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Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases |
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| Authors: | Zuzana Vaníková Prof Dr Michal Hocek |
| Affiliation: | 1. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences & IOCB Research Center, Flemingovo nám. 2, 16610 Prague 6 (Czech Republic) http://www.uochb.cas.cz/hocekgroup;2. Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 12843 Prague 2 (Czech Republic) |
| Abstract: | 5‐(2‐Nitrobenzyl)oxymethyl]‐2′‐deoxyuridine 5′‐O‐triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5‐hydroxymethyluracil‐modified nucleic acids, which were fully recognized and cleaved by restriction enzymes. |
| Keywords: | DNA DNA polymerase nucleotides photocaging protective groups |
