Vibronic Structures in Absorption and Fluorescence Spectra of Firefly Oxyluciferin in Aqueous Solutions |
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| Authors: | Miyabi Hiyama Yoshifumi Noguchi Hidefumi Akiyama Kenta Yamada Nobuaki Koga |
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| Affiliation: | 1. Institute for Solid State Physics, The University of Tokyo, Kashiwa, Chiba, Japan;2. Graduate School of Information Science, Nagoya University, Nagoya, Japan |
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| Abstract: | To elucidate the factors determining the spectral shapes and widths of the absorption and fluorescence spectra for keto and enol oxyluciferin and their conjugate bases in aqueous solutions, the intensities of vibronic transitions between their ground and first electronic excited states were calculated for the first time via estimation of the vibrational Franck–Condon factors. The major normal modes, overtones and combination tones in absorption and fluorescence spectra are similar for all species. The theoretical full widths at half maximum of absorption spectra are 0.4–0.7 eV and those for the fluorescence spectra are 0.4–0.5 eV, except for phenolate‐keto that exhibits exceptionally sharp peak widths due to the dominance of the 0–0′ or 0′–0 band. These spectral shapes and widths explain many relevant features of the experimentally observed spectra. |
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