeseed and soil were below the detection limit (i.e., 0.5?mg?kg^?1, the maximum residue level of European Union for quizalofop-p-ethyl).     

Ferrocene and ferrocenium modified clays and their styrene and EVA composites

In this work, ferrocene- and ferrocenium-containing salts were employed to modify montmorillonite. X-ray measurements show an increase in the interlayer spacing upon clay modification, which means that the larger and more organophilic cations were inserted into the gallery space of montmorillonite. Attempts to prepare nanocomposites of polystyrene and ethylene vinyl acetate copolymers lead to immiscible systems; the morphology of these systems was elucidated with TEM, XRD and cone calorimetry. The thermal stability of the composites is greater than that of the virgin polymer.     

Synthesis and Optical and Redox Properties of Symmetric and Asymmetric BODIPYs

Herein, we present the synthetic route and the photophysical, electrochemical as well as laser properties of novel red‐emitting boron‐dipyrromethenes (BODIPYs) bearing arylethyne moieties. Such functionality is added along the main axis of the chromophore leading to single‐ and double‐substituted derivatives. The relationship between the dye structure and the lasing properties is studied in detail with the help of the photophysical and electrochemical properties as well as quantum mechanical simulations. The asymmetric substitution of the parent dye induces inhomogeneities in the charge distribution, which leads to an overall loss of the fluorescence capacity, mainly in polar media. Such non‐radiative deactivation processes can be softened by decreasing the electron‐donor ability of the substituent or even avoided by symmetrical substitution. Thus, grafting of the arylethyne moieties at the longitudinal axis of the indacene core results in an effective strategy to develop red‐edge BODIPYs with highly efficient and photostable laser emission.     

Conformations and steric and stacking interactions of trisubstituted ureas and thioureas with alkyl and phenyl groups

The monomeric ν(N—H) vibrations of various trisubstituted ureas of the R₂UPh type and -thioureas of the R₂TUPh type have been studied. The trans—out isomerism in the former and the trans—out—cis isomerism in the latter are discussed from the point of view of steric effect. The monomeric ν(N—H) vibrations of RPhUPh and RPhTUPh are also examined. The single band appearing in the spectrum of both ureas is characteristic of the cis form; this suggests the existence of phenyl—phenyl interaction (the stacking interaction proposed by Galabov et al. [10]). Behavior of ν(N—H) vibrations at several concentrations is shown to be clearly different in the three forms (trans, out and cis). The presence of the cis form is confirmed by solvent effect experiments.     

Ti-V基储氢合金及其氢化物的物相结构与组分优化设计

Ti-V基储氢合金在室温、常压下即可表现出良好的储氢特性,且质量储氢容量明显高于传统AB₅型储氢合金,从而在氢气的精制和回收、运输和储存及热泵等方面有较早的应用。 此外,在混合气体分离、核反应堆中处理氢的同位素、镍氢电池及燃料电池负极材料等方面也得到了广泛的研究与关注。 基于目前Ti-V基储氢合金的研究现状,概述了该类合金的优势、限制性因素(包括成因)及改性手段。 此外,为了进一步理解Ti-V基合金储氢机理、构建合金组分与储氢特性之间的对应关系,本工作重点围绕Ti-V基储氢合金及其氢化物的结构、组分优化设计展开综述,并对其未来研究方向做出展望。     

钒与糖代谢及糖尿病

微量元素钒作为生物体内必需元素，经证实有很重要的生理学功能，与机体糖代谢有十分密切的关系，且能降低糖尿病动物模型的高血糖，极有可能被开发为治疗糖尿病的药物。     

Homo- and copolymerizaton of norbornene and norbornene derivative with Ni- and Pd-based β-ketoiminato complexes and MAO

Neutral nickel(II) and palladium(II) complexes bearing β-ketoiminato ligand have been synthesized. The two complexes have been investigated as catalyst for the polymerization. Using methylaluminoxane (MAO) as a cocatalyst, both complexes produce vinyl-addition polynorbornenes, but palladium(II) complex displays much higher activity up to 8.0 × 10⁷ g/(molPd h). Furthermore, both Ni(II) and Pd(II)/MAO system can efficiently copolymerize norbornene and 5-norbornene-2-yl acetate (NB-OCOMe) in moderate yields and in relatively high molecular weights. The analyses of the product by FTIR, ¹H NMR and ¹³C NMR spectra give the verification of vinyl addition copolymer. The copolymers show narrow molecular weight distribution and good solubility in common organic solvents.