Surfactants as Separation Modifiers in Chemical Analyses by Thin-Layer Chromatography: A Review

JPC – Journal of Planar Chromatography – Modern TLC - The works reported in the literature (1994–2015) on the use of surfactants as separation modifiers of organic and inorganic...     

Surfactants in Thin-Layer Chromatography

Data reported in the literature on the use of surfactants as modifiers of mobile and stationary phases in thin-layer chromatography are analyzed. The features of micellar and ion-pair versions of thin-layer chromatography and the dynamic and static modifications of stationary phases with surfactants are considered.     

Analysis of PCB's by Thin-Layer Chromatography

Abstract

A screening thin layer chromatography (TLC) technique has been developed that can be done in the field. Quantitative analysis by TLC densitometry can be done in a laboratory (or field van). A densitometer is needed for quantitative work by TLC. An Apple computer may be added to use as a computational device. Semi-quantitative results may be obtained by visual comparisons. Confirmations may be made by silver halide visualization on TLC. Dehalogenation with sodium biphenyl, chlorination with antimony pentachloride, or mass spectral confirmation may be used. Quantitative analysis is possible of the derivatized positive samples.     

Triangular Thin-Layer Chromatography

Abstract

This study illustrates the use of triangular plates in thin-layer chromatographic separations. A comparison between triangular, conventional and circular thin-layer chromatography clearly indicates the superiority of triangular plates in terms of sensitivity, detection limits and savings in plates and solvent.     

Two-Phase Thin-Layer Chromatography

Abstract

Thin layer chromatography (TLC) is normally carried out on single-phase (adsorbent) plates. However, it may be necessary to use nore than one adsorbent to achieve the separation of complex mixtures. Literature reports indicate that the use of mixed phases in TLC is not very common, possibly because the plates are difficult to prepare and precoated single-phase plates of different absorbents are readily available (silica gel, reversed phase, cellulose. alumina, etc.).     

Triterpene Glycosides Analyzed by Two-Dimensional Thin-Layer Chromatography

Two-dimensional TLC with judiciously chosen solvent systems that differ in pH values is proposed for identification of triterpene glycosides in plant extracts (mono- and bisdesmosidic, acidic, sulfated, acylated)     

大豆磷脂组成的薄层色谱分析

采用国产硅胶板和二次展开的薄层色谱法，对具有重要的生物功能的大豆磷脂组成进行了分析，获得了大豆磷脂中１１个重要组分的定性结果。用已知样品量的薄层光密度扫描方法建立了卵磷脂定量方法，线性关系良好，并用于实际样品的测定，获得了满意结果。     

Anodic Sampling of Titanium by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Titanium as the pure metal or as commercial titanium alloys has been widely used in dentistry. To determine the composition of...     

蔗糖酯的薄层分析

 以硅胶G板为固定相、甲苯 乙酸乙酯 甲醇 水 (体积比为 1 0∶5∶4 5∶0 2 )为流动相 ,研究建立了蔗糖酯薄层分析方法。在蔗糖酯上行展开后 ,用脲 磷酸 水饱和正丁醇溶液显色 ,斑点呈蓝色 ,蔗糖单酯的Rf 值为 0 1 6 ,多酯的Rf 值为 0 38～ 0 93。在 70℃显色 2 0min的最佳条件下 ,蔗糖单酯的检测量为 2 5 μg～ 2 50 μg时 ,其斑点面积与其检测量有良好的线性关系。用归一法和外标法对该分析方法的准确度进行考察和认证 ,两种方法对已知单酯含量的S 1 570样品测定结果的t 检验结果分别为 |t| =0 62 7(<2 571 )和 |t|=1 1 2 3(<2 571 ) 。     

Analysis of Bromo-DragonFLY by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

薄层色谱法分离二元羧酸

利用薄层色谱法分离了二元羧酸,结果令人满意。     

Electroosmotic Circular Thin-Layer Chromatography

A new version of electroosmotic thin-layer chromatography, circular electroosmotic chromatography, was suggested and a prototype apparatus for its implementation was designed.     

Elucidation of Chemical Interactions between Crude Drugs Using Quantitative Thin-Layer Chromatography Analysis

To elucidate the interactions between crude drugs in Kampo medicines (traditional Japanese medicines), it is important to determine the content of the constituents in a cost-effective and simple manner. In this study, we quantified the constituents in crude drug extracts using thin-layer chromatography (TLC), an inexpensive and simple analytical method, to elucidate the chemical interactions between crude drugs. We focused on five crude drugs, for which quantitative high-performance liquid chromatography (HPLC) methods are stipulated in the Japanese Pharmacopoeia XVIII (JP XVIII) and compared the analytical data of HPLC and TLC, confirming that the TLC results corresponded with the HPLC data and satisfied the criteria of JP XVIII. (Z)-ligustilide, a major constituent in Japanese Angelica Root, for which a method of quantification has not been stipulated in JP XVIII, was also quantitatively analyzed using HPLC and TLC. Furthermore, Japanese Angelica Root was combined with 26 crude drugs to observe the variation in the (Z)-ligustilide content from each combination by TLC. The results revealed that combinations with Phellodendron Bark, Citrus Unshiu Peel, Scutellaria Root, Coptis Rhizome, Gardenia Fruit, and Peony Root increased the (Z)-ligustilide content. Quantifying the constituents in crude drug extracts using the inexpensive and simple TLC method can contribute to elucidating interactions between crude drugs in Kampo medicines, as proposed by the herbal-pair theory.     

Quality Control of Commercial Mustard by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Horseradish samples (Cochlearis armoracia L.) and mustard samples (Sinapis alba L.) were extracted with 70:30 (v/v)...     

酰胺反应的薄层色谱法分析

用薄层色谱法监测了二氯乙酰氯与相应的胺合成二氯乙酰胺的反应过程,方法操作简便、快速、准确。     

蛋白质的微乳液毛细管电动色谱分离研究

考察了用微乳液毛细管电动色谱(MEEKC)分离蛋白质时微乳液组成等不同因素对分离的影响,并与胶束电动色谱进行对比,探讨了其分离机理,为蛋白质的分离鉴定提供了一种有力的工具.     

Circular-Development with Overpressured Thin-Layer Chromatography

Abstract

A novel type modification of circular thin-layer chromatography has been developed, in which the layer is tightly covered by a membrane, eliminating the vapour phase over the sorbent layer. The developing solvent is pumped through the apparatus.     

International Symposium on Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Analysis of Ebastine in Pharmaceutical Preparations by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Determination of Biphenyl in Citrus Fruits by Quantitative Thin-Layer Chromatography

Abstract

Residues of the fungicide biphenyl in citrus fruits have been determined by direct scanning of spots on phosphor-impregnated high performance silica gel TLC plates under UV light. Biphenyl was separated from fruit tissue by steam liquid-liquid extraction. Recoveries from spiked samples ranged from 92–99% at 100, 50, and 10 ppm levels. The precision of the TLC determination and overall procedure are shown to be adequate for residue analysis.