多参量光声成像及其在生物医学领域的应用

光声成像兼具声学成像和光学成像两者的优点, 因而成为近十年来发展最迅速的生物医学成像技术之一. 本文介绍了光声成像的特点及其相对于广泛应用的光学成像技术和声学成像技术的优点; 其次, 解释了光声成像的成像原理, 在此基础上介绍了光声断层成像和光声显微镜这两种典型的光声成像方案, 并介绍了它们的技术特点; 然后, 介绍了光声成像对生物组织的生化特性、组织力学特性、血液流速分布、温度分布参数、微结构特性等多信息参量的提取能力, 及其在生物系统的结构成像、功能成像、代谢成像、分子成像、基因成像等多领域的应用; 最后, 展望了光声成像在生物医学领域的应用潜力并讨论了其局限性.     

声流现象的研究及其应用

本文从声流产生的机理出发，综合了影响声流的诸因素，介绍了声流现象的检测方法及声流效应的应用。     

基于光声成像的生物组织微结构定征研究进展*

光声成像是一种新兴的复合型生物医学成像技术,它既具有光学成像丰富的光学对比度,又具有声学成像成像深度深、深层组织空间分辨率高的优点。作为一种非侵入式的成像技术,光声成像逐渐显现出极大的生物医学应用潜力。该文首先介绍了光声成像的物理机制,以及光声显微镜和光声计算机断层成像这两种典型的光声成像技术;然后讨论了从光声射频信号中提取组织微结构的尺度、数量密度、弹性等特征参数的研究进展,并展望了其在组织定征和分类上的应用前景。     

光声信号的双谱分析方法研究

基于光声效应的光声光谱技术是光学与声学的有机结合, 可利用不同波长的入射光波, 产生不同的光声信号, 从而为组分识别、组织无损检测等提供高对比度图像, 是一种极具潜力的新型医学诊断技术.但光声光谱检测技术由于受检测方法的制约, 往往扫描时间较长, 而且信号的稳定性和图像识别的准确性也较差. 为了弥补单一光声光谱分析的局限性, 根据光声效应原理和振动理论, 提出了一种光声光谱与光声频谱相结合的双谱分析方法. 该方法通过光声频域信息的定量分析, 可以有效地提高不同组织的光声图像对比度, 从而提高光声成像的组分识别能力, 为光声频谱功能成像奠定理论基础. 实验结果显示, 光声光谱与光声频谱相结合的双谱分析方法可以较好地识别实验组织样品, 可实现高速、高分辨率的组分识别、组织探伤等, 具有广泛的应用前景和重要的临床诊断意义. 关键词： 光声成像 光声光谱分析 光声频谱分析     

热声系统的声功测量方法

声功测量对热声系统的研制开发具有重要意义。文中着重介绍了几种声功测量方法 ,阐述了各种方法的测量原理、仪器装置、应用特点及实用范例 ,对于热声系统中声功的测量具有一定参考价值。     

自建光声超声双模态成像系统的肿瘤成像分析

现有B超成像可以提供基于声阻抗差异的组织解剖结构信息,而近年来研究发现,光声成像可以提供标记组织成分的分布和功能信息。本文基于商用B超仪和脉冲激光系统建立了光声超声双模态成像系统,实现了超声组织结构成像和光声生物功能的同时成像。首先基于血红蛋白在某些波段的较强吸光性,实现了肿瘤内部组织血管灌注图像;其次用链接有靶向抗体的纳米颗粒作为靶向光声造影剂,对恶性肿瘤边缘和内部的血管以及血管附近的肿瘤组织进行了成像。最终,通过超声和光声的融合图像提供的肿瘤结构信息与光声图像提供的肿瘤功能信息,可以准确识别肿瘤组织。     

光声信号的声透镜层析成像研究

提出了一种用声透镜实现光声层析成像的新模式。从理论上计算出了声透镜的响应，测出了已知声场中标准物像面处的声场分布。考虑到圆形活塞振源的指向性，对代表物成像进行了理论修正，并与实验结果做了对比分析。研究表明，利用声透镜可以实现光声层析成像，并经图像重构得到了生物组织中异物的光声图像，横向、纵向分辨力较高。     

生物医学光声成像的研究进展

光声成像是21世纪初发展起来的新兴的生物医学成像技术,它同时具备光学成像和声学成像两者的优点,因而备受关注。本文对生物医学光声成像的发展进行了综述。首先,介绍了光声成像的特点以及相对于广泛应用的光学成像技术和声学成像技术的优点;其次,在成像原理上解释了光声成像优点的成因,并介绍了光声断层成像和光声显微镜这两种典型的光声成像技术;再次,详细介绍了多尺度的光声图像分辨率和成像深度,以及多信息维度的光声成像参数;最后,展望了光声成像在生物医学领域的应用潜力并讨论了其局限性。     

大张角共焦换能器对振动声成像的影响*

振动声成像是超声成像的一种重要形式,它可以得到包含共焦区组织的弹性信息和声衰减信息的信号,将接收到的信号用于成像即可获得反映组织特性的图像。该文对大张角共焦换能器作用下振动声成像中声辐射力和切变位移进行了理论计算和数值模拟,并通过改变张角变化及频率大小研究其对声辐射力和切变位移的影响。这项工作为大张角共焦换能器在振动声成像中的应用提供了理论支持。     

声致冷和声制冷机

本文介绍声致冷的基本原理、有关的数量关系和美国 ＬｏｓＡｌａｍｏｓ实验室推出的一台实验声制冷样机及其性能。     

The use of ultrasound-stimulated acoustic emission in the monitoring of modulus changes with temperature

It has been previously shown that the amplitude of the ultrasound-stimulated acoustic emission (USAE) signal is sensitive to tissue temperature and, therefore, can help detect it. Its amplitude, however, is sensitive to both acoustical and mechanical parameters, that at most frequencies have opposite effects due to temperature. In this paper, we explore the feasibility of using a frequency shift of the resonant peaks of the USAE signal for monitoring the tissue stiffness variation with temperature. In a numerical simulation, the variation of the frequency shift at different temperatures is shown. Then, in a series of experiments involving a gel phantom and porcine muscle tissue, the frequency shift variation is shown to follow the known stiffness changes due to temperature. It is also shown that this shift indicates reversible changes as well as the onset of thermal coagulative necrosis. The necrosis is marked by a monotonically increasing positive frequency shift. It was thus shown that the USAE spectrum peaks undergo a negative shift (or, downshift) when the stiffness decreases and a positive shift (or, upshift) when the stiffness increases. The experimental frequency shifted around a peak at 22.1-22.5 kHz within a range of -250 to 80 Hz and -200 to 250 Hz for the gel and muscle tissue for the temperatures of 25-70 and 30-70 degrees C, respectively. Simulation and ex vivo experimental results indicate that the USAE frequency shift method can help decouple the mechanical from the acoustical parameter dependence as well as detect the onset of thermal coagulative necrosis.     

Prospects of ultrasonically extracted food bioactives in the field of non-invasive biomedical applications – A review

Foods incorporated with bioactive compounds, called nutraceuticals, can fight or prevent or alleviate diseases. The contribution of nutraceuticals or phytochemicals to non-invasive biomedical applications is increasing. Although there are many traditional methods for extracting bioactive compounds or secondary metabolites, these processes come with many disadvantages like lower yield, longer process time, high energy consumption, more usage of solvent, yielding low active principles with low efficacy against diseases, poor quality, poor mass transfer, higher extraction temperature, etc. However, nullifying all these disadvantages of a non-thermal technology, ultrasound has played a significant role in delivering them with higher yield and improved bio-efficacy. The physical and chemical effects of acoustic cavitation are the crux of the output. This review paper primarily discusses the ultrasound-assisted extraction (USAE) of bioactives in providing non-invasive prevention and cure to diseases and bodily dysfunctions in human and animal models. The outputs of non-invasive bioactive components in terms of yield and the clinical efficacy in either in vitro or in vitro conditions are discussed in detail. The non-invasive biomedical applications of USAE bioactives providing anticancer, antioxidant, cardiovascular health, antidiabetic, and antimicrobial benefits are analyzed in-depth and appraised. This review additionally highlights the improved performance of USAE compounds against conventionally extracted compounds. In addition, an exhaustive analysis is performed on the role and application of the food bioactives in vivo and in vitro systems, mainly for promoting these efficient USAE bioactives in non-invasive biomedical applications. Also, the review explores the recovery of bioactives from the less explored food sources like cactus pear fruit, ash gourd, sweet granadilla, basil, kokum, baobab, and the food processing industrial wastes like peel, pomace, propolis, wine residues, bran, etc., which is rare in literature.     

组织光学概要

组织光学是研究光辐射能量在生物组织体内的传播规律以及有关组织光学特性的测量方法的一门新兴交叉学科．文章综述了组织光学的产生背景、发展状况、基本概念、主要理论方法和研究内容，并说明了它的应用前景．     

声图像的恢复对纹理分析参数影响的研究

本文研究了声图像的恢复对声图像纹理分析参数的影响。论文阐述了一种用于Ｂ超图像恢复的方法。它利用三个声学特性已知的超声组织仿真模块作参考,在增益与ＴＧＣ设置不变的情况下,可对均匀组织的Ｂ超图像进行恢复,论文采用了几种不同的纹理分析方法对已恢复的Ｂ超图像及未经恢复且在不同设置下采集的图像进行了分析结果的对比研究,得到了一些有实用意义的结论。     

Two-dimensional depth-resolved Mueller matrix characterization of biological tissue by optical coherence tomography

We built a polarization-sensitive optical coherence tomographic system and measured the two-dimensional depth-resolved full 4 x 4 Mueller matrix of biological tissue for what is believed to be the first time. The Mueller matrix measurements, which we made by varying the polarization states of the light source and the detector, yielded a complete characterization of the polarization property of the tissue sample. The initial experimental results indicated that this new approach reveals some tissue structures that are not perceptible in standard optical coherence tomography.     

基于δ声波场的生物组织光学断层成像研究

利用δ声波场和近红外光漫射理论实现生物组织的断层成像是一种新颖医学 成像方法. 通过构建一个δ声波场并作用到生物组织中，仅改变组织内作用点上的光学特性参数，并影响近红外光在组织中的传播特性而改变组织表面的光强分布. 利用组织表面光强改变量与作用点上组织光学参数改变量之间的内在关系，可以通过对δ声波场作用点上断层的扫描实现组织内部的断层成像. 该方法具有广泛的临床医学应用前景，为乳腺癌的早期检测提供一种有效方法. 关键词： 光学断层成像 δ声波场 漫射方程 生物组织     

Measurement of the Elasticity of Biological Soft Tissue of Finite Thickness

Elasticity is of profound significance to evaluating the function of a biological soft tissue.When the elasticity of a tissue is macroscopically changed,it means that the biological function of the tissue is abnormal and some disease or injury may occur.In the present work,an elastometer is developed to measure the elasticity of biological soft tissues.The measurement is based on the indentation method and the force is measured by the bending of the cantilever.The force-indentation data of the soft tissue is experimentally measured by this elastometer and Young's modulus of the tissue is calculated using the Hertz-Sneddon model.For comparison,a numerical model for the indentation method is established using the finite element method.The difference between the actual modulus and the measured modulus is discussed.The effect of the thickness of the specimen on the measurement is investigated.Young's moduli of beef,porcine liver and porcine kidney are experimentally measured.The results indicate that our elastometer is effective in measuring Young's modulus of a soft tissue quantitatively.     

Monte Carlo simulation of spectral reflectance using a multilayered skin tissue model

A nine-layered skin tissue model is newly developed for the Monte Carlo simulation of spectral reflectance. The derivation of the necessary parameters for each of the nine layers in the simulation is presented, in which the parameters used in the conventional three-layered model are modified on the basis of some histological findings on skin and reported examples. Using appropriate optical and geometrical parameters, simulated spectra can be produced that agree well with measured spectra. This approach provides a flexible means of spectral fitting to measured results and of estimating changes in the parameters of skin tissue.     

Energy Science,Principles, Technologies and Impacts: Second Edition,by John Andrews and Nick Jelley

It is now possible to obtain informative nuclear magnetic resonance (NMR) spectra from living tissue such as muscle. A discussion is given of the various ways in which NMR can be applied to biological material, and particular emphasis is placed on some recent phosphorus NMR studies of muscle. These studies can provide detailed information about the chemical reactions that are associated with muscular contraction. Mention is made of some of the biochemical and medical applications of NMR that may become feasible in the next few years.     

Response of a tissue equivalent proportional counter to neutrons

The absorbed dose as a function of lineal energy was measured at the CERN-EC Reference-field Facility (CERF) using a 512-channel tissue equivalent proportional counter (TEPC), and neutron dose equivalent response evaluated. Although there are some differences, the measured dose equivalent is in agreement with that measured by the 16-channel HANDI tissue equivalent counter. Comparison of TEPC measurements with those made by a silicon solid-state detector for low linear energy transfer particles produced by the same beam, is presented. The measurements show that about 4% of dose equivalent is delivered by particles heavier than protons generated in the conducting tissue equivalent plastic.