Proteomic profiling of human sera for discovery of potential biomarkers to monitor abstinence from alcohol abuse |
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| Authors: | Xianyin Lai Suthat Liangpunsakul Kaigang Li Frank A Witzmann |
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| Affiliation: | 1. Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, IN, USA;2. Department of Cellular & Integrative Physiology, Indiana University School of Medicine, IN, USA;3. Department of Medicine, Division of Gastroenterology/Hepatology, Indiana University School of Medicine, IN, USA;4. Roudebush Veterans Administration Medical Center, IN, USA;5. Department of Applied Health Science, Indiana University School of Public Health, Bloomington, IN, USA |
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| Abstract: | Although numerous biomarkers or biomarker candidates have been discovered to detect levels of drinking and intervals of time after last drinking episode, only a few biomarkers have been applied to monitor abstinence in a longer interval (≥6 wks) from alcohol abuse. Considering sample sources, sensitivity, and specificity, new biomarkers from blood with better accuracy are needed. To address this, serum proteomic profiles were compared between pre‐ and post‐ treatment samples from subjects seeking treatment for alcohol abuse and dependence in an intensive 6wk daily outpatient program using high‐abundance plasma protein immunodepletion and LC‐MS/MS techniques. Protein identification, quantification, candidate biomarker selection, and prioritization analyses were carried out. Among the 246 quantified serum proteins, abundance of 13 and 45 proteins in female and male subjects were significantly changed (p ≤ 0.05), respectively. Of these biomarker candidate proteins, 2 (female) and 8 (male) proteins were listed in category 1, with high area under the receiver operating characteristic curve, sensitivity, specificity, and fold change. In summary, several new biomarker candidates have been identified to monitor abstinence from alcohol abuse. |
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| Keywords: | Alcohol Abuse Biomarker MS/MS Proteomics |
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