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1.
卷烟烟气中挥发性组分的毛细管气相色谱分析   总被引:11,自引:1,他引:10  
采用毛细管气相色谱(CGC)和色谱-质谱联用(GC-MS)技术对国内外部分名牌卷烟烟气中的挥发性和半挥发性化学成分进行了定性定量分析,质谱鉴定出107种化合物;对其中重要的32种化合物进行了定量比较,结果发现烤烟型卷烟、混合型卷烟和英式烤烟在烟气的香味组分中存在一些差异和规律,烤烟型烟气组分总含量最高,烤烟烟气中碳氢化合物裂解产物和低级酸类组分含量较高,混合型卷烟烟气中茄酮和异戊酸较高,英式烤烟的烟气组分更接近于混合型卷烟。  相似文献   

2.
啤酒花酊挥发性香味成分的GC-MS分析   总被引:4,自引:0,他引:4  
利用连续液-液提取技术分离啤酒花酊的香味成分,用GC-MS和双柱复检法对啤酒花酊的挥发性化学成分进行了定性分析,质谱共鉴定出44种化合物,其主成分为4,4-dimithy1-3,5-dioxahexaldoxin-2,6-dione和3-甲基丁酸。对主要成分进行定量和重现性比较,结果令人满意。该法简便、快速,为食品工业添加剂的质量监控和常规分析提供参考。  相似文献   

3.
采用超高效液相色谱-电喷雾串联质谱法(UPLC-ESI MS/MS)并结合气相色谱-质谱法分离鉴定了烟草中5种主要的糖苷类香味前体物质。烟样经甲醇提取、XAD-2柱净化,得到初步纯化的糖苷,在pH 5条件下将其酶解,释放出糖苷配基。采用气相色谱-质谱分析并通过标准谱库检索确定了5种挥发性苷元;然后通过电喷雾质谱(负离子模式)确定糖苷母离子并作碎片离子扫描(MS2),确定了5种糖苷类香味前体物质的存在形式;最后采用UPLC-ESI MS/MS,以甲醇和乙酸-乙酸铵水溶液为流动相,通过RP-C18柱分离,在多反应监测(MRM)模式下,鉴定了烟草中5种主要的糖苷类香味前体物质,为应用液相色谱-质谱分析缺乏标准样品的糖苷类香味前体物质奠定了基础。  相似文献   

4.
水蒸气蒸馏提取与顶空进样GC-MS分析高良姜挥发性成分   总被引:1,自引:0,他引:1  
比较了水蒸气蒸馏法和顶空加热法提取高良姜挥发性成分的方法,并进行GC-MS分析。结果表明,两种提取方法无论在成分还是其相对含量均存在一定的差异。采用顶空进样技术可鉴定出31个挥发性化学成分,采用水蒸气蒸馏法可鉴定出31个挥发性化学成分,两种方法共有成分为16种。两种样品采集方法分别提供了高良姜不同沸点挥发物的化学信息,运用这两种方法可以建立更全面的高良姜挥发性成分GC-MS表征体系。  相似文献   

5.
运用静态顶空气相色谱-质谱法对22个胶基型嚼烟样品中的挥发性香味物质进行分析,并采用质谱数据库对挥发性香味物质进行定性分析。对顶空分析条件以及GC-MS条件进行了优化,提高了挥发性香味物质的测定灵敏度;对峰面积归一化结果大于0.1%的化合物进行定性分析,通过匹配度限定(≥85%)确认了65种挥发性香味物质;最后对样品进行了相似性分析和聚类分析。结果表明:22个胶基型嚼烟样品中的香味物质主要有酯类(18个化合物)、烯烃类(16个化合物)、醇类(9个化合物)、醛类(8个化合物)、酮类(7个化合物)、酚类(1个化合物)、酸类(1个化合物)、呋喃类(2个化合物)、烷烃类(2个化合物)、硫醚类(1个化合物),共10类化合物;不同胶基型嚼烟样品的品牌识别度高,同时口味对品牌识别有一定的影响作用。方法为进一步开发胶基型嚼烟制品提供了理论基础和技术支持。  相似文献   

6.
冠心苏合丸中挥发性组分的分析及主成分冰片的测定   总被引:1,自引:0,他引:1  
采用同时蒸馏-萃取法提取冠心苏合丸中的挥发性组分,测得两种冠心苏合丸中的挥发性组分含量分别为14%和9.8%。利用GC-MS方法对两种冠心苏合丸所得的挥发性组分进行分析,通过检索NIST98谱图库,并结合标准谱图库和有关文献,从冠心苏合丸的挥发性组分中分别确定出14、19种化学成分,分别占挥发性组分总检出量的97.24%和98.76%。用峰面积归一化法,得出各化学成分在挥发性组分中的相对百分含量。主要挥发性组分是冰片。并采用气相色谱法对冠心苏合丸中冰片的含量进行了测定冰片质量浓度在1.0~5、0mg/mL范围内成良好的线性关系,冰片的平均回收率为98%~106%。  相似文献   

7.
采用内部萃取电喷雾电离质谱(iEESI-MS)技术,以甲醇作为萃取溶剂,无需样品预处理,在优化条件下分别对牛油果果肉和果皮中化学成分进行快速直接鉴定,并考察了不同成熟度牛油果果肉中化学成分的差异.实验结果表明,在负离子检测模式下,从果肉中鉴定出棕榈酸、硬脂酸、棕榈油酸、油酸和亚麻酸等12种有效营养成分,从果皮中鉴定出儿茶素、绿原酸等11种营养成分.利用正交偏最小二乘判别分析法(OPLS-DA)对不同成熟度的牛油果果肉的iEESI-MS指纹谱图数据进行分析发现,此方法能够有效判别不同成熟程度的牛油果且5种化学成分差异显著.本方法无需样品预处理、样品耗量少、分析速度快(单个样品检测时间小于1 min)且操作简便,为植物有效营养成分和医用价值的开发提供了一种快速质谱分析新方法.  相似文献   

8.
提出了非极性溶剂微波萃取-气相色谱-质谱法测定白豆蔻中的挥发油成分的方法。优化的试验条件如下:①微波吸收介质为0.35g石墨粉;②提取溶剂为正己烷;③样品质量与溶剂容积之比为2g比25mL;④提取时间为30min。在气相色谱分离中用DB-5石英毛细管柱为固定相,在质谱分析中采用全扫描检测模式。以α-甲基苯甲醇丙酸酯为内标物。方法用于白豆蔻样品的分析,共鉴定出60种挥发性化学成分,主要化合物为桉油精(70.34%)、β-蒎烯(6.81%)、α-松油醇(3.36%)和α-蒎烯(2.54%)等。  相似文献   

9.
提出了基于低密度溶剂超声辅助-表面活性剂增强乳化微萃取-气相色谱-质谱法测定薄荷芳香水中的挥发性成分。优化的试验条件如下:1萃取剂为甲苯;21mg·L-1吐温80溶液的用量为30μL;3超声时间为1min。在气相色谱分离中用DB-5石英毛细管柱为固定相,在质谱分析中采用全扫描检测模式。方法用于薄荷样品的分析,薄荷芳香水中共鉴定出20种挥发性成分,薄荷挥发油中共鉴定出30种挥发性成分,其中所含的主要成分基本一致。  相似文献   

10.
建立了基于傅立叶变换离子回旋共振超高分辨质谱(FTICR-MS)的赤灵芝化学成分鉴定和指纹图谱分析方法,并应用于不同产地赤灵芝样品的来源区分。样品采用50%甲醇进行回流提取后,以流动注射的进样方式进行直接质谱分析。以ESI离子源在负离子模式下进行检测,质荷比扫描范围为100~1 000 Da。采用精确分子量测定和碰撞诱导解离实验进行化学成分鉴定,通过与文献进行比对,共鉴定出63种化学成分(1种萜烯醛、3种糖、4种三萜醇、6种有机酸和49种三萜酸类成分)。采用聚类分析和主成分分析(PCA)对所获得的质谱指纹图谱进行统计学分析,在95%的置信区间下,多个批次不同产地的赤灵芝样品得到了较好的来源区分。研究结果表明该方法可实现赤灵芝的直接、快速、高效分析和指纹图谱研究,在中药分析领域有着广阔的应用前景。  相似文献   

11.
气相色谱-质谱测定樟林番荔枝种子挥发油的脂肪酸组成   总被引:1,自引:0,他引:1  
用溶剂萃取法提取樟林番荔枝果实种子中的挥发性物质,测定出其挥发油质量分数为13.3%;利用GC-MS方法分离确认出其中的9种化学成分;用面积归一化法得出了9种脂肪酸在挥发油中的质量分数;其中9-十八烯酸占49.42%,十六酸占20.37%,十八酸占14.16%,9,2-十八二烯酸占13.59%;不饱和脂肪酸,占63.01%.该项研究给番荔枝果实的深入开发利用及种质资源的有效保护提供了科学依据.  相似文献   

12.
Shexiang Xintongning tablet (SXXTN) is a traditional Chinese medicine (TCM) preparation for the treatment of coronary heart disease (CHD) angina pectoris. However, due to the complexity of the compounds in SXXTN, the active chemical components responsible for the therapeutic effect are still ambiguous. The purpose of our study was to characterize the chemical profile of SXXTN and quantify the representative chemicals. The high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-QTOF MS) method and gas chromatograph coupled with mass spectrometry (GC–MS) method were utilized to identify the chemical constituents of SXXTN. A total of 140 compounds including alkaloids, ginsenosides, organic acids, esters, triterpenes, phthalides and amino acid were identified in accordance with their retention times, accurate masses and characteristic MS/MS fragment patterns. Forty-four volatile components were characterized by GC–MS through NIST database matching. In the further research of quantitative analysis, 40 non-volatile compounds and 17 volatile compounds were determined and successfully applied for detecting in 7 batches of SXXTN samples by high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HPLC-QQQ MS) and gas chromatograph coupled with triple-quadrupole tandem mass spectrometry (GC-QQQ MS) in multiple reaction monitoring (MRM) mode, respectively. The quantitative methods were verified in linearity, precision, repeatability stability and recovery. The above results indicated that the established method was practical and reliable for synthetical quality evaluation of SXXTN. In addition, our study might supplement the chemical evidence for disclosing the material basis of its therapeutic effects.  相似文献   

13.
顶空固相微萃取-气相色谱-质谱分析发酵香肠挥发性成分   总被引:9,自引:0,他引:9  
采用固相微萃取-气相色谱-质谱(SPME-GC-MS)联用分析技术对发酵香肠挥发性成分进行了分析。共鉴定了33种化合物。结果表明,发酵香肠的挥发性成分主要为醇类、酮类、酯类、有机酸类、酚类及醛类等。  相似文献   

14.
Qiu Y  Lu X  Pang T  Ma C  Li X  Xu G 《Journal of separation science》2008,31(19):3451-3457
Comprehensive 2-D GC (GC x GC) coupled with TOF MS or flame ionization detector (FID) was employed to characterize and quantify the chemical composition of volatile oil in the radixes of Panax ginseng C. A. Mey. (ginseng) at different ages. Thirty-six terpenoids were tentatively identified based on the MS library search and retention index in a ginseng sample at the age of 3 years. An obvious group-type separation was obtained in the GC x GC-TOF MS chromatogram. The data collected by GC x GC-FID were processed using a principal component analysis (PCA) method to classify the samples at different ages. The compounds responsible for the significant differentiation among samples were defined. It was found that the relative abundances of alpha-cadinol, alpha-bisabolol, thujopsene, and n-hexadecanoic acid significantly rise with the increase in age.  相似文献   

15.
Two different analytical approaches, direct exposure electron ionization mass spectrometry (DE-MS) and gas chromatography/mass spectrometry (GC/MS), were compared in a study of archaeological resinous materials. DE-MS was found to be an efficient fingerprinting tool for the fast screening of organic archaeological samples and for providing information on the major components. GC/MS appeared to be more efficient in unravelling the sample composition at a molecular level, despite the long analysis time and the need for a wet chemical pretreatment. Both procedures were applied to characterize the organic material present as coatings in Roman and Egyptian amphorae. DE-MS successfully identified abietanic compounds, hence a diterpenic resinous material could be identified and its degree of oxidation assessed. GC/MS enabled us to identify dehydroabietic acid, 7-oxodehydroabietic acid, 15-hydroxy-7-oxodehydroabietic acid, 15-hydroxydehydroabietic acid, retene, tetrahydroretene, norabietatriene, norabietatetraene and methyl dehydroabietate. These oxidized and aromatized abietanes provided evidence that the amphorae examined were waterproofed with a pitch produced from resinous wood of plants from the Pinaceae family. The chemometric evaluation of the GC/MS data highlighted significant chemical differences between the pitches found in the two archaeological sites, basically related to differences in the production techniques of the materials and in their degradation pathways.  相似文献   

16.
A method using gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS) and (1)H NMR with pattern recognition tools such as principle components analysis (PCA) was used to study the human urinary metabolic profiles after the intake of green tea. From the normalized peak areas obtained from GC/MS and LC/MS and peak heights from (1)H NMR, statistical analyses were used in the identification of potential biomarkers. Metabolic profiling by GC/MS provided a different set of quantitative signatures of metabolites that can be used to characterize the molecular changes in human urine samples. A comparison of normalized metabonomics data for selected metabolites in human urine samples in the presence of potential overlapping peaks after tea ingestion from LC/MS and (1)H NMR showed the reliability of the current approach and method of normalization. The close agreements of LC/MS with (1)H NMR data showed that the effects of ion suppression in LC/MS for early eluting metabolites were not significant. Concurrently, the specificity of detecting the stated metabolites by (1)H NMR and LC/MS was demonstrated. Our data showed that a number of metabolites involved in glucose metabolism, citric acid cycle and amino acid metabolism were affected immediately after the intake of green tea. The proposed approach provided a more comprehensive picture of the metabolic changes after intake of green tea in human urine. The multiple analytical approach together with pattern recognition tools is a useful platform to study metabolic profiles after ingestion of botanicals and medicinal plants.  相似文献   

17.
酸枣果肉中挥发性化学成分的提取及分析   总被引:7,自引:2,他引:5  
报道了用蒸馏-萃取法和同时蒸馏-萃取法提取酸枣果肉中挥发性物质,测得两种方法提取的酸枣果肉挥发油含量分别为1.80%和2.30%。用GC/MS法从酸枣果肉挥发油中分别分离并确定出43种和28种化学成分,分别占酸枣果肉挥发油总检出量的95.62%和94.88%。用峰面积归一化法通过化学工作站数据处理系统,得出各化学成分在挥发油中的相对百分含量。  相似文献   

18.
The volatile components of yak butter were isolated by solvent-assisted flavour evaporation (SAFE), simultaneous distillation extraction (SDE; dichloromethane and diethyl ether as solvent, respectively) and headspace solid-phase microextraction (HS-SPME; CAR/PDMS, PDMS/DVB and DVB/CAR/PDMS fibre extraction, respectively) and were analysed by GC/MS. A total of 83 volatile components were identified under six different conditions, including 28 acids, 12 esters, 11 ketones, 10 lactones, 10 alcohols, 4 other compounds, 2 aldehydes, 2 unsaturated aldehydes, 1 furan, 1 sulphur-containing compound, 1 unsaturated alcohol and 1 unsatruated ketone. Among them, 51 were identified by SAFE, 58 by SDE (45 with dichloromethane as solvent and 41 with diethyl ether as solvent) and 40 by HS-SPME (26 with CAR/PDMS; 26 with PDMS/DVB and 32 with DVB/CAR/PDMS). Three pretreatment methods were compared to show that the volatile components obtained using different methods varied greatly, both in terms of categories and in content. Therefore, a multi-pretreatment method should be adopted, together with GC/MS. A total of 25 aroma-active compounds were detected by gas chromatography-olfactometry, among which 20 aroma-active compounds were found by SDE (14 with dichloromethane as solvent and 14 with diethyl ether as solvent) and 17 by SAFE.  相似文献   

19.
The volatile compounds of γ-irradiated dried Welsh onion were isolated by simultaneous distillation–extraction (SDE) technique and then analyzed by gas chromatography–mass spectrometry (GC–MS) along with their non-irradiated counterparts. A total of 35 volatile compounds were identified in non-irradiated and 1 kGy irradiated samples and 36 volatile compounds were identified in 3, 5, 10 and 20 kGy irradiated samples so far belong to chemical classes of acid, alcohol, aldehyde, ester, furan, ketone and S-containing compound. S-containing compounds were detected as major volatile compounds of all experimental samples. Though the content of several compounds was increased after irradiation, content of major S-containing compounds was found to decreased in the process. Application of high-dose irradiation if required for microbial decontamination of dried Welsh onion is feasible as it enhanced the total concentration of volatile compounds by 31.60% and 24.85% at 10 and 20 kGy, respectively.  相似文献   

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