Laser Micro-beam Manipulation System for Cells

1　Ｉｎｔｒｏｄｕｃｔｉｏｎ　　Ｉｎｔｈｅｇｅｎｅｅｎｇｉｎｅｅｒｉｎｇ ,ｔｈｅｂａｓｉｃｇｅｎｅｉｎｓｔｒｕｍｅｎｔｓｕｓｅｄｉｎｔｈｅｗｏｒｌｄａｔｐｒｅｓｅｎｔａｒｅ“ｍｉｃｒｏｓｃｏｐｅｍａｎｉｐｕｌａｔｉｏｎｉｎｓｔｒｕｍｅｎｔｓ” .Ｔｏｔｈｉｓｋｉｎｄｏｆｉｎｓｔｒｕｍｅｎｔｓ,Ｔｈｅｒａｔｉｏｏｆｍａｎｉｐｕｌａｔｉｏｎｓｕｃｃｅｓｓｉｓ ｇｒｅａｔｌｙａｆｆｅｃｔｅｄｂｙｆａｃｔｏｒｓｆｒｏｍｔｈｅｏｐｅｒａｔｏｒｓｂｅｃａｕｓｅｏｆｍａｎｕａｌｍａｎｉｐｕｌａｔｉｏｎａｎｄｃｏｎ…     

Collision of Bare Ion Si~(14 ) with the Same Z Slow-atom Si

1　ＩＮＴＲＯＤＵＣＴＩＯＮＩｎｔｈｉｓｐａｐｅｒｗｅｄｉｓｃｕｓｓｔｈｅｃｏｌｌｉｓｉｏｎｏｆｂａｒｅｉｏｎＳｉ14 ｗｉｔｈｔｈｅｓａｍｅＺｓｌｏｗａｔｏｍＳｉ.Ｉｎｒｅｃｅｎｔｐｕｂｌｉｃａｔｉｏｎｂａｒｅｈｉｇｈ-Ｚｉｏｎｓｃｏｌｌｉｓｉｏｎｗｉｔ...     

红外扫描成像系统中Narcissus等效温差的修正计算

根据辐射学原理，并考虑到实际探测器的接收尺寸，对现有的关于Ｎａｒｃｉｓｓｕｓ等效温差的计算进行了修正。可以在红外光学系统设计阶段评价其Ｎａｒｃｉｓｓｕｓ效应的影响程度，并为红外热成像系统提供评价Ｎａｒｃｉｓｓｕｓ效应的定量判据     

The Properties of Light Pressure Force with High Order in Laser Fields

1　Ｉｎｔｒｏｄｕｃｔｉｏｎ　　Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ｂｏｔｈｔｈｅｔｈｅｏｒｅｔｉｃａｌａｎｄｅｘｐｅｒｉｍｅｎｔａｌｉｎｖｅｓｔｉｇａｔｉｏｎｓｏｎｌａｓｅｒｃｏｏｌｉｎｇａｎｄｔｒａｐｐｉｎｇｈａｖｅｂｅｃｏｍｅｏｎｅｏｆｔｈｅｍａｊｏｒｆｉｅｌｄｓｉｎａｔｏｍｉｃ,ｍｏｌｅｃｕｌａｒａｎｄｏｐｔｉｃａｌ ｐｈｙｓｉｃｓ[1～ 8] .Ｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｌａｓｅｒｃｏｏｌｉｎｇａｎｄｔｒａｐｐｉｎｇｔｅｃｈｎｏｌｏｇｙｉｓｉｍｐｏｒｔａｎｔｆｏｒｔｈｅａｐｐｌｉｃａｔｉｏｎｓｓｕ…     

Fast Analysis for the Focusing of a Laser Beam with Amplitude Modulation and Phase Fluctuation Through an Aperture Lens

1　Ｉｎｔｒｏｄｕｃｔｉｏｎ　　Ｌａｓｅｒｂｅａｍｓｗｉｔｈａｍｐｌｉｔｕｄｅｍｏｄｕｌａｔｉｏｎｓ(ＡＭｓ)ａｎｄｐｈａｓｅｆｌｕｃｔｕａｔｉｏｎｓ (ＰＦｓ) ｐｒｏｐｏｓｅｄｂｙｔｈｅＬａｗｒｅｎｃｅ ＬｉｖｅｒｍｏｒｅＮａｔｉｏｎａｌＬａｂｏｒａｔｏｒｙ (ＬＬＮＬ)ｉｓａｔｙｐｉｃａｌｐｈｙｓｉｃａｌｍｏｄｅｌｆｏｒｈｉｇｈ ｐｏｗｅｒｌａｓｅｒｓ[1] .Ｉｎｈｉｇｈ ｐｏｗｅｒｌａｓｅｒｓｙｓｔｅｍｓ,ｓｕｃｈａｓｌａｒｇｅＮｄ∶ｇｌａｓｓｌａｓｅｒｆｕｓｉｏｎｄｒｉｖｅｒｓ,ｈｉｇｈ ｐｏｗｅｒｌａ…     

A CONCEPT FOR NEXT STEP ADVANCED TOKAMAK FUSION DEVICE

1　ＩｎｔｒｏｄｕｃｔｉｏｎＷｉｔｈｔｈｅａｄｖｅｎｔｏｆｔｈｅｎｅｗｃｅｎｔｕｒｙ ,ｔｈｅｗｏｒｌｄｗｉｄｅｑｕｅｓｔｆｏｒｍａｇｎｅｔｉｃｆｕｓｉｏｎｅｎｅｒｇｙｈａｓｍａｄｅａｄｅｃｉｓｉｖｅｓｔｅｐｆｏｒｗａｒｄ .Ｓｉｎｃｅ 1 998,ｔｈｅｆｏｃｕｓｏｆｄｅｓｉｇｎｅｆｆｏｒｔｉｎｔｈｅＩｎｔｅｒｎａｔｉｏｎａｌＴｈｅｒ ｍｏｎｕｃｌｅａｒＥｘｐｅｒｉｍｅｎｔａｌＲｅａｃｔｏｒ (ＩＴＥＲ)ｐｒｏｊｅｃｔｈａｓｂｅｅｎｏｎｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆａｎｅｗｄｅｓｉｇｎｔｏｍｅｅｔｒｅｖｉｓ…     

Carbon Nitride Thin Films Deposited by Plasma Assisted Nd∶YAG Laser Ablation of Graphite in N_2 H_2 Atmosphere

1　Ｉｎｔｒｏｄｕｃｔｉｏｎ　　Ｔｈｅｓｙｎｔｈｅｓｉｓｏｆｃａｒｂｏｎｎｉｔｒｉｄｅｔｈｉｎｆｉｌｍｓｈａｓｂｅｅｎｔｈｅｓｕｂｊｅｃｔｏｆｉｎｔｅｎｓｅｓｔｕｄｙｓｉｎｃｅｔｈｅｔｈｅｏｒｅｔｉｃａｌ ｐｒｅｄｉｃａｔｉｏｎ[1,2 ] ａｎｄｅｘｐｅｒｉｍｅｎｔａｌｒｅａｌｉｚａｔｉｏｎ[3] ｏｆ β Ｃ3Ｎ4ｔｈｉｎｆｉｌｍｓ.Ｍａｎｙａｔｔｅｍｐｔｓｈａｖｅｂｅｅｎｍａｄｅｔｏｓｙｎｔｈｅｓｉｚｅｔｈｉｓｍａｔｅｒｉａｌｂｙ ｐｈｙｓｉｃａｌａｎｄｃｈｅｍｉｃａｌｍｅｔｈｏｄｓ ,ｓｕｃｈａｓｒｅａｃｔ…     

A Biblock Wavelet Zero Tree Coding for Hyperspectral Imagery Data Compression

1　ＩｎｔｒｏｄｕｃｔｉｏｎＤｉｇｉｔｉｚｅｄｉｍａｇｅｓｎｅｅｄｓｔｏｒｉｎｇ ,ｔｒａｎｓｍｉｔｔｉｎｇａｎｄｐｒｏｃｅｓｓｉｎｇ ,ａｎｄｄａｔａｃｏｍｐｒｅｓｓｉｏｎｉｓｕｓｕａｌｌｙｉｍｐｌｅｍｅｎｔｅｄ ,ｏｂｔａｉｎｉｎｇｔｈｅｂｉｔｒａｔｅａｎｄｍａｉｎｔａｉｎｉｎｇａｎａｃｃｅｐｔａｂｌｅｆｉｄｅｌｉｔｙｏｒｉｍａｇｅｑｕａｌｉｔｙ .Ｃｏｍｐｒｅｓｓｉｏｎｃａｎｂｅａｃｈｉｅｖｅｄｂｙｔｒａｎｓｆｏｒｍｉｎｇｔｈｅｄａｔａ,ｐｒｏｊｅｃｔｉｎｇｉｔｏｎａｂａｓｉｓｏｆｆｕｎｃｔｉｏｎｓ…     

Brief Introduction to HIGH ENERGY PHYSICS AND NUCLEAR PHYSICS

ＨＩＧＨＥＮＥＲＧＹＰＨＹＳＩＣＳＡＮＤＮＵＣＬＥＡＲＰＨＹＳＩＣＳｉｓａｓｃｉｅｎｃｅｐｅｒｉｏｄｉｃａｌｆｏｃｕｓｉｎｇｏｎｓｐｅｃｉａｌｉｚｅｄｆｉｅｌｄｓｗｉｔｈｉｔｓｆｉｒｓｔｉｓｓｕｅｐｕｂｌｉｓｈｅｄｉｎ 1 977.ＩｔｉｓｓｐｏｎｓｏｒｅｄｂｙｔｈｅＩｎｓｔｉｔｕｔｅｏｆＨｉｇｈＥｎｅｒ ｇｙＰｈｙｓｉｃｓａｎｄｔｈｅＩｎｓｔｉｔｕｔｅｏｆＭｏｄｅｒｎＰｈｙｓｉｃｓ,ｔｈｅＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃｅｓ.Ｉｔｉｓａｍｏｎｔｈｌｙｊｏｕｒｎａｌ,ｄｉｓｔｒｉｂｕｔｅｄｂｏ…     

Effects of Change in the Average Permittivity on the Volume Hologram

ＥｆｆｅｃｔｓｏｆＣｈａｎｇｅｉｎｔｈｅＡｖｅｒａｇｅＰｅｒｍｉｔｔｉｖｉｔｙｏｎＴｈｅＶｏｌｕｍｅＨｏｌｏｇｒａｍ￥ＣＨＥＮＸｉｙｕａｎ（ＰｈｙｓｉｃｓＳｅｃｔｉｏｎ，Ｓｅｃｔｉｏｎ，ＦｕｓｈｕｎＰｅｔｒｏｌｅｕｍＩｎｓｔｉｔｕｔｅ，Ｆｕｓｈｕｎ１...     

Photoacoustic Tomography of Biological Sample Using Phase-controlled Focus Algorithm

Photoacoustic (PA) imaging is a new imaging modality, which converts pressure signals received by a scanning detector to a local distribution of electromagnetic absorption density. In this paper an experiment result of a photoacoustic tomography to depths of ～7 mm for a real tissue is presented, using a 532-nm pulse YAG laser. The time-resolved stress detection technique was used for PA signal detection with a high temporal resolution. A phase-controlled focus algorithm was used for image reconstruction. Images of different depth profiles in tissue were obtained. The depth resolution was 30 μm and could be up to 10 μm using a wide-band tranducer.     

频谱编码深度成像实验研究

频谱编码显微镜是用衍射光栅和光谱分析装置来获得显微图像.样品上不同的位置被不同的波长照明,通过对反射光光谱进行解码来得到空间信息.搭建了一个基于超连续光源和自制光谱仪的频谱编码显微成像系统,其横向分辨率为1.72±0.13μm(编码线方向)和1.26±0.08μm(垂直于编码线方向),测得不同横向位置处的轴向分辨率有差异.对离体猪肝组织不同部位进行了成像(可见血管、肝窦内皮细胞和肝细胞);对鸡心组织以10μm深度间隔进行成像,测得不同深度处结构信息不一样.结果表明,采用该频谱编码成像的方法能够进行高分辨的深度成像.     

KBA显微镜成像系统的成像模拟及X光成像实验的研究

KBA显微镜是一种非轴对称、非共轴的掠入射成像系统。其结构复杂,调节精度要求很高,在实际成像实验操作中难以掌握其成像特性。利用光学设计软件模拟其成像,对系统的调节和成像分析提供有益的参考。利用光学设计软件ZEMAX模拟了KBA显微镜对点源的成像过程,给出了KBA显微镜成像系统的焦深约为1 mm,景深为50 mm左右。并且由模拟可知,掠入射角对成像的影响很大。对像素尺寸约10μm的探测设备,模拟得出KBA成像系统的空间分辨力上限为3μm左右。基于星光Ⅱ装置对周期为20μm的网格靶成像,获得了KBA显微镜较为清晰的X光图像。该项工作为进一步开展掠入射成像系统的研究奠定了基础。     

基于旋转扫描探头的OCT内窥成像系统设计

为了同时获取样品的表面和深度信息,研究光学相干层析的成像原理,建立了基于光学相干层析技术的内窥系统,实现了旋转扫描成像,系统的工作波长为1 310 nm,工作带宽为80 nm.理论推导及计算机仿真得到了系统信噪比与干涉仪的分光比、反射率之间的关系并分析了理论分辨率和探测深度.提出外径为5 mm的内窥镜扫描探头,聚焦距离为12 mm,数值孔径NA为0.47,折射率分布常量A=0.218 7.利用微型电机驱动直角棱镜实现扫描,旋转速度为25 rpm,旋转一周得到640个采样点.采用多层盖玻片和洋葱表皮作为样品进行实验分析,得到了盖玻片和洋葱的图像,横向分辨率和纵向分辨率分别为10 μm和15 μm.结果表明,设计的光学相干层析内窥系统能够用于旋转扫描成像,获取更多的组织信息.     

CO2 laser photoacoustic spectra and vibrational modes of heroin, morphine and narcotine

Heroin, morphine and narcotine are very large molecules having 50, 40 and 53 atoms respectively. Moderately high resolution photoacoustic (PA) spectra have been recorded in 9.6 μm and 10.6 μm regions of CO₂ laser. It is very difficult to assign the modes of vibrations for PA bands by comparison with conventional low resolution IR spectra. The ab initio quantum chemical calculations were used for determining the molecular geometries and normal mode frequencies of vibrations of these molecules for assignments of PA spectra.     

基于电动可调焦透镜的大范围快速光片显微成像

搭建了一种基于电动可调焦透镜(electrically tunable lens)的大范围快速光片荧光显微成像系统.通过引入电动可调焦透镜与一维振镜以实现成像物平面和光片位置的快速移动,再结合高速s CMOS完成快速光片荧光显微成像.另外实验中通过改善光路与提升动态成像质量,实现了大范围扫描并减少了伪像.最终对成像性能进行测试,本系统的纵向分辨率和横向分辨率分别达到约5.5μm和约0.7μm,单幅图像稳定成像的速度约为275 frames/s,成像深度可超过138μm,能满足对具有一定尺寸的生物样本进行实时清晰成像的需求.     

Development of phase-stabilized swept-source OCT for the ultrasensitive quantification of microbubbles

This paper describes the development of a novel-phase resolved system based on swept-source optical-coherence tomography (SSOCT) for the ultrasensitive imaging and monitoring of gas microbubbles in aqueous media. The developed phase-stabilized SSOCT (PhS-SSOCT) system has an axial resolution of 10 μm, a phase sensitivity of 0.03 rad, an imaging depth of up to 6 mm in air, and a scanning speed of 20 kHz for a single A line. The performance of the sensing system was evaluated in water-containing gas microbubbles with a different diameter. The obtained results demonstrate that bubbles with a diameter greater than 10 μm could be detected by both structural imaging and phase response, whereas bubbles with diameters of less than 10 μm could be detected by the phase response of the SSOCT with a high sensitivity. The accuracy for the measurement of the diameter of gas microbubbles is limited to 10 μm in structural imaging and 0.01 μm in phase-sensitive monitoring. The results from this study indicate that PhS-SSOCT could be used to detect fast-moving microbubbles in aqueous solutions and ultimately could be applied for rapid assessment in biofluids (e.g., blood) and tissues (e.g., skin) in vivo.     

镀膜型nMCP探测效率和位置分辨优化的蒙特卡罗模拟研究

中子敏感微通道板(Neutron sensitive microchannel plate,nMCP)因其具有高探测效率和位置分辨,配合先进的读出电子学可作为能量分辨中子成像探测器的优先选择。相比于基体掺杂型的nMCP,基于原子层沉积技术(Atomic Layer Deposition,ALD)的nMCP具有原材料消耗少、通道内壁具有高的二次电子发射系数等优势。首先,通过实验对掺杂natGd型nMCP的典型中子和伽马信号进行研究。然后,采用Geant4模拟和理论计算对镀膜10B₂O₃型nMCP的孔径、壁厚、倾角和镀膜厚度进行优化。计算结果表明,当nMCP的几何参数选择为镀膜厚度1 μm、孔径10 μm、壁厚1 μm以及倾角3°时,nMCP性能达到约56%的热中子探测效率和约22 μm的位置分辨。计算结果对CSNS能量选择中子成像探测器nMCP的几何参数设计具有重要意义。     

Double-illumination photoacoustic microscopy

Recent developments of optical-resolution photoacoustic microscopy (OR-PAM) have improved its spatial resolution and imaging speed. However, the penetration depth of OR-PAM is still limited to ~1 mm in tissue, owing to the strong tissue scattering. Here, we have developed double-illumination PAM (DI-PAM), which illuminates the sample from both top and bottom sides simultaneously. Through phantom and in vivo experiments, we have demonstrated for thin targets that DI-PAM has a penetration depth of ~2 mm in tissue at 532 nm and a focal zone of 260 μm, both significant improvements over traditional reflection or transmission-mode OR-PAM.     

Holographic imaging with a nanometer resolution using compact table-top EUV laser

Holographic 2D/3D imaging with nanometer resolution using short wavelength extreme ultraviolet (EUV) light is presented in this paper. Gabor’s holograms were recorded with a highly coherent table top EUV laser with different numerical apertures demonstrating ultimately a spatial resolution of 46+/−2 nm, comparable with the illumination wavelength, in 2D holographic imaging. Three dimensional images were obtained from a single high numerical aperture hologram recorded in a high resolution photoresist and numerically reconstructed at different image planes, allowing numerical optical sectioning with a lateral resolution ∼170 nm and depth resolution of 2.4 μm. The holograms were recorded in a high resolution photoresist and digitized with an atomic force microscope. To assess the spatial resolution of the numerical reconstructions of the holograms a correlation method was used. The algorithm allows for simultaneous estimation of the resolution and the feature size of the image under analysis.