Exoglycans of Yersina pseudotuberculosis

Summary 1. From a culture medium of strain 12 ofYersinia pseudotuberculosis of subtype IB a mixture of exoglycans has been isolated which consists of acidic heteropolysaccharides bound ionically to a protein component.2. The yield and monosaccharide composition of the exoglycans produced depend on the conditions of growth and the form of the colonies.3. The exoglycans of the local strain No. 12 and of a standard strain (No. 159) ofY. pseudotuberculosis of subtype IB contain monosaccharides (galactose, glucose, mannose, xylose, arabinose, fucose, glucuronic acid, glucosamine) and differ only by the ratio of the monosaccharide residues.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Scientific Center of the Academy of Sciences of the USSR. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 315–321, May–June, 1975.     

An investigation of a lipopolysaccharide-protein complex fromYersinia pseudotuberculosis by the light-scattering method

Summary An estimate has been made of the molecular weights and characteristic viscosities of aggregates of the LPPC fromYersinia pseudotuberculosis in water, and also in aqueous solutions of sodium chloride, urea, guanidine hydrochloride, and sodium dodecyl sulfate. It has been shown that a high ionic strength of the solution and the presence of disaggregating agents lead to a considerable decrease in the molecular weight of the LPPC.Pacific Ocean Institute of Bioorganic Chemistry of the Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 51–53, January–February, 1979.     

Characteristics of the molecular-mass parameters and viscosities of solutions of the lipopolysaccharide-protein complex fromYersinia pseudotuberculosis

The lipopolysaccharide-protein complex (LPPC) fromY. pseudotuberculosis isolated by extraction with trichloroacetic acid has been investigated by the methods of gel permeation chromatography, light scattering, and viscometry. The molecular-mass distributions of aggregates of the LPPC in water, 0.03 M Tris-HCl buffer (pH 8) and 0.1 M sodium chloride have been determined. A dependence of the polydispersity and dimensions of the aggregates on the concentration of the polymer and on the ionic strength of the solvent has been shown. It has been established that in water the LPPC has a high characteristic viscosity which falls with an increase in the ionic strength of the solution.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostock. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 384–388, May–June, 1982.     

Biochemical and immunochemical study of a protein preparation from the culture liquid ofYersinia pseudotuberculosis

Using ultrafiltration methods of isolation, a culture liquid ofY. pseudotuberculosis has yielded a protein fraction with a molecular mass of not less than 80–100 kDa which possesses hemagglutinating activity and the properties of an antireceptor. It has been shown for the first time by the methods of affinity chromatography, indirect hemagglutination, and indirect IEA that this protein preparation from aY. pseudotuberculosis culture interacts specifically with the Fab₂ fragment of normal human IgG.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Division of the Russian Academy of Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 784–787, November–December, 1994.     

The nature of the globulin of the algaPhyllophora nervosa

Conclusions The globulin of the algaPhyllophora is heterogeneous and consists of a number of fractions, some of which we assign to the glycoproteins. These fractions differ in their molecular weights, N-terminal amino acids, and the amount and composition of the amino acids and carbohydrates.The main difference between the fractions ofPhyllophora globulin is the nature of the carbohydrate bond with the true protein part of the molecule.Khimiya Prirodnykh Soedinenii, Vol. 6, No. 3, pp. 351–355, 1970     

Chemical composition of the low-molecular-mass fraction of Yersinia pseudotuberculosis as a function of the temperature of cultivation

The dependence of the chemical composition of the low-molecular-mass fraction of the microbial cells ofYersinia pseudotuberculosis (str. 2781) on the temperature of cultivation (6 and 37°C) has been investigated.Scientific-Research Institute of Epidemiology and Microbiology, Siberian Branch, Russian Academy of Medical Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, Nos. 3,4, pp. 312–316, May–August, 1992.     

Investigation of the core of the lipopolysaccharides ofYersinia pseudotuberculosis

Lipopolysaccharides have been isolated from two R mutants of the pseudotuberculosis microbeYersinia tuberculosis, serovar VA. Mild acid hydrolysis followed by gel filtration on Sephadex G-25 gave the core oligosaccharides OS I and OS II. Complete acid hydrolysis showed that OS I consisted of residues of D-galactose, G-glucose, two heptoses (D-glycero-D-mannoheptose and L-glycero-D-mannoheptose), and D-glucosamine in a ratio of 1:2.5:4:1, while OS II consisted of residues of D-glucose, heptoses, and D-glucosamine in a ratio of 1:2.5:0.2. On the basis of the results of monosaccharide analysis, methylation, Smith degradation, etc., a partial structure of the core oligosaccharide of the LPS ofY. pseudotuberculosis has been put forward.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 751–755, November–December, 1985.     

Composition of the essential oils ofLibanotis transcaucasica. V

Summary The compositions of the monoterpene hydrocarbon fractions of the essential oils of the fruit of seven ecological forms ofLibanotis transcaucasica Schisch. have been studied.Khimiya Prirodnykh Soedinenii, Vol. 3, No. 4, pp. 235–237, 1967     

Terpenoids of the oleoresin of Pinus pumila

Summary 1. The composition of the acid fraction of the oleresin ofPinus pumila (Pall.) Regel. has been studied, and from it has been isolated, in addition to known compounds, the methyl ester of a new labdane acetoxy acid.2. In the hydrocarbon fraction of the oleoresin 11 monoterpenes and 26 sesquiterpenes have been found (and 17 of the latter have been identified).3. The composition of the fraction of neutral oxygen-containing diterpenoids fromP. pumila differs considerably in its diterpenoid components from the corresponding fractions of the oleoresins of botanically related species-P. sibirica andP. koraiensis. Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Academy of Sciences of the USSR. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 299–302, May–June, 1976.     

Phospholipids of the seeds of two species of Erysimum

Summary The phospholipid complexes of the seeds ofErysimum diffusum andE. sylvestris have been investigated. It has been found that the amount of phospholipids in the seeds ofE. diffusum is lower than in those ofE. sylvestris, but their qualitative compositions are similar. The fatty-acid compositions of the triglycerides and of the total phospholipids of the two plants investigated have been studied. It has been established that the total phospholipids of the species ofErysimum under consideration and individual fractions of them differ by the degree of saturation and the qualitative and quantitative composition of the acids that they contain and, consequently, the phospholipids present in greatest amount differ in their molecular compositions.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 623–627, September–October, 1977.     

Chemical composition of the rhizomes of the Rhodiola rosea by the HPLC method

The component composition of extracts of the rhizomes ofRhodiola rosea has been studied by the HPLC method; the amount of rosavin has been determined and its lability on the autofermention of the raw material has been established.All-Union Scientific-Research Institute of Medicinal Plants, Scientific-Production Combine, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 188–193, March–April, 1991.     

Triterpenoids from the leaves of the siberian species of birchBetula nana andB. exilis

The compositions of the triterpene fractions of the unsaponifiable part of the ethereal extracts of the leaves ofB. nana andB. exilis have been studied. The leaves ofB. exilis have yielded a new triterpene for which the structure of 3,20(S),25-trihyproxydammar-23-ene is proposed, and a four component mixture of epimers at C-3 and C-24 of the compound 3,20(S),24-trihyproxydammar-25-ene. The composition and ratio of the components of the mixture were determined on the basis of chemical and spectral characteristics.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 352–356, May–June, 1985.     

Synthesis of (E)-dodec-3-en-1-yl acetate — The main component of sex pheromones of Scrobipalpa ocellatella and Scrobipalpopsis solanivora

(E)-Dodec-3-en-l-yl acetate — the main component of the sex pheromones ofScrobipalpa ocellatella andScrobipalpopsis solanivora — has been obtained by a three-stage synthesis from decanal and malonic acid. The overall yield of pheromone was 33% calculated on the initial aldehyde.A. S. Sadykov Institute of Bioorganic Chemistry, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 682–683, September–October, 1988.     

Chemical and immunochemical characterization of the lipopolysaccharides of Yersinia kristensenii serovars 0:12,25; 0:12,26; and 0:25,35

A comparative and immunochemical characterization of the lipopolysaccharides of three serovars ofY. kristensenii has been performed and the ratios of the monosaccharides have been established. The results of the immunochemical investigations confirmed the serotyping of the microorganisms.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, Academy of Sciences of the USSR, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 335–338, May–June, 1988.     

Lipopolysaccharides of the pseudotuberculosis microbe Yersinia pseudotuberculosis

This review generalizes the results of structural investigations of the polysaccharides of the O-specific side chains, of the core oligosaccharides, and of the lipid A of the pseudotuberculosis microbeYersinia pseudotuberculosis which causes Far-Eastern scarlatina-like fever. The complete structures of the repeating units of the specific polysaccharides from the lipopolysaccharides of serovars I A, I B, III, IV A, V A, V B, and VI which are responsible for the O-antigenic specificity of the microorganism are given. For the majority of serovars the repeating unit is represented by a branched pentasaccharide. Exceptions are the lipopolysaccharides of serovars I A and III which have a tetrasaccharide repeating unit. The presence of an aminosugar residue at the reducing end, by which the O-specific chain is attached to the oligosaccharide of the core, is common and characteristic for the lipopolysaccharides isolated from all the serovars. The structures of the core oligosaccharide and of lipid A, which are common for all serovars ofYersinia pseudotuberculosis, are given.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Branch, USSR Academy of Sciences, Vladivostok. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 163–171, March–April, 1988.     

Polysaccharides of iridaceae V. Characterization of the neutral fraction of the water soluble polysaccharide from Juno drepanophylla

From the WSPS-I of the bulbs with roots ofJuno drepanophylla, 15 fractions have been isolated by fractionation on DEAE-cellulose. Fractions 1 and 2 together made up 53% of the total weight of the fractions. Fraction 1 proved to be homogeneous on ultracentrifugation, on passage through Sepharose-4B, and on high-pressure LC. The results of the analysis of the methylated product of fraction 1 differed little from those for the initial polysaccharide.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 415–418, July–August, 1986.     

Characteristics of the water-soluble polysaccharides of the leaves ofSilphium perfoliatum

The characteristics of the water-soluble complex of the leaves ofSilphium perfoliatum are given. The presence in them of acidic components of the type of pectic substances containing considerable amounts of compounds of protein nature has been shown. It has proved to be possible to separate the latter from the carbohydrate component by fractionation on DEAE-cellulose.M. V. Lomonosov Odessa Technological Institute of the Food Industry. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 771–774, November–December, 1979.     

Insect pheromones and their analogs. XLVII. Synthesis of 11-oxododeca-3,6-diynoic acid — The acyclic precursor of a macrolide component of pheromones of Oryzaephilus mercator and O. Surinamensis

A new approach is proposed to the synthesis of 11-oxododeca-3,6-diynoic acid — the acyclic precursor of a macrolide component of pheromones ofOryzaephilus mercator andO. surinamensis — from the readily available tetrahydropyran or allylacetone via the intermediate 5-bromo-2,2-ethylenedioxypentane.Institute of Organic Chemistry, Urals Division, Russian Academy of Sciences, Ufa. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 288–292, March–April, 1993.     

Oils of the seeds of Ziziphora pedicellata and Eremostachys molluceloides

Summary The fatty oils of the seeds of the wild speciesZiziphora pedicellata andEremostachys molluceloides (Labiatae) have been investigated. The glyceride, pigment, and hydrocarbon compositions of these oils and the fatty-acid composition of the oil ofZ. pedicellata have been determined.The oil ofE. molluceloides contains laballenic acid.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 324–328, May–June, 1975.