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1.
李小芳  冯小强  朱元成 《发光学报》2017,38(10):1359-1365
以糠醛和壳寡糖反应生成的席夫碱,与不同摩尔比的Cu2+配位合成配合物FCOS-Cu11、FCOS-Cu13和FCOS-Cu31,并研究配合物与DNA之间的作用机制。采用循环伏安、紫外-可见吸收光谱、粘度和DNA熔点测定的方法,分别研究了席夫碱及配合物与DNA之间的作用机制。合成的3种配合物具有电化学活性。加入DNA后,配合物的氧化峰电流减小,峰电位微弱移动。配合物的吸收峰强度减色显著,同时伴有峰位红移现象。配合物加入后,DNA的相对粘度和熔点呈增大趋势。结果表明,席夫碱及配合物与DNA之间存在嵌插结合,且3种配合物中的Cu2+含量与DNA的嵌插作用强度有关,结合强度依次为FCOS-Cu11FCOS-Cu13FCOS-Cu31。  相似文献   

2.
合成了稀土镧 (La)与 2 ,2 联吡啶 (dpy) ,1,10 邻菲咯啉 ( phen)的混配物 ,并进行了红外和热分析的表征 ,证实了其混配物的结构为La(dpy) ( phen)Cl3 。并对其与DNA作用的紫外 可见光谱和共振光散射光谱作了初步的研究。研究结果表明 ,混配物的吸收峰随着DNA的加入而发生减色效应 ,而DNA浓度继续增高 ,吸收峰又增高 ,说明此混配物与DNA先是嵌入结合 ,而后伴随有静电结合。而此混配物在 4 70nm处的共振光散射峰随着DNA的加入增强 ,则表明混配物在DNA分子表面进行长距离自组装。研究证明所合成的配合物能够与DNA作用 ,可作为DNA的荧光探针  相似文献   

3.
合成了一种新型的三元铜配合物[Cu(phen)(SA)_2]·H_2O(phen=1,10-邻菲啰啉,SA=水杨酸),并以鲱鱼精DNA为靶点,通过紫外吸收光谱法、循环伏安法、差分脉冲伏安法和DNA粘度滴定实验,探讨了配合物与DNA的键合方式。结果表明,SA的羧酸根离子与Cu~(2+)单齿配位,phen的两个氮原子与Cu~(2+)呈双齿配位,形成的配合物在0.489 V/0.050 V处呈现一对明显的准可逆氧化还原峰,并且中心Cu~(2+)在玻碳电极上的反应主要由扩散过程控制。配合物与DNA作用时,可观察到配合物的紫外光谱出现明显的减色效应,但是红移现象不明显,氧化还原峰的电流减小,峰电位发生正移,并且DNA的粘度随配合物的加入而增大。结论认为,配合物以嵌插方式与DNA发生作用形成1∶1的复合物,但插入程度较弱(结合常数为1.55×10~4L·mol~(-1))。  相似文献   

4.
冯小强  李小芳  杨声 《光谱实验室》2013,30(4):1960-1963
采用循环伏安法和紫外吸收光谱研究了壳聚糖铜配合物与鲱鱼精DNA的相互作用。结果发现:随壳聚糖铜浓度的增加,DNA的紫外吸收光谱呈增色效应和较小的红移;壳聚糖铜离子在电极上的反应主要由扩散过程控制,加入DNA后氧化还原峰电流降低,且式量电位正移,说明壳聚糖铜与DNA以嵌入方式生成一种非电活性超分子复合物,导致壳聚糖铜配合物的峰电流降低。实验结果表明壳聚糖铜与DNA的相互作用模式为嵌入和静电混合作用。  相似文献   

5.
紫外-可见光谱法和循环伏安法研究了1-(4-氯苯甲酰基)-4-[1'-N'-(2',3',4',6'-四-o-乙酰基)-D-吡喃葡萄糖基]氨基硫脲与DNA的相互作用.该试剂与DNA作用后,引起DNA的紫外特征吸收峰呈明显的减色效应和红移现象,通过该试剂对溴化乙锭-DNA紫外-可见光谱和循环伏安曲线的影响,证明它与DNA发生了嵌插作用.  相似文献   

6.
紫外 -可见光谱法和循环伏安法研究了 1- (4 -氯苯甲酰基 ) - 4- [1′- N′- (2′,3′,4′,6′-四 - o-乙酰基 ) - D-吡喃葡萄糖基 ]氨基硫脲与 DNA的相互作用。该试剂与 DNA作用后 ,引起 DNA的紫外特征吸收峰呈明显的减色效应和红移现象 ,通过该试剂对溴化乙锭 - DNA紫外 -可见光谱和循环伏安曲线的影响 ,证明它与DNA发生了嵌插作用。  相似文献   

7.
以糠醛和对氨基苯磺酸反应合成席夫碱配体,与稀土离子配位制得相应的稀土配合物,并运用光谱法研究配合物与DNA之间的作用机制,为新型抗癌药物的设计开发提供依据。采用元素分析、红外和紫外光谱对配合物性质进行表征测试,确定其化学组成为REL(OH),RE为La~(3+)、Nd~(3+)、Eu~(3+),L为去质子形式的希夫碱配体。此外,运用光谱法和循环伏安法研究了REL(OH)配合物与鲱鱼精DNA之间的作用方式。配合物在加入DNA后,特征吸收峰强度发生明显的减色效应,但峰位红移不明显。配合物能够猝灭中性红-DNA体系的荧光。Fe(CN)_6~(3-)/~(4-)的氧化还原峰电流在配合物加入后减小,式量电位有正移趋势,表明配合物、Fe(CN)_6~(3-)/~(4-)与DNA的作用存在竞争。研究结果表明:这3种席夫碱稀土配合物与鲱鱼精DNA的作用均属于嵌插,且含有不同稀土离子的配合物与DNA的作用强弱不同,作用大小次序为Eu配合物La配合物Nd配合物席夫碱,这可能与DNA和配合物中的稀土离子所发生的作用有关。  相似文献   

8.
染料木素铬(Ⅲ)配合物与DNA相互作用的研究   总被引:5,自引:0,他引:5  
在pH7.2 Tris缓冲溶液中,采用紫外、DNA熔点、荧光、粘度等手段研究了染料木素铬(Ⅲ)配合物与小牛胸腺DNA(ctDNA)的作用机制,探讨了其作用模式。当加入一定量的ctDNA时,染料木素铬(Ⅲ)配合物的紫外吸收光谱的最大吸收峰产生明显的减色效应;而配合物体系的荧光强度、共振光散射信号随ctDNA的加入逐渐增强。配合物的存在能有效猝灭EB-DNA体系的荧光,且猝灭方式为静态猝灭。同时ctDNA的熔点和ctDNA溶液的粘度随配合物的加入而增大。据此推断,染料木素铬(Ⅲ)配合物与ctDNA之间具有较强的作用,配合物主要以插入方式与DNA结合,二者的键合常数为1.9×105L·mol-1。该研究结果提示染料木素铬(Ⅲ)配合物有望作为抗癌活性候选药物,值得进一步深入研究。  相似文献   

9.
采用紫外吸收光谱、荧光光谱和循环伏安法,研究了水杨醛缩壳寡糖-Cu配合物与鲱鱼精DNA的相互作用.发现DNA使得水杨醛缩壳寡糖-Cu的紫外吸收光谱呈现增色效应;水杨醛缩壳寡糖-Cu离子在电极上的反应主要由扩散过程控制,加入DNA后氧化还原峰电流急剧降低.结果表明水杨醛缩壳寡糖-Cu与DNA分子之间以静电模式而发生了相互作用.  相似文献   

10.
李晨  李剑峰  段武彪  刘博 《发光学报》2016,37(2):158-164
合成了新型尾式5-(2-(α-咪唑基间甲基苯甲酰胺基)苯基)-10,15,20-三苯基卟啉(H2TPP-Bz Im)及其Fe、Co、Zn配合物,并用核磁、质谱、红外光谱、紫外-可见光谱等对其化合物进行了表征,通过紫外吸收、荧光分析、循环伏安法研究了化合物的光电性质。结果表明,与四苯基卟啉(H2TPP)相比,该尾式卟啉及其配合物的紫外和荧光光谱均出现较明显红移。Fe和Co卟啉的循环伏安曲线表明不仅发生了卟啉环上的氧化还原反应,还发生了金属离子的氧化还原反应。  相似文献   

11.
Novel different substitued polypyridine ligands 4-((4-(1H-imidazo[4,5-f][1,10]phenanthroline-2-yl)phenoxy)methyl)benzaldehyde (BA-PPY), (E)-N-(4-((4-(1H-imidazo[4,5-f][1,10]phenanthroline-2-yl)phenoxy)methyl)benzylidene)-pyrene-4-amine (PR-PPY), (E)-N-(4-((4-(1H-imidazo[4,5-f][1,10] phenanthroline-2-yl)phenoxy)methyl)benzylidene)-1,10-phenanthroline-5amine (FN-PPY), 2-(4-(bromomethyl)phenyl)-1H-imidazo[4,5-f][1,10] phenanthroline (BR-PPY), 2-(4-(azidomethyl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline (N3-PPY) and triazole containing polypyridine ligand 3,4-bis[(4-(metoxy)-1,2,3-triazole)1-methylphenyl)-1H-imidazo[4,5-f][1,10]phenanthroline)] benzaldehyde (BA-DIPPY) and Ruthenium(II) complexes were synthesized and characterized. Their photopysical properties were investigated. The complexes RuP(PR-PPY), RuB(PR-PPY, RuP(FN-PPY) and RuB(FN-PPY) exhibited a broad absorption bands at 485, 475, 476, and 453 nm, respectively, assignable to the spin-allowed MLCT (dπ–π*) transition. The emission maxima of the pyrene-appended polypyridine ligand PR-PPY was observed at λems = 616 nm and the phenanthroline-appended polypyridine ligand FN-PPY was observed at λems = 668 nm. And the emission maxima of the complexes RuP(PR-PPY), RuB(PR-PPY), RuP(FN-PPY) and RuB(FN-PPY) were observed at λems = 646, 646, 685 and 685 nm, respectively. As seen in fluorescence spectra, the fluorescence intensities of the ligands are higher than their metal complexes. This is because of quenching effect of Ruthenium(II) metal on chromophore groups.  相似文献   

12.
Three ruthenium(II) polypyridyl complexes, [Ru(phen)2(mip)](ClO4)2 (1) (phen =1,10-Phenanthroline), [Ru(bpy)2(mip)](ClO4)2 (2) (bpy = 2,2’bipyridyl) and [Ru(dmb)2(mip)](ClO4)2 (3) (dmb = 4, 4′-dimethyl 2, 2′-bipyridine), were synthesized with an intercalative ligand mip (2-morpholino-1H-imidazo[4,5-f][1, 10]phenanthroline) and characterized by 1H, 13C–NMR, IR, UV-vis, mass spectra and elemental analysis. pH effect, ion selectivity (cations, anions) and solvent sensitivity of complexes were studied. The interaction of these complexes with DNA was performed using absorption, emission spectroscopy and viscosity measurements. The experimental results indicated that the two complexes interacted with calf thymus DNA (CT-DNA) by intercalative mode. BSA (Bovine Serum Albumin) protein binding of these complexes was studied by UV-visible and fluorescence techniques. The binding capacity of these complexes was explained theoretically by molecular docking method.  相似文献   

13.
The new ligand 4-(isopropylbenzaldehyde)imidazo[4,5-f ][1,10]phenanthroline (ippip) and its complexes [Ru(phen)2(ippip)]2+(1),[Co(phen)2(ippip)]3+(2),[Ru(bpy)2(ippip)]2+(3),[Co(bpy)2(ippip)]3+(4)(bpy=2,2-bipyridine) and (phen=1,10-phenanthroline) were synthesized and characterized by ES+-MS, 1H and 13C NMR. The DNA binding properties of the four complexes were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that complexes bind to calf thymus DNA (CT-DNA) through intercalation. When irradiated at 365 nm, the complexes promote the photocleavage of pBR322 DNA, and complex 1 cleaves DNA more effectively than 2, 3, 4 complexes under comparable experimental conditions. Furthermore, photocleavage studies reveal that singlet oxygen (1O2) plays a significant role in the photocleavage.  相似文献   

14.
采用稳态发光光谱、瞬态发光动力学测量等手段,对两种钉配合物[Ru(bpy)3](ClO4)2和[Ru(bpy)2 HPIP](ClO4)2的发光性质进行了研究.稳态发光光谱表明[Ru(bpy)2 HPIP](ClO4)2发光明显偏弱;皮秒瞬态发光动力学测量显示[Ru(bpy)2 HPIP](ClO4)2的激发态弛豫过程...  相似文献   

15.
采用密度泛函的方法,结合导体极化连续模型研究了水溶性二价钌-甲基咪唑类配合物[Ru(MeIm)4iip]2+ (1)、[Ru(MeIm)4tip]2+(2)和[Ru(MeIm)42ntz]2+ (3)的电子结构、DNA的键合倾向及构效关系.在水溶液中几何优化的基础上分析了配合物的电子结构特征,并合理解释了配合物与DNA的键合倾向.计算结果表明,在主配体上用噻吩代替咪唑取代基可以有效提高配合物与DNA的键合力;同时,在主配体的骨架上引入强电负性的N原子及NO2基团可以明显降低配合物最低未占据分子轨道能量及前沿分子轨道能量差.基于以上计算结果,预测所设计的配合物3具有最大的DNA键合力常数.另外,详细分析了配合物1、2的构效关系及抗肿瘤作用机理,并预测了配合物3的抗肿瘤活性.最后,用含时密度泛函方法对配合物的电子吸收光谱进行了计算和模拟,并与实验结果进行了对比分析.  相似文献   

16.

A new ligand FIPB?=?5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)furan-2-yl-2-boronic acid, having three cobalt(III) polypyridyl complexes [Co(phen)2(FIPB)]3+(1) {FIPB?=?5-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)furan-2-yl-2-boronic acid}, (phen?=?1,10-Phenanthroline), [Co(bpy)2(FIPB)]3+(2) (bpy?=?2,2’bipyridyl), [Co(dmb)2(FIPB)]3+(3) (dmb?=?4, 4′-dimethyl 2, 2′-bipyridine) have been synthesized and characterized by elemental analysis, ES-MS,1H-NMR, 13C-NMR, UV-Vis and FTIR. Their DNA binding behavior has been explored by various spectroscopic titrations and viscosity measurements, which indicated that all the complexes bind to calf thymus DNA by means of intercalation with different binding strengths. The binding properties of these all three complexes towards calf-thymus DNA (CT-DNA) have been investigated by UV-visible, emission spectroscopy and viscosity measurements.The experimental results suggested that three Co(III) complexes can intercalate into DNA base pairs,but with different binding affinities. Photo induced DNA cleavage studies have been performed and results indicate that three complexes efficiently cleave the pBR322-DNA in different forms. The three synthesized compounds were tested for antimicrobial activity by using Staphylococcus aureus and Bacillus subtilis organisms, these results indicated that complex 1 was more activity compared to other two complexes against both tested microbial strains. The in vitro cytotoxicity of these complexes was evaluatedby MTT assay, and complex 1 shows higher cytotoxicity than complex 2 and 3 on HeLa cells.

  相似文献   

17.
The novel ligand (dmbip) 2-(4-N, N-dimethylbenzenamine)1H-imidazo[4, 5-f][1, 10]phenanthroline and its complexes [Ru(phen)2dmbip]2+ (1), [Ru(bpy)2dmbip]2+ (2), [Co(phen)2dmbip]3+ (3) and [Co(bpy)2dmbip]3+ (4) [where phen?=?1, 10-phenanthroline, bpy?=?2, 2-bipyridine], have been synthesized and characterized by elemental analysis, IR, UV-Vis, 1H NMR, 13C NMR and Mass spectra. The DNA binding properties of the complexes were investigated by absorption, emission, quenching studies, light switch “on and off”, salt dependent, sensor (cation and anion) studies, viscosity measurements, cyclic voltammetry, molecular modeling and docking studies. The four complexes were screened for Photo cleavage of pBR322 DNA, antimicrobial activity and cytotoxicity. The experimental results indicate that the four complexes can intercalate into DNA base pairs. The DNA-binding affinities of these complexes follow the order [Ru(phen)2dmbip]2+ > [Co(phen)2dmbip]3+ > [Ru(bpy)2dmbip]2+ > [Co(bpy)2dmbip]3+.  相似文献   

18.
赵宝锋  唐怀军  余磊  王保争  文尚胜 《物理学报》2011,60(8):88502-088502
研究了掺杂离子型铱配合物的单发光层聚合物白光器件.根据二元互补色获得白光的原理,所采用离子型橙光材料为六氟磷酸合[二(2-(萘基-1-基)吡啶)(1-乙基-2-(9-(2-乙基己基)-9H-咔唑-3-基)-1H-咪唑并[4,5-f][1,10]菲啰啉)铱(Ⅲ)]([(npy)2Ir(c-phen)]PF6),天蓝光材料为二(2-(4,6-二氟苯基)吡啶-N,C(2))吡啶甲酰合铱(Firpic).器件结构为氧化铟锡/苯磺酸掺杂聚乙烯基二氧噻吩(40 nm)/发光 关键词: 聚合物发光二极管 白光 二元互补色 离子型铱配合物  相似文献   

19.
Three Ruthenium(II) polypyridine complexes, [Ru(phen)2(mipc)]2+(1), [Ru(bpy)2(mipc)]2+ (2) and [Ru(dmb)2(mipc)]2+(3) [mipc?=?2-(6-methyl-3-(1H-imidazo[4, 5-f][1,10]-phenanthroline-2-yl)-4H-chromene-4-one, phen?=?1,10-phenanthroline,bpy?=?2, 2′bipyridine,dmb?=?4, 4′-dimethyl-2, 2′-bipyridine] have been synthesized and characterized by elemental analysis, IR, UV–Vis, 1H& 13C NMR and mass spectra. The DNA-binding properties of the Ruthenium(II) complexes were investigated by spectrophotometric methods, viscosity measurements and light switch studies. These three complexes have been focused on photo activated cleavage studies with pBR-322 and antimicrobial studies. Experimental results indicate that the three complexes intercalate into DNA base pairs and follows the order of 1?>?2?>?3 respectively. Molecular docking studies also support the DNA interactions with complexes through hydrogen bonding and vander Waal’s interactions. Cytotoxicity studies with Hela cell lines has been revealing about anti tumor activity of these complexes.  相似文献   

20.
合成了一系列异核配合物[(Eu1-xPrx)(DPSO)(phen)3(ClO4)2]ClO4*nH2O(x=0.000~0.200, DPSO为二苯亚砜, phen为邻菲罗啉, n=1, 2, 3, 4, 5, 6). 对配合物进行了组成分析, 摩尔电导, IR光谱及荧光激发和发射光谱的测定. 荧光光谱测定结果表明 本身不发光的Pr3+对Eu3+的发光产生较大影响, 当Pr3+的掺入量为0.001 mol时, 可使Eu3+的发光强度提高到200%以上,随着Pr3+浓度的增大,对Eu3+的发光先敏化后猝灭.Eu3+在配合物中所处的格位对称性低,无对称中心.  相似文献   

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