共查询到20条相似文献,搜索用时 46 毫秒
1.
Reconstitution of Nucleosome Demethylation and Catalytic Properties of a Jumonji Histone Demethylase
Carrie Shiau Michael J. Trnka Alen Bozicevic Idelisse Ortiz Torres Bassem Al-Sady Alma L. Burlingame Geeta J. Narlikar Danica Galonić Fujimori 《Chemistry & biology》2013,20(4):494-499
Highlights? Catalytic domain of JMJD2A (cJMJD2A) removes methyl marks in a distributive manner ? Homogeneously methylated nucleosomes were used as substrates ? Quantitative assay for nucleosome demethylation has been developed 相似文献
2.
N Thorne M Shen WA Lea A Simeonov S Lovell DS Auld J Inglese 《Chemistry & biology》2012,19(8):1060-1072
Highlights? A representative small molecule library contains 12% firefly luciferase inhibitors ? Competitive, noncompetitive, uncompetitive, and MAI inhibitors were identified ? For FLuc-RGAs 65% of FLuc inhibitors with known MOIs increased the luminescence signal ? Methods to identify FLuc inhibitors that interfere with assay results are described 相似文献
3.
Zhe Rui Min Ye Shuoguo Wang Kaori Fujikawa Bankole Akerele May Aung Heinz G. Floss Wenjun Zhang Tin-Wein Yu 《Chemistry & biology》2012,19(9):1116-1125
Highlights? Esm gene cluster resides on a highly unstable giant plasmid ? EsmA1 and A2 work together to promote the formation of phenazine-1,6-dicarboxylic acid ? Polyketide synthase enzymes EsmB1–B5 catalyze the unique methyl transfer ? Atypical polyketide synthase enzymes EsmD1–D3 catalyze the esterification. 相似文献
4.
Gen Tanaka Ikuhiko Nakase Yasunori Fukuda Ryo Masuda Shinya Oishi Kazuya Shimura Yoshimasa Kawaguchi Tomoka Takatani-Nakase Ülo Langel Astrid Gräslund Katsuya Okawa Masao Matsuoka Nobutaka Fujii Yasumaru Hatanaka Shiroh Futaki 《Chemistry & biology》2012,19(11):1437-1446
Highlights? CXCR4 was identified as a receptor to stimulate cellular uptake of R12 peptide ? Interaction with R12 stimulates internalization of CXCR4 via macropinocytosis ? SDF-1α and HIV-1 gp120 protein also induce macropinocytosis ? Macropinocytic uptake of HIV-1 diminished the infection of host cells 相似文献
5.
Jesmond Dalli Jeremy W. Winkler Romain A. Colas Hildur Arnardottir Chien-Yee C. Cheng Nan Chiang Nicos A. Petasis Charles N. Serhan 《Chemistry & biology》2013,20(2):188-201
Highlights? RvD3 accumulates in late inflammation resolution ? Aspirin triggers biosynthesis of RvD3 17R-epimer (AT-RvD3) that is proresolving ? Complete stereochemistry of RvD3 and AT-RvD3 are established ? RvD3 and AT-RvD3 govern leukocyte functions and local inflammatory responses 相似文献
6.
Highlights? Antibiotics can be classified by structural class based on biological fingerprints ? BioMAP predicts the presence of known compounds in natural product extracts ? BioMAP reveals compounds with unique antibacterial properties ? A naphthoquinone antibiotic with a unique carbon skeleton has been discovered 相似文献
7.
Highlights? Discovery of an azaphilone pathway in Aspergillus niger ? Six azaphilone compounds were isolated and characterized ? The biosynthesis involves convergent actions of an HR-PKS and an NR-PKS ? Hydroxylation by a monooxygenase promotes pyran-ring formation 相似文献
8.
Sara Christina Stolze Edgar Deu Farnusch Kaschani Nan Li Bogdan I. Florea Kerstin H. Richau Tom Colby Renier A.L. van der Hoorn Hermen S. Overkleeft Matthew Bogyo Markus Kaiser 《Chemistry & biology》2012,19(12):1546-1555
Highlights? A convergent chemical synthesis of symplostatin 4 (Sym4) and Sym4 derivatives is reported ? Sym4 irreversibly inhibits malarial falcipains in infected red blood cells ? Sym4's unusual methyl-methoxypyrrolinone group is required for nanomolar inhibition of falcipains 相似文献
9.
Mi-Yeon Jang Xiao-Ping Song Mathy Froeyen Philippe Marlière Eveline Lescrinier Jef Rozenski Piet Herdewijn 《Chemistry & biology》2013,20(3):416-423
Highlights? A highly modified nucleotide as substrate for polymerases ? The reversibility of the polymerase reaction at the template level ? Synthesis of a nucleoside with two anomeric centers 相似文献
10.
Andrew E. Howery Shelley Elvington Sherwin J. Abraham Kee-Hyun Choi Sierra Dworschak-Simpson Sabrina Phillips Christopher M. Ryan R. Lea Sanford Jonas Almqvist Kevin Tran Thomas A. Chew Ulrich Zachariae Olaf S. Andersen Julian Whitelegge Kimberly Matulef Justin Du Bois Merritt C. Maduke 《Chemistry & biology》2012,19(11):1460-1470
Highlights? OADS is a known small-molecule inhibitor of a CLC antiporter ? OADS specifically inhibits the ClC-ec1 antiporter but not the ClC-1 channel ? Photoaffinity labeling and mass spectrometry have localized OADS binding to two discrete sites ? The unique binding mode and lipid dependence of OADS suggest potential mechanisms of action 相似文献
11.
Vinayak Agarwal Elizabeth Pierce John McIntosh Eric W. Schmidt Satish K. Nair 《Chemistry & biology》2012,19(11):1411-1422
Highlights? Boundaries for cyanobactin maturation protease domains determined ? Structures of protease domains for cyanobactin maturation enzymes determined ? Additional structural motif in PatG assists in cyclizing transamidation ? Rational mutagenesis of active site leads to altered catalytic profiles for PatG 相似文献
12.
Takayuki Motoyama Toshiaki Hayashi Hiroshi Hirota Masashi Ueki Hiroyuki Osada 《Chemistry & biology》2012,19(12):1611-1619
Highlights? The terpendole biosynthetic gene cluster was isolated ? Terpendole E is a key biosynthetic intermediate of indole-diterpenes ? Terpendole E was overproduced by gene knockout of the bispecific enzyme TerP ? Indole-diterpene biosynthetic pathways can be classified into two groups 相似文献
13.
Highlights? A convenient synthesis of libraries of conformationally-constrained N-substituted oligoalanines ? A screen of such a library provided much higher affinity hits than a library of peptoids lacking c-alpha substitution ? Analysis of the isolated protein ligands demonstrated that the stereochemistry of the chiral centers was important ? These libraries should be a useful source of high affinity protein ligands 相似文献
14.
Keya Zhang Kathryn M. Nelson Karan Bhuripanyo Kimberly D. Grimes Bo Zhao Courtney C. Aldrich Jun Yin 《Chemistry & biology》2013,20(1):92-101
Highlights? Yeast surface display to engineer substrate recognition of adenylation (A) domains ? Synthesis of bisubstrate inhibitors for yeast selection of A domain library ? Identifying DhbE mutants specific for 3-hydroxybenzoic acid and 2-aminobenzoic acid ? Key residues in A domain for substrate recognition and transfer 相似文献
15.
Highlights? Populations of replicating RNAs were constructed by encoded combinatorial chemistry ? The RNAs evolve based on a heritable genotype and corresponding catalytic phenotype ? Genotype and phenotype are related by a user-specified genetic code ? The evolved enzymes included both highly active catalysts and molecular parasites 相似文献
16.
Highlights? Phylogenomics can be used to predict cognate ligands for TetR-family regulators ? KijR (SCO7719) regulates kijX (SCO7720) in response to the antibiotic kijanimicin ? kijX encodes a kijanimicin-resistance determinant ? KijX is a glycosyl hydrolase and inactivates kijanimicin through deglycosylation 相似文献
17.
Kimura H Sato Y Tajima Y Suzuki H Yukitake H Imaeda T Kajino M Oki H Takizawa M Tanida S 《Chemistry & biology》2010,17(12):401-1294
Highlights? ARE activation is involved in cellular protection against oxidative stress ? MIF is a ubiquitous protein with conserved nucleophilic Pro1 in a hydrophobic pocket ? BTZO-1 selectively binds to MIF, and its binding required the intact N-terminal Pro1 ? Binding of BTZO-1 to MIF leads to the ARE activation in cardiomyocytes. 相似文献
18.
Härle J Günther S Lauinger B Weber M Kammerer B Zechel DL Luzhetskyy A Bechthold A 《Chemistry & biology》2011,18(4):520-530
Highlights? 106 rationally designed chimeras were expressed in vivo and screened by HPLC-MS analysis ? One 12-amino acid protein segment is crucial for O- or C-glycosylation ? The sugar-acceptor postioning determines O- or C-glycosidic bond formation ? Ala substitutions revealed the key spots for glycosylation 相似文献
19.
Highlights? Recycling of genetic material is important for the origins of life ? Simulation studies show recycling leads to the selection of genotypes from a pool ? Recycling can lead to the sudden emergence of a high-fitness RNA genotype ? Experimental studies with the Azoarcus ribozyme show fragment recycling 相似文献
20.
Nishikimi A Uruno T Duan X Cao Q Okamura Y Saitoh T Saito N Sakaoka S Du Y Suenaga A Kukimoto-Niino M Miyano K Gotoh K Okabe T Sanematsu F Tanaka Y Sumimoto H Honma T Yokoyama S Nagano T Kohda D Kanai M Fukui Y 《Chemistry & biology》2012,19(4):488-497
Highlights? CPYPP binds to DOCK2 DHR-2 domain and inhibits its catalytic activity ? CPYPP inhibits DOCK2-mediated Rac activation in cells ? The structural features of CPYPP required for its inhibitory effect were revealed ? CPYPP inhibits lymphocyte migration and activation in vitro and in vivo 相似文献