数字显微全息中二次项相位误差的补偿

针对预放大式数字离轴显微全息技术中二次项相位误差,提出一种基于单幅全息再现的补偿方法.该方法是在显微物镜后焦平面处实现该相位误差的数学补偿,相位误差补偿过程具有调整参量单一化的优点.同时,利用显微物镜的后焦平面上物点特征实现了最佳再现距离的自动判断.该文以相位光栅为实验样本,对该方法进行了实验例证,实验结果验证了本文所提出二次项相位误差的补位误差的补偿方法的正确性和有效性.     

Correction of aberration for a high-resolution electron hologram by means of the amplitude contrast criterion of image wave

In order to further improve the resolution for a high-resolution electron hologram, the aberration working on the hologram must be corrected. Since it is rather difficult to precisely control aberration coefficients in the experimental stage, we proposed an amplitude contrast D criterion of imaging wave to determine the working aberration from the hologram itself. In the determination or correction of the aberration, we assume a symmetrical aberration function is parameterized only by a spherical aberration coefficient and a defocus value. First, D is calculated from a holographically reconstructed imaging wave of YBa(2)Cu(3)O(7-x) for each combination of these parameters. The working aberration on the imaging wave is determined from the combination of the parameters by noting the maximum or minimum D of the imaging wave at some specifically chosen thickness regions. The theoretical validity for the D criterion is then proved with three-beam dynamical diffraction formula. Finally, the 'experimental' examination for the D criterion is successfully performed on the reconstructed image wave for the Sigma=9 interface structure of a wedge-shaped silicon sample.     

无透镜傅里叶变换数字全息术中非共面误差的自动补偿算法

无透镜傅里叶变换数字全息术对活细胞进行显微相衬成像测量时,由于细胞处于运动状态导致记录物体与参考点源难以共面,从而引入成像误差.本文提出了一种有效可靠的基于位相分布的自动对焦再现算法,只需通过一幅全息图,即可确定出记录距离的优化数值解,并结合一次位相线性拟合,成功补偿掉非共面误差.将这种算法应用到活体宫颈癌细胞的相衬成像实验中获得了较为理想的实验结果,表明了这种自动补偿非共面误差算法的可行性. 关键词： 数字全息 无透镜傅里叶变换全息 自动对焦 位相畸变校正     

像面数字全息的重建相位误差分析和改善

像面数字全息是数字全息技术中常用的测量和成像方式,它通常采用离散傅里叶变换和频率滤波的方法进行物光波的重建.本文讨论了这些算法对重建相位的影响.首先分析了频谱泄露对于相位误差的影响,结果表明当采样周期为整数时,重建相位误差很小,因此具有极高的相位重建精度;而当不满足整周期采样时,相位重建误差有了明显的增加.为了改善频谱泄露所引起的相位误差,采用Hanning函数对数字全息图进行了预处理,结果表明Hanning窗的加入能够有效地提高重建相位的准确程度.     

Self-aligned imaging system using phase conjugate readout from elementary holographic grating

We propose a self-aligned imaging system (SAIS) using phase conjugate readout from an elementary holographic grating. The SAIS provides a huge amount of interconnections between a lot of points on two corresponding imaging planes without troublesome mechanical alignment. In the SAIS, a hologram for one-to-one interconnection is used for many-to-many interconnections. In addition, the SAIS has the capability of correcting aberration. Preliminary experiments verify the principle and the capability of the SAIS.     

Wave-front curvature compensation of polarization phase-shifting digital holography

An on-axis polarization phase-shifting digital holographic microscope, based on a normal upright optical microscope, is constructed to quantitatively measure both the amplitude and phase distributions of specimen. The condenser lens and microscope objective employed in the object beam path enhance the illumination and magnification of the image, however, they induce additional phase aberration of the object wave. The physical formation of the phase aberration is theoretically analyzed, and a formula for the object wave front involving the phase aberration in the CCD plane is derived. The phase aberration can be eliminated in the reconstruction procedure by measuring a specimen-free hologram and then fitting the aberration phase with a least square ellipsoidal model to determine the parameters of the system. This phase aberration compensation procedure also reduces some of noises in the reconstructed phase of the specimen. The practicability of this method is demonstrated by a test experiment on microlenses.     

通过保留相位进行全息图信息压缩的方法

从全息图的特点出发,研究了全息图的记录和再现原理,提出了一种对离轴菲涅耳全息图信息压缩的方法。对全息图进行傅里叶变换,滤除零级噪声及物光波的共轭波前,仅仅保留物光波的相位信息,而后以采用较低的采样频率对全息图重新抽样,从而大大减少了数字全息图的信息冗余。压缩后的全息图也可以获得良好像质的再现像。理论和实验均证明了该方法的可行性,为全息图的信息存贮与传输提供一条新的途径。     

干涉成像系统传递函数的数值分析

干涉仪系统传递函数能有效地表征系统相位成像的性能。通过假设干涉成像系统是复振幅的线性平移不变系统,模拟计算正弦相位光栅和相位台阶这两类标准相位物体的成像,确定干涉仪系统传递函数。数值分析结果表明:系统传递函数随着波像差的增加而减小;干涉成像系统对小幅度相位（远小于1 rad）成像是近似线性的,而对大幅度相位(大于0.5 rad）成像则是明显非线性的。当正弦相位的幅度为1时,系统传递函数在1/2和1/3截止频率处出现明显的急剧下降。高度为/2的相位台阶成像时,系统传递函数随着空间频率的增加而缓慢地降低。 The performance of phase imaging in interferometric imaging system is well characterized by the system transfer function (STF). The STF of the interferometric imaging system is analyzed numerically by assuming that the system is linear and shift-invariant for the complex field. Two standard phase objects, sinusoidal phase grating and phase step, are employed and simulated to determine the STF. Numerical simulation results show that the STF decreases as the wavefront aberration of interferometric imaging system increases. It also shows that the interferometric imaging system is approximately linear for small phase (far less than 1 rad) but explicitly nonlinear for large phase (larger than 0.5 rad). The STF has a visible drop at one half or one third of the cut-off frequency of the imaging system when the amplitude of sinusoidal phase is 1 rad. For a phase step with a height of /2 rad, the STF has no visible drop but decreases slowly with the increasing of spatial frequency. The results provide a useful guidance to the design of interferometer and the measurement of STF and power spectrum density in experiment.     

一种有效解决离轴数字全息相图倾斜畸变的数字参考平面方法

离轴数字全息中使用倾斜的平面参考波以消除成像中的零级衍射和共轭像是一种简捷和常用的方法,然而该方法遇到的一个困扰是,由于倾斜参考波引入了附加的载波频率并很难通过实验测量准确地获得附加的载波频率值或倾角,因此会导致重建的相图出现一定的倾斜畸变而无法完全修正.本文提出了一种数字参考平面算法以解决这一问题.该算法利用重建相图的平坦区域选点构建一个能准确表征相图倾斜的数字参考平面,并建立该平面参量与参考波载波频率的数学关系和作为随后相图畸变修正迭代计算的判据.该算法简单有效,不仅能实现对倾斜相位畸变的准确修正,而且能准确地获得倾斜平面参考波的附加载波频率.由于在相位解包裹重建中结合了抑噪处理,因此该方法在环境和系统噪声的影响下仍然有效,实验结果验证了理论设计的有效性.     

The effect of tilt aberration and astigmatism of turbulent atmosphere on the intensity distribution of a vortex carrying Gaussian beam

The effect of tilt and astigmatism aberration of the turbulent atmosphere on the intensity distribution of a focused vortex carrying Gaussian beam was investigated based on the extended Fresnel-Kirchhoff diffraction integral and the quadratic approximation of phase structure function. Our results have shown that the intensity distribution on the focal plane in the effect of tilt aberration changes with the turbulent strength, the propagation distance and the topological charge of the initial beam. The propagation distance is larger, the focal spot size will be larger, and the central dip will be less deep. It is also noticed that the intensity distribution of a beam with single topological charge is affected more by tilt aberration in comparison to the beam with double topological charge. The effect of astigmatism on the intensity distribution is quite less than that of tilt aberration. For focused beam propagation in atmosphere, the effect of the tilt aberration on the intensity distribution of optical vortex beam is the main effect of total turbulent aberration.     

Synthetic aperture fourier holographic optical microscopy

We report a new synthetic aperture optical microscopy in which high-resolution, wide-field amplitude and phase images are synthesized from a set of Fourier holograms. Each hologram records a region of the complex two-dimensional spatial frequency spectrum of an object, determined by the illumination field's spatial and spectral properties and the collection angle and solid angle. We demonstrate synthetic microscopic imaging in which spatial frequencies that are well outside the modulation transfer function of the collection optical system are recorded while maintaining the long working distance and wide field of view.     

结合线性回归的离轴数字全息去载波相位恢复算法

为实现仅用一幅离轴数字全息图便能直接恢复相位,提出一种利用空间载波相移技术(spatial carrier phase shift, SCPS)和线性回归相结合的离轴数字全息去载波相位恢复算法.首先,利用SCPS将一幅离轴数字全息图分为四幅含有载波相移的全息图,其中载波相移由沿行、列两个方向的正交载波所引入;然后,将四幅载波相移全息图作为输入,将所求物体相位和两个正交的载波作为未知量,结合最小二乘法和线性回归同时求出载波和相位信息.相较于已有的去载波技术,本算法无需背景全息图作为参考,便可准确地去除载波,实现高质量的相位重建.本文结合数值仿真和具体实验结果验证本算法的有效性和优越性.     

Wave-front reversal of multifrequency continuous emission by Bragg scattering from phase regular inhomogeneities

The possibility of wave-front reversal of continuous multifrequency emission with a wide line spectrum (in particular, emission of lasers operating on cascade vibrational-rotational transitions of diatomic molecules, e.g., HF or CO) is analyzed; the reversal is based on Bragg scattering from a spatial phase hologram that is recorded in a resonance-absorbing medium by emission with a frequency different from any of the reversed-signal frequencies. The mechanism of formation of a spatial phase hologram and the features of optical Bragg scattering on such structures are analyzed. It is shown that a scattered wave-front is complex conjugate to one of the waves recording the phase grating. An optical system for wave-front reversal is proposed and the Bragg scattering efficiency is estimated for a particular example. The analysis indicates that the proposed system does not exhibit frequency selectivity and is basically suitable for wave-front reversal of emission with a broad line spectrum. The main problem is to choose a medium with resonance absorption at the center of a multifrequency emission spectrum and with complete transparency for the reversed signal.     

Phase aberration compensation of digital holographic microscopy based on least squares surface fitting

Digital holographic microscopy allows the numerical reconstruction of the complex wavefront of samples, especially biological samples such as living cells. In digital holographic microscopy, a microscope objective is introduced to improve the transverse resolution of the sample; however a phase aberration in the object wavefront is also brought along, which will affect the phase distribution of the reconstructed image. We propose here a numerical method to compensate for the phase aberration of thin transparent objects with a single hologram. The least squares surface fitting with points number less than the matrix of the original hologram is performed on the unwrapped phase distribution to remove the unwanted wavefront curvature. The proposed method is demonstrated with the samples of the cicada wings and epidermal cells of garlic, and the experimental results are consistent with that of the double exposure method.     

纯位相信息自聚焦计算全息匹配滤波器

本文介绍一种用计算全息方法制作的纯位相信息自聚焦匹配滤波器。它在编码时取滤波函数的振幅为１,仅对位相进行编码;并通过对傅里叶谱加入二次位相因子代替傅里叶透镜,使其具有自聚焦功能。与常规的方法（光学全息法或计算全息法）所获得的匹配滤波器相比较,具有原理上更合理,滤波器的制作和匹配光路更简单,也更容易实现匹配等特点。所得到的匹配实验结果也很清晰,这与理论是完全符合的。     

A novel method of high resolution imaging through random perturbation wave front

A novel method is proposed for eliminating the effect of the traditional optical aberration on high resolution imaging by random perturbation wave front and digital image processing. A random phase mask, whose phase spectrum fluctuation is accordant with Kolmogorov distribution, is positioned near the aperture stop of optical system, making the optical aberration image become random perturbation image, that is, the intermediate image. The blind deconvolution algorithm based on maximum-likelihood estimation technique is used to restore the intermediate image acquired by a digital detector. The effects of optical aberrations and the noise on the restoration image are explored. To demonstrate the validity of the method proposed, the computer simulation and laboratory experiments are carried out for the imaging of the optical system with primary aberration. The results have shown that the present method is well suited for effectively improving the imaging quality of the optical system with certain aberration, thus making the optical system resolution close to or reach the diffraction-limit of the optical system.     

Multiple information encryption by user-image-based gyrator transform hologram

A novel multiple information encryption by user-image-based gyrator transform hologram is proposed. In encryption process, each channel of the user image is phase encoded, modulated by random phase function and then gyrator transformed to get the gyrator spectrum of user image. Subsequently, each channel of the secret image is normalized, phase encoded, multiplied by modulated user image, and then gyrator transformed to obtain the gyrator spectrum of secret image. The encrypted digital hologram is recorded by the interference between the gyrator spectrum of user image and the spherical wave function. Similarly, the digital hologram for decryption is recorded by the interference between the gyrator spectrum of secret image and the spherical wave function. The multiple encrypted digital holograms are multiplexed into a final encoded hologram and the corresponding digital holograms for decryption are multiplexed into a final hologram for decryption. The wavelength and radius of the spherical wave function, and angle of gyrator transform are all essential keys for decryption. The proposed system has two main features. First, the encrypted hologram has no information about secret image. Second, the hologram for decryption used as identification key. Consequently the two marked security layers of information protection are achieved. The proposal can be realized by optoelectronic system. Numerical simulation results demonstrate the feasibility and security of the proposed technique.     

优化的数字全息显微成像系统

基于预放大数字全息显微系统的全息图记录与再现过程及其点扩散函数的分析, 从成像分辨率、成像质量及实现的难易程度等方面对数字全息显微中六种常见记录光路系统的成像性能进行了对比研究. 结果表明, 像面数字全息术具有最高的成像分辨率及成像质量, 其成像分辨率与记录器件的光敏面尺寸无关, 该系统对信息的记录是完整的, 而且记录过程不必考虑物体被照亮区域的大小, 再现过程非常简单, 是优化的数字全息显微成像系统. 等波面弯曲的物参光像面数字全息术非常有利于位相解包裹及位相畸变补偿的正确进行, 该系统更适合于位相显微. 实验结果验证了理论分析的正确性. 关键词： 显微数字全息 预放大数字全息 像面数字全息 分辨率     

新型轻量化可见光光谱仪成像系统研究与分析

利用衍射光学元件消色差和对波面进行任意整形及自由曲面光学元件校正像差的特性,将衍射光学元件和自由曲面光学元件应用于传统可见光光谱成像光谱仪成像系统中,提出了一种新型的可见光光谱成像光谱仪成像系统,基于衍射光学元件独特的负色散特性,用一片折射/衍射混合光学元件代替传统的双胶合透镜来实现消色差,基于自由曲面光学元件的多自由度特性,在系统中引入两个自由曲面光学元件对系统中的像差进行有针对性的校正,针对具体的设计实例给出了详细的设计步骤,设计结果表明,在相同的参数指标前提下,新型系统仅为4片式结构,和传统系统相比,重量减轻22.9%,总长缩短26.6%,最大口径减小30.6%,调制传递函数值最大提升为1.0视场,调制传递函数值提高0. 35,最大畸变减小1.6%,最大垂轴像差减小56.4%,轴向色差减小59.3%,新型系统各方面的性能都得到了较大的改进,为现代高性能、轻小型可见光光谱成像光谱仪成像系统设计提供了一种新的途径。     

复数滤波器在数字全息图处理中的应用

数字全息图在微电路检测、粒度分析、透明场测量等小孔径、小视场对象测量和细胞观测等显微测量方面有着广泛的应用前景。根据离轴数字全息图的频谱特点,提出采用复数滤波器,利用计算机及相关算法对离轴数字全息图进行滤波,消除其零级及共轭像,同时可以得到全息图的相位分布。讨论了复数滤波器的设计方法,给出了实验结果。为数字全息图的压缩、再现和相息图制作提供了方便。