Significance of Branching for Transfection: Synthesis of Highly Branched Degradable Functional Poly(dimethylaminoethyl methacrylate) by Vinyl Oligomer Combination |
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| Authors: | Tianyu Zhao Hong Zhang Dr Ben Newland Ahmed Aied Dr Dezhong Zhou Dr Wenxin Wang |
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| Affiliation: | 1. Charles Institute of Dermotology, University College Dublin, Dublin (Ireland) http://www.wenxinwang.ie;2. School of Materials Science and Engineering, Tianjin Universiy, Tianjin (China);3. Leibnitz‐Institut für Polymerforschung, Dresden (Germany);4. Network of Excellence for Functional Biomaterials, National University of Ireland, Galway (Ireland) |
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| Abstract: | A series of degradable branched PDMAEMA copolymers were investigated with the linear PDMAEMA counterpart as gene‐delivery vectors. The branched PDMAEMA copolymers were synthesized by controlled radical cross‐linking copolymerization based on the “vinyl oligomer combination” approach. Efficient degradation properties were observed for all of the copolymers. The degree of branching was found to have a big impact on performance in transfection when tested on different cell types. The product with the highest degree of branching and highest degree of functionality had a superior transfection profile in terms of both transfection capability and the preservation of cell viability. These branched PDMAEMA copolymers show high potential for gene‐delivery applications through a combination of the simplicity of their synthesis, their low toxicity, and their high performance. |
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| Keywords: | branched polymers copolymerization degradable polymers transfection vinyl oligomer |
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