| 介孔材料MCFs的合成及组装青霉素酰化酶的性质研究 |
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| 引用本文： | 高波,陈静,朱广山,傅学奇,王春雷,裘式伦.介孔材料MCFs的合成及组装青霉素酰化酶的性质研究[J].高等学校化学学报,2004,25(11):1998-2000. |
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| 作者姓名： | 高波 陈静 朱广山 傅学奇 王春雷 裘式伦 |
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| 作者单位： | 1. 吉林大学化学学院无机合成与制备化学国家重点实验室, 长春130012;
2. 吉林大学生命科学学院, 长春130012 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 9873 0 17和 2 0 10 10 0 4)，国家“九七三”计划项目 (批准号 :G2 0 0 0 0 775 0 7)资助 |
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| 摘 要： | 介孔材料由于具有纳米级规则孔道和巨大的比表面积而在催化、吸附及分离等方面存在较大的应用价值．近年来，由介孔分子筛如MCM-41和SBA-15州等组装功能性材料已成为研究的热点．酶作为高效催化剂有许多优点，但在溶液中易失活，使用后无法回收，有的酶在溶液中还存在自水解问题：将酶组装在介孔材料中制成固定化酶则可解决上述问题．目前已成功地将辣根过氧化物酶
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| 关 键 词： | 介孔材料MCFs 青霉素酰化酶 活性 稳定性 |
| 文章编号： | 0251-0790(2004)11-1998-03 |
| 收稿时间： | 2003-12-16 |
Immobilization and Property of Penicillin G Acylase in the Channel of Silica Mesoporous Material MCFs |
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| GAO Bo ,CHEN Jing,ZHU Guang-Shan,FU Xue-Qi,WANG Chun-Lei,QIU Shi-Lun.Immobilization and Property of Penicillin G Acylase in the Channel of Silica Mesoporous Material MCFs[J].Chemical Research In Chinese Universities,2004,25(11):1998-2000. |
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| Authors: | GAO Bo CHEN Jing ZHU Guang-Shan FU Xue-Qi WANG Chun-Lei QIU Shi-Lun |
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| Affiliation: | 1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Changchun 130023, China;
2. College of Life Science, Jilin University, Changchun 130023, China |
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| Abstract: | Silica mesoporous material MCFs with 16.0 nm pore sizes was prepared by using non-ionic block copolymers and the swelling agents, and was used as the support for the immobilization of enzyme. Penicillin G acylase, an enzyme, was assembled in the channel of MCFs by immersion method. The activity and stability of immobilized penicillin G acylase were studied. It was found that the activity and stability of the immobilized penicillin G acylase increased significantly compared to those of free enzyme. The optimum reaction temperature is 60 ℃. After incubation at 60 ℃ for 1 h, the activity of these immobilized penicillin G acylase remains 69%. These results showed that thermostability and durability on heating of the immobilized penicillin G acylase in MCFs was improved remarkably. The silica mesoporous material MCFs with 3-dimensional channel structure is a good support for the immobilization of enzyme. |
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| Keywords: | Mesoporous material MCFs Penicillin G acylase Activity Stability |
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