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| 乙酰鸟氨酸脱乙酰酶固定化细胞拆分D,L-缬氨酸 | |
| 引用本文： | 张飞,熊吉滨,刘均忠,刘鹏刚,焦庆才.乙酰鸟氨酸脱乙酰酶固定化细胞拆分D,L-缬氨酸[J].高等学校化学学报,2009,30(8):1577-1580. |
| 作者姓名： | 张飞 熊吉滨 刘均忠 刘鹏刚 焦庆才 |
| 作者单位： | 1. 南京大学生命科学学院,医药生物技术国家重点实验室,南京,210093;包头轻工职业技术学院生物工程系,包头,014043 2. 南京大学生命科学学院,医药生物技术国家重点实验室,南京,210093 |
| 摘 要： | 报道了一种利用具有乙酰鸟氨酸脱乙酰酶活性的固定化细胞拆分D,L-缬氨酸的新方法. 该酶促反应最适条件: pH=6, 反应温度50 ℃, 底物N-乙酰-D,L-缬氨酸浓度200 mmol/L, 固定化细胞用量0.2 g/mL(或100 U/mL). 0.1 mmol/L CoCl2条件对该酶促反应有显著的激活作用. 在以上条件下反应2～3 h, 测得产物L-缬氨酸浓度95 mmol/L. 该固定化细胞连续10次使用, 平均转化率为90.8%(以N-乙酰-L-缬氨酸计), 显示出了良好的工业化应用前景. |
| 关 键 词： | 乙酰鸟氨酸脱乙酰酶 拆分 固定化细胞 D L-缬氨酸 |
| 收稿时间： | 2008-12-30 |
Chiral Resolution of D,L-Valine by Immobilized Cells with Acetylornithine Deacetylase Activity |
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| ZHANG Fei,XIONG Ji-Bing,LIU Jun-Zhong,LIU Peng-Gang,JIAO Qing-Cai.Chiral Resolution of D,L-Valine by Immobilized Cells with Acetylornithine Deacetylase Activity[J].Chemical Research In Chinese Universities,2009,30(8):1577-1580. | |
| Authors: | ZHANG Fei XIONG Ji-Bing LIU Jun-Zhong LIU Peng-Gang JIAO Qing-Cai |
| Affiliation: | 1. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; 2. Baotou Light Industry Vocational Technical College, Baotou 014043, China |
| Abstract: | D-Amino acids are widely used intermediates in pharmacy. D-Amino acids can be produced by chiral separation of D,L-amino acids racemate. The stereospecific hydrolysis of N-acetyl-D,L-amino acids by microbial enzymys is one of the most extensively used procedures in optical resolution of D,L-amino acids. In this report, a new method of chiral resolution of D,L-valine by immobilized cells with acetylornithine deacetylase activity was developed. The optimum reaction conditions were 200 mmol/L N-acetyl-D,L-valine and 0.2 g/mL(or 100 U/mL) of immobilized cells with 0.1 mmol/L CoCl2 as activator at pH=6. After 2—3 h incubation at 50 ℃, 95 mmol/L L-valine was obtained. In continuous use of immobilized cells for 10 times, the average conversion rate against N-acetyl-L-valine was 90.8%. The results display great potential in industrialization. This is the first report of chiral resolution of D,L-valine by acetylornithine deacetylase(ArgE). |
| Keywords: | D Acetylornithine deacetylase" target="_blank">L-Aline')" href="#">Acetylornithine deacetylase Chiral resolution Immobilized cell D L-Aline |
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