共查询到19条相似文献,搜索用时 109 毫秒
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《中国光学与应用光学文摘》2006,(2)
眼镜、放大镜、显微镜、望远镜TH742.652006021689高斯光束荧光共焦显微镜的三维光学传递函数=Opticaltransfer function of a fluorescent confocal microscope withextended Gaussian source[刊,中]/杨初平(华南农业大学理学院.广东,广州(510642))∥激光技术.—2005,29(5).—552-554研究高斯光源的束斑半径、光源孔径和探测器孔径对荧光共焦显微镜3-D OTF的影响,获得了具有有限光源孔径、探测器孔径和束斑半径的荧光共焦显微镜的三维光学传递函数(3-D OTF)。数值计算结果表明,束斑半径影响到光源孔径和探测器孔径的选择,与采用平行光… 相似文献
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荧光共焦扫描系统成像特性的优化 总被引:8,自引:0,他引:8
对荧光共焦扫描系统用光强点扩散函数进行傅里变换得到系统三维传递函数的数学模型,并由此求得环形透镜和各种有限大小探测器系统的光学传递函数。用计算机模拟和光学传递九数值计算,分析了采用不同环形透镜及探测器对系统成像特性的影响 相似文献
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在研制用于对厚的生物样品进行光学断层成像的共焦扫描荧光显微镜时,由于成像信号十分微弱及存在很强的多次散射作用,因此杂散光的抑制非常重要,而信噪比、信号背景比就成为决定能否获得高对比度、高分率图像的关键。运用光学信息量的概念,在已有的光学成像系统信息量计算、共焦扫描荧光显微镜信噪比及传递函数计算的基础上,详细分析了共焦扫描荧光显微镜信息量与信噪比等之间的定量关系。该关系表明,为了充分利用共焦扫描荧光显微镜的成像性能,必须选择适当的探测小孔。所得的结果对于共焦扫描荧光显微成像系统的研制有重要的实用价值。 相似文献
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共焦激光扫描显微镜技术进展 总被引:5,自引:0,他引:5
共焦激光扫描显微镜已延伸到光学显微术领域,包括共焦荧光显微镜、共焦扫描光纤显微镜、彩色共焦激光显微镜、共焦光栅光学显微镜等。并很快成为半导体材料检测和生物医学诊断的重要工具。本文对国外该显微镜技术的特性和新的进展进行了综述。 相似文献
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对自身不发荧光且不便于荧光标记的化学或生物学样品,集相干反斯托克斯拉曼散射与激光共焦扫描显微镜于一身的相干反斯托克斯拉曼散射显微镜是一种好的选择。因为相干反斯托克斯拉曼散射是一种非线性过程,相干反斯托克斯拉曼散射显微镜的显微成像特性与一般的共焦显微镜非常不同。首先计算了焦点附近相干反斯托克斯拉曼散射激发场的偏振分布,然后,利用格林函数方法,得到了以赫兹偶极子为源的波动方程的精确解,发现对于不同的成像配置和样品形状,像场的相干反斯托克斯拉曼散射场分布非常不同,因此传统的显微镜成像表征方式(如点扩展函数)将不再能描述相干反斯托克斯拉曼散射显微镜的成像特性。 相似文献
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Sampling in fluorescence microscopy is treated using the concept of the three-dimensional (3D) optical transfer function (OTF). The border of the OTF frequency surface defines the required minimum sampling. The shape of the OTF is derived from simple considerations and valid for far-field high numerical aperture, vector theory. Optimal regular sampling is achieved by a hexagonal grid in 2D, and corresponding hexagonal structures, body-centered cubic (bcc) and hexagonal close-packed (hcp) structures, in 3D. As compared to standard (rectilinear grid) sampling a reduction of 13.4% in 2D and 29.3% in 3D can be achieved with optimized sampling. This reduction in data size is also accompanied by an imaging speed improvement, a reduction of sample bleaching, and can lead to imaging with better signal to noise ratio. 相似文献
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Systems in which the point spread function (PSF) is a rotating beam have increasing use in three-dimensional (3D) microscopy and depth estimation. We analyze in several ways the 3D optical transfer function (OTF) of Gauss Laguerre modes and rotating beams. This is based on analysis of 3D OTFs of general aperture functions. Consequently, we suggest a criterion for depth resolution based on an effective cutoff of the axial frequency response. This criterion can be used to optimize PSFs explicitly and directly, to maximize axial resolution. 相似文献
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提出一种用非相干光系统识别彩色图像的方法,将脸色图像分解成三个单色像处理,设计一可实现的仅相位光学传递函数,对其进行带能优化,人而提高信噪比并使相关峰锐化,通过一对滤波器来合成该光学传递函数,获得了采色图像相关识别的实验结果。 相似文献
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Mingkai Liu 《Optik》2010,121(21):1966-1975
Optical transfer function (OTF) and image spectrums of the layered metal (M)-dielectric (D) superlenses (MD)n (where n is the number of cycles) are analyzed via transfer matrix method and effective-medium model. It is shown that the magnitude of OTF (MTF) with a high cutoff wave vector does not guarantee high resolution; the fluctuation of phase of OTF (PTF) is also a prime factor causing superlens imaging distortion. When the layered metal-dielectric superlenses have a small number of cycles, we find that the different types of superlenses (such as (MD)nM, (MD)n, and (DM)nD) with the same effective permittivity have totally different performances; the superlens with (DM)nD arrangement has the best performance; this will be valuable for designing layered metal-dielectric superlenses with less cycles. 相似文献
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Solving the atmospheric scattering optical transfer function using the multi-coupled single scattering method 下载免费PDF全文
The atmospheric scattering optical transfer function (OTF) is solved by applying the multi-coupled single scattering (MCSS) method to the three-dimensional radiative transfer equation (RTE) under the periodic ground condition. This approach is a direct hit to the atmospheric scattering OTF using the same original context of modulation transfer function (MTF) measurement, i.e., images of sinusoidal grating at different spatial frequencies. Both the amplitude and phase shift of the OTF at various zenith and azimuth angles can be obtained at an arbitrary spatial frequency. 相似文献
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Through the one-dimensional analysis of the diffraction of a phase grating, we calculated the optical transfer function (OTF)
of a phase grating. By a 0th-order approximation of the optical transfer function, we analyzed the feasibility of using it
as a spatial frequency filter and obtained the structure of a grating for the optimum-filtering characteristics. The OTF of
a phase grating as an optical low-pass filter is optimal when the incoming light is equally divided into three beams. The
distance of those beams must be equal to the pixel size. The theoretical development was verified through an experiment.
Received: 21 May 2001 / Revised version: 16 July 2001 / Published online: 15 October 2001 相似文献