共查询到20条相似文献,搜索用时 625 毫秒
1.
James B Whitney Saied Mirshahidi So-Yon Lim Lauren Goins Chris C Ibegbu Daniel C Anderson Richard B Raybourne Fred R Frankel Judy Lieberman Ruth M Ruprecht 《Journal of immune based therapies and vaccines》2011,9(1):2
Background
We have evaluated an attenuated Listeria monocytogenes (Lm) candidate vaccine vector in nonhuman primates using a delivery regimen relying solely on oral vaccination. We sought to determine the impact of prior Lm vector exposure on the development of new immune responses against HIV antigens. 相似文献2.
Rajpal S Kashyap Aliabbas A Husain Shweta H Morey Milind S Panchbhai Poonam S Deshpande Hemant J Purohit Girdhar M Taori Hatim F Daginawala 《Journal of immune based therapies and vaccines》2010,8(1):3
Background
Tuberculosis (TB) is one of the most prevalent cause of death due to a single pathogen. Bacillus Calmette Guérin (BCG) is the only vaccine available for clinical use that protects against miliary TB; however, this vaccine has shown variable levels of efficacy against pulmonary TB. In India, a single dose of BCG vaccine is given and there are few countries where repeated doses of BCG are given. The incidence of TB in India is very high inspite of primary vaccination in neonatal period and therefore requires consideration for repeated immunization. 相似文献3.
B Anuradha CM Santosh V Hari Sai Priya G Suman Latha KJR Murthy Valluri Vijaya Lakshmi 《Journal of immune based therapies and vaccines》2007,5(1):8-7
Background
Mycobacterium bovis BCG vaccine has displayed inconsistent efficacy in different trials conducted in various geographical regions. Nevertheless, it significantly reduces the risk of severe childhood tuberculosis and continues to be used to prevent tuberculosis in many countries. Many studies revealed that efficacy of vaccine wanes with age. Most of the studies were based on in vivo and in vitro responses to tuberculin. With the advent of newer tests such as in vitro interferon-γ assays and identification of potent immunogenic mycobacterial proteins there is a need to corroborate the observations. This study aims at ascertaining the need for a booster at a later age as indicated by in vitro release of IFN-γ while evaluating Ag85A as an antigen. 相似文献4.
Briassoulis G Karabatsou I Gogoglou V Tsorva A 《Journal of immune based therapies and vaccines》2005,3(1):1
Background
The protection, which some BCG vaccines could confer against the development of tuberculosis (TB) in childhood, might be indirectly reflected by the subsequent development of BCG immune response. The objectives of the study were to examine effectiveness and possible differences of post-vaccination reaction to a lyophilized BCG at different age groups and to evaluate its protection against TB in a decade's period. 相似文献5.
Aaron G Filler Garth T Whiteside Mark Bacon Martyn Frederickson Franklyn A Howe Miri D Rabinowitz Alan J Sokoloff Terrence W Deacon Chris Abell Raj Munglani John R Griffiths B Anthony Bell Andrew ML Lever 《BMC neuroscience》2010,11(1):1-26
Background
Targeted delivery of pharmaceutical agents into selected populations of CNS (Central Nervous System) neurons is an extremely compelling goal. Currently, systemic methods are generally used for delivery of pain medications, anti-virals for treatment of dermatomal infections, anti-spasmodics, and neuroprotectants. Systemic side effects or undesirable effects on parts of the CNS that are not involved in the pathology limit efficacy and limit clinical utility for many classes of pharmaceuticals. Axonal transport from the periphery offers a possible selective route, but there has been little progress towards design of agents that can accomplish targeted delivery via this intraneural route. To achieve this goal, we developed a tripartite molecular construction concept involving an axonal transport facilitator molecule, a polymer linker, and a large number of drug molecules conjugated to the linker, then sought to evaluate its neurobiology and pharmacological behavior.Results
We developed chemical synthesis methodologies for assembling these tripartite complexes using a variety of axonal transport facilitators including nerve growth factor, wheat germ agglutinin, and synthetic facilitators derived from phage display work. Loading of up to 100 drug molecules per complex was achieved. Conjugation methods were used that allowed the drugs to be released in active form inside the cell body after transport. Intramuscular and intradermal injection proved effective for introducing pharmacologically effective doses into selected populations of CNS neurons. Pharmacological efficacy with gabapentin in a paw withdrawal latency model revealed a ten fold increase in half life and a 300 fold decrease in necessary dose relative to systemic administration for gabapentin when the drug was delivered by axonal transport using the tripartite vehicle.Conclusion
Specific targeting of selected subpopulations of CNS neurons for drug delivery by axonal transport holds great promise. The data shown here provide a basic framework for the intraneural pharmacology of this tripartite complex. The pharmacologically efficacious drug delivery demonstrated here verify the fundamental feasibility of using axonal transport for targeted drug delivery. 相似文献6.
Rosemarie M Booze David R Wallace Janelle M Silvers Barbara J Strupp Diane M Snow Charles F Mactutus 《BMC neuroscience》2006,7(1):33-9
Background
Prenatal cocaine exposure produces attentional deficits which to persist through early childhood. Given the role of norepinephrine (NE) in attentional processes, we examined the forebrain NE systems from prenatal cocaine exposed rats. Cocaine was administered during pregnancy via the clinically relevant intravenous route of administration. Specifically, we measured α2-adrenergic receptor (α2-AR) density in adolescent (35-days-old) rats, using [3H]RX821002 (5 nM). 相似文献7.
L. Harivardhan Reddy Humberto Ferreira Catherine Dubernet Sinda Lepetre Mouelhi Didier Desmaële Bernard Rousseau Patrick Couvreur 《Journal of nanoparticle research》2008,10(5):887-891
Recently, we had discovered that the linkage of nucleoside analogues to squalene, a precursor in the sterol biosynthesis,
led to amphiphilic molecules, which self-organized in water as nanoassemblies of 100–300 nm in diameter, irrespective of the
nucleoside analogue used. Thus, it was observed that the 4-(N)-trisnorsqualenoylgemcitabine (SQdFdC), the squalenoyl prodrug of the anticancer nucleoside analogue gemcitabine, was impressively
more active than its parent compound gemcitabine, both in vitro and in vivo on experimental leukaemia. Since squalene, which
is a natural constituent of shark liver and olive oil, is known to be absorbed orally, we investigated in this short note
the absorption and tissue distribution of 3H-radiolabelled SQdFdC nanoassemblies comparatively to 3H-gemcitabine after oral administration to mice. Whereas gemcitabine was found to be rapidly absorbed (t
max = 1 h), this compound underwent a rapid clearance from the plasma. Conversely, the SQdFdC nanoassemblies displayed slower
absorption followed by the progressive tissue accumulation, and they exhibited a lower clearance rate. The accumulation of
the SQdFdC nanoassemblies in tissues such as pancreas, thymus, lung, liver and spleen (except at 1 h post-administration)
was similar to that of the gemcitabine, yet exhibited significantly greater penetration and retention into the stomach and
intestinal tissues comparatively to gemcitabine. Thus, the SQdFdC nanoassemblies could be of potential interest in the treatment
of gastrointestinal tumours by oral route. 相似文献
8.
Hyung-Wook Kim Yunyoung C Chang Mei Chen Stanley I Rapoport Jagadeesh S Rao 《BMC neuroscience》2009,10(1):123-8
Background
Chronic N-Methyl-D-aspartate (NMDA) administration to rats is reported to increase arachidonic acid signaling and upregulate neuroinflammatory markers in rat brain. These changes may damage brain cells. In this study, we determined if chronic NMDA administration (25 mg/kg i.p., 21 days) to rats would alter expression of pro- and anti-apoptotic factors in frontal cortex, compared with vehicle control. 相似文献9.
Nikolai G Kamyshev Konstantin G Iliadi Julia V Bragina Elena A Kamysheva Elena V Tokmatcheva Thomas Preat Elena V Savvateeva-Popova 《BMC neuroscience》2002,3(1):9-10
Background
Starting from Benzer's initiative, the approach of forward genetics has been widely used to isolate mutations affecting learning and memory. For this aim, mainly the odor-shock conditioning was employed. We have isolated P insertional mutations affecting memory after courtship conditioning – another form of classical conditioning in Drosophila. Here we report the behavioral characteristics of one of these mutants, which we have called nemy (no extended memory). 相似文献10.
Lindsay J Hall Simon Clare Gordon Dougan 《Journal of immune based therapies and vaccines》2010,8(1):5
Background
Intranasal immunisation is potentially a very effective route for inducing both mucosal and systemic immunity to an infectious agent. 相似文献11.
Chemosensory properties of murine nasal and cutaneous trigeminal neurons identified by viral tracing
Nils Damann Markus Rothermel Barbara G Klupp Thomas C Mettenleiter Hanns Hatt Christian H Wetzel 《BMC neuroscience》2006,7(1):46-16
Background
Somatosensation of the mammalian head is mainly mediated by the trigeminal nerve that provides innervation of diverse tissues like the face skin, the conjunctiva of the eyes, blood vessels and the mucouse membranes of the oral and nasal cavities. Trigeminal perception encompasses thermosensation, touch, and pain. Trigeminal chemosensation from the nasal epithelia mainly evokes stinging, burning, or pungent sensations. In vitro characterization of trigeminal primary sensory neurons derives largely from analysis of complete neuronal populations prepared from sensory ganglia. Thus, functional properties of primary trigeminal afferents depending on the area of innervation remain largely unclear. 相似文献12.
Anna A Herasimtschuk Samantha J Westrop Graeme J Moyle Jocelyn S Downey Nesrina Imami 《Journal of immune based therapies and vaccines》2008,6(1):7
Background
Efficacious immune-based therapy in treated chronic HIV-1 infection requires the induction of virus-specific CD4+ T cells and subsequent maturation and maintenance of specific memory CD8+ T cells. Concomitant daily administration of recombinant human growth hormone (rhGH) with highly active antiretroviral therapy (HAART) was used in chronically infected patients with lipodystrophy in an attempt to reconstitute these virus-specific T-cell responses. 相似文献13.
Yevgen Leshchenko Sergei Likhodii Wendy Yue William M Burnham Jose L Perez Velazquez 《BMC neuroscience》2006,7(1):3-3
Background
Carbenoxolone (CBX) is a widely used gap junctional blocker. Considering several reports indicating that transient gap junctional blockade could be a favourable intervention following injuries to central nervous tissue, and some current enthusiasm in studies using systemic injections of CBX, it is imperative to consider the penetration of CBX into central nervous tissue after systemic administrations. So far, only very indirect evidence suggests that CBX penetrates into the central nervous system after systemic administrations. We thus determined the amounts of CBX present in the blood and the cerebrospinal fluid of rats after intraperitoneal administration, using high performance liquid chromatography 相似文献14.
Péter Baracskay Viola Kiglics Katalin A Kékesi Gábor Juhász András Czurkó 《BMC neuroscience》2009,10(1):133
Background
The impairment of the pontine reticular formation (PRF) has recently been revealed to be histopathologically connected with focal-cortical seizure induced generalized convulsive status epilepticus. To elucidate whether the impairment of the PRF is a general phenomenon during status epilepticus, the focal-cortical 4-aminopyridine (4-AP) application was compared with other epilepsy models. The presence of "dark" neurons in the PRF was investigated by the sensitive silver method of Gallyas in rats sacrificed at 3 h after focal 4-AP crystal or systemic 4-AP, pilocarpine, or kainic acid application. The behavioral signs of the developing epileptic seizures were scored in all rats. The EEG activity was recorded in eight rats. 相似文献15.
16.
So-Yon Lim Adam Bauermeister Richard A Kjonaas Swapan K Ghosh 《Journal of immune based therapies and vaccines》2006,4(1):5-10
Background
Adjuvants are known to significantly enhance vaccine efficacy. However, commercial adjuvants often have limited use because of toxicity in humans. The objective of this study was to determine the comparative effectiveness of a diterpene alcohol, phyto l and its hydrogenated derivative PHIS-01, relative to incomplete Freund's adjuvant (IFA), a commonly used adjuvant in augmenting protective immunity in mice against E. coli and S. aureus, and in terms of inflammatory cytokines. 相似文献17.
Background
Sensitivity of the gustatory system could be modulated by a number of short-term and long-term factors such as body mass, gender, age, local and systemic diseases and pathological processes, excessive alcohol drinking, drug dependence, smoking, composition of oral fluid, state of oral hygiene, consumption of some foods among many others. A few studies have demonstrated the effects of hunger and caloric satiety on sensitivity of the gustatory system in obese humans and animals. The aim of the present study was to assess the effects of short-term caloric deprivation and satiety on recognition taste thresholds of healthy, non-smoking, non-drinking, non-obese young male subjects. The two-alternative forced-choice technique was used to measure taste threshold. 相似文献18.
Kenneth H Huang Marie-Pierre Boisvert Famane Chung Maude Loignon Don Zarowny Lise Cyr Emil Toma Nicole F Bernard 《Journal of immune based therapies and vaccines》2006,4(1):7-12
Background
Despite the benefits of highly active antitretroviral therapy (HAART) for suppressing viral replication in HIV infection, virus persists and rebounds during treatment interruption (TI). This study explored whether HAART intensification with Remune™ vaccination before TI can boost HIV-1-specific immunity, leading to improved control of viremia off HAART. 相似文献19.
Nelson F Eng Srinivas Garlapati Volker Gerdts Lorne A Babiuk George K Mutwiri 《Journal of immune based therapies and vaccines》2010,8(1):4
Background
We previously demonstrated that polyphosphazenes, particularly PCEP, enhance immune responses in mice immunized subcutaneously and intranasally. The objective of the present study was to investigate the efficacy of polyphosphazenes as adjuvants when delivered through different routes of vaccine administration. 相似文献20.
Hugo Peluffo Laia Acarin Anna Arís Pau González Antoni Villaverde Bernardo Castellano Berta González 《BMC neuroscience》2006,7(1):35