Bifunctional Fluorescent Probe for Sequential Sensing of Thiols and Primary Aliphatic Amines in Distinct Fluorescence Channels |
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| Authors: | Meiyu Huang Prof?Dr Lingliang Long Ning Wang Xiangqi Yuan Siyu Cao Aihua Gong Prof?Dr Kun Wang |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu, P.R. China;2. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, P.R. China |
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| Abstract: | Thiols and primary aliphatic amines (PAA) are ubiquitous and extremely important species in biological systems. They perform significant interplaying roles in complex biological events. A single fluorescent probe differentiating both thiols and PAA can contribute to understanding the intrinsic inter‐relationship of thiols and PAA in biological processes. Herein, we rationally constructed the first fluorescent probe that can respond to thiols and PAA in different fluorescence channels. The probe exhibited a high selectivity and sensitivity to thiols and PAA. In addition, it displayed sequential sensing ability when the thiols and PAA coexisted. The application experiments indicated that the probe can be used for sensing thiols and PAA in human blood serum. Moreover, the fluorescence imaging of endogenous thiols and PAA as well as antihypertensive drugs captopril and amlodipine in living cells were successfully conducted. |
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| Keywords: | imaging agents dyes/pigments fluorescence spectroscopy primary aliphatic amines sensors thiols |
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