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碳60的发现是荣获了1996年诺贝尔化学奖的重大贡献,将我们带进了又一个化学新世界。实践中碳60分了的独特构型引起了中学化学教师和学生的极大兴趣,但尚缺少其分了模型的简易制作方法。作者在教学过程中创造了一种碳60分了模型的制作方法。 相似文献
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低维ZnO纳米材料* 总被引:1,自引:0,他引:1
本文主要综述了近年来ZnO纳米材料制备领域的一些新方法,简单比较了各种方法之间的优缺点;讨论了制备不同形貌的ZnO纳米材料的影响因素,其中重点讨论了表面活性剂的作用;介绍了ZnO纳米材料的性质及其可能的应用领域;并对ZnO纳米材料的发展前景进行了展望。 相似文献
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针对传统日用化学品化学课程教学中存在的各种问题, 进行了调整教学内容、改进教学方法和改革考核方式等方面的改革。调动了学生的学习积极性, 促进了师生间的互动, 加强了学生对知识的掌握, 提高了学生应用知识的能力, 培养了学生的创新思维。 相似文献
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针对无机化学传统教学模式的不足,构建了无机化学微信平台。介绍了构建平台必需的微信公众号和微信群的建立过程和方法。分析了无机化学微信平台在教学中的实践效果,弥补了传统教学中的不足。讨论了无机化学微信平台的积极作用,提高了教师教学水平和学生的学习能力,达到了师生双赢的目的。 相似文献
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Quantitative determination of dopamine in single rat pheochromocytoma cells by microchip electrophoresis with only one high‐voltage power supply 下载免费PDF全文
We developed a method for the direct identification of dopamine in single cultured rat pheochromocytoma cells by capillary electrophoresis using an end‐channel carbon fiber nanoelectrode amperometric detector. The operation mode was designed to achieve single‐cell injection and lysis in microfluidic chip electrophoresis with only one high‐voltage power supply. The separation and detection conditions were optimized. Four catecholamines were baseline‐separated and determined with this system, and the cell density and liquid height of the reservoirs were accommodated for single cell loading, docking and analysis. The microchip capillary electrophoresis system was successfully applied to determine dopamine in single cultured rat pheochromocytoma cells. 相似文献
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本文从细胞悬浮液制备、单个细胞进样技术、细胞溶解技术、分离模式、检测方法和应用新进展6个方面对单细胞毛细管电泳分析进行了全面评述.重点介绍了单个细胞进样技术及检测方法的最新进展,并对单细胞毛细管电泳分析的未来发展方向进行了展望. 相似文献
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微柱分离用于单个细胞分析 总被引:9,自引:1,他引:8
对90年代发展的微柱分离用于单个细胞水平的分析化学进行了评述. 系统地论述了单个细胞取样方法学,毛细管电泳及微柱液相色谱分离、电化学、激光诱导荧光、免疫、放射性检测方法及其应用于单个细胞分析的新进展. 相似文献
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Qian Feng WENG Fang Quan XIA Wen Rui JIN* School of Chemistry Chemical Engineering Shandong University Jinan 《中国化学快报》2002,13(10)
Histamine is one of the chemical mediators in connection with allergies. Only Pihel et al.1 reported a method for detection of histamine in isolated mast cells by high- performance liquid chromatography with electrochemical detection. In this method, single cells were removed and transferred to 300-nL microvials. A part of the supernatant from individual vials was injected into the chromatography column. The analysis of histamine in individual rat peritoneal mast cells using CZE with amp… 相似文献
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Electrically addressable cell traps were integrated with capillary electrophoresis for the analysis of the contents of single adherent cells. Electrodes composed of indium tin oxide were patterned on a glass surface followed by formation of topographical cell traps using 1002F photoresist. Single cells trapped in the holes could be lysed in less than 66 ms by applying a brief electric field (10 ms) across the electrode beneath the cell and the ground electrode placed in the aqueous media above the cell traps. The gas formed during cell lysis remained localized within the cavity formed by the 1002F photoresist. The retention of the gas in the cell trap enabled the cell traps to be coupled to an overlying capillary without blockage of the capillary. Single cells cultured in the traps were loaded with fluorescein and Oregon Green and then electrically lysed. By simultaneous application of an electric field to the capillary, the cell's contents were loaded into the capillary and electrophoretically separated. Orgeon Green and fluorescein from a single cell were fully resolved in less than two minutes. The use of a single patterned electrode beneath the 1002F cell trap yielded a simple easily fabricated design that was robust when immersed in aqueous solutions. Moreover, the design can easily be scaled up to create arrays of adherent cells for serial analyses using a single capillary or for parallel analysis by mating to an array of capillaries. Enhancing the rate of analysis of single adherent cells would enable a greater understanding of cellular physiology. 相似文献
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HuaZHANG WenRuiJIN 《中国化学快报》2003,14(9):952-954
A novel approach for analysis of amino acids in individual erythrocytes was established.In this method, the derivatization reagent was in~oduced into the living cells by electroporation.After derivatization, the amino acids in a single cell were determined by capillary electrophoresis with laser-induced fluorescence detection. 相似文献
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Capillary electrophoresis has been utilized for the rapid analysis of bacteria under specific experimental conditions. In this work, a method of large-volume sample stacking with polarity switching was evaluated for the analysis of bacteria by capillary electrophoresis with laser-induced fluorescence detection in order to enhance the detection sensitivity. The results indicated that the proposed method is not only effective for the focusing of bacterial cells, but also for the separation of mixtures of bacteria. With the optimized conditions, an enhancement factor of around 60-fold was obtained when long sample plug (up to 39.6% of capillary volume) was injected. Moreover, with the help of such stacking method, single, sharp, intense peak with high efficiency was observed without multiple peaks attributable to irregular clusters and aggregates of bacterial cells. This simple stacking approach appears to be promising as a rapid sterility test in various fields of applications. 相似文献