首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 46 毫秒
1.
李莹  杜虹  赵常志 《光谱实验室》2010,27(4):1498-1502
通过电化学氧化肾上腺素,使其生成羟基吲哚类荧光物质(λem=490nm),实现了药物中肾上腺素含量的电化学氧化-荧光检测。研究结果表明,荧光强度与肾上腺素浓度分别在2.00×10-7—1.00×10-6mol/L和1.00×10-6—4.00×10-5mol/L范围内呈良好的线性关系,检出限为6.70×10-8mol/L。该方法用于药物制剂中肾上腺素含量的测定,相对标准偏差小于1.88%(n=7),加标回收率为88.4%—116.6%。  相似文献   

2.
基于铁(Ⅲ)对荧光试剂邻苯二酚的荧光熄灭,建立了测定微量铁的荧光分析方法.在0.3mol/L的HCl介质中,最大激发与发射波长分别为277nm和313nm,铁的荧光强度与浓度呈良好的线性关系,测定铁浓度的线性范围为1.59×10-5-1.26×10-3mol/L,检出限为7.14×10-6mol/L,常见的共存离子不干扰测定.本方法用于环境水中铁(Ⅲ)的测定,结果满意.  相似文献   

3.
铜-氧氟沙星荧光体系测定氧氟沙星   总被引:1,自引:0,他引:1  
采用荧光分光光度法研究了Cu2+与氧氟沙星配合物的荧光光谱特性.Cu2+在pH=5.0的HAc-NaAc缓冲溶液中能使氧氟沙星的荧光猝灭,且荧光强度降低值△F和氧氟沙星浓度在一定范围内呈良好的线性关系.据此建立了一种以Cu2+为荧光探针测定氧氟沙星的新方法,该方法线性范围为1×10-7-2×10-6mol/L,检出限为2.165×10-8mol/L.用于氧氟沙星片剂和鸡肉中药物含量的测定,结果令人满意.  相似文献   

4.
建立了荧光光谱法测定合成色素亮蓝的新方法.亮蓝在水溶液中有很好的荧光效率,以397.0nm为激发波长,456.0nm为发射波长测定亮蓝的荧光强度,在5.00×10-7-2.00×10-5mol/L浓度范围内,亮蓝的荧光强度与其浓度呈线性关系,线性回归方程为F(%)=2.82×106C(mol/L)+ 20.6(相关系数r=0.9943),检出限0.396μg/mL.利用该方法测定了两种市售饮料中的亮蓝,加标回收率在98.6%-110.5%之间.  相似文献   

5.
石墨烯量子点荧光探针测定肾上腺色腙   总被引:6,自引:6,他引:0       下载免费PDF全文
马红燕  王艳妮 《发光学报》2016,37(2):230-236
通过高温裂解柠檬酸合成了水溶性石墨烯量子点(GQDs),并应用钝化剂PEG2000进行修饰,提高了GQDs的量子产率。应用荧光光谱、紫外-可见光谱、红外光谱对其发光特性进行了研究,测定了荧光寿命。实验发现,在p H=7.40的Tris-HCl缓冲液中,肾上腺色腙(CBZC)对GQDs荧光强度有明显的猝灭作用。基于此提出了以GQDs为探针测定肾上腺色腙的新方法。实验考察了缓冲溶液用量、缓冲溶液种类、量子点浓度、反应时间以及表面活性剂等多种因素对反应体系的影响。当量子点浓度为2.3×10-3mol/L时,肾上腺色腙浓度在4.0×10-7~1.2×10-5mol/L(0.995 6)范围内与荧光猝灭值ΔF呈良好的线性关系,方法检出限为1.5×10-7mol/L,相对标准偏差为0.15%(n=5,c=4.0×10-6mol/L)。该方法对于样品中肾上腺色腙含量测定的回收率为97.46%~101.6%。通过测定温度对猝灭常数的影响以及紫外-可见吸收光谱的变化确定了二者的猝灭过程和相互作用力类型。  相似文献   

6.
在酸性介质中.高锰酸钾可氧化辛弗林产生化学发光,对影响化学发光强度的因素进行了实验和探讨,建立了高锰酸钾化学发光体系测定辛弗林的新方法.在最佳实验条件下,辛弗林的线性范围为4.00×10-7-2.00×10-5mol/L检出限为6.96×10-mol/L.该体系已经成功应用于枳壳中生物碱含量的测定,回收率为92.8%.此外,本文还测定了酸性高锰酸钾-辛弗林体系的化学发光光谱和荧光光谱,初步探讨了化学发光反应机理.  相似文献   

7.
酶催化荧光光谱法测定盐酸多巴胺   总被引:1,自引:0,他引:1  
利用盐酸多巴胺对血红蛋白酶催化荧光体系的猝灭作用,建立了酶催化荧光光谱法测定盐酸多巴胺的新方法.研究了该猝灭体系的最佳实验条件及动力学行为,测定的线性范围为2.1×10-8--8.4×10-4mol/L,检出限为8.2×10-9mol/L.对浓度为5.2×10-6mol/L的盐酸多巴胺进行11次平行测定,其相对标准偏差...  相似文献   

8.
基于镁(Ⅱ)对恩诺沙星的荧光强度有显著的增敏作用,建立了镁(Ⅱ)增敏,荧光光度法快速测定恩诺沙星的新方法.在pH5.5的酒石酸-酒石酸钠缓冲溶液中,恩诺沙星在1.00×10-8-1.00×10-6mol/L范围内符合比耳定律,方法检出限为7.5×10-10mol/L.已用于片剂中恩诺沙星的测定,样品平均回收率为93.91%,RSD=0.55%.实验表明,该方法简单、快速、灵敏、准确.  相似文献   

9.
在pH=5.00的HAc-NaAc介质中,铽(Ⅲ)与甲苯磺酸妥舒沙星发生络合,产生稀土敏化荧光现象,使铽(Ⅲ)的λem=545nm处荧光增强最为显著,当适量叶酸加入时,铽(Ⅲ)的荧光强度明显下降,以此建立了简单、快速、灵敏的测定叶酸的荧光分析方法。叶酸溶液在7.3×10-5—5.6×10-8mol/L浓度范围内符合线性关系,方法检出限为8.1×10-9mol/L。该法用于叶酸片剂中叶酸含量的测定,其回收率为98.4%—102.9%。  相似文献   

10.
以荧光衍生光谱法研究了氯化钡与玫红酸形成络合物的荧光光谱,建立了钡离子的荧光光谱分析方法,在优化条件下:溶液pH=11.2,反应时间为5min,乙醇与水溶剂的比为15%.在2.5×10-11-5×10-5mol/L范围内,络合物的荧光强度与钡离子浓度存在两段线性关系,检出限为2.5×10-11mol/L.方法用于钡餐在水中溶解度的测定,结果与文献报道值一致.  相似文献   

11.
Two-dimensional scanning of a 0.6328 mum guided-light beam has been realized using noncolinear acousto-optic (AO) coplanar Bragg diffraction together with colinear AO guided-mode to substrate radiation-mode conversion in a Z-cut Xpropagation LiNbO3 proton-exchanged (PE) waveguide. The two surface acoustic (SAW) waves utilized are at the center frequencies of 500 and 200 MHz, propagating in the Y and X axes, respectively. Two-dimensional scanning of approximately 720 resolvable light beam spots, namely, 18 40 (horizontal vertical) scanning, has been demonstrated using a light beam of 1.0 mm aperture. The total number of resolvable beam spots can be greatly increased from 720 by simply utilizing SAW transducers of larger bandwidth and a light beam of greater aperture. It should also be possible to significantly increase the diffraction efficiency from 3 % by optimizing the parameters of the PE waveguide and the SAWs.  相似文献   

12.
A simple technique of pulse compression, based on the linear chirp compensation of self-phase modulation (SPM) spectra in dispersion shifted fibers, is demonstrated. The optimization procedure is carried out, for a short span of a single-mode fiber, using a parabolic law, which describes the behavior of the squared output pulse width versus the pump peak power in the case of Gaussian pulses. The experimental results give a minimum pulse duration of 233 fs, which is in good agreement with the model. Shorter and coherent pulses, down to 90 fs, have been obtained by inserting an interference filter at the optical output.  相似文献   

13.
The Shanghai Synchrotron Radiation Facility(SSRF)booster ring,a full energy injector for the storage ring,is deigned to accelerate the electron beam energy from 150MeV to 3.5GeV that demands high extraction efficiency at the extraction energy with low beam loss rate when electrons are ramping.Closed orbit distortion(COD)caused by bending magnet field uniformity errors which affects the machine performance harmfully could be effectively reduced by bending magnet location sorting.Considering the affections of random errors in measurement,both ideal sorting and realistic sorting are studied based on measured bending magnet field uniformity errors and one reasonable combination of bending magnets which can reduce the horizontal COD by a factor of 5is given as the final installation sequence of the booster bending magnets in this paper.  相似文献   

14.
15.
A review of schemes for multiple access in fiber optic networks shows that a hybrid of wavelength and code division multiple access (WCDMA) combines the best features of both. In particular, the hybrid scheme retains the large information carrying capacity of wavelength division multiple access (WDMA) and flexibility of code division multiple access (CDMA). In this paper WDMA, optical CDMA (OCDMA), and WCDMA networks are discussed. In OCDMA networks, concept of incoherent and coherent coding including inverse decoding and matched filter is introduced. The delay performance of both WDMA and WCDMA networks, under the simple suboptimum access protocols based on cyclic search, is computed. It has been shown quantitatively that tuning delay significantly affects the delay performance of both WDMA and WCDMA networks. Futhermore, delay performance of WCDMA networks is always better than the WDMA networks for the same tuning delay, load, and number of users.  相似文献   

16.
Results of a study of the effect of γ-radiation on the spectral luminescence properties of cadmium tungstate crystals doped with silver, bismuth, and molybdenum cations are presented. Spectral characteristics of the nondnnnoped crystals are briefly described. Absorption and photo and X-ray luminescence spectra of the crystals taken before and after exposure to γ-radiation (5.5·104 Gy) are compared. It is found that the spectral characteristics of the crystals doped with silver, bismuth, and molybdenum cations do not change markedly after the exposure. The relation between the type of impurity-induced defects, individual characteristics of the impurity cations, and the character of the effect of γ-radiation on the spectral luminescence properties of impure crystals is analyzed (preliminarily). Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 64, No. 1, pp. 55–60, January–February, 1997.  相似文献   

17.
A transient suppression method for piezoelectric ceramic transducer is presented. By adding some special pedestal pulses to the original transmitted signal, the transient effects in the output of the transducer can be just precisely transmission by use of a narrowband transducer. canceled, thereby achieving the broadband The experimental results show that the transient responses of the sinusoid pulses and coded signals are suppressed successfully and the acoustic waveforms radiated into the surrounding water are improved significantly.  相似文献   

18.
We experimentally demonstrate amplitude squeezed soliton utilizing intensity-dependent self-phase modulation in an asymmetric Sagnac interferometer.The system.whose components are connected via ferrule connector/physical connection(FC/PC)fiber connectors,constitutes all-fiber configuration to generate squeezed soliton.Soliton amplitude reduction measured by homodyne detection is near 4.0 dB below the shot-noise level.Optimal squeezing fields in both simple and compact all-fiber configuration are obtained.  相似文献   

19.
The(2 1)-dimensional nonlinear Schr(o)dinger(NLS)equation with spatially inhomogeneous nonlinearities is investigated,which describes propagation of light in(2 1)-dimensional nonlinear optical media with inhomogeneous nonlinearities.New types of optical modes and nonlinear effects in optical media are presented numerically.The results reveal that the regular split of beam can be obtained in (2 1)-dimensional nonlinear optical media with inhomogeneous nonlinearities,by adjusting the guiding parameter.Furthermore,the stability of beam regular split is discussed numerically,and the results reveal that the beam regular split is stable to the finite initial perturbations.  相似文献   

20.
In the real-world application of face recognition system, owing to the difficulties of collecting samples or storage space of systems, only one sample image per person is stored in the system, which is so-called one sample per person problem. Moreover, pose and illumination have impact on recognition performance. We propose a novel pose and illumination robust algorithm for face recognition with a single training image per person to solve the above limitations. Experimental results show that the proposed algorithm is an efficient and practical approach for face recognition.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号