Cover Picture: Electrophoresis 11'2011

Issue no. 11 is a regular issue consisting of 17 contributions distributed over 6 distinct parts: Part I is on fundamentals and methodologies containing 4 research articles dealing with electrokinetic transport through nanochannels, electroosmotic flow in polygonal ducts, analysis of complex samples by mass spectrometry using a sample pretreatment by preparative CITP, and CE analysis of inorganic cations in post‐blast residue extracts; Part II has 5 contributions on various aspects of proteomics and proteins including structural analysis of the recombinant therapeutic product rFVIII, electrophoretic characterization of proteins isolated by various extraction methods from ejaculated and capacitated boar sperms, CE separations of proteins using cationic poly(amidoamine) dendrimers, hemoglobin depletion in erythrocyte cytosolic samples, and peptide fractionation by acid pH SDS‐free electrophoresis; Part III is on separations of malignant cells, and particle capture in a droplet using dielectrophoresis; Part IV has two contributions on CEC separations; Part V consists of two papers on genetic variability and amplification; Part VI is on food analysis with one review article on CE applications in food analysis and one research paper on the determination of betaines in vegetable oils by CE‐MS. Thus, issue no. 11 covers many important subjects. Featured articles include: Electrokinetic transport through nanochannels (( 10.1002/elps.201000564 )) Interdigitated comb‐like electrodes for continuous separation of malignant cells from blood using dielectrophoresis (( 10.1002/elps.201000625 )) Experimental study of dielectrophoresis and liquid dielectrophoresis mechanisms for particle capture in a droplet (( 10.1002/elps.201000548 )) Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications (( 10.1002/elps.201000541 ))     

Cover Picture: Electrophoresis 16'2011

Issue no. 16 is a regular issue with “Emphasis on Sensitivity Enhancement and Detection” consisting of 18 contributions distributed over 5 distinct parts and a Fast Track paper. The Fast Track paper is on “Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning‐based approach”. The remaining 18 papers are grouped into 5 different parts. Part I and Part II represent the emphasis of this issue which involves “Sample Extraction and Enrichment and Sensitivity Enhancement” and “Detection Approaches” based on coupling CE with EC, ECL and MS. Part I has a series of 6 research papers on multifunctional magnetic nanoparticles for the enrichment of proteins, magnetic microspheres solid phase extraction of eight illegal drugs in human urine, hollow‐fiber liquid phase microextraction of nonsteroidal anti‐inflammatory drugs in wastewater, solid phase extraction to enhance sensitivity of CE for the determination of pharmaceuticals in river water, in‐line preconcentration CZE for the analysis of haloacetic acids in water, and dispersive liquid‐liquid microextraction coupled with CE for the determination of sulfonamides. Part II has 5 papers concerned with CE coupled with EC and ECL detection for the analysis of beta‐blockers, determination of nicotine and its metabolite cotinine in urine and cigarette samples by CE coupled with ECL, CE‐ECL detection for the analysis of ibandronate in drug formulation and human urine, CE‐ESI‐MS method for carbohydrate analysis, and analysis of phospholipids using MIP‐OTC in CEC‐ESI‐MS. Part III has 3 contributions on binding interaction and affinity capillary electrophoresis involving mobility shift assay for binding of DNA with NFAT3, rapid CE‐UV binding tests of environmentally hazardous compounds with polymer‐modified magnetic nanoparticles, and quantitative evaluation of lectin‐reactive glycoforms of alpha1‐acid glycoprotein using affinity CE with fluorescence detection. Part IV is on protein analysis by gel electrophoresis and has 2 contributions while Part V has 2 research papers on rice genotyping and determination of contrast agents by MEKC in urine and serum samples. Featured articles include: FAST TRACK: Barcoding of Giardia duodenalis isolates and derived lines from an established cryobank by a mutation scanning‐based approach. (( 10.1002/elps.201100283 )) Applications of multifunctional magnetic nanoparticles for the enrichment of proteins for PAGE separation. (( 10.1002/elps.201000657 )) Dispersive liquid‐liquid microextraction coupled with capillary electrophoresis for simultaneous determination of sulfonamides with the aid of experimental design. (( 10.1002/elps.201100142 )) Carbohydrate analysis by capillary electrophoresis‐microelectrospray ionization‐mass spectrometry. (( 10.1002/elps.201100027 )) Quantitative evaluation of lectin‐reactive glycoforms of α1‐acid glycoprotein using lectin affinity capillary electrophoresis with fluorescence detection. (( 10.1002/elps.201100146 )) High throughput functional marker assay for detection of Xa/xa and fgr genes in rice (Oryza sativa L.). (( 10.1002/elps.201100196 ))     

Cover Picture: Electrophoresis 3'2012

Issue no. 3 is a regular issue consisting of 17 contributions distributed over 5 separate parts. The issue starts with 4 research articles on micro‐ and nanofluidics making up Part I. This is followed by Part II that has 5 research articles involving studies on proteins, peptides and proteomics. Part III has 3 contributions dealing with studies on nucleic acids. Part IV has 3 research articles on enantioseparation methodologies. The last part (Part V) consists of 2 contributions on various aspects of preconcentration in CE of drug of abuse and their metabolites and the sensitive monitoring for ribonucleotide reductase activity. This issue, with as few as 17 contributions, is rich in very important ideas in the field for further investigations. Featured articles include: Modeling of droplet traffic in interconnected microfluidic ladder devices (( 10.1002/elps.201100320 )) Role of streaming potential on pulsating mass flow rate control in combined electroosmotic and pressure‐driven microfluidic devices (( 10.1002/elps.201100414 )) Carotid atherosclerotic plaques: Proteomics study after a low‐abundance protein enrichment step (( 10.1002/elps.201100395 )) Quantitative experimental determination of primer‐dimer formation risk by free‐solution conjugate electrophoresis (( 10.1002/elps.201100452 )) Electromembrane extraction combined with cyclodextrin‐modified capillary electrophoresis for the quantification of trimipramine enantiomers (( 10.1002/elps.201100426 ))     

Cover Picture: Electrophoresis 4'2010

Issue no. 4 is a regular issue with Emphasis on “Bioanalysis”. Part I has 12 articles on bioanalysis featuring methodologies for proteomics, proteins, peptides and nucleic acids. Part II has 5 papers on interactive CE, e.g., MEEKC, MEKC and ACE. The remaining 2 articles make up Part III, and are concerned with the regulation of flow in microfluidics and sensitivity enhancement in CEC. Featured articles include: Identification of candidate biomarkers in ovarian cancer serum by depletion of highly abundant proteins and differential in gel electrophoresis. (( 10.1002/elps.200900441.R1 )) An effective SCAR‐PCR method derived from restriction site amplified polymorphism (RSAP) for the identification of female Schistosoma japonicum of zoonotic significance. (( 10.1002/elps.200900615.R1 )) Rapid and sensitive DNA targets detection using enzyme amplified electrochemical detection based on microchip. (( 10.1002/elps.200900538.R1 ))     

Cover Picture: Electrophoresis 14'2011

Issue no. 14 is a regular issue consisting of 17 contributions distributed over 3 distinct parts. Part I has 7 articles describing studies on proteins and proteomics including measuring protein mobility using laser Doppler electrophoresis, high sensitivity protein analysis by FESI‐CE‐MALDI‐MS, protocol for SDS‐PAGE separation of myosin heavy chain isoforms, proteomics analysis of a spring wheat cultivar in response to prolonged cold stress, analysis of changes in the 20S proteasome, treatment of acute lymphoblastic leukemia with L‐asparaginase, and improved method for immunostaining of mucin. Part II is on nucleic acids and has 4 contributions on multiplex PCR‐CE analysis, copy number variation, ethnic difference, SNPs, CE‐SSCP, allelic ladder and SNaPshot technique. Part III has 6 articles on various fundamentals and methodologies, including nanofluidics, nanopore sensing, sequential injection setup for CIEF combined with MS detection, determination of imatinib mesylate in chronic myeloid leukemia patients by CE, UV detection of plasma malondialdehyde using CE‐FASI, enantioseparation of basic drugs by CE using clarithromycin lactobionate chiral selector and CE determination of bioactive constituents in Herba Houttuyniae. Featured articles include: Advances in the measurement of protein mobility using laser Doppler electrophoresis – the diffusion barrier technique (( 10.1002/elps.201100108 )) Improved method for immunostaining of mucin separated by supported molecular matrix electrophoresis by optimizing the matrix composition and fixation procedure (( 10.1002/elps.201000608 )) Strategy for high‐fidelity multiplex DNA copy number assay system using capillary electrophoresis devices (( 10.1002/elps.201100093 )) Electrokinetic particle translocation through a nanopore containing a floating electrode (( 10.1002/elps.201100050 )) Sequential injection setup for capillary isoelectric focusing combined with MS detection (( 10.1002/elps.201100012 ))     

Cover Picture: Electrophoresis 2'2012

Issue no. 2 is a regular issue consisting of 18 contributions distributed over 5 distinct parts. The issue has an “Emphasis on Proteins and Proteomics” with 7 contributions featured in Part I. This is followed by Part II that has 4 research articles on nanopore and nanoparticles based analyses. Part III has 2 contributions on agarose gel electrophoresis for the analysis of the structure fluidity of a phage T3 DNA packaging intermediate and analyzing RNA quality. Part IV has two research articles on microchip capillary electrophoresis with poly(ethylene glycol)‐coated microfluidic devices and poly(dimethylsiloxane) microchips using a mixture of ionic and zwitterionic surfactants. The last part (Part V) consists of 3 contributions on CE of some common controlled stimulants, CE test of a blood‐brain barrier model and the simultaneous determinations of nitrite and nitrate in human plasma by CE. This issue is populated by a collection of very important research articles. It is a must read. Featured articles include: The challenge to quantify proteins with charge trains due to isoforms or conformers (( 10.1002/elps.201100321 )) Electrophoretically‐driven SDS removal and protein fractionation in the shotgun analysis of membrane proteomes (( 10.1002/elps.201100364 )) Modeling and simulation of nanoparticle separation through a solid state nanopore (( 10.1002/elps.201100201 )) A novel open‐tubular capillary electrochromatography with magnetic nanoparticle coating as stationary phase (( 10.1002/elps.201100337 )) Direct visualization of electrophoretic mobility shift assays using nanoparticle‐aptamer conjugates (( 10.1002/elps.201100308 ))     

Cover Picture: Electrophoresis 2'2011

Issue no. 2 is a regular issue assembled of 16 solid and original research articles distributed over 3 distinct parts. Part I is on novel trends in fundamentals and methodologies including theoretical models for selectivity of charged solutes in MEKC, system peaks in indirect detection, measuring epimerization constants by MEEKC, bundled CE using micro‐structured fibers, 2‐D separations by coupling CIEF and CEC, high speed DNA CE, MCE of N‐glycans and mucin expression in a microfluidic gradient device. Part II is concerned with detection, sensitivity enhancement, on‐column preconcentration and microdialysis sampling involving the design of continuous full filling CEC‐ESI‐MS using nanoparticles, CE‐fluorescence using tapered optical fiber, CZE separation of pesticide residues in water samples with acid‐assisted on‐column preconcentration and CE‐LIF to detect neurotransmitter amino acids and carbamathione in brain microdialysis samples. Novel methods for the separation and profiling of various proteins and large nucleic fragments are described in 4 consecutive papers grouped in part III. Featured articles include: Theoretical models of separation selectivity for charged compounds in micellar electrokinetic chromatography (( 10.1002/elps.201000405 )) Bundled capillary electrophoresis using microstructured fibres ( 10.1002/elps.201000442 )) Two‐dimensional separation system by on‐line hyphenation of capillary isoelectric focusing with pressurized capillary electrochromatography for peptide and protein mapping ( 10.1002/elps.201000419 )) Microchip electrophoresis of N‐glycans on serpentine separation channels with asymmetrically tapered turns ( 10.1002/elps.201000461 ))     

Cover Picture: Electrophoresis 12'2011

Issue no. 12 is a regular issue consisting of 18 contributions distributed over 4 distinct parts: Part I is on protein and proteomics methodologies containing 6 research articles dealing with new fluorescent probe for gel electrophoresis of proteins, treatment of serum samples with combinatorial library beads, glycoproteomics analysis of haptoglobin multimers, micropreparative isoelectric focusing in a suspended drop, and 2‐DE and MALDI‐TOF‐MS analysis of the therapeutic protein abatacept; Part II has 4 contributions on various approaches of genotyping; Part III treats on‐line concentration and sensitivity enhancement of angiotensins and pyridine‐triphenylborane and its degradation products; Part IV has 6 contributions on some aspects of fundamentals and methodologies involving EMMA, effects of carbon dioxide in CE at high pH, particle sorting by dielectrophoresis, CE‐electrochemiluminescence with ionic liquid, enantioseparation by monolithic imprinted capillary clamped in a chip, and infrared‐based temperature measurements in CE. In summary, issue no. 12 covers many important subjects in the field. Featured articles include: Design and synthesis of new fluorescent probe for rapid and highly sensitive detection of proteins via electrophoretic gel stain (( 10.1002/elps.201000691 )) Glycoproteomic analysis and molecular modeling of haptoglobin multimers (( 10.1002/elps.201000464 )) PCR‐free digital minisatellite tandem repeat genotyping (( 10.1002/elps.201000073 )) A dielectrophoretic continuous flow sorter using integrated microelectrodes coupled to a channel constriction (( 10.1002/elps.201000675 ))     

Cover Picture: Electrophoresis 21'2011

Issue no. 21 is a regular issue consisting of one FAST TRACK article on “Blinded study determination of high sensitivity and specificity microchip electrophoresis‐SSCP/HA to detect mutations in the p53 gene” and 19 other contributions distributed over 5 distinct parts. Part I is on proteins and proteomics containing 5 contributions describing on‐line combination of multiple chromatographic modes for multicomponent separation of proteomics and glycoproteomics, the heterogeneity of plasma‐derived clotting factor VIII, rapid species evaluation of seafood spoilage and pathogenic Gram‐positive bacteria by MALDI‐TOF mass fingerprinting, comparison of depletion and equalization strategies in proteomics, and a competent extraction method for 2‐D gel electrophoresis. Part II is on affinity capillary electrophoresis and has 2 contributions on the application of partial‐filling using lectins and glycosidases for the characterization of oligosaccharides in therapeutic antibodies, and on protonated diamines as anion‐binding agents and their utility in CE separations. 5 contributions on detection and sensitivity enhancement make up the body of Part III. DNA translocation and depurination constitute the content of the two contributions of Part IV. Finally, Part V assembles 5 papers on various fundamentals and methodologies including the influence of membrane layer properties on the electrophoretic behavior of a soft particle, evaluation of the separation mechanism of EKC with microemulsion and CDs using NMR and molecular modeling, linear polyalkylamines as fingerprinting agents in CE of low‐molecular weight heparins and glycosaminoglycans, use of CE‐SDS gel for the characterization of monoclonal antibody hinge region clipping due to copper and high pH stress, and electrophoretic isolation of saponin fractions from Saponinum album and their evaluation in synergistically enhancing the receptor‐specific cytotoxicity of targeted toxins. In short, this regular issue has a myriad of topics, which make the issue a must to read. Featured articles include: FAST TRACK: Blinded study determination of high sensitivity and specificity microchip electrophoresis‐SSCP/HA to detect mutations in the p53 gene (( 10.1002/elps.201100396 )) Online combination of reversed‐phase/reversed‐phase and porous graphitic carbon liquid chromatography for multicomponent separation of proteomics and glycoproteomics samples (( 10.1002/elps.201100092 )) Trace determination of perchlorate using electromembrane extraction and capillary electrophoresis with capacitively coupled contactless conductivity detection (( 10.1002/elps.201100279 )) Sensitivity enhancement in capillary electrophoresis‐mass spectrometry of anionic metabolites using a triethylamine‐containing background electrolyte and sheath liquid (( 10.1002/elps.201100271 )) Evaluation of the separation mechanism of electrokinetic chromatography with a microemulsion and cyclodextrins using NMR and molecular modeling (( 10.1002/elps.201100263 ))     

Cover Picture: Electrophoresis 20'2010

Issue no. 20 is a regular issue comprising 17 contributions distributed over 7 distinct parts. Part I has 3 research articles on particle analysis. Part II has 2 research articles dealing with hyphenated techniques such as CITP‐CZE and CE‐ESI‐MS. Part III reports a variety of methodologies and systems for proteins and proteomics that are described in 4 research articles. Gel DNA: theory and visualization are treated in 2 research articles making up part IV. Part V has 2 contributions that describe CE systems for water analysis. The following Part VI is on affinity and Immunoaffinity CE and has 2 research articles. The last 2 articles in this issue (Part VII) are on MEEKC and free flow electrophoresis. Featured articles include: Dielectrophoretically patterned carbon nanotubes to sort microparticles ((doi: 10.1002/elps.201000104 )) Analysis of 5‐methyltetrahydrofolate in human blood, serum and urine by on‐line coupling of capillary isotachophoresis and zone electrophoresis ((doi: 10.1002/elps.201000193 ))     

Cover Picture: Electrophoresis 11'09

Issue no. 11 is a regular issue consisting of 22 research articles distributed over 5 parts including: (i) Proteins and Proteomics, (ii) EKC and CEC, (iii) Detection and Preconcentration Approaches, (iv) Enantioseparation and (v) Methodologies and Assays. Selected articles are: Quantifying Western blots: pitfalls of densitometry (( 10.1002/elps.200800720 )) Light emitting diode‐induced chemiluminescence detection for capillary electrophoresis (( 10.1002/elps.200800708 )) Double sample preconcentration by in‐line coupled large volume single drop microextraction and sweeping in capillary electrophoresis (( 10.1002/elps.200800759 ))     

Cover Picture: Electrophoresis 22'2008

Regular issues provide a wide range of research and review articles covering all aspects of electrophoresis. Here you will find cutting‐edge articles on methods and theory, instrumentation, nucleic acids, CE and CEC, miniaturization and microfluidics, proteomics and two‐dimensional electrophoresis. Selected topics of issue 22 are: Microfluidics: Applications for analytical purposes in chemistry and biochemistry (( http://doi.wiley.com/10.1002/elps.200800121 )) Simultaneous laser‐induced fluorescence and retro‐reflected beam interference detection for CE (( http://doi.wiley.com/10.1002/elps.200800292 )) Quantitative Proteomics by Fluorescent Labeling of Cysteine Residues using a Set of Two Cyanine‐based or Three Rhodamine‐based Dyes (( http://doi.wiley.com/10.1002/elps.200800092 )) Chemometric resolution of fully overlapped capillary electrophoresis peaks: quantitation of carbamazepine in human serum in the presence of several interferences (( http://doi.wiley.com/10.1002/elps.200800400 )) Identification of inorganic ions in post‐blast explosive residues using portable capillary electrophoresis instrumentation and capacitively‐coupled contactless conductivity detection (( http://doi.wiley.com/10.1002/elps.200800226 ))     

Cover Picture: Electrophoresis 2'2010

Issue no. 2 has 19 contributions including one Fast Track contribution on “Thermophoresis of ssDNA”. The remaining 18 articles cover a wide variety of investigation involving nucleic acids, cells, viruses, interactive CE, detection methodologies in CE, enantioseparations, and studies on proteins and proteomics. Further selected articles include: Capillary electrophoresis of bone marrow stromal cells with uptake of heparin‐functionalized poly(lactide‐co‐glycolide) nanoparticles during differentiation towards neurons. (( 10.1002/elps.200900336 )) Ghrelin‐liposome interactions. Characterization of liposomal formulations of an acylated 28‐amino acid peptide using capillary electrophoresis. (( 10.1002/elps.200900394 ))     

Cover Picture: Electrophoresis 3'2010

Issue no. 3 is a regular issue with Emphasis on “Proteins and Proteomics”. The first part has 8 articles on proteins and proteomics covering various topics, e.g. preparative divergent flow IEF, multichannel gel electrophoresis, capillary gel electrophoresis, nanoparticle‐based CE of proteins, 2‐DE in a radial gel format, depletion of high abundance proteins, and proteomic investigation of fetal brain and lentil seed. The remaining 10 articles are concerned with nucleic acids, gene expression, methodologies and application. Featured articles include: Preparative divergent flow IEF without carrier ampholytes for separation of complex biological samples (( 10.1002/elps.200900484 )) SDS‐PAGE and two‐dimensional maps in a radial gel format (( 10.1002/elps.200900526 )) Analysis of Effect of Electrolyte Types on Electrokinetic Energy Conversion in Nanoscale Capillaries (( 10.1002/elps.200900409 )) A simple method to determine the surface charge in microfluidic channels (( 10.1002/elps.200900603 ))     

Cover Picture: Electrophoresis 21'2010

Issue no. 21 is a regular issue with "Emphasis on Nucleic Acids" and comprises 17 contributions distributed over 4 distinct parts. Part I is on nucleic acids and has 8 research articles on various aspects of nucleic acid research including an automated instrument for human STR identification, a high resolution chip‐CE assay for rapid detection of gene mutations and amplifications, a fluorescence‐based sequence‐specific primer PCR, pulsed field CE for RNA separation, methylated DNA immunoprecipitation sequencing, PCR‐SSCP, DNA methylation, and analysis of SNPs. Part II has 4 research articles dealing with studies on protein and proteomics analysis, e.g., microchip IEF, CE analysis of microheterogeneous glutelin subunits in rice, protein precipitation methods and prefractionation of plasma for proteomic analysis. Part III has 2 contributions that describe the separation of the enantiomers of DL‐isocitric acid by CE and the analysis of trace degradation products of S‐adenosylmethionine, which are decarboxylated diastereomers, by CE. The last 3 articles in this issue (Part IV) are on novel methodologies for facile interfacing of CE with MS, electrokinetic chromatography for the determination of contaminants and impurities of heparin samples, and particle electrophoresis. As such, issue no. 21 is packed with the latest developments and innovations in the field. Featured articles include: An automated instrument for Human STR identification: design, characterization, and experimental validation (doi: 10.1002/elps.201000305 )) Miniature flowing atmospheric‐pressure afterglow ion source for facile interfacing of capillary electrophoresis with mass spectrometry (doi: 10.1002/elps.201000350 )) Capillary electrophoresis for analysis of microheterogeneous glutelin subunits in rice (Oryza sativa L.). (doi: 10.1002/elps.201000333 ))     

Cover Picture: Electrophoresis 21'2009

Issue no. 21 is a regular issue with Emphasis on “Nucleic Acids”. The first part has 7 articles on nucleic acids covering various topics, e.g., sequencing, genotyping, PCR, insertion, mutation, etc. The remaining 11 articles are concerned with monoliths, pseudo‐phases, coating, and sample pretreatment such as derivatization and concentration. Selected articles are: Applications of MALDI‐TOF MS to large‐scale human mtDNA population‐based studies (( 10.1002/elps.200900294 )) Visual DNA as a diagnostic tool (( 10.1002/elps.200900273 )) Preliminary results for two‐dimensional separation with high performance thin‐layer chromatography and pressurized planar electrochromatography (( 10.1002/elps.200900471 ))