用斜入射光反射差法无标记实时监测不同浓度兔IgG和山羊抗兔IgG反应的动力学过程

用斜入射光反射差法无标记实时监测1.25 mg/mL, 2.50 mg/mL和5.00 mg/mL三种不同浓度兔IgG和浓度为0.02 mg/mL山羊抗兔IgG反应的动态过程, 同时监测和获得三种不同浓度IgG反应的动力学曲线以及相应的反应时间. 实验结果表明, 斜入射光反射差法不仅能无标记地判别生物分子之间是否发生反应, 而且能无标记实时监测生物分子反应的动力学过程, 在生物分子相互作用的研究方面具有潜在和广泛的应用前景.     

Detection of protein microarrays by oblique-incidence reflectivity difference technique

Biological microarrays with different proteins and different protein concentrations are detected without external labeling by an oblique-incidence reflectivity difference (OIRD) technique. The initial experiment results reveal that the intensities of OIRD signals can distinguish the different proteins and concentrations of protein. The OIRD technique promises feasible applications to life sciences for label-free and high-throughput detection.     

Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method

Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots.Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the obliqueincidence reflectivity difference(OIRD)method.We obtained the reaction results and the reaction dynamic curves of 500 protein dots.In addition,we also used label-free detection of protein microarrays of 10080 sample dots,including BSA and different concentrations of Mouse IgG and Rabbit IgG,by OIRD.The obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported herein.Experimental results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules.     

Sensitivity investigation of the different direction vibrations of a SNOM probe

In this article, we analyze the sensitivity of lateral, axial, and torsional vibration of a scanning near-field optical microscope (SNOM) probe. An exact solution for the title problem is obtained using the theory of beam and torsional vibrations. Sensitivities are obtained for a wide range of the intervening physical parameters. This paper discusses the relationship between the sensitivity of a SNOM probe and its geometry. The analysis is presented for a probe in three different cases lateral vibration, axial vibration, and torsional vibration, for a fixed-free configuration. To derive the mathematical relation between the probe and sample (by utilizing springs and damping to simulate different environments) through the process of theory analysis for different environments. Thus a universal touchless SNOM probe sensitivity controller is developed for appropriate control and adjustment of the probe sensitivity suitable under different environments. Such controller helps the SNOM probe achieve the nanoscale resolution through proper control and adjustment probe sensitivity in accordance to changes to different environments and reduce the chance of being damaged during the scan, which in the end further advances the sample inspection technique.     

Probe Indentation: A Mesoscale Approach to Characterise Powder Systems: Experimental Investigation of Monomodel and Bimodal Diameter Distributions of Glass Spheres

An experimental study investigating a novel power characterisation method is investigated. The scope of this experimental work is to assess the feasibility, suitability and sensitivity of small scale probe indentation as a mechanism by which Discrete element method (DEM) may be calibrated. This meso–scale approach is chosen for investigation since it bridges the gap between the single‐particle methodologies of atomic force microscopy (AFM) and bulk measurements such as shear cell testing. Five different mono‐modal populations of glass bead and bi‐modal blends of these populations have been examined. The force‐displacement profile from a spherical probe was measured during indentation into a powder bed. Discernable differences in the resultant force‐profile are quantifiable and a mechanism has been proposed for the physical basis of these different characteristics.     

高能超声制备碳纳米管增强AZ91D复合材料的声场模拟

建立了高能超声制备碳纳米管增强AZ91D复合材料的声场计算模型, 并采用有限元方法计算了20 kHz超声直接作用下AZ91D熔体的声场分布, 熔体声场呈辐射状分布, 距离声源越远, 声压幅值越低. 采用超声作用下单一气泡变化模型描述超声作用下AZ91D 熔体中的空化效应, 通过对Rayleigh-Plesset方程的求解, 得到了不同声压作用下气泡的变化规律, 获得了声压幅值与熔体空化效应的关系, 声压幅值越大, 气泡溃灭半径阈值越小, 熔体发生空化效应越容易. 计算了固定坩埚尺寸、不同超声探头没入熔体深度情况下的声场, 得到了超声探头最优没入深度为30 mm左右. 将声场计算结果以及AZ91D熔体中空化效应的发生规律进行综合分析, 得到了超声功率对有效空化区域的影响规律, 超声功率较大时, 有效空化区域体积随超声功率近似成线性增大. 最后, 通过甘油水溶液超声处理实验, 验证了模拟计算的准确性.     

HR/MAS高分辨魔角微量探头用于天然橡胶固体1H、13C NMR测试及其与液体BBO探头、固体CP/MAS探头测试的比较

采用BRUKER高分辨魔角微量探头（HR/MAS），液相宽带BBO探头和固体CP/MAS探头，对天然橡胶固体、乳液以及天然橡胶溶于氘代苯的溶液进行了¹H、¹³C 1D和2D NMR谱的测试和比较. 发现HR/MAS探头用于天然橡胶固体和乳液时可以得到高分辨的¹H、¹³C谱，克服了CP/MAS探头测试固体¹³C NMR谱或者是固体¹H NMR谱时，谱图存在S/N值可能较小、谱峰可能宽化的弱点.     

镀纳米结构薄膜光纤探针的光子隧道扫描显微镜成像数值模拟

利用时域有限差分法,对镀纳米结构薄膜光纤探针的成像特性进行了三维数值模拟。在考虑光纤和样品相互作用的基础上,采用等效入射波法设置入射波和等高扫描。从成像分辨率和灵敏度的角度比较了不同纳米颗粒与样品间距、纳米颗粒大小、排列方式以及排列密度对成像的影响。结果表明,镀纳米结构薄膜光纤探针的光子隧道显微镜无论在制备的可行性上,还是在分辨率和灵敏度上都有显著的改进。     

静动结合的化学腐蚀法制备近场光学探针的理论分析

理论上分析了静动结合的化学腐蚀法制备探针的具体机理及过程。在静态腐蚀的过程中, 利用流体力学Young-Laplace方程的一级近似解获得了光纤插入到HF酸中形成的新月形高度。在动态腐蚀过程中, 详细分析了当静态腐蚀时间和动态腐蚀时间分别取不同值时, 光纤移动速度对光纤探针结构的影响。利用此法可制备出尖端锐利、大锥角或多锥体等各种结构的光纤探针。这为实验上制备出性能优良的探针, 为拓宽扫描近场光学显微镜的应用范围奠定基础。将上述理论分析的结果与本文实验中所得初步结果进行了比较, 所得结果一致。     

Study of plasma—solid interaction in electronegative gas mixtures

Low-temperature reactive plasmas employing electronegative gases are often used in modern technologies. Negative ions in such plasmas affect the transport of charged species and in this way influence the processes in the boundary layer between plasma and surface of metal substrate or probe. The contribution presents results of the computer experiment describing the interaction of electronegative plasma with immersed substrates. The method of solution was the particle simulation technique and several approximations were used; the most important was the simplified geometry of substrates. The simulation is based on experimental data obtained in a dc glow discharge in mixtures of oxygen with rare gases. This work is a part of the research plan MSM0021620834 that is financed by the Ministry of Education of Czech Republic.