Resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra for studying the interaction of erythrosine with Fe(phen)3(2+) and its analytical application

In a weak alkaline Britton-Robinson buffer medium, erythrosine (Ery) can react with Fe(phen)(3)(2+) to form 1:1 ion-association complex, which will cause not only the changes of the absorption spectra, but also the remarkable enhancement of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) spectra, and the appearance of new spectra of RRS, SOS and FDS. The maximum RRS, SOS and FDS wavelengths (λ(ex)/λ(em)) of the ion-association complex are located at 358/358 nm, 290/580 nm and 780/390 nm, respectively. The increments of scattering intensities (ΔI) are directly proportional to the concentration of Ery in a certain range. The detection limits for Ery are 0.028 μg mL(-1) for RRS method, 0.068 μg mL(-1) for SOS method and 0.11 μg mL(-1) for FDS method, respectively. Among them, the RRS method has the highest sensitivity. Based on the above researches, a new highly sensitive and simple method for the determination of Ery has been developed. In this work, the spectral characteristics of absorption, RRS, SOS and FDS spectra, the optimum conditions of the reaction and influencing factors for the RRS, SOS and FDS intensities were investigated. In addition, the reaction mechanism was discussed.     

Resonance Rayleigh scattering spectra, non-linear scattering spectra of tetracaine hydrochloride-erythrosin system and its analytical application

The interaction between erythrosine (ET) and tetracaine hydrochloride (TA) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium of Britton-Robinson (BR) buffer solution of pH 4.5, erythrosine reacted with tetracaine hydrochloride to form 1:1 ion-association complex. As a result, the new spectra of RRS, SOS and FDS appeared and their intensities enhanced greatly. The maximum peaks of RRS, SOS and FDS were at 342 nm, 680 nm and 380 nm, respectively. The intensities of the three scattering were directly proportional to the concentration of TA in the range of 0.008-4.2 microg mL(-1) for RRS, 0.027-4.2 microg mL(-1) for SOS and 0.041-4.2 microg mL(-1) for FDS. The methods had very high sensitivities and good selectivities, and the detection limits were 0.003 microg mL(-1) for RRS, 0.008 microg mL(-1) for SOS and 0.012 microg mL(-1) for FDS, respectively. Therefore, a new method was developed to determinate trace amounts of TA. The recovery for the determination of TA in blood serum and urine samples was between 97.0% and 103.8%. In this study, mean polarizability was calculated by AM1 quantum chemistry method. In addition, the reasons for the enhancement of scattering spectra and the energy transfer between absorption, fluorescence and RRS were discussed.     

The interaction between furosemide-palladium (II) chelate and basic triphenylmethane dyes by resonance Rayleigh scattering spectra and resonance non-linear scattering spectra and their analytical applications

In pH 4.5–7.0 Britton-Robinson buffer solution, furosemide (FUR) reacted with Pd (II) to form a 1:1 anionic chelate. This chelate could further react with such basic triphenylmethane dyes (BTPMD) as ethyl violet (EV), crystal violet (CV), methyl violet (MV), methyl green (MeG) and brilliant green (BG) to form 1:1 ion-association complexes. This not only resulted in the change of absorption spectra, but also led to the significant enhancement of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS). The maximum RRS wavelengths were located at 324 nm for the EV, CV and MV system, and 340 nm for the BG and MeG system. The maximum SOS wavelengths were located at 550 nm for the EV, CV, BG and MeG system, and 530 nm for the MV system. The maximum scattering peaks of all the systems were at 392 nm for FDS. The enhanced RRS, SOS and FDS intensities were directly proportional to the concentration of FUR. The detection limits for the different dye systems were 0.3–4.9 ng mL^?1 for the RRS method, 3.2–33.1 ng mL^?1 for the SOS method and 9.0–85.7 ng mL^?1 for the FDS method. These methods could be used for the determination of trace amounts of FUR. The effects of the formation of ternary ion-association complexes on the spectral characteristics and intensities of absorption, RRS, SOS and FDS have been investigated. The optimum conditions of these reactions, the influencing factors and the analytical properties have been tested. The influences of coexisting substances were tested by RRS method and the results showed that this method exhibited a high sensitivity. Based on the aforementioned research, the highly sensitive, simple and rapid methods for the determination of trace amounts of FUR by resonance light scattering technique have been established, which could be applied to the determination of FUR in tablet, injection, human serum and urine samples. The composition and structure of the ternary ion-association complex and the reaction mechanism were discussed.     

Study on the interaction between torasemide and 12-tungstophosphoric acid by resonance Rayleigh scattering and resonance nonlinear scattering spectra and its analytical applications

In pH 0.6-1.1 HCl-NaAc buffer solution, torasemide (TOR) reacted with TP to form a 3:1 ion-association complexes. As a result, not only the absorption spectra were changed, but also the intensities of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) were enhanced greatly. The maximum RRS, SOS and FDS wavelengths were located at 370, 333, 776 nm, respectively. Under given conditions, the intensities of RRS, SOS and FDS were all directly proportional to the concentration of TOR. The detection limits of RRS, SOS and FDS were 0.7173 ng mL(-1), 7.007 ng mL(-1) and 10.90 ng mL(-1). The optimum conditions and the effects of coexisting substances on the reaction were investigated. The results showed that the method had good selectivity. Therefore, a highly sensitive, simple and quick method has been developed for the determination of TOR. The method can be applied satisfactorily to the determination of TOR in tablets and urine samples.     

Study on the resonance Rayleigh scattering spectra and resonance non-linear spectra of congo red-amikacin system and its analytical application

The interaction between congo red (CR) and amikacin (AMK) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium, CR combined with AMK to form an ion association complex with the composition ratio of 1∶1 by electrostatic interaction, hydrophobicity and charge transferring effect. As a result, the new spectra of RRS, FDS, and SOS appeared and their intensities were enhanced greatly. The maximum wavelengths of RRS, FDS and SOS were located at 563 nm, 475 nm and 940 nm, and the scattering intensities were proportional to the concentration of AMK. These three methods have very high sensitivities, and the detection limits were 4.0 ng·mL?1 for RRS, 3.6 ng·mL?1 for FDS and 1.9 ng·mL?1 for SOS, respectively. At the same time, the methods have better selectivity. A new method for the determination of trace amounts of AMK with congo red by resonance scattering technique has been developed. The recovery for the determination of AMK in blood serum and urine sample was between 95.5% and 105.5%. In this study, the properties, such as enthalpy of formation, charge distribution and mean polarizability, were calculated by AM1 quantum chemistry method. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed.     

十二烷基苯磺酸钠共振瑞利散射和共振非线性散射光谱法测定亚甲蓝

在0.05 mol/L（pH 1.3）的 HCl 介质中,十二烷基苯磺酸钠（SDBS）与亚甲蓝（MB）借静电引力和疏水作用力形成 2︰1 的离子缔合物,导致溶液共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)急剧增强,并产生新的 RRS,SOS 和 FDS 光谱。最大 RRS, SOS 和 FDS 分别位于 310, 647 和 341 nm, 散射强度在一定范围内与MB的浓度成正比,方法具有很高的灵敏度,对于MB的检出限(3?)分别为 1.2 ng/mL (RRS法)、1.4 ng/mL (SOS法) 和 1.7 ng/mL (FDS法)。据此发展了一种测定痕量亚甲蓝的新方法。用于人血清样品中亚甲蓝含量的检测,回收率在 94.4-103.7 ? 之间。实验优化了反应条件,考察了共存物质的影响,并结合量子化学AM1法讨论了反应机理和散射光谱产生及增强的原因。     

Resonance Rayleigh scattering, frequency doubling scattering and second-order scattering spectra of the heparin-crystal violet system and their analytical application

In pH 6.0-11.2 Britton-Robinson buffer solution, binding of heparin with crystal violet (CV) can result in a significant enhancement of resonance Rayleigh scattering (RRS) and resonance non-linear scattering, such as frequency doubling scattering (FDS) and second-order scattering (SOS). Their maximum scattering wavelengths, λ_ex/λ_em, appear at 492 nm/492 nm for RRS, 984 nm/492 nm for FDS and 492 nm/984 nm for SOS, respectively. The optimum conditions of the reaction, the influencing factors and the relationship between the three scattering intensities and the concentration of heparin have been investigated. New methods for the determination of trace amounts of heparin based on the RRS, FDS and SOS methods have been developed. The methods exhibit high sensitivities, the detection limit for heparin is 2.9 ng ml⁻¹ for the RRS method, 3.5 ng ml⁻¹ for the FDS method and 3.3 ng ml⁻¹ for the SOS method. The methods have good selectivity and were applied to the determination of heparin in heparin sodium injection samples with satisfactory results.     

Study on the resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra of the interactions of palladium(II)-ceftriaxone chelate with anionic surfactants and their analytical applications

In pH 1.8-2.9 Britton-Robinson (BR) buffer medium, ceftriaxone (CTRX) can react with palladium(II) (Pd(II)) to form 1:2 cationic chelate, which can further react with anionic surfactants (AS) such as sodium lauryl sulfonate (SLS), sodium dodecyl sulfate (SDS) and sodium dodecylbenzene sulfonate (SDBS) to form 1:3 ion-association complexes. As a result, the resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) were enhanced greatly. The maximum RRS, SOS and FDS wavelengths of three ion-association complexes were located at 335 nm, 560 nm and 390 nm, respectively. The increments of scattering intensity (DeltaI) were directly proportional to the concentrations of CTRX in certain ranges. The detection limits (3sigma) of CTRX for SLS, SDBS and SDS systems were 1.8 ng ml(-1), 2.3 ng ml(-1) and 2.3 ng ml(-1) (RRS method), 4.9 ng ml(-1), 7.4 ng ml(-1) and 4.7 ng ml(-1) (SOS method) and 6.8 ng ml(-1), 7.3 ng ml(-1) and 9.1 ng ml(-1) (FDS method), separately. The sensitivity of RRS method was higher than those of SOS and FDS methods. The optimum conditions of RRS method and the influence factors were investigated, and the composition of ion-association complexes and the reaction mechanism were discussed also. The effects of foreign substances were tested and it showed that the method has a good selectivity. Based on the ion-association reaction, the sensitive, simple and rapid methods for the determination of CTRX have been developed.     

多菌灵与Pd（II）体系的共振瑞利散射光谱及分析应用

在pH=10.0的Britton-Robinson（BR）缓冲溶液中,多菌灵与Pd(Ⅱ)反应形成1∶1的六元螯合物,导致共振瑞利散射（RRS）、二级散射（SOS）和倍频散射（FDS）显著增强,并产生新的共振瑞利散射光谱,其最大RRS、SOS和FDS波长分别位于309、606和310 nm。 在一定范围内,3种散射增强（ΔIRRS、ΔISOS和ΔIFDS）均与多菌灵的浓度成正比,反应具有较高的灵敏度,对于多菌灵的检出限分别为7.1×10-9 g/mL（RRS）、7.4×10-9 g/mL（SOS）和10.7×10-9 g/mL（FDS）。 据此提出了测定多菌灵的光散射新方法。 以灵敏度最高的RRS法为例,测定了西芹和市售农药中多菌灵的含量,结果与标准方法一致。 文中还对反应机理和散射增强的原因进行了讨论。     

呋塞米-Pd(Ⅱ)螯合物与某些碱性三苯甲烷类染料相互作用的共振瑞利散射和共振非线性散射光谱及其分析应用

在pH4.5～7.0的Britton-Robinson(BR)缓冲溶液中,呋塞米(FUR)与Pd(Ⅱ)形成1:1的螯合阴离子,它能进一步与乙基紫(EV)、结晶紫(CV)、甲基绿(MeG)、亮绿(BG)、甲基紫(MV)等碱性三苯甲烷染料(BTPMD)阳离子通过静电引力和疏水作用形成FUR:Pd(II):BTPMD为1:1:1的离子缔合物.此时,该离子缔合反应不仅能引起吸收光谱的变化,而且更能导致共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)的显著增强,其最大RRS波长分别位于324nm(EV,CV和MV体系)和340nm(BG和MeG体系),最大SOS波长分别位于550nm(EV,CV,BG和MeG体系)和530nm(MV体系),而最大FDS波长均位于392nm附近.在一定条件下三种散射增强(ΔIRRS,ΔISOS和ΔIFDS)均与呋塞米(FUR)的浓度成正比.对不同染料体系,三种方法对FUR的检出限分别在0.3～4.9ng/mL(RRS),3.2～33.1ng/mL(SOS)和9.0～85.7ng/mL(FDS)之间,均可用于痕量FUR的测定.本文研究了三元离子缔合物的形成对吸收,RRS,SOS和FDS光谱特征和强度的影响,考察了适宜的反应条件、影响因素和分析化学性质,并以RRS法为例考察了共存物质的影响.据此提出了一种高灵敏度、简便、快速测定FUR的共振光散射新方法,将其用于片剂、注射液、人血清和尿样中FUR的测定,结果满意.文中还对三元离子缔合物的组成、结构和反应机理进行了讨论.     

Ag(Ⅰ)-呋塞米相互作用的共振瑞利散射、二级散射和倍频散射光谱及其分析应用

在pH=3.5的HAc-NaAc介质中,呋塞米(FUR)与Ag(Ⅰ)形成1:1(摩尔比)的螯合物,从而引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)光谱显著增强,其最大RRS,SOS和FDS波长分别位于310,584和330 nm.在一定范围内,3种散射信号的增强(△ⅠRRs,△ⅠSOS和△ⅠFDS...     

Analytical Application of Resonance Rayleigh Scattering,Frequency Doubling Scattering,and Second-Order Scattering Spectra for the Sodium Alginate-CTAB System

Three new methods for the determination of trace amounts of sodium alginate (SA) based on the reaction of SA with cetyltrimethylammonium bromide (CTAB) by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS), and second-order scattering (SOS) have been investigated. The SA can react with CTAB in a pH 10.0 Britton–Robinson buffer to form a new product, which can lead to a significant enhancement of RRS, FDS, and SOS intensities and appearance of new spectra. The maximum scattering wavelengths, λex/λem, appear at 351 nm/351 nm for RRS, 240 nm/480 nm for SOS, and 870 nm/435 nm for FDS, respectively. The increments of the scattering intensities (ΔI) are proportional to the concentration of SA in a certain range. The detection limits (3σ) for SA are 3.69 ng mL^?1 for the RRS method, 6.91 ng mL^?1 for the FDS method, and 7.45 ng mL^?1 for the SOS method under optimum conditions. The proposed methods were applied to the determination of SA in real samples with satisfactory results.     

Resonance Rayleigh scattering spectral method for the determination of raloxifene using gold nanoparticle as a probe

When gold nanoparticles were being prepared by sodium citrate reduction method, citrate anions self-assembled on the surface of gold nanoparticles to form supermolecular complex anions with negative charges, and protonated raloxifene (Ralo) was positively charged and could bind with the complex anions to form larger aggregates through electrostatic force and hydrophobic effects, which could result in the remarkable enhancement of the resonance Rayleigh scattering intensity (RRS), and the appearance of new RRS spectra. At the same time, the second-order scattering (SOS) and frequency-doubling scattering (FDS) intensities were also enhanced. The maximum wavelengths were located near 370 nm for RRS, 520 nm for SOS, and 350 nm for FDS, respectively. Among them, the RRS method had the highest sensitivity and the detection limit was 5.60 ng mL⁻¹ for Ralo, and its linear range was 0.05-2.37 μg mL⁻¹. A new RRS method for the determination of trace Ralo using gold nanoparticles probe was developed. The optimum conditions of the reaction and influencing factors were investigated. In addition, the reaction mechanism and the reasons for the enhancement of RRS were discussed.     

Determination of Matrine and Oxymatrine in Radix Sophorae Flavescentis by Resonance Rayleigh Scattering,Second-order Scattering and Frequency Doubling Scattering Technique

In 0.1 mol/L HCl medium, 12-tungstophosphoric(TP) acid reacted with matrine(Mat) and oxymatrine(Oxy) to form an ion-association complex. As a result, the new spectra of resonance Rayleigh scattering(RRS), second-order scattering(SOS) and frequency doubling scattering(FDS) appeared and their intensities were enhanced greatly. The maximum scattering wavelengths of RRS, SOS and FDS were located at 370, 670 and 390 nm, respectively. The increments of scattering intensity were directly proportional to the concentration of Mat and Oxy in a certain range. Based on this, the method for the determination of matrine and oxymatrine has been established. It has been applied to the determination of matrine and oxymatrine in samples of Radix sophorae flavescentis with satisfactory result. The reaction mechanism and reasons of RRS enhancement were discussed.     

Study on the ternary mixed ligand complex of palladium(II)-aminophylline-fluorescein sodium by resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectrum and its analytical application

The interaction between palladium(II)-aminophylline and fluorescein sodium was investigated by resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectrum. In pH 4.4 Britton-Robinson (BR) buffer medium, aminophylline (Ami) reacted with palladium(II) to form chelate cation([Pd(Ami)]2+), which further reacted with fluorescein sodium (FS) to form ternary mixed ligand complex [Pd(Ami)(FS)2]. As a result, resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering spectrum (FDS) were enhanced. The maximum scattering wavelengths of [Pd(Ami)(FS)2] were located at 300 nm (RRS), 650 nm (SOS) and 304 nm (FDS). The scattering intensities were proportional to the Ami concentration in a certain range and the detection limits were 7.3 ng mL(-1) (RRS), 32.9 ng mL(-1) (SOS) and 79.1 ng mL(-1) (FDS), respectively. Based on it, the new simple, rapid, and sensitive scattering methods have been proposed to determine Ami in urine and serum samples. Moreover, the formation mechanism of [Pd(Ami)(FS)2] and the reasons for enhancement of RRS were fully discussed.     

[HgI4]2－-蛋白质离子缔合物体系共振瑞利散射和共振非线性散射光谱及其分析应用

在0.0035～0.0045 mol/L硫酸介质中, 牛血清白蛋白(BSA)、人血清白蛋白(HSA)、卵白蛋白(OVA)和血红蛋白(HGB)等蛋白质以带正电荷的阳离子存在. 它们能借助于静电引力和疏水作用力与配阴离子[HgI4]2－-反应形成结合产物, 此时将引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)显著增强, 并且出现新的散射光谱. 其最大RRS, SOS和FDS波长分别位于390, 760和390 nm附近. 在一定范围内, 三种散射增强(ΔIRRS, ΔISOS和ΔIFDS)与蛋白质浓度成正比, 方法具有高灵敏度, 三种方法对于不同蛋白质的检出限分别在5.7～15.9 ng/mL (RRS), 8.2～15.4 ng/mL (SOS)和11.2～22.1 ng/mL (FDS)之间, 均可用于痕量蛋白质的测定. 本文研究了[HgI4]2－与蛋白质相互作用对RRS, SOS和FDS光谱特征和强度的影响, 考察了适宜的反应条件, 并以RRS为例考察了共存物质的影响, 表明方法有良好的选择性. 据此, 利用[HgI4]2－与蛋白质的相互作用发展了一种用共振光散射技术、灵敏度高、简便、快速测定蛋白质的新方法. 本方法可用于血清和人尿中总蛋白质的测定.     

Interaction between isopoly-tungstic acid and berberine hydrochloride by resonance Rayleigh scattering spectrum.

In a weak acid medium, when an isopoly-tungstic acid reacts with berberine to form an ion-association complex, strong resonance Rayleigh scattering (RRS) appears and obvious frequency-doubling scattering (FDS) as well as second-order scattering (SOS) come into being. The scattering peaks are located at 288 and 369 nm for RRS, at 290, 370 and 390 nm for FDS, and at 480, 540 and 740 nm for SOS. Under optimum conditions, all three scattering intensities are directly proportional to the concentration of berberine and can be applied to the determination of berberine. The sensitivity of the RRS method is the highest; the detection limit (3sigma) for berberine hydrochloride is 7.6 ng/ml. The method also has good selectivity. It has been applied to the determination of berberine in pharmaceuticals and goldthread extracts with satisfactory results. In the paper, studies of the existing species of isopoly-acid and the component of the ion-association complex are reported and the reasons of enhancement of RRS are discussed.     

Resonance Rayleigh scattering spectra of Cu2+-adenine-WO4(2-) system and its analytical application

In pH 6.6-7.2 Tris-HCl buffer, Cu(2+) could react with adenine (A) to form a 1:1 coordination cation [CuA](2+), which only resulted in minor change of the absorption spectrum. However, when this cation further combined with WO(4)(2-) to form a 1:1 ternary ion-association complex [CuA]WO(4), the absorption spectrum changed a lot, and the resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) enhanced significantly. The maximum wavelengths of RRS, SOS and FDS were located at 310, 592 and 395 nm, respectively. The enhanced intensities of the three methods were proportional to the concentration of adenine in certain ranges, and the detection limit of the most sensitive RRS method was 7.4 × 10(-9) mol L(-1) (1.0 ng mL(-1)), indicating that this method could detect trace adenine. In this work, the optimum reaction conditions and the influencing factors have been studied, some potential interferences and the composition of the ion-association complex have been investigated. Meanwhile, the construction of the product and the reaction mechanism have been investigated by atomic force microscopy, transmission electron microscope and quantum chemical calculation. Accordingly, a novel RRS method for determination of adenine has been proposed and applied to detect adenine in real samples with satisfactory results.     

维生素B12与12-钨磷酸相互作用的双波长共振瑞利散射光谱及其分析应用

建立了一种双波长共振瑞利散射光谱测定维生素B12的新方法. 在pH=1.0的HCl介质中, 维生素B12(VB12)与12-钨磷酸(TP)形成摩尔比为3:1的离子缔合物, 导致双波长共振瑞利散射(DWO-RRS)、 二级散射(SOS)和倍频散射(FDS)光谱显著增强, 其最大散射波长分别位于330和370 nm(RRS), 608 nm(SOS)和386 nm(FDS). 在一定范围内, 3种散射增强(ΔIRRS, ΔISOS和ΔIFDS)均与VB12的浓度成线性关系. 该方法具有较高的灵敏度, RRS, SOS和FDS法对VB12的检出限(3σ)在2.0~7.6 ng/mL之间. 研究了反应条件和共存物质的影响, 结果表明, 该方法具有良好的选择性. 据此, 提出了简便、 快速、 准确且高灵敏测定痕量VB12的光散射新方法, 适用于片剂和尿样中VB12的测定. 还对反应机理和散射光谱增强的原因进行了讨论.     

汞(Ⅱ)-邻菲啰啉-刚果红缔合体系的共振瑞利散射和共振非线性散射光谱及分析应用

在pH=9.0的弱碱性环境中,Hg2+与邻菲啰啉(phen)反应形成无色螯合物[Hg(phen)3]2+,再与刚果红(CR)反应,形成三元离子缔合物Hg(phen)3CR,其摩尔比为1∶1。 此时引起共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)光谱显著增强,最大的散射波长分别位于578 nm(RRS法)、612 nm(SOS法)和352 nm(FDS法)。 在一定条件下,散射增强(ΔI)与Hg2+浓度呈良好的线性关系,检出限分别为2.32 μg/L(RRS法)、3.20 μg/L(SOS法)和1.56 μg/L(FDS法),考察了最佳实验条件和影响因素,表明本方法具有良好的选择性,并以RRS法为例研究了共存物质的影响。 据此建立了灵敏度高、选择性好、快速准确测定Hg2+的光散射新方法,该方法用于环境水样中Hg2+的测定取得满意结果。 并对RRS增强的原因和反应机理进行了讨论。