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基于荧光响应的氨传感膜研究 总被引:2,自引:1,他引:1
利用有机改性溶胶-凝胶的包埋方式,以氨基荧光素(AF)为荧光指示探针制备氨传感敏感膜,考察了AF与γ-[(2,3)-环氧丙氧基]丙基三甲氧基硅烷(GOPS)作用后的膜泄漏度及荧光光谱行为的变化,对氨传感敏感膜的配比进行了一系列的优化,获得了对氨具有高响应灵敏度而对pH无响应的氨光传感敏感膜.利用自行构建的氨传感检测装置对水体中氨进行了检测,传感膜对水溶液NH4的最低检出限为0.3 μg·mL-1,测定的相对标准偏差为4%,线性范围为1~80μg·mL-1,响应时间(t95)为4 min,敏感膜使用寿命大于6个月. 相似文献
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基于钌配合物荧光猝灭氧二次传感BOD检测的研究 总被引:2,自引:0,他引:2
考察了利用溶胶-凝胶基质制备的氧荧光传感膜的光谱性质,实验结果表明,溶胶-凝胶基质与Ru(dpp)3(ClO4)2配合物的基态之间不存在较强的相互作用,具有较好的透光性.将氧光化学传感器作为二次传感,制备了响应良好的光化学BOD微生物传感器,研究结果表明这种传感器对于较低浓度的BOD含量有着良好的线性范围,并且其响应活性保持良好,具有较长的使用寿命和良好的稳定性. 相似文献
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利用四甲氧基硅烷(TMOS)、二甲基二甲氧基硅烷(DiMeDMOS)与聚乙烯醇(PVA)构成的有机改性材料分别包埋五种不同淡水耗氧菌,制备光化学生物耗氧量(BOD)的传感敏感膜,并在自行构建的BOD测定仪上考察其光谱响应行为.实验结果表明,分别包埋五种淡水耗氧菌和它们混合菌种的BOD传感膜对BOD的荧光强度响应范围为0~60 mg·L-1至0~120 mg·L-1,线性相关系数为0.976~0.997,响应时间为0.5~8.7 min,传感敏感膜可连续使用30 d以上.实验研究考察了不同实验条件下如温度和测定体系的pH对BOD传感膜响应的影响.实验结果发现,在4℃下保存12个月后,BOD传感膜仍保持原有响应值的85%以上,显示出较好的重复性和稳定性.初步应用结果表明,BOD传感膜可应用于地表水中的BOD检测. 相似文献
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钌(Ⅱ)配合物有机改性溶胶-凝胶氧传感膜荧光行为的研究 总被引:1,自引:0,他引:1
本文考察了利用甲基硅氧烷(TMOS)和二甲基二甲氧基硅烷(DiMe-DMOS)为共先驱体制备的有机改性溶胶-凝胶(sol-gel)氧荧光繁感传感膜,比较了5种不同配体的Ru配合物在不同极性溶剂和sol-gel膜内的荧光行为,考察了膜极性的改变对Ru化合物荧光光谱的影响,以及氧对不同极性有机改性sol-gel包埋Ru化合物荧光猝灭的程度。研究结果表明,在sol-gel膜内,Ru化合物的荧光寿命都有不同程度的增加,同时对氧的猝灭程度增加。另外膜的极性对氧猝灭的程度也有很大的影响,增加膜的疏水性,将提高传感膜对氧浓度的响应。 相似文献
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使用四甲氧基硅烷(TMOS)和二甲基二甲氧基硅烷(DiMe-DMOS)为共先驱体,采用溶胶-凝胶的有机掺杂,制备对pH具有宽程响应的敏感膜。详细考察了包埋溴酚蓝和溴酚绿的敏感膜对pH的响应值、响应时间、泄漏和可逆性等响应性能指标,并进行了溴酚蓝和溴酚绿在水相与膜内吸收光谱的研究。 相似文献
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氧对钌(Ⅱ)配合物溶胶-凝胶膜荧光响应的影响 总被引:3,自引:0,他引:3
利用蓝光LED(λmax =4 6 0nm )构建的氧荧光猝灭检测系统 ,考察了不同Ru配合物以甲基硅氧烷(TMOS)和二甲基二甲氧基硅烷 (DiMe DMOS)为共先驱体 ,制备的有机改性溶胶 凝胶 (sol gel)膜内荧光猝灭行为。DiMe DMOS的加入量会影响敏感膜的极性 ,从而影响敏感膜对氧浓度的响应。利用自行构建的装置对水体中氧进行了检测 ,传感膜对氧饱和水溶液测定的相对标准偏差为 1 12 % (n =6 ) ,响应时间为 30s,对氮饱和水溶液测定相对标准偏差为 0 39% (n =6 ) ,响应时间为 90s,敏感膜使用寿命大于 6个月。 相似文献
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BOD光化学传感敏感膜荧光响应的研究 总被引:1,自引:1,他引:0
利用自行构建的BOD光纤传感装置对海水中BOD含量进行了检测,考察了海洋耗氧菌种在四甲 基硅氧烷(TMOS)、二甲基二甲氧基硅烷(DiMe-DMOS)和聚乙烯醇(PVA)包埋情况下,对BOD的荧光响应 行为以及耗氧菌种的加入量对BOD浓度的响应情况。传感膜对5 mg·L-1 BOD溶液测定的相对标准偏差为 1.2%(n=6),响应时间为3.2 min,敏感膜使用寿命大于12个月。实际海水样品检测表明,利用BOD光纤 传感检测的结果与国标BOD5方法,存在较好的一致性。 相似文献
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JIANG Wei-na YANG Shi-long LU Wen XU Li TANG Ying XUE Hua-yu GAO Bu-hong DU Li-ting SUN Hai-jun MA Meng-tao XU Hai-jun CAO Fu-liang 《光谱学与光谱分析》2018,38(8):2650-2656
制备了一种基于天然产物槲皮素接枝硅包银核壳结构的纳米荧光传感器(Ag@SiO2@Qc),对铜离子具有好的选择性和灵敏性。Ag@SiO2@Qc与Cu2+离子结合后,荧光发射强度发生猝灭,并且可通过荧光滴定光谱得到了荧光滴定曲线:y = -32.864x+587.59(R2=0.998),其线性范围分别为:3×10-7~4.8×10-6 mol·L-1,最低检测限为1.0×10-7 mol·L-1。并且将Ag@SiO2@Qc应用于环境中水样的检测结果的准确度好,精密度高,而且更加环保、方便、快捷,具有很大发展潜力与应用价值。 相似文献
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A method to determine glucose using an optical sensor prepared by entrapping glucose oxidase into silica sol-gel column has been developed. The silica sol-gel film was coated on alumina substrate. The optical sensor is based on the chemiluminescence intensity from the reaction of periodate and hydrogen peroxide in K2CO3 medium. The effect of the ratio of water and alcohol for the preparation of TEOS sol on chemiluminescence intensity was investigated. The effects of pH of enzyme reactor, concentrations of potassium periodate and SDS, and flow rate on the chemiluminescence intensity were studied to find the optimum experimental conditions to determine glucose. The chemiluminescence intensity increased linearly with increasing glucose concentration from 5.0 x 10(-4) M to 1.0 x 10(-7) M and the detection limit was 4.0 x 10(-8) M. Interference effects from some metal ions on chemiluminescence intensity were also investigated. 相似文献
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将1-(2-吡啶偶氮)-2-萘酚(PAN)和荧光试剂罗丹明B(RB)联合作为指示剂,再通过碱性催化的溶胶凝胶法将指示剂固定在与传像光纤相联结的自聚焦透镜端面上,成功制备了一种具有传像功能的荧光锌离子传感探头.与改进的金相显微镜和激光器相联接,可同时实现对锌离子浓度的检测和样品外貌的观测.此系统在锌离子浓度为0.1~1 ... 相似文献
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A very sensitive and reversible optical chemical sensor based on a novel tetradentate Schiff base namely N.N/bis(2-aminothiophenol)benzene-1,2-dicarboxaldehyde (ATBD) immobilized within a plasticized PVC film for Hg2+ determination is described. At optimum conditions (i.e. pH 6.0), the proposed sensor displayed a linear response to Hg2+ over 1.0?×?10?10 ? 1.0?×?10?2 mol L?1 with a limit of detection of 7.23?×?10?11 mol L?1 (0.0145 μgL?1). Moreover, the results revealed that, under batch condition, the sensor is fully reversible within a response time?~?35 s. In addition to its high stability and reproducibility, the sensor showed good selectivity towards Hg2+ ion with respect to common metal cations. The sensor was successfully applied for determination of Hg2+ ion in some real samples, including hair, urine and well water samples. The results were in good correlation with the data obtained using cold vapor atomic absorption spectrometry. 相似文献
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A new OFF-ON fluorescent chemosensor (H1) composed of a naphthalimide fluorophore and a 6-[(quinolin-8-yloxy)methyl]pyridin-2-ylmethanamine receptor has been synthesized and characterized by infra-red, (1)H NMR, (13)C NMR and mass spectrometry. The developed chemosensor H1 exhibited good turn-on and reversible responses toward Hg(2+), with excellent selectivity and sensitivity, in a neutral buffered aqueous solution. Other common metal ions did not interfere with the fluorescence-enhancement response to Hg(2+). Furthermore, the chemosensor H1, at a concentration of 10?μM, showed a rapid and linear response toward Hg(2+) in the concentration range 0-10?μM. On addition of 10?μM Hg(2+), the fluorescence intensity of H1 was enhanced about 4-fold. The detection limit was calculated to be 63?nM. The association constant was 1.11?×?10(5)?M(-1). The fluorescence quantum yield and lifetime of H1/Hg(2+) were 0.42 and 3.83?ns, respectively. 相似文献
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Shaojun Huang Ping Du Chungang Min Yaozu Liao Hui Sun Yubo Jiang 《Journal of fluorescence》2013,23(4):621-627
Poly(1-amino-5-chloroanthraquinone) (PACA) was firstly synthesized by a chemically oxidative interfacial polymerization. The PACA has been developed as a fluorescent sensor for the determination of Fe(III) in semi-aqueous solution at pH 7.0. The sensor exhibited remarkably high sensitivity toward Fe3+ since the fluorescence of the polymer could be significantly quenched even though trace Fe3+ was added. The sensor showed a linear fluorescence emission response over a wide concentration range from 1.0?×?10?10 to 1.0?×?10?4 M, with an ultra-low detection limit of 2.0?×?10?11 M. The quenching of the fluorescence was found to be static one due to the formation of non-fluorescent complex in the ground state. 相似文献
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Ayman A. Abdel Aziz Rania G. Mohamed Fatma M. Elantabli Samir M. El-Medani 《Journal of fluorescence》2016,26(6):1927-1938
A novel fluorimetric optode has been developed for the highly selective and sensitive for the determination of ultra trace amounts of Al3+ ions. The proposed fluorescent optode is based on the incorporation of a simple and effective fluorescent sensor tridentate NOS Schiff base N-(2-hydroxynaphthylidene)-2-aminothiophenol (H2L) in a plasticized PVC containing KTpClPB as a lipophilic anionic additive. H2L was synthesized by a facile one-step Schiff base reaction. The plasticized PVC-membrane displays a calibration response for Al3+ ions over a wide concentration range from 1.0 × 10?9 to 4.4 × 10?3 mol/L. The fluorescence signal of the optode membrane can be easily recovered by immersion in 0.01 M EDTA. In addition to high stability and reproducibility, the sensor shows a unique selectivity towards Al3+ ion with respect to common co-existing cations, particularly Ga3+and In3+. The proposed optode was applied successfully for determination of Al3+ in some real samples, including bottled drinking waters, bottled mineral waters and soft drinks. 相似文献
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以铽离子为荧光探针测定尿样中痕量环丙氟哌酸 总被引:3,自引:0,他引:3
详细研究了环丙氟哌酸同稀土离子铽形成的配合物体系的荧光光谱特性及实验条件对荧光强度的影响。在pH为6.0的条件下,配合物荧光体系可发射铽离子强的特征荧光,其最大激发和发射波长为328和545nm。以稀土离子铽为荧光探针,测定尿样中痕量的环丙氟派酸可获得满意结果。 相似文献
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研究了一种使用超声波辅助提取(UAE)-原子荧光法测定茶叶痕量汞的快速方法.采用Plackett-Burman设计从6个影响汞提取率的因素中筛选出3个显著因素—超声时间St,超声温度T和HNO3:H2O2(1:1,δ)体积A2,并采用中心组合设计和响应面法优化参数.结果表明,最优提取条件为,浸泡时间6 min,St 8... 相似文献
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利用丝网印刷技术制备了基于Ru(bpy)23 (钌联吡啶)的印刷电极电致化学发光传感器,这种传感器具有制作简单、成本低、重现性好、对草酸盐的响应范围宽、检测限低等优点。详细研究了电极的制作方法以及发光试剂的固定化。在最优条件下,在pH 6.0的0.2 mol.L-1磷酸盐缓冲液中,利用所研制的ECL传感器测定C2O24-,线性响应范围为3.0×10-7~1.0×0-5mol.L-1,检测限为1.2×10-7mol.L-1(S/N=3)。根据同样的原理也可以用来测定其他的成分,如氨基酸,TprA(三丙胺),NAD(烟酰胺腺嘌呤二核苷酸)等物质。同时应该指出的是如果使用丝网印刷机器进行印刷的话,电极的重现性和稳定性还可以进一步提高。 相似文献