| 用荧光猝灭和荧光加强两种理论研究喹诺酮类新药与白蛋白的作用 |
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| 引用本文： | 杨曼曼,席小莉,杨频.用荧光猝灭和荧光加强两种理论研究喹诺酮类新药与白蛋白的作用[J].高等学校化学学报,2006,27(4):687-691. |
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| 作者姓名： | 杨曼曼 席小莉 杨频 |
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| 作者单位： | 山西大学化学化工学院分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006 |
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| 基金项目： | 中国科学院资助项目;山西省青年基金 |
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| 摘 要： | 应用荧光加强和荧光猝灭两种理论公式, 对四种喹诺酮类药物与人血清和牛血清白蛋白的作用作进行了对比研究, 对药物与白蛋白的结合特点和通常的表征量(解离常数、 猝灭常数、 猝灭效率、 能量转移效率、 给体 受体作用距离等)进行了深入地分析; 在白蛋白与药物结合类型上, 四种药物对HSA和BSA的猝灭实验结果表明， 这种由给体-受体结合引起的猝灭作用类型不是由生物大分子血清白蛋白单方面决定的, 而是由血清白蛋白与药物、 即给体与受体两者的分子结构和相互匹配共同决定的.
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| 关 键 词： | 荧光猝灭和加强 人血清白蛋白(HSA) 牛血清白蛋白(BSA) 喹诺酮类药物 |
| 文章编号： | 0251-0790（2006）04-0687-05 |
| 收稿时间： | 05 24 2005 12:00AM |
| 修稿时间： | 2005-05-24 |
Studies on Interaction of New Medicine of Quinolone-Class with HSA and BSA by Using Fluorescence Enhancement and Fluorescence Quenching Theory |
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| YANG Man-Man,XI Xiao-Li,YANG Pin.Studies on Interaction of New Medicine of Quinolone-Class with HSA and BSA by Using Fluorescence Enhancement and Fluorescence Quenching Theory[J].Chemical Research In Chinese Universities,2006,27(4):687-691. |
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| Authors: | YANG Man-Man XI Xiao-Li YANG Pin |
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| Affiliation: | Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China |
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| Abstract: | By using the two theoriesfluorescence enhancement and fluorescence quenching,we studied and compared the inter action of four kinds of Quinolone-class-medicines with HSA and BSA,then analysed deeply the binding speciality of medicine and albumin and usual characterization constants: dissociation constant,quenching constant,quenching efficiency,energy-transfer efficiency,the distance between donor and accepter.About binding type of albumin with medicine,through the quenching experiment result of the four kinds of medicines with HSA and BSA,we found that this quenching action type caused by the binding of donor between acceptor was not determined by the oneself-biomacromolecule BSA,but together determined by the molecular structure of BSA and medicine,donor and acceptor,and their match with each other. |
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| Keywords: | Fluorescence enhancement and quenching HSA BSA Quinolone-class-medicine |
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