Quantitative analysis of 3‐OHB[a]P and (+)‐anti‐BPDE as biomarkers of B[a]P exposure in rats |
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| Authors: | Qi Lin Liu Xiao‐Chen Yang Bo Liu Na Shi Min Chen Gang Liu Hui Li Fa‐Sheng |
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| Affiliation: | 1. College of Medical Laboratory, Dalian Medical University, Dalian, LiaoNing Province, China;2. Harbin Infectious Disease Hospital, Harbin 150036, HeiLongJiang Province, China;3. College of Pharmacy, Washington State University, Spokane, WA, USA |
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| Abstract: | The aim of this study was to develop an analytical method for the determination the levels of metabolites of benzoa]pyrene (Ba]P), 3‐hydroxybenzo(a)pyrene (3‐OHBa]P) and (+)‐anti‐benzo(a)pyrene diol‐epoxide (+)‐anti‐BPDE, combined with DNA to form adducts], in rat blood and tissues exposed to Ba]P exposure by high‐performance liquid chromatography with fluorescence detection (HPLC/FD), and to investigate the usefulness of 3‐OHBa]P and (+)‐anti‐BPDE as markers of intragastrical exposure to Ba]P in rats. The levels of 3‐OH‐Ba]P and Ba]P‐tetrol I‐1 released after acid hydrolysis of (+)‐anti‐BPDE in the samples were measured by HPLC/FD. The calibration curves were linear (r2 > 0.9904), and the lower limit of quantification ranged from 0.34 to 0.45 ng/mL for 3‐OHBa]P and from 0.43 to 0.58 ng/mL for (+)‐anti‐BPDE. The intra‐ and inter‐day stability assay data suggested that the method is accurate and precise. The recoveries of 3‐OHBa]P and (+)‐anti‐BPDE were in the ranges of 73.6 ± 5.0 to 116.5 ± 6.3% and 73.3 ± 8.5 to 141.2 ± 13.8%, respectively. A positive correlation was found between the concentration of intragastrical Ba]P and the concentrations of 3‐OH‐Ba]P and (+)‐anti‐BPDE in the blood and in most of the tissues studied, except for the brain and kidney, which showed no correlation between Ba]P and 3‐OHBa]P and between Ba]P and (+)‐anti‐BPDE, respectively. A sensitive, reliable and rapid HPLC/FD was developed and validated for analysis of 3‐OHBa]P and (+)‐anti‐BPDE in rat blood and tissues. There was a positive correlation between the concentration of 3‐OHBa]P or (+)‐anti‐BPDE in the blood and the concentration of 3‐OHBa]P or (+)‐anti‐BPDE in the most other tissues examined. The concentration of 3‐OHBa]P or (+)‐anti‐BPDE in the blood could be used as an indicator of the concentration of 3‐OHBa]P or (+)‐anti‐BPDE in the other tissues in response to Ba]P exposure. These results demonstrate that 3‐OHBa]P and (+)‐anti‐BPDE are potential biomarkers of Ba]P exposure, which would also be useful to assess the carcinogenic risks from Ba]P exposure. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | 3-hydroxy-benzo[a]pyrene (+)-anti-benzo[a]pyrene-diol-epoxide high performance liquid chromatography/Fluorescence Detection rats |
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