Development of an Electrochemical Biosensor for the Rapid Detection of Cholera Toxin Based on Air Stable Lipid Films with Incorporated Ganglioside GM1 Using Graphene Electrodes |
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| Authors: | Stephanos Karapetis Georgia‐Paraskevi Nikoleli Christina G Siontorou Dimitrios P Nikolelis Nikolaos Tzamtzis Nikolas Psaroudakis |
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| Affiliation: | 1. Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept 1, Chemical Sciences, National Technical University of Athens, Athens, Greece;2. Laboratory of Simulation of Industrial Processes, Department of Industrial Management and Technology, School of Maritime and IndustryUniversity of Piraeus;3. +302107274754;4. Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Athens, Greece;5. Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Athens, Greece |
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| Abstract: | The present work describes a miniaturized potentiometric cholera toxin sensor on graphene nanosheets with incorporated lipid films. Ganglioside GM1, the natural cholera toxin receptor, immobilized on the stabilized lipid films, provided adequate selectivity for detection over a wide range of toxin concentrations, fast response time of ca. 5 min, and detection limit of 1 nM. The proposed sensor is easy to construct and exhibits good reproducibility, reusability, selectivity, long shelf life and high sensitivity of ca. 60 mV/decade of toxin concentration. The method was implemented and validated in lake water samples. This novel ultrathin film technology is currently adapted to the rapid detection of other toxins that could be used in bioterrorism. |
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| Keywords: | Stabilized lipid films Cholera toxin biosensor Graphene electrodes Gangliosides Electroanalysis |
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