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基于麦克斯韦的电磁场理论,研究了在全内反射荧光显微术中不同偏振态的入射光所产生的 不同偏振的隐失场以及对不同取向的荧光分子荧光发射的影响.理论分析结果表明,p偏振的 入射场将产生椭圆偏振的隐失场,s偏振的入射场将产生s偏振的隐失场.随着荧光分子三维 取向的不同,这两种隐失场的激发荧光效率也将不同,由此引起荧光发射强度的各向异性分 布.据此特性,可以实现对膜表面分子三维取向的成像.
关键词:
全内反射
荧光显微术
隐失波
偏振态 相似文献
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板条固体激光器用新型棱镜腔的偏振特性 总被引:2,自引:0,他引:2
从对正三棱锥和其它偏振元件的分析出发,对板条固体激光器用两类新型棱镜腔,即共轴复式全内反射棱镜-半反/全反阴阳镜腔和复式全内反射棱镜-正三棱锥-阴阳镜折迭腔的偏振特性作了详细的理论和实验研究。 相似文献
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传统磁镊的测量精度受限于磁球的布朗涨落, 当磁力小于约10 pN时, 磁球的布朗涨落明显增大, 对应磁镊的空间分辨率显著下降. 为了提高传统磁镊在小力条件下的测量精度, 本文将全内反射荧光技术引入到磁镊技术中, 并建立相适应的“磁球-手柄-荧光微球-待测生物分子”单分子连接系统, 在小力条件下(小于10 pN)获得纳米量级的测量精度. 应用改进的磁镊对DNA发卡的折叠-去折叠态的转变过程进行了研究, 依据DNA发卡的折叠-去折叠态转变的性质对全内反射场的穿透深度进行了校正, 并结合实验结果对改进后的磁镊的测量精度进行分析. 观察了Bloom解旋酶的解旋动力学过程, 获得初步实验结果, 证实了改进的磁镊在单分子研究中的实用性.
关键词:
磁镊
全内反射荧光
DNA发卡
解旋酶 相似文献
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对室温下染料单分子进行了偶极取向和偶极再取向动力学的实验研究.利用共焦扫描显微镜光学系统与荧光偏振探测分析相结合的方法分别测量了聚合体薄膜中的单分子和无聚合体薄膜的单分子偶极方向变化特性,经采样统计测量镶嵌于聚合体薄膜中的单分子发生偶极再取向的概率约为5%—9%,无聚合体薄膜的单分子发生偶极再取向的概率约为26%.通过测量单分子荧光的偏振度轨迹曲线发现,偶极再取向存在着在多个偏振态之间的量子化跳跃行为.
关键词:
单分子
偶极取向
再取向
量子化跳跃 相似文献
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研究了全内反射在光子晶体多模波导中的作用,利用常规的耦合模理论对对称入射时的多模干涉行为和自映射现象进行了讨论和分析.研究表明,输入光场依然能沿着多模导光区高效的传输,这种结构中的光场传输是由全内反射和布喇格反射的联合作用而决定的.设计了一种全内反射偏振无关型光分束器,这种分束器在集成光学中具有重大的潜在应用价值. 相似文献
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J. C. Conboy J. L. Daschbach G. L. Richmond 《Applied Physics A: Materials Science & Processing》1994,59(6):623-629
Total internal reflection Second-Harmonic Generation (SHG) has been used to study a series of neat n-alkane/water interfaces. Polarization and incident angular-dependent measurements of the SH response show good agreement with theoretical predictions. Analysis of the incident and polarization angular-dependent SH response allows for determination of the nonlinear optical properties of molecules comprising the interfacial region. Based on Kleinman symmetry, the measured surface nonlinear susceptibilities suggest a high degree of interfacial order for octane and decane with less order indicated by the odd carbon n-alkanes examined, heptane and nonane. The SH response in reflection and transmission has been measured under a Total Internal Reflection (TIR) of the fundamental. The measured nonlinear susceptibilities in each case are found to be identical. 相似文献
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给出了不同基频光和倍频光偏振态组合情况下,淀积在固体基板上的Langmuir-Blodgett单分子层膜在面对入射光方向和背对入射光方向时,反射及透射光学二次谐波产生随入射角变化关系的理论公式。在此基础上,对一种芪盐LB膜样品进行变入射角的透射二次谐波产生研究。 相似文献
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通过对有序分子系统的线性与非线性光谱学的简化描述 ,帮助对有序分子体系光谱学进行研究的实验学家建立明确的物理图像和定量的研究工具 .这一描述是从最近对二阶非线性光学界面研究技术 ,即光学二次谐波 (SHG)和和频偏振振动光谱 (SFG VPS)的定量取向和偏振处理中推广出来的 .这一处理的方法关键在于简化线性和非线性光学中的有效极化率 ,构造出一个通用的取向泛函 ,并能通过实验参数清晰地计算出取向泛函的取向和强度因子 .同时还讨论了相干光谱技术在准确测量有序分子体系的取向和序的相比于非相干光谱方法的优点 . 相似文献
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Figliozzi P Sun L Jiang Y Matlis N Mattern B Downer MC Withrow SP White CW Mochán WL Mendoza BS 《Physical review letters》2005,94(4):047401
Optical second-harmonic generation (SHG) is used as a noninvasive probe of the interfaces of Si nanocrystals (NCs) embedded uniformly in an SiO2 matrix. Measurements of the generated SH mode verify that the second-harmonic polarization has a nonlocal dipole form proportional to (E x Delta inverted) E that depends on inhomogeneities in the incident field E, as proposed in recent models based on a locally noncentrosymmetric dipolar response averaged over the spherical NC interfaces. A two-beam SHG geometry is found to enhance this polarization greatly compared to single-beam SHG, yielding strong signals useful for scanning, spectroscopy, and real-time monitoring. This configuration provides a general strategy for enhancing the second-order nonlinear response of centrosymmetric samples, as demonstrated here for both Si nanocomposites and their glass substrates. 相似文献
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银表面光学二次谐波参数的研究 总被引:1,自引:0,他引:1
本文测量了几种玻璃-银膜界面反射二次谐波随入射光偏振角的变化,测量了超高真空中多晶银基底上冷凝银膜表面退火前后的反射二次谐波,以及超高真空中纯度为99.99%多晶银表面的反射二次谐波,计算了垂直和平行表面谐波电流参数a、b,得到对棱镜-银界面b=-0.97;α=2.1,多晶银表面α=-9,冷凝银膜退火前后α分别为7和-5.结果表明.α对表面状况极为敏感. 相似文献
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V. Torres-Zúñiga R. Castañeda-Guzmán O.G. Morales-Saavedra A.L. Pérez-Martínez T. Ogawa 《Optics and Lasers in Engineering》2011,49(12):1413-1421
The pulsed-laser photoacoustic-technique (PLPA) was implemented to characterize molecular orientational order and anisotropy in push–pull poled polymeric films as function of temperature and laser polarization. Traditionally, photoacoustic signals are considered to be directly proportional to the linear optical absorption in amorphous media. In this work, however, it is shown that photoacoustic signals can also be highly sensitive to the material anisotropy when convenient polarization dependent photoacoustic analyses are performed. Thus, variation of the molecular orientation in organic films, comprising rod-like polar chromophores, can be unambiguously monitored via rms-analyses performed on the amplitude of the generated opto-acoustical PLPA-signals as function of the incident laser polarization. This result can be useful for the characterization of organic-based nonlinear optical (NLO) poled films and, in general, in studies of anisotropic materials. In fact, in this work we were able to accurately determine the molecular order parameter (?) of a NLO-active spin-coated polymeric film containing optically active push–pull chromophores. These molecules, previously oriented via an electrical-poling procedure, are capable to exhibit strong second harmonic generation (SHG) effects. The PLPA-measurements were systematically compared to the linear UV–vis optical absorbance spectra while heating the poled film sample in order to monitor the thermally induced molecular disorder, so that the order parameter may be photo-acoustically evaluated via the PLPA-signals generated from the poled to the unpoled film phase. These PLPA-experiments were performed taking into account the UV–vis reference spectra for calibration and comparison purposes in the evaluation of the order parameter. A significant advantage of the PLPA-technique over commonly used optical spectral methodologies is its convenient applicability in samples exhibiting poor or null optical transmission. 相似文献
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Quantitative Surface Chirality Detection with Sum Frequency Generation Vibrational Spectroscopy: Twin Polarization Angle Approach 下载免费PDF全文
介绍了一种新的表面和频振动光谱方法: 定量手性检测中的双偏振角方法. 一般来讲表面光谱信号中非手性项贡献最强,而纯界面手性信号要比非手性信号弱两到三个数量级. 因此在和频振动光定量测量和分析界面手性的贡献问题上存在困难. 在和频振动光谱的双偏振角方法中,通过固定入射红外光偏振方向,并同时改变入射可见光和初涉和频信号光的偏振角进行测量. 这样所得到的偏振依赖的和频振动光谱信号能够直接给出界面手性贡献的特征,并
且能够用于准确测量界面的手性和非手性贡献. 对双偏振角方法进行了描述,并且通过S和R手性的柠檬烯空 相似文献
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We investigate one-dimensional nonlinear photonic crystals consisting of ferroelectric domains with the modulated polarization directions. Significant enhancement of second harmonic generation (SHG) is observed from numerical simulations when the frequency of fundamental wave is aimed at the photonic band edge. We devise the model structure with optimal configuration of the polarization directions of the ferroelectric domains in terms of simulated annealing algorithm. The conversion efficiencies of the ‘forward’ and ‘backward’ SHGs can be engineered. 相似文献