反射式数字全息显微术对细胞的研究

根据全息理论和光的衍射理论，理论分析了数字全息显微术原理，并依据四步相移和最小二乘相位展开技术，研究了重构细胞相位的方法.设计了用球面光波作为参考光的反射式数字全息显微光路，通过反射式数字全息显微术的方法分析研究了新鲜洋葱表皮细胞的形貌结构.实验以新鲜洋葱表皮细胞为样本，完成了实验检测和相位重构，得到了细胞的相位和三维信息.分析表明，系统理论分辨率应达到0.8 μm.     

数字全息显微术对细胞的研究

通过数字全息显微的方法分析研究了新鲜洋葱表皮细胞的形貌结构. 根据全息理论、光的衍射理论和透镜的相位变换性质,从理论上分析了数字全息显微原理,并依据四步相移和最小二乘去包裹技术,研究了重构细胞相位的方法.分析比较了不同参考光对数字全息显微分辨率的影响,设计了用球面光波作为参考光记录数字全息显微的装置.以新鲜洋葱表皮细胞为样本完成了实验检测和相位重构,得到了细胞的相位和三维信息,并粗略估计了细胞宽度和厚度.系统分辨率达到了1.3 μm.     

基于近场声全息的圆柱壳体内部声场重构效果的影响因素

在Neumann边界条件下基于近场声全息原理(NAH)重构了两端封闭的有限长圆柱壳体内部声场,计算出的结果与基于模态叠加法构建的内部声场进行比较,证明了重构方法的正确性,然后分别讨论了激励频率和全息面选择对NAH重构声场精度的影响以及重构面上不同位置的重构效果。     

可编程菲涅耳相位透镜应用于多平面全息投影

研究了基于菲涅耳相位透镜实现多平面全息投影的方法,采用硅基液晶相位调制器建立了多平面全息投影系统.首先,利用可编程菲涅耳相位透镜代替傅里叶透镜,将计算机生成的相位全息图与菲涅耳透镜的相位结合;其次,基于时分复用和空分复用原理提出了加载菲涅耳相位透镜与相位全息图到相位空间光调制器上的两种方法;最后,讨论了在多平面全息投影中每个单一平面实现旋转物体动态360°视角显示的方法.实验结果表明:在距离硅基液晶分别为500、800、1 100和1 400mm处的四个重构平面可以获得全息投影图像;通过动态地改变菲涅耳相位透镜的焦距,可以实现多平面全息投影.     

欠采样干涉图最小二乘相位解包裹算法改进

数字全息的再现光场相位一般具有较高的空间变化频率,而目前CCD、CMOS的空间带宽积还很有限,容易产生欠采样并导致包裹相位中有大量不可靠数据点存在,给最小二乘相位解包裹带来困难.本文将剪切干涉原理引入到数字全息再现光场的重构中,通过改变再现光场相位梯度的计算方法减少欠采样的影响,对现有的最小二乘相位解包裹算法进行了改进...     

数字微镜器件在视频全息中的应用

为研究数字微镜器件在视频全息中的应用,探讨了基于数字微镜器件的全息编码方法。基于数字微镜器件的微观物理结构,讨论二维离散微镜像素结构、开态微镜列的闪耀光栅特性和脉宽调制的微镜偏转工作原理。将数字微镜器件分辨力、微镜尺寸、间隔、旋转角度、参考光入射角以及衍射条件等因素结合到计算全息编码中,提出干涉面抽样方法、相位补偿技术和无偏置二元全息编码方法,使产生的计算全息图适应数字微镜器件独特的空间光调制特性和计算视频全息的要求。实验表明,该方法调整了重构物光波的衍射方向,集中了衍射光在重构像中的分布,增加了成像清晰度和光能有效利用率,提高了全息编码效率。     

基于相移技术的显微数字全息重构细胞相位

介绍了用显微镜物镜、压电陶瓷和CCD建立的一套测量细胞相位的显微数字全息光路，基于相移技术，给出了重构相位的理论分析，并用洋葱磷片叶细胞作为测试样品，完成了测量细胞相位的实验.结果表明：该系统可以完成细胞相位重构，系统分辨率不低于1 μm.     

Stokes参量数字全息法实现相位物体成像

为实现对相位物体的无损检测和成像,克服数字同轴全息相位物体成像技术在消除零级像和孪生像的干扰时存在的系列问题,提出一种基于Stokes参量的新的数字同轴全息技术。该方法区别于传统的利用干涉光场来记录原始像项的数字全息方法,通过测量物参光合成光束的Stokes参量来分别得到这两束光的振幅和相位差,从而准确、唯一地获得原始像项;再利用数字再现即可重构物光的振幅和相位信息。实验中对弱吸收的相位样品进行了测量,得到样品清晰的振幅和相位分布。结果表明,采用该方法对相位物体进行数字全息再现,可以克服传统同轴全息图中零级像和共轭像对相位物体信息的严重干扰,对于提取相位物体的振幅和相位信息是可行和有效的。     

数字全息术测定涡旋光束拓扑电荷数

提出了一种基于数字全息技术测定涡旋光束拓扑电荷数的方法.该方法通过数字全息技术获取涡旋光束和参考光的全息图并重构出涡旋光束的波前相位,判定相位围绕相位奇点的周期性分布来测定涡旋光束的拓扑电荷数.在拓扑电荷数取值分别为整数和分数的情况下,通过对数值模拟和实验结果的比较,表明该方法能够较准确地测定出拓扑电荷数.     

用于超声悬浮场可视化的光学全息相位倍增方法研究

基于二次曝光全息干涉测量原理,采用四倍相位倍增光路对声悬浮声压场分布进行了可视化研究.拍摄了给定模式的超声悬浮场在不同超声换能器激励电流时的光学全息图并进行光学再现,由再现光波的全息干涉条纹图样得到了反映声压场分布的光波波前相位变化,并进行了对比分析.结果表明,与无相位倍增光路相比,采用四倍相位倍增光路可使同一声压场的全息干涉条纹数目大大增加,测量灵敏度明显提高,为进一步研究声悬浮场声压分布提供了一种有效方法.     

Underwater hybrid near-field acoustical holography based on the measurement of vector hydrophone array

Hybrid near-field acoustical holography(NAH) is developed for reconstructing acoustic radiation from a cylindrical source in a complex underwater environment. In hybrid NAH,we combine statistically optimized near-field acoustical holography(SONAH) and broadband acoustical holography from intensity measurements(BAHIM) to reconstruct the underwater cylindrical source field. First,the BAHIM is utilized to regenerate as much acoustic pressures on the hologram surface as necessary,and then the acoustic pressures are taken as input to the formulation implemented numerically by SONAH. The main advantages of this technology are that the complex pressure on the hologram surface can be reconstructed without reference signal,and the measurement array can be smaller than the source,thus the practicability and efficiency of this technology are greatly enhanced. Numerical examples of a cylindrical source are demonstrated. Test results show that hybrid NAH can yield a more accurate reconstruction than conventional NAH. Then,an experiment has been carried out with a vector hydrophone array. The experimental results show the advantage of hybrid NAH in the reconstruction of an acoustic field and the feasibility of using a vector hydrophone array in an underwater NAH measurement,as well as the identification and localization of noise sources.     

Effect of sensor proximity over the non-conformal hologram plane in the near-field acoustical holography based on the inverse boundary element method

Near-field acoustical holography (NAH) is a useful tool for the identification and visualization of vibro-acoustic sources. In particular, NAH can be applied to many practical sources having irregular shape if the inverse boundary element method (BEM) is employed. Once the relation between the source and the radiated field is defined in the transfer matrix modeled by the BEM, the reconstruction of acoustic parameters on the source surface can be conducted by the multiplication of the inverse transfer matrix and the field data measured over the hologram surface. The usual practical way to measure the field data radiated from an irregular shaped source is to adopt a regular hologram surface, which can be a flat, cylindrical, or spherical shape, for the measurement ease. Then, the hologram surface is not conformal to the source surface and the resulting transfer matrix becomes further ill-posed than the conformal case. To investigate the effect of sensor proximity and distance variability on the reconstruction error, simulation and measurement were conducted for an interior problem comprised of a parallelepiped rigid box with a vibrating end plate. Flat, tilted, and randomized hologram surfaces were adopted in the test. It was shown that the reconstruction error is greatly affected by the conditioning of transfer matrix which is related to the positioning of sensor. To improve the reconstruction accuracy, an investigation was conducted to find proper field points among a large number of overdetermined field points. The number of field data was reduced gradually under various reduction schemes using condition number, effective independence value, and sensor distance. It was demonstrated that the quality of reconstruction result given by the non-conformal measurement can be improved by removing some field points that contribute to the ill-conditioning of the inverse problem. A small improvement of the reconstruction accuracy was observed by reducing the field points in the overdetermined situation. However, further reduction of the field points, becoming an underdetermined situation, yielded a drastic improvement of the reconstruction accuracy.     

基于波叠加法的近场声全息空间分辨率增强方法

提出一种基于波叠加法的近场声全息空间分辨率增强方法. 该方法在波叠加法的基础上,利用全息面声压信号求得布置在全息面附近的虚源面上的简单源源强,再根据求得的简单源源强实现对全息面声压的插值,进而利用插值后的全息面声压数据进行重建. 该方法可以提高近场声全息重建图像的空间分辨率,减少测量工作量,简化测量过程. 通过仿真对影响插值结果的参数进行了分析,给出了合理的选取范围;通过仿真和实验研究验证了该方法的有效性. 关键词： 波叠加法 近场声全息 空间分辨率     

Real-time optical reconstruction of the diffused 3D object using phase information calculated by Continuous Wavelet Transform

We propose the real time optical reconstruction of a three-dimensional (3D) object from a digitally recorded hologram. Phase distribution of the recorded hologram is numerically calculated by 1-dimensional Continuous Wavelet Transform (1D-CWT) for digital reconstruction with phase only information. Also, the phase distribution of 1D-CWT transferred to the spatial light modulator (SLM) is used to obtain optically reconstructed image. It is observed that an efficient real-time analysis can be achieved, if phase of 1D-CWT is used. In the same time, optically reconstructed 3D objects obtained by only phase information are about three times brighter than bleached hologram intensity's. So the minimum power loss can be obtained. Numerical and experimental results are presented.     

Acoustic transmission analysis on cavity resonance sound in a cylindrical cavity system: application to a Korean bell

This paper presents an analytical model for acoustic transmission characteristics of a cylindrical cavity system representing the acoustic resonance conditions of a Korean bell. The cylindrical cavity system consists of an internal cavity, a gap, an auxiliary cavity, and a rigid base. Since the internal cavity is connected to the external field through a gap, determination of the acoustic transmission characteristics becomes a coupling problem between the internal cavity and external field. The acoustic field of the internal cavity is considered by expanding the solution method of the mixed boundary problem, and the external field is addressed by modifying the radiation impedance model of a finite cylinder. The analytical model is validated by comparison with both experiment and a boundary element method. Using the analytical model, the resonance conditions are determined to maximize the resonance effect. Thus, the resonance frequencies of the bell cavity system are investigated according to the gap size and auxiliary cavity depth. By adjusting gap size or auxiliary cavity depth, the cavity resonance frequency is tuned to resonate partial tones of the bell sound. Finally, the optimal combination of gap size and auxiliary cavity depth is determined.     

Numerical reconstruction of an object image using an X‐ray dynamical diffraction Fraunhofer hologram

A numerical method of reconstruction of an object image using an X‐ray dynamical diffraction Fraunhofer hologram is presented. Analytical approximation methods and numerical methods of iteration are discussed. An example of a reconstruction of an image of a cylindrical beryllium wire is considered. The results of analytical approximation and zero‐order iteration coincide with exact values of the amplitude complex transmission coefficient of the object as predicted by the resolution limit of the scheme, except near the edges of the object. Calculations of the first‐ and second‐order iterations improve the result at the edges of the object. This method can be applied for determination of the complex amplitude transmission coefficient of amplitude as well as phase objects. It can be used in X‐ray microscopy.     

Helmholtz水声换能器弹性壁液腔谐振频率研究

针对传统Helmholtz水声换能器设计中刚性壁假设的局限性,将Helmholtz腔体的弹性计入到液腔谐振频率计算中,实现低频弹性Helmholtz水声换能器液腔谐振频率精确设计.基于细长圆柱壳腔体的低频集中参数模型,导出了腔体弹性引入的附加声阻抗表达式,得到了弹性壁条件下Helmholtz水声换能器等效电路图,给出了考虑了末端修正的弹性壁Helmholtz共振腔液腔谐振频率计算公式.利用ANSYS软件建立了算例模型,仿真分析了不同材质、半径、长度时的Helmholtz共振腔液腔谐振频率.结果对比表明弹性理论值与仿真值符合得很好,相比起传统的刚性壁理论计算结果,本文的弹性壁理论得出的液腔谐振频率值有所降低,与真实情况更加接近.本文的结论可以为精确设计低频弹性Helmholtz水声换能器提供理论支持.     

Reduction of sound transmission into a circular cylindrical shell using distributed vibration absorbers and Helmholtz resonators

A modal expansion method is used to model a cylindrical enclosure excited by an external plane wave. A set of distributed vibration absorbers (DVAs) and Helmholtz resonators (HRs) are applied to the structure to control the interior acoustic levels. Using an impedance matching method, the structure, the acoustic cavity, and the noise reduction devices are fully coupled to yield an analytical formulation of the structural kinetic energy and acoustic potential energy of a treated cylindrical cavity. Lightweight DVAs and small HRs tuned to the natural frequencies of the targeted structural and acoustic modes, respectively, result in significant acoustic and structural attenuation when the devices are optimally damped. Simulations show that significant interior noise reduction can only be achieved by adding damping to both structural and acoustic modes, which are resonant in the frequency bandwidth of interest. In order to be independent of the azimuth angle of the excitation and to avoid unwanted modal interactions, the devices are distributed evenly around the cylinder in rings. This treatment can only achieve good performance if the structure and the acoustic cavity are lightly damped.     

Optimum on-axis computer-generated hologram encoded into low-resolution phase-modulation devices

A computer-generated hologram with minimum spatial complexity is proposed for encoding arbitrary complex transmittance with low-resolution phase-modulation devices. The phase modulation of the proposed hologram is determined such that on-axis reconstruction with high signal-to-noise ratio, optimum reconstruction efficiency, and the largest possible signal bandwidth can be obtained.