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Evaluation of Some Redox Mediators in the Design of Reagentless Amperometric Glucose Biosensor
Authors:Asta Kausaite‐Minkstimiene  Viktor Mazeiko  Almira Ramanaviciene  Yasemin Oztekin  Ali Osman?Solak  Arunas Ramanavicius
Affiliation:1. Nanotechnas – Centre of Nanotechnology and Material Science, Vilnius University, Naugarduko 24, LT‐03225 Vilnius, Lithuania;2. Division of Immunotechnology, State Research Institute Centre for Innovative Medicine, Zygimant? 9, LT‐01102 Vilnius, Lithuania;3. Department of Chemistry, Faculty of Science, Selcuk University, Konya 42075, Turkey;4. Department of Chemistry, Faculty of Science, Ankara University, Ankara 06100, Turkey;5. Department of Chemical Engineering, Kyrgyz‐Turk Manas University, Bishkek 720044,Kyrgyzstan;6. Laboratory of NanoBioTechnology, Department of Materials Science and Electronics, Institute of Semiconductor Physics, State Scientific Research Institute Centre for Physical Sciences and Technology, A. Go?tauto 9, LT‐01108 Vilnius, Lithuania
Abstract:The influence of redox mediators (tetrathiafulvalene (TTF), tetrathiafulvalene‐tetracyanoquinodimethane complex (TTF‐TCNQ), phenazine methosulfate (PMS), 5,6‐diamino‐1,10‐phenonthroline (5,6‐DAP), potassium ferrocyanide (K4Fe(CN)6]), methylene blue (MB) and toluidine blue (TB)) on the response of amperometric glucose biosensor was investigated in this research. Graphite rod electrode (GRE) modified by adsorbed mediator and glucose oxidase (GOx) cross‐linked with glutaraldehyde was served as working electrode. The highest amperometric signals were observed by using TTF and TTF‐TCNQ as mediators. The impact of amount of immobilized GOx, an optimal pH region for operation, stability and reproducibility of the analytic signal of designed biosensor using these mediators were investigated and discussed.
Keywords:Amperometric glucose biosensor  Immobilized glucose oxidase  Electron transfer mediator  Methylene blue  Toluidine blue
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