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荧光关联谱方法凭借其独特的对溶质浓度及扩散系数的测量能力,在对复杂系统的分子物理化学性质测量方面,不断扩大和深化其应用领域。但是从经典上,传统的荧光关联谱数据处理方法容易在拟合自相关曲线的参数时引入一定的误差,而且图表上的直观信息不多。针对以上问题,在文章中作者尝试用带有微分的数据处理办法,来更直观地估测参数并判断荧光多组分问题。经典自相关曲线处理后的微分曲线,有特异的波谷位置、深度和半深宽度,由此可判断特征扩散时间和多荧光成分的组分数目。此改进方法为利用荧光涨落谱方法测量生物组织体内的复杂环境提供帮助。 相似文献
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梯度场中荧光关联谱测量的Monte Carlo模拟 总被引:1,自引:1,他引:0
荧光关联谱(FCS)利用少量分子的荧光涨落获得分子运动和反应信息,是一种探测活细胞内生物过程的有力工具。本文利用Monte Carlo方法模拟激光梯度场下瑞利粒子的布郎运动,分析梯度场对FCS测量得到的扩散时间、粒子数等参数的影响,模拟与初步实验结果定性吻合。文中还讨论了Monte Carlo模拟预测的一些实验现象。 相似文献
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《光散射学报》2015,(3):305-310
采用二维荧光相关光谱技术有效地对严重重叠的蒽、芘混合溶液的荧光峰进行解析。为了实现上述研究目标,设计并配置了9个蒽、芘混合溶液。采集了蒽、芘单组份以及两种物质混合溶液的常规一维荧光谱,研究了其荧光特性,并进行对比、分析。在此基础上,以混合溶液中蒽和芘的浓度为外扰,构建二维荧光相关同步谱和异步谱。结果表明:在同步谱上出现6个较强的自相关峰,位置分别在373nm、379nm、391nm、401nm、413nm和425nm处;依据未被覆盖的蒽在425nm的处荧光与各波长处荧光交叉峰的正负,指出379nm、401nm和452nm处的荧光峰来自蒽,而373nm、391nm和413nm处的荧光峰来自芘。同时,又根据相关异步谱交叉峰的有无,进一步确认和验证了混合溶液中各荧光峰的来源。该方法为解析农业环境中多种污染物的光谱特征提供理论和实验基础。 相似文献
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《物理学报》2017,(1)
自旋噪声谱是一种非扰动的自旋动力学研究方法,通过探测系统在非激发条件下的自旋涨落,可以揭示系统在热平衡状态下的性质.因为系统在稳态下的自旋涨落十分微弱,所以提高信噪比在自旋噪声谱的测量中特别重要.本文采用频谱仪、数据采集卡和实时傅里叶变换采集卡三种方法来测量铷原子气体的自旋噪声谱,并将实验结果进行对比,分析了叠加次数、测量效率和采样深度等因素对谱线信噪比的影响.实验发现,谱线叠加次数对自旋噪声谱的信噪比影响最为显著,测量效率则能反映不同方法在相同的测量时间内得到的谱线质量,并比较了三种方法的测量效率,采样深度的提高并不能明显改善自旋噪声谱的信噪比.相比于传统的频谱仪和数据采集卡,实时傅里叶变换采集卡的数据利用率和测量效率更高,从而具有更好的信噪比,非常有利于自旋噪声谱在自旋动力学研究中的应用. 相似文献
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石油类混合油液的组分检测是三维荧光光谱领域重要的研究内容,由于实际获得的混合油液三维荧光光谱数据存在不同组分光谱重叠严重、数据三线性较差等问题,通过平行因子算法解析时,会出现解析谱与标准谱差异过大或者不能正确判断油种的情况。在验证三维荧光偏导数光谱应用平行因子算法具有可行性的基础上,将三维荧光偏导数光谱与平行因子算法结合,能够提高平行因子算法得到的混合油解析谱与标准谱的拟合程度,实现石油类混合油液组分的准确检测。首先,以十二烷基硫酸钠(SDS)溶液作为溶剂,配制航空煤油、润滑油不同浓度的纯油溶液各15份,将航空煤油、润滑油按照不同浓度比配制9份混合油溶液;并利用FS920荧光光谱仪得到39份三维荧光光谱数据。然后,对三维荧光光谱数据进行预处理:通过扣除空白法去除拉曼散射,并将瑞利散射区域扣除,再利用分段三次hermite插值方法对扣除区域进行插值;利用小波变换阈值去噪法去除光谱数据中的高频噪声,得到预处理完成后的三维荧光光谱数据。最后,利用Savitzky-Golay拟合求导方法求三维荧光光谱的一阶偏导数光谱,并利用平行因子算法对三维荧光光谱和三维荧光偏导数光谱进行解析。将解析谱与纯油标准谱进行比较,实验结果表明:利用平行因子算法对混合油液的三维荧光光谱进行解析时,得到的润滑油解析结果较好,但航空煤油的解析结果存在较大问题。而三维荧光偏导数光谱经平行因子算法解析后,在保证润滑油解析结果的同时,显著提高了航空煤油的解析结果:航空煤油解析谱与标准谱之间的相关系数提升了12.0%(发射光谱)、6.7%(激发光谱),均方根误差减少了70.4%(发射光谱)、20.6%(激发光谱)。在三维荧光光谱数据三线性较差的情况下,三维荧光偏导数光谱结合平行因子分析方法优于三维荧光光谱结合平行因子分析方法,实现了对混合油液组分准确检测的目的。 相似文献
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荧光内滤效应以及样品池中荧光物质对激发光的吸收分布会直接影响荧光谱的强度和谱形,共同制约荧光分析法的应用。使用吸收谱及荧光谱存在交迭的双组分混合溶液,研究一种新的基于物理吸收模型的校正方法,以校正荧光内滤效应及吸收分布对荧光强度的影响。对三聚噻吩和五聚噻吩混合液的光谱研究表明,使用所述校正方法可以达到较为理想的校正效果,误差小于5%。 相似文献
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M. Kohl J. Neukammer U. Sukowski H. Rinneberg D. Wöhrle H.-J. Sinn E. A. Friedrich 《Applied physics. B, Lasers and optics》1993,56(3):131-138
We report on a technique to improve fluorescence images of superficially growing tumors marked with photosensitizers. Exploiting the longer fluorescence decay times of porphyrin-based photosensitizers compared to average decay times of tissue autofluorescence, delayed detection of laser-induced fluorescence allows to suppress the autofluorescence background. The feasibility of delayed fluorescence imaging of tumors has been demonstrated in-vitro. It follows from an analysis of delayed fluorescence spectra that autofluorescence background falling into the photosensitizer fluorescence band can be reduced by up to one order of magnitude. 相似文献
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We built a time-resolved confocal fluorescence spectroscopy system equipped with the multichannel time-correlated single-photon-counting technique. The instrument provides a unique approach to study the fluorescence sensing of cell metabolism via analysis of the wavelength- and time-resolved intracellular autofluorescence. The experiments on monolayered cell cultures show that with UV excitation at 365 nm the time-resolved autofluorescence decays, dominated by free-bound reduced nicotinamide adenine dinucleotide signals, are sensitive indicators for cell metabolism. However, the sensitivity decreases with the increase of excitation wavelength possibly due to the interference from free-bound flavin adenine dinucleotide fluorescence. The results demonstrate that time-resolved autofluorescence can be potentially used as an important contrast mechanism to detect epithelial precancer. 相似文献
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应用荧光光谱技术研究溶液中血卟啉单甲醚(HMME)的光漂白与光产物生成.以532 nm倍频Nd:YAG激光器照射样品,功率密度为100 mW·cm-2,以光学多通道分析仪(OMA)采集荧光光谱.照光过程与荧光光谱采集同步进行.通过构建基本光谱与最小二乘拟合,由单条实测光谱中分解求得HMME荧光(613 nm)、光产物荧光(639 nm)及自体荧光的强度.HMME初始浓度不超过10μg·mL-1时符合荧光-浓度线性函数关系.对照光过程的荧光光谱监测同时观察到HMME漂白、光产物生成与漂白,以及样品光学特性变化引起的自体荧光强度起伏.光产物漂白后的二次产物引起样品光学特性显著改变.所建立的荧光光谱探测系统与光谱分析方法可满足光敏剂漂白特性体外研究的需要,并为光动力治疗的剂量学在体监测提供有效研究方法. 相似文献
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荧光光谱及其成像技术在光活检中的应用 总被引:11,自引:5,他引:6
分析了人体组织自体荧光的主要物质来源和荧光特性,同时总结了国内外常见的第二代新型光敏剂及其临床应用情况。在此基础上,全面比较了各种应用于早期肿瘤光活检的荧光光谱及其成像技术,并重点讨论了这些技术的基本原理和临床应用现状,以及它们今后的发展方向。 相似文献
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Flávia?Rodrigues?de Oliveira Silva Maria?Helena?Bellini Vivian?Regina?Trist?o Nestor?Schor Nilson?Dias?VieiraJr. Lilia?Coronato?Courrol 《Journal of fluorescence》2010,20(6):1159-1165
Prostate cancer is one of the most common types of cancer in men, and unfortunately many prostate tumours remain asymptomatic
until they reach advanced stages. Diagnosis is typically performed through Prostate-Specific Antigen (PSA) quantification,
Digital Rectal Examination (DRE) and Transrectal Ultrasonography (TU). The antigen (PSA) is secreted by all prostatic epithelial
cells and not exclusively by cancerous ones, so its concentration also increases in the presence of other prostatic diseases.
DRE and TU are not reliable for early detection, when histological analysis of prostate tissue obtained from a biopsy is necessary.
In this context, fluorescence techniques are very important for the diagnosis of cancer. In this paper we explore the potential
of using endogenous phorphyrin blood fluorescence as tumour marker for prostate cancer. Substances such as porphyrin derivatives
accumulate substantially more in tumours than in normal tissues; thus, measuring blood porphyrin concentration by autofluorescence
intensity may provide a good parameter for determining tumour stage. In this study, the autofluorescence of blood porphyrin
was analyzed using fluorescence and excitation spectroscopy on healthy male NUDE mice and in those with prostate cancer induced
by inoculation of DU145 cells. A significant contrast between the blood of normal and cancer subjects could be established.
Blood porphyrin fluorophore showed an enhancement on the fluorescence band around 632 nm following tumour growth. Fluorescence
detection has advantages over other light-based investigation methods: high sensitivity, high speed and safety. However it
does carry the drawback of low specificity of detection. The extraction of blood porphyrin using acetone can solve this problem,
since optical excitation of further molecular species can be excluded, and light scattering from blood samples is negligible. 相似文献
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Flávia R. O. Silva Camila T. Nabeshima Maria H. Bellini Nestor Schor Nilson D. Vieira Jr Lilia C. Courrol 《Journal of fluorescence》2013,23(1):131-135
This paper describes the elimination of porphyrins by feces. It was demonstrated that porphyrin accumulates substantially more in tumors than in normal tissues, and consequently more PPIX reaches the blood of patients and animals with tumors, and then, it needs to be eliminated. The fluorescence of feces revealed that there are large amounts of PPIX in the excreta of animals with cancer comparing with healthy animals. The autofluorescence of feces porphyrin extracted with acetone was analyzed using fluorescence spectroscopy of animals inoculated with DU145 cells into the prostate and healthy animals to monitor the PPIX concentration. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences were observed in autofluorescence intensities measured in the 575–725 nm spectral regions for the studied groups. The results showed a noninvasive, simple, rapid and sensitive method to detect cancer by feces analysis. 相似文献
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We present a simulation model for a stream of photon counts that can be detected in fluorescence fluctuation spectroscopy
systems from diffusing molecules undergoing spontaneous transitions between radiative and nonradiative states. Using our model,
we can obtain various characteristics of a random stream of photon counts which allows us to test fluorescence fluctuation
spectroscopy methods and to predict the results of full-scale experiments. 相似文献
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Courrol LC de Oliveira Silva FR Coutinho EL Piccoli MF Mansano RD Vieira Júnior ND Schor N Bellini MH 《Journal of fluorescence》2007,17(3):289-292
Renal cell carcinoma (RCC) accounts for approximately 3% of new cancer incidence and mortality in the United States. Unfortunately
many RCC masses remain asymptomatic and nonpalpable until they are advanced. Diagnosis and localization of early carcinoma
play an important role in the prevention and curative treatment of RCC. The autofluorescence of blood porphyrin of healthy
and tumor induced in male SCID mice was analyzed using fluorescence and excitation spectroscopy. A significant contrast between
normal and tumor blood could be established. Blood porphyrin fluorophore showed enhanced fluorescence band (around 630 nm)
in function of the tumor growth. This indicates that either the autofluorescence intensity of the blood fluorescence may provide
a good parameter for the “first approximation” characterization of the tumor stage. 相似文献
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MENG Fanbo CHEN Bo LIU Guang DING Jianying & MA Hui . Department of Physics Key Laboratory of Atomic Molecular Nano Sciences Ministry of Educa- tion Tsinghua University Beijing China . Graduate School at Shenzhen Tsinghua University Shenzhen China 《中国科学G辑(英文版)》2005,48(3):336-344
Fluorescence fluctuation spectroscopy (FFS)[1,2] is a kind of fluorescence detection techniques suitable for monitoring interaction and transportation processes in a small detection zone of biological systems. FFS detects and analyzes the fluorescence signal of the fluorescent molecules in the detection zone to retrieve the dynamical parameters of the system including concentration, diffusion coefficient and interaction constants. FFS utilizes various statistical methods to analyze the data,… 相似文献
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Luchowski R Sabnis S Szabelski M Sarkar P Raut S Gryczynski Z Borejdo J Bojarski P Gryczynski I 《Journal of luminescence》2010,130(12):2446-2451
Concentration is a key determining factor in the fluorescence properties of organic fluorophores. We studied self-quenching of disodium fluorescein (uranin) fluorescence in polyvinyl alcohol (PVA) thin films. The concentration dependent changes in brightness and anisotropy were followed by a lifetime decrease. We found that at a concentration of 0.54 M, the lifetime decreases to 7 ps. At a concentration of 0.18 M the lifetime was 10 ps with the relatively high quantum yield of 0.002. In these conditions the fluorescence intensity decay was homogeneous (well approximated by a single lifetime). We realized that such a sample was an ideal fluorescence lifetime standard for spectroscopy and microscopy, and therefore characterized instrument response functions for a time-domain technique. We show that self-quenched uranin enables measurements free of the color effect, making it a superior choice for a lifetime reference over scattered light. 相似文献