Determination of donepezil in serum samples using molecularly imprinted polymer nanoparticles followed by high‐performance liquid chromatography with ultraviolet detection |
| |
| Authors: | Mehdi Rajabnia Khansari Shahrzad Bikloo Sara Shahreza |
| |
| Affiliation: | 1. Research Center, School of Chemical Engineering, Iran University of Science and Technology, Tehran, Iran;2. Research Center, Faculty of Pharmacy, Shaheed Beheshtee University of Medical Sciences, Tehran, Iran |
| |
| Abstract: | A molecularly imprinted polymer designed for the selective extraction of donepezil from serum samples was synthesized using a noncovalent molecular imprinting approach. The molecularly imprinted polymer was evaluated chromatographically and then its affinity for donepezil was confirmed by solid‐phase extraction. The optimal conditions for solid‐phase extraction were provided by cartridge conditioning using acidified water purified from a Milli‐Q system, sample loading under basic aqueous conditions, clean‐up using acetonitrile, and elution with methanol/tetrahydrofuran. Desirable molecular recognition properties of the molecularly imprinted polymer led to good donepezil recoveries (90–102%). The data indicated that the imprinted polymer has a perfect selectivity and affinity for donepezil and could be used for selective extraction and analysis of donepezil in human serum. |
| |
| Keywords: | Donepezil Molecularly imprinted polymers Serum samples Solid‐phase extraction |
|
|
