Qualitative analysis of major constituents from Xue Fu Zhu Yu Decoction using ultra high performance liquid chromatography with hybrid ion trap time‐of‐flight mass spectrometry |
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| Authors: | Chunyan Fu Zian Xia Yonghui Liu Hongmei Lu Zhimin Zhang Xiaqiong Fan |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha, P. R. China;2. Department of Pharmacy, Shaoyang Medical College Level Specialty School, Hunan, Shaoyang, P. R. China;3. Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Hunan, Changsha, P. R. China |
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| Abstract: | Xue Fu Zhu Yu Decoction, a famous formula that has been used for treating many blood stasis‐caused diseases for many centuries, comprises 11 kinds of traditional Chinese medicines. A convenient, efficient, and rapid analytical method was developed to simultaneously determine the major compounds in this decoction. An ultra‐high performance liquid chromatography with hybrid ion trap time‐of‐flight mass spectrometry method was used to rapidly separate and detect the major constituents of the decoction. Using this technique, we identified or tentatively identified 34 compounds, including 21 flavonoids, 5 terpenoids, 3 organic acids, 2 lactones, 1 alkaloid, 1 amino acid, and 1 cyanogenic glycoside. The MS analysis of these constituents was described in detail. Findings may contribute to future metabolic and pharmacokinetic studies of this medicine. |
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| Keywords: | Major constituents Qualitative analysis Traditional Chinese medicine Ultra‐high performance liquid chromatography Xue Fu Zhu Yu Decoction |
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