Protein‐Templated Peptide Ligation

Molecular templates bind particular reactants, thereby increasing their effective concentrations and accelerating the corresponding reaction. This concept has been successfully applied to a number of chemical problems with a strong focus on nucleic acid templated reactions. We present the first protein‐templated reaction that allows N‐terminal linkage of two peptides. In the presence of a protein template, ligation reactions were accelerated by more than three orders of magnitude. The templated reaction is highly selective and proved its robustness in a protein‐labeling reaction that was performed in crude cell lysate.     

Protein‐based bioadhesives and bioglues

Bioadhesives and glues are widely used as an adjunct to conventional methods employed in healing the post‐surgical injuries and restoration of normal tissue functions. Protein‐based bioadhesives have been used for a long time, and they are a more biocompatible alternative compared with synthetic adhesives. They offer advantages such as ease of application, reduction in surgery time, improved quality and strength of the seal, and effective sealing. Also, bioadhesives are being exploited in different fields like controlled and site‐specific drug delivery systems, and in tissue engineering and regeneration. There are various marketed protein‐based glues that are available in different forms. Thus, all in all, it is a patient compliant system, thereby increasing its recent popularity. This article provides insight into different types and sources of protein‐based bioadhesives, their history of use, mechanism of adhesion. and various products that have been approved by the regulatory authorities for clinical use. It also includes information regarding the products in clinical trials and potential applications.