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Orchestrating the Biosynthesis of an Unnatural Pyrrolysine Amino Acid for Its Direct Incorporation into Proteins Inside Living Cells |
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| Authors: | Michael Ehrlich Michael J Gattner Bastien Viverge Johanna Bretzler Dr David Eisen Michael Stadlmeier Dr Milan Vrabel Prof Dr Thomas Carell |
| Affiliation: | 1. Center for Integrative Protein Science at the Department of Chemistry, Ludwig‐Maximilians Universit?t München, Butenandtstrasse 5–13, 81377 Munich (Germany);2. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo na'm. 2, 166 10, Prague (Czech Republic) |
| Abstract: | We here report the construction of an E. coli expression system able to manufacture an unnatural amino acid by artificial biosynthesis. This can be orchestrated with incorporation into protein by amber stop codon suppression inside a living cell. In our case an alkyne‐bearing pyrrolysine amino acid was biosynthesized and incorporated site‐specifically allowing orthogonal double protein labeling. |
| Keywords: | amber suppression bioorganic chemistry pyrrolysine synthetic biology unnatural amino acid |
