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1.
含有光敏剂的人体皮肤的激光诱导荧光光谱分析   总被引:2,自引:2,他引:0  
利用血红蛋白在578nm处有吸收峰以及在光动力疗法治疗鲜红斑痣过程中病变区血液含量减少的特性,根据测得的含有光敏剂的皮肤层激光诱导荧光光谱,计算了皮肤层光敏剂的荧光强度随时间的变化关系。通过光谱处理计算了624nm处光敏剂血卟啉(HpD)的荧光峰值强度。分析和计算结果表明,皮肤层中的血液含量的减少导致新的荧光光谱峰(578nm处)的产生,新荧光峰的产生造成624nm处荧光强度的增大,因此必须排除这种影响才能得到皮肤层由光敏剂产生的荧光强度。由光谱处理结果得到治疗过程中病变区光敏剂荧光强度的变化曲线。  相似文献   

2.
光动力学疗法新型光敏剂的光谱特性研究   总被引:25,自引:1,他引:24  
实验研究了用于光动力学诊断和治疗的新型光敏剂二磺基二邻苯二甲酰亚胺甲基酞菁锌(ZnPcS2P)癌光啉(PsD-007)、血啉甲醚(HMME)以及早期应用于临床的血卟啉衍生物(HpD)分别在生理盐水和含10%人血清生理盐水中的光谱特性。结果表明:除ZnPcS2P2的最大吸收峰位于670nm之外,其余三种光敏剂在人血清环境中的最大吸收峰都位于405nm处,但与生理盐水环境相比索瑞(Soret)峰发生了12nm的红移。在波长为413和514.5nm光源激发下,HMME,HpD和PsD-007在人血清环境中的荧光发射峰都分别位于625和690nm,但413nm光源的激发效率比514.5nm光源高出3倍左右,而且HEEM的荧光激发效率最高,HpD次之,PsD-007最低。  相似文献   

3.
血啉甲醚的时间分辨荧光光谱研究   总被引:3,自引:1,他引:2  
实验研究了用于肿瘤光动力学诊断的第二代新型光敏剂血啉甲醚(HMME)分别在不同血型人血清和生理盐水环境中的时间分辨光谱,同时还研究了在不同荧光发射峰处的荧光寿命以及光敏剂浓度对荧光寿命的影响。结果表明:HMME在625和690nm处的荧光寿命基本相同,无显著差异。HMME在生理盐水和人血清环境中的荧光寿命具有显著差异,分别为14.6和16.6ns,同时实验结果还表明光敏剂浓度对荧光寿命没有影响。结论对于开展肿瘤的时间分辨药物荧光光谱诊断与成像技术具有重要的指导意义。  相似文献   

4.
激光医学     
探讨新型光敏剂血叶琳单甲醚(HMME)激光诱发药物荧光与激光诱发自体荧光在肺癌组织中的光谱区别。15例肺癌病人(药物组)术前3h静脉注射HMME 3 rng/kg,20例肺癌病人(对照组)术前不注射HMME,使用三倍频 Nd:YAG激光(波长355 nm)和多光道分析仪(OMA)对切除肺癌标本的癌组织进行激光诱  相似文献   

5.
光动力疗法(PDT)是一种高效、安全、微创的新型治疗方法,国产光敏剂血卟啉单甲醚(HMME)介导的光动力疗法由于其成分单一、单态氧产量高及光敏周期短,临床上已用于脑胶质瘤和鲜红斑痣的治疗。为了进一步提高光动力疗效,本研究基于晶种生长法制备稳定粒径均一的吸收峰在530nm的阳离子金纳米球。采用毒性低且阴离子电解活化作用强的聚(四-苯乙烯磺酸钠)(PSS)进行修饰。利用静电吸引力将钛源TiCl_3水解的TiOH~(2+)与其结合并氧化制备AuNP@TiO_2核壳,通过改变NaHCO_3量制得不同TiO_2壳层厚度的AuNP@TiO_2核壳,并进一步将其与HMME结合制备结合体。采用紫外-可见吸收光谱、红外光谱、激光纳米粒度仪和透射电镜对所制样品进行表征。结果表明新型的光敏剂粒径均匀,结构稳定。最后采用细胞实验验证了其光动力疗效。总之,该研究合成了新型的Au@TiO_2-HMME纳米光敏剂,通过人口腔表皮样癌细胞系KB细胞与新型核壳纳米结构的光动力作用,与纯HMME相比,在最优条件下可提高约35%的细胞杀伤率。  相似文献   

6.
蒿甲醚的太赫兹时域光谱研究   总被引:2,自引:0,他引:2  
蒿甲醚是目前治疗疟疾非常有效的药物.由于其独特的药效,在临床医学中的应用比较广泛.采用太赫兹时域光谱技术测量了蒿甲醚在太赫兹波段的吸收特性,得到它在0.2~3.0 THz频率范围的特征吸收谱.应用密度泛函理论和6-31G基组计算了蒿甲醚分子的结构及其在太赫兹波段的振动频率,并据此埘实验光谱吸收峰进行了指认.理论计算表明,在1.26和2.73 THz处的吸收峰与实验上在1.24和2.73 THz处得到的吸收峰位置相对应,理论计算与实验数据吻合的较好.同时对篙甲醚的远红外振动模式进行了识别,振动模式以分子基团的骨架振动和扭转为主要特征.对蒿甲醚太赫兹光谱特性的研究结果表明太赫兹技术是一种检测中药的有效方法,这为进一步研究蒿甲醚的药效提供了理论依据.  相似文献   

7.
激光医学     
R318.51 2004064139 光动力治疗中光的有效吸收光剂量及其确定=Effective absorbed light dose in photodynamic therapy and its measurement[刊,中]/王颖(解放军总医院激光科.北京(100853)),刘凡光…∥激光生物学报.—2004,13(2).—91-96 光动力治疗中真正有效的光剂量是达到病变组织并且被组织中的光敏剂所吸收的那部分剂量,即有效吸收光剂量。明确组织中的有效吸收光剂量可以指导临床治疗,从而避免治疗剂量不足(治疗不彻底)或剂量过量(造成正常组织的热损伤)。而确定PDT中光的有效吸收剂量时,需测定组织中考察点的光辐射能流率。在目前计算和模拟组织中光辐射能流率的方法中,都需要首先确  相似文献   

8.
Wang Y  Liao XH  Gu Y  Chen R  Zeng J 《光谱学与光谱分析》2011,31(11):2969-2972
尝试利用漫反射光谱和荧光光谱检测鲜红斑痣皮肤在光动力治疗中的变化特点,用于分析治疗中组织光学特性的变化,指导光剂量的制定.在光动力治疗中,采用微型光纤光谱仪监测PWS皮肤的漫反射光谱和荧光光谱,结合PWS结构特点以及皮肤中主要吸光基团的吸收光谱,分析术中、术后相关组织成分变化及对应的光学特性变化.PDT治疗中PWS皮肤...  相似文献   

9.
应用荧光光谱技术监测了鲜红斑痣(PWS)光动力治疗(PDT)中的光敏剂血药浓度与光产物生成.以532 nm倍频Nd: YAG激光器作为PDT照射光源与荧光激发光源,以光谱仪与ICCD采集荧光光谱.在系统验证实验中,构建含有血卟啉单甲醚(HMME)的小鼠正常皮肤的荧光基本光谱,通过最小二乘光谱拟合区分HMME荧光(624 nm)与光产物荧光(652 nm).含有PSD-007的病人患区荧光光谱拟合采用相同基本光谱,获得不同病人个体具有显著差异的光敏剂血药浓度曲线与光产物生成漂白曲线.所建立的荧光光谱监测系统与光谱拟合方法可为PDT精确量化剂量学方法的建立提供技术手段,所得结果有利于制定个性化的PDT治疗方案.  相似文献   

10.
通过建立光动力治疗过程中激光、光敏剂、氧及其相互作用关系的数学模型,对组织中光动力剂量进行了模拟,研究了光动力剂量在组织内部分布变化的规律。模拟了激光剂量为50mW/cm2、100mW/cm2、150mW/cm2,光敏剂质量浓度为10mg/kg、20mg/kg、40mg/kg时光动力剂量变化。研究发现光动力剂量在组织内分布是非线性的,大小受激光强度、光敏剂剂量、氧浓度和组织光学参数等因素的影响,且光敏剂浓度的变化对光动力剂量变化的影响尤为明显。  相似文献   

11.
We investigated the effect of photodynamic therapy (PDT) with hematoporphyrin monomethyl ether (HMME) on the viability of Streptococcus mutans (S. mutans) cells on biofilms in vitro. Streptococcus mutans is the primary etiological agent of human dental caries. Since dental caries are localized infections, such plaque-related diseases would be well suited to PDT. The diode laser used in this study had the wavelength of 635 nm, whose output power was 10 mW and the energy density was 12.74 J/cm2. HMME was used as photosensitizer. Samples were prepared and divided into five groups: (1) HMME; (2) Laser; (3) HMME Laser; (4) Control group ( ) with chlorhexidine; and (5) Control group (-) with sterile physiological saline. Inoculum of S. mutans incubated with HMME also examined with fluorescence microscopy. PDT exhibited a significantly (P < 0.05) increased antimicrobial potential compared with 20 μm/mL HMME only, laser only, 0.05% chlorhexidine, and 0.9% sterile physiological saline, which reduced the S. mutans of the biofilm most effectively. Laser and 0.05% chlorhexidine were caused reduction in the viable counts of S. mutans significantly different (P < 0.05) also, but these two test treatments did not statistically differ from each other. HMME group did not statistically differ with negative control group. Fluorescence microscopy indicated that HMME localized primarily in the S. mutans of the biofilm. It was demonstrated that HMME-mediated PDT was efficient at killing S. mutans of biofilms and a useful approach in the treatment of dental plaque-related diseases.  相似文献   

12.
应用荧光光谱技术研究溶液中血卟啉单甲醚(HMME)的光漂白与光产物生成.以532 nm倍频Nd:YAG激光器照射样品,功率密度为100 mW·cm-2,以光学多通道分析仪(OMA)采集荧光光谱.照光过程与荧光光谱采集同步进行.通过构建基本光谱与最小二乘拟合,由单条实测光谱中分解求得HMME荧光(613 nm)、光产物荧光(639 nm)及自体荧光的强度.HMME初始浓度不超过10μg·mL-1时符合荧光-浓度线性函数关系.对照光过程的荧光光谱监测同时观察到HMME漂白、光产物生成与漂白,以及样品光学特性变化引起的自体荧光强度起伏.光产物漂白后的二次产物引起样品光学特性显著改变.所建立的荧光光谱探测系统与光谱分析方法可满足光敏剂漂白特性体外研究的需要,并为光动力治疗的剂量学在体监测提供有效研究方法.  相似文献   

13.
A priority line of biomedical applications of optics is the development of noninvasive diagnostic methods based on the scanning of fluorescence radiation of biosensors embedded in biological tissue. Their main advantage is a high sensitivity and selectivity with respect to given parameters of tissues and their variations. In this study, we present a method for and results of modeling of excitation and propagation of fluorescence radiation in a multilayer randomly inhomogeneous highly scattering and absorbing medium imitating human skin. The model takes into account the spatially inhomogeneous distribution of skin fluorophores and their photophysical characteristics. Both the spatial distribution of fluorescence of skin tissues and the possibility of localization of a detected fluorescence signal are studied. The spatial distribution of fluorescence centers (fluorophores) in the medium is assumed to closely follow the spatial distribution of collagen fibers of the skin. The equalization of the refractive indices at the air-skin interface is shown to lead to a higher degree of localization of the fluorescence signal detected from a biosensor located in a near-surface skin layer.  相似文献   

14.
Photodynamic therapy (PDT) is a promising method for cancer therapy. However, it is constrained by limited penetration depth of visible light, hydrophobicity of photosensitizers, and lack of tumor targeting. In this work, the photosensitizer zinc phthalocyanine (ZnPc) and upconversion nanocrystals (UCNs) are encapsulated into OQPGA‐PEG/RGD/TAT lipid micelles. The UCNs acting as a nanotransducer convert deep‐penetrating near‐infrared (NIR) light to visible light for activating the photosensitizer. OQPGA‐PEG/RGD/TAT lipid micelles are used as a carrier for the photosensitizer, with improved biocompatibility and cancer‐targeting ability. The results show that the photosensitizer ZnPc‐ and UCNs‐loaded OQPGA‐PEG/RGD/TAT lipid micelles are nanoparticles with an average size of 25 nm. The lipid micelle nanoparticles are stable in water with low leakage of photosensitizer. The absorption peak of the photosensitizer overlaps with the emission peak of UCNs, so the visible fluorescence emitted from the UCNs upon excitation by the NIR laser at 980 nm can activate the photosensitizer to produce singlet oxygen for PDT. The targeting RGD peptide and cell‐penetrating TAT peptide on the surface help the nanoparticles getting into cancer cells. The OQPGA‐PEG/RGD/TAT lipid micelles encapsulated with both the photosensitizer ZnPc and UCNs could be used for targeted PDT by using deep‐penetrating NIR light as the light source.  相似文献   

15.
Photodynamic therapy (PDT) is an approved modality for cancer treatment, which involves the administration of a photosensitive drug (PS) that is selectively accumulated in neoplastic tissues and their vasculature and subsequently can be activated with light at the appropriate wavelength to generate reactive molecular species that are toxic to tissues. In PDT, a great part of the used PS suffers degradation by light (photobleaching) that involves a decrease in the absorption and intensity of fluorescence of the photosensitizer as well as photoproduct formation evidenced by the appearance of a new absorption band. In this study, we investigated the correlation of cytotoxicity and depth of necrosis of Photogem and its photoproducts obtained previously by irradiation at 514 and 630 nm. The cytotoxicity for degraded Photogem decreases with the previous irradiation time of Photogem solution suggesting that the photoproducts of Photogem are less cytotoxics than the original formulation. A transition between the necrosed epithelium and healthy epithelium of normal liver of rats after irradiation at 630 nm was observed with irradiated and nonirradiated PS. It is observed that the depth of necrosis only at irradiation dose of 150 J/cm2 in both concentrations is greater for Photogem followed by Photogem degradated previously at 514 and then at 630 nm. The results obtained suggest that the threshold of necrosis values is lower for Photogem followed by its photoproducts formed, suggesting that the photoproducts present a low photodynamic activity. If the photosensitizer degradation happens at the same time as tumor destruction, the drug degradation can be complete before reaching the threshold of necrosis; then it is very important to control the drug concentration and light intensity of irradiation during PDT.  相似文献   

16.
To investigate the effect of photodynamic therapy (PDT) with hematoporphrin monomethyl ether (HMME) on bovine immunodeficiency virus (BIV) can provide the basis theory for photoinactivation of human immunodeficiency virus (HIV). To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-(4,5)-dimethylthiahiazol-2-yl-3,5-di-phenytetrazolium bromide (MTT) with power density of 5 and 25 mW/cm2 and energy density from 0.6 to 3 J/cm<'2>. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.  相似文献   

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