A Versatile Approach Towards Nucleobase‐Modified Aptamers |
| |
| Authors: | Fabian Tolle Dr Gerhard M Brändle Daniel Matzner Prof Dr Günter Mayer |
| |
| Affiliation: | Life and Medical Sciences Institute, University of Bonn, Gerhard‐Domagk‐Str. 1, 53121 Bonn (Germany) |
| |
| Abstract: | A novel and versatile method has been developed for modular expansion of the chemical space of nucleic acid libraries, thus enabling the generation of nucleobase‐modified aptamers with unprecedented recognition properties. Reintroduction of the modification after enzymatic replication gives broad access to many chemical modifications. This wide applicability, which is not limited to a single modification, will rapidly advance the application of in vitro selection approaches beyond what is currently feasible and enable the generation of aptamers to many targets that have so far not been addressable. |
| |
| Keywords: | aptamers GFP molecular evolution nucleic acids SELEX |
|
|
