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1.
Meso-5,10,15,20-四(4-甲氧基苯基)卟啉荧光熄灭法测定铅   总被引:3,自引:0,他引:3  
基于铅对荧光试剂 Meso- 5 ,10 ,15 ,2 0 -四 (4 -甲氧基苯基 )卟啉 (TMOPP)的荧光熄灭 ,建立了测定微量铅的荧光分析方法。在 p H9.5的 Tris- HCl缓冲体系中 ,最大激发波长和发射波长分别为 4 2 4 nm和6 5 7nm,测定铅浓度的线性范围为 5 .5 0× 10 -7— 7.5 0× 10 -5mol/ L,检出限为 8.5 0× 10 -8mol/ L。用于环境水样中铅含量的分析 ,结果满意。  相似文献   

2.
钙黄绿素-Pd2+荧光光度法测定甲基对硫磷   总被引:2,自引:1,他引:1  
报道了甲基对硫磷的荧光光度测定方法.在pH=7.4的缓冲溶液中钙黄绿素有荧光,Pd2+与其反应使其荧光消失.由于甲基对硫磷与Pd2+形成的配合物比钙黄绿素-钯配合物更稳定,加入后可使钙黄绿素游离出来而重显荧光,激发波长和发射波长分别位于492nm和512nm,测得游离的钙黄绿素荧光强度与甲基对硫磷浓度在1.0×10-7-1.2×10-6mol/L范围内呈良好的线性关系,检出限为5×10-8mol/L,空白样品加标回收率为87%-108%,相对标准偏差小于4%.  相似文献   

3.
邻苯二甲醛的荧光光度法测定   总被引:4,自引:0,他引:4  
郑洪  朱广华 《光谱实验室》2003,20(4):533-536
在 p H11.0的缓冲介质中 ,邻苯二甲醛与 l-组氨酸形成的复合物在 5 4 0 nm处发射出强烈荧光 ,由此建立了邻苯二甲醛的荧光光度分析新方法 ,并系统的研究了邻苯二甲醛与 l-组氨酸结合的反应条件。本法检出限为 1.2× 10 -8mol/ L,荧光强度与浓度在 4 .0× 10 -8— 4 .0× 10 -6 mol/ L范围内呈良好的线性关系。将该法用于消毒剂中邻苯二甲醛的测定 ,回收率为 95 .8%— 10 6.5 %。  相似文献   

4.
基于铁(Ⅲ)对荧光试剂邻苯二酚的荧光熄灭,建立了测定微量铁的荧光分析方法.在0.3mol/L的HCl介质中,最大激发与发射波长分别为277nm和313nm,铁的荧光强度与浓度呈良好的线性关系,测定铁浓度的线性范围为1.59×10-5-1.26×10-3mol/L,检出限为7.14×10-6mol/L,常见的共存离子不干扰测定.本方法用于环境水中铁(Ⅲ)的测定,结果满意.  相似文献   

5.
测定了胰腺肿瘤细胞膜上的潘生丁在乙腈 -水溶液中的荧光光谱和强度 ,并推算出胰腺肿瘤细胞膜上核苷载体的数量。潘生丁的浓度与其荧光强度在 1.0× 10 -12 — 5 .0× 10 -5mol/ L范围内呈线性关系 ,检出限为 2 .8× 10 -13 mol/ L(1.4× 10 -4ng/ m L) ,相对标准偏差为 2 .7%。  相似文献   

6.
微量元素对B族维生素同步荧光分析法影响的研究   总被引:4,自引:1,他引:3  
本文探讨了钙、镁、铜等 7种微量元素对维生素 B1、B2 、B6的同步荧光光谱的影响。实验结果表明 ,当Ca2 + 的浓度为 1.0× 10 -3mol/ L时 ,VB2 、VB6的同步荧光光谱有显著的增强作用 ;而其他微量元素对VB1、VB2 、VB6的同步荧光有不同程度的猝灭或增强作用。利用 Ca2 + 对 3种维生素荧光强度的增敏作用 ,可以有效地提高同步荧光分析法同时测定 VB1、VB2 、VB6的灵敏度 ,VB1、VB2 、VB6的校准曲线的线性范围分别为 2 .4× 10 -7— 9.0× 10 -6、1.1× 10 -7— 4 .6× 10 -6、7.1× 10 -7— 2 .0× 10 -6mol/ L。采用本方法测定混合样品 ,样品不需分离 ,操作简便、快速  相似文献   

7.
李远刚  李华静 《光谱实验室》2010,27(4):1339-1342
在HAc-NaAc缓冲介质中,铝(Ⅲ)对环丙沙星有荧光增强作用,据此建立了测定微量铝的荧光分析法。选择最大激发与发射波长分别为273nm和439nm,在pH=4.00的HAc-NaAc缓冲溶液介质中,环丙沙星的荧光强度变化与铝(Ⅲ)浓度呈良好的线性关系,线性范围为1.05×10-4—7.35×10-2mol/L,方法的检出限为5.6×10-6mol/L,校准曲线回归方程式为F/F0=0.272lgC+2.764。本方法用于实际样品中铝(Ⅲ)的测定,结果满意。  相似文献   

8.
李莹  杜虹  赵常志 《光谱实验室》2010,27(4):1498-1502
通过电化学氧化肾上腺素,使其生成羟基吲哚类荧光物质(λem=490nm),实现了药物中肾上腺素含量的电化学氧化-荧光检测。研究结果表明,荧光强度与肾上腺素浓度分别在2.00×10-7—1.00×10-6mol/L和1.00×10-6—4.00×10-5mol/L范围内呈良好的线性关系,检出限为6.70×10-8mol/L。该方法用于药物制剂中肾上腺素含量的测定,相对标准偏差小于1.88%(n=7),加标回收率为88.4%—116.6%。  相似文献   

9.
乔善宝 《光谱实验室》2005,22(4):737-739
盐酸异丙肾上腺素在伯瑞坦-罗比森缓冲溶液(pH=6.80)介质中有很强的荧光。其最大激发波长和发射波长为287nm和622nm。荧光强度与纯盐酸异丙肾上腺素浓度在0.1—2.3mg/L范围内呈线性关系。回归方程F=351.91C(mg/L) 139.02,r=0.9995,检出限为0.0087mg/L。测定注射用针剂中盐酸异丙肾上腺素的含量,方法简单,快捷,灵敏度高,结果令人满意。  相似文献   

10.
表面活性剂敏化稀土荧光探针对环丙沙星药物的测定研究   总被引:1,自引:1,他引:0  
稀土铽离子能与环丙沙星形成络合物,并发射铽离子的特征荧光,加入表面活性剂SDBS能大大增 强体系的荧光强度,由此建立了表面活性剂敏化的铽离子荧光探针测定环丙沙星的方法。用1 cm石英比色 池在激发波长为300 nm,发射波长为545 nm处测定其荧光强度。在最佳测试条件为pH=8.0-8.5,Tb3+ 浓度为5.0×10-5 mol·L-1,SDBS浓度为8.0×10-4 mol·L-1时,环丙沙星的浓度与体系的荧光强度呈线性 关系,线性范围为2.0×10-8nol·L-1-2.5×10-6mol·L-1,相关系数为0.999 6,检出限为4.0×10-9mol· L-1。该法可用于不同剂型环丙沙星药物的测定。  相似文献   

11.
Two-dimensional scanning of a 0.6328 mum guided-light beam has been realized using noncolinear acousto-optic (AO) coplanar Bragg diffraction together with colinear AO guided-mode to substrate radiation-mode conversion in a Z-cut Xpropagation LiNbO3 proton-exchanged (PE) waveguide. The two surface acoustic (SAW) waves utilized are at the center frequencies of 500 and 200 MHz, propagating in the Y and X axes, respectively. Two-dimensional scanning of approximately 720 resolvable light beam spots, namely, 18 40 (horizontal vertical) scanning, has been demonstrated using a light beam of 1.0 mm aperture. The total number of resolvable beam spots can be greatly increased from 720 by simply utilizing SAW transducers of larger bandwidth and a light beam of greater aperture. It should also be possible to significantly increase the diffraction efficiency from 3 % by optimizing the parameters of the PE waveguide and the SAWs.  相似文献   

12.
A simple technique of pulse compression, based on the linear chirp compensation of self-phase modulation (SPM) spectra in dispersion shifted fibers, is demonstrated. The optimization procedure is carried out, for a short span of a single-mode fiber, using a parabolic law, which describes the behavior of the squared output pulse width versus the pump peak power in the case of Gaussian pulses. The experimental results give a minimum pulse duration of 233 fs, which is in good agreement with the model. Shorter and coherent pulses, down to 90 fs, have been obtained by inserting an interference filter at the optical output.  相似文献   

13.
Results of a study of the effect of γ-radiation on the spectral luminescence properties of cadmium tungstate crystals doped with silver, bismuth, and molybdenum cations are presented. Spectral characteristics of the nondnnnoped crystals are briefly described. Absorption and photo and X-ray luminescence spectra of the crystals taken before and after exposure to γ-radiation (5.5·104 Gy) are compared. It is found that the spectral characteristics of the crystals doped with silver, bismuth, and molybdenum cations do not change markedly after the exposure. The relation between the type of impurity-induced defects, individual characteristics of the impurity cations, and the character of the effect of γ-radiation on the spectral luminescence properties of impure crystals is analyzed (preliminarily). Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 64, No. 1, pp. 55–60, January–February, 1997.  相似文献   

14.
The Shanghai Synchrotron Radiation Facility(SSRF)booster ring,a full energy injector for the storage ring,is deigned to accelerate the electron beam energy from 150MeV to 3.5GeV that demands high extraction efficiency at the extraction energy with low beam loss rate when electrons are ramping.Closed orbit distortion(COD)caused by bending magnet field uniformity errors which affects the machine performance harmfully could be effectively reduced by bending magnet location sorting.Considering the affections of random errors in measurement,both ideal sorting and realistic sorting are studied based on measured bending magnet field uniformity errors and one reasonable combination of bending magnets which can reduce the horizontal COD by a factor of 5is given as the final installation sequence of the booster bending magnets in this paper.  相似文献   

15.
A review of schemes for multiple access in fiber optic networks shows that a hybrid of wavelength and code division multiple access (WCDMA) combines the best features of both. In particular, the hybrid scheme retains the large information carrying capacity of wavelength division multiple access (WDMA) and flexibility of code division multiple access (CDMA). In this paper WDMA, optical CDMA (OCDMA), and WCDMA networks are discussed. In OCDMA networks, concept of incoherent and coherent coding including inverse decoding and matched filter is introduced. The delay performance of both WDMA and WCDMA networks, under the simple suboptimum access protocols based on cyclic search, is computed. It has been shown quantitatively that tuning delay significantly affects the delay performance of both WDMA and WCDMA networks. Futhermore, delay performance of WCDMA networks is always better than the WDMA networks for the same tuning delay, load, and number of users.  相似文献   

16.
17.
A transient suppression method for piezoelectric ceramic transducer is presented. By adding some special pedestal pulses to the original transmitted signal, the transient effects in the output of the transducer can be just precisely transmission by use of a narrowband transducer. canceled, thereby achieving the broadband The experimental results show that the transient responses of the sinusoid pulses and coded signals are suppressed successfully and the acoustic waveforms radiated into the surrounding water are improved significantly.  相似文献   

18.
We experimentally demonstrate amplitude squeezed soliton utilizing intensity-dependent self-phase modulation in an asymmetric Sagnac interferometer.The system.whose components are connected via ferrule connector/physical connection(FC/PC)fiber connectors,constitutes all-fiber configuration to generate squeezed soliton.Soliton amplitude reduction measured by homodyne detection is near 4.0 dB below the shot-noise level.Optimal squeezing fields in both simple and compact all-fiber configuration are obtained.  相似文献   

19.
The(2 1)-dimensional nonlinear Schr(o)dinger(NLS)equation with spatially inhomogeneous nonlinearities is investigated,which describes propagation of light in(2 1)-dimensional nonlinear optical media with inhomogeneous nonlinearities.New types of optical modes and nonlinear effects in optical media are presented numerically.The results reveal that the regular split of beam can be obtained in (2 1)-dimensional nonlinear optical media with inhomogeneous nonlinearities,by adjusting the guiding parameter.Furthermore,the stability of beam regular split is discussed numerically,and the results reveal that the beam regular split is stable to the finite initial perturbations.  相似文献   

20.
In the real-world application of face recognition system, owing to the difficulties of collecting samples or storage space of systems, only one sample image per person is stored in the system, which is so-called one sample per person problem. Moreover, pose and illumination have impact on recognition performance. We propose a novel pose and illumination robust algorithm for face recognition with a single training image per person to solve the above limitations. Experimental results show that the proposed algorithm is an efficient and practical approach for face recognition.  相似文献   

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